(Circular) (Six-base) MAPSORT of: pFN21AB7570.seq Check: 835 from: 1 to: 5542 pFN21AB7570 5542 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2521 2521 Size: 5542 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3352 278 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 1937 Size: 948 4594 Acc65I G'GTAC_C Cuts at: 2863 2863 Size: 5542 AclI AA'CG_TT Cuts at: 1563 3669 4042 1563 Size: 2106 373 3063 Fragments arranged by size: 3063 2106 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2685 3605 4989 5257 1632 Size: 1053 920 1384 268 1917 Fragments arranged by size: 1917 1384 1053 920 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5345 AflIII A'CryG_T Cuts at: 1203 4441 5418 1203 Size: 3238 977 1327 Fragments arranged by size: 3238 1327 977 AgeI A'CCGG_T Cuts at: 4373 4373 Size: 5542 AhdI GACnn_n'nnGTC Cuts at: 4271 4271 Size: 5542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2314 1184 Size: 1130 4412 AlwNI CAG_nnn'CTG Cuts at: 2293 2512 3285 4857 2293 Size: 219 773 1572 2978 Fragments arranged by size: 2978 1572 773 219 ApaLI G'TGCA_C Cuts at: 3599 4755 3599 Size: 1156 4386 ApoI r'AATT_y Cuts at: 1493 2456 2851 3077 3229 5338 1493 Size: 963 395 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 4096 160 Size: 872 3064 1606 Fragments arranged by size: 3064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 AvaI C'yCGr_G Cuts at: 1870 1943 2275 2867 1870 Size: 73 332 592 4545 Fragments arranged by size: 4545 592 332 73 BamHI G'GATC_C Cuts at: 2872 3239 2872 Size: 367 5175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2863 4318 618 Size: 325 217 627 1076 1455 1842 Fragments arranged by size: 1842 1455 1076 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2195 2712 2733 2861 1391 Size: 645 159 517 21 128 4072 Fragments arranged by size: 4072 645 517 159 128 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2543 928 Size: 971 644 3927 Fragments arranged by size: 3927 971 644 BbvCI CC'TCA_GC Cuts at: 2024 2328 2024 Size: 304 5238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3733 3767 1903 Size: 34 50 34 1712 34 3678 Fragments arranged by size: 3678 1712 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3434 4644 3434 Size: 1210 4332 BclI T'GATC_A Cuts at: 1548 2229 1548 Size: 681 4861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4153 136 Size: 107 122 71 631 605 2481 1525 Fragments arranged by size: 2481 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5537 5537 Size: 5542 BlpI GC'TnA_GC Cuts at: 2030 2959 2030 Size: 929 4613 Bme1580I G_kGCm'C Cuts at: 1790 2150 3603 4759 1790 Size: 360 1453 1156 2573 Fragments arranged by size: 2573 1453 1156 360 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5542 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3273 4231 442 Size: 416 53 83 399 24 1856 958 1753 Fragments arranged by size: 1856 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5542 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2113 2338 2113 Size: 225 5317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2726 4202 1239 Size: 1229 258 1476 2579 Fragments arranged by size: 2579 1476 1229 258 Bpu10I CC'TnA_GC Cuts at: 2024 2328 2024 Size: 304 5238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3667 4532 4830 3667 Size: 865 298 4379 Fragments arranged by size: 4379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 2326 4205 882 Size: 617 600 227 1879 2219 Fragments arranged by size: 2219 1879 617 600 227 BsaAI yAC'GTr Cuts at: 493 1204 2759 3333 493 Size: 711 1555 574 2702 Fragments arranged by size: 2702 1555 711 574 BsaBI GATnn'nnATC Cuts at: 5536 5536 Size: 5542 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3349 3731 4366 275 Size: 53 83 186 936 1816 382 635 1451 Fragments arranged by size: 1816 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3975 4373 4647 4794 5205 3975 Size: 398 274 147 411 4312 Fragments arranged by size: 4312 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5317 Fragments arranged by size: 5317 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2741 1186 Size: 1024 531 3987 Fragments arranged by size: 3987 1024 531 BseYI C'CCAG_C Cuts at: 4745 5241 4745 Size: 496 5046 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4969 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2992 3753 4781 1540 Size: 275 186 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 2733 3603 3688 4759 2036 Size: 697 870 85 1071 2819 Fragments arranged by size: 2819 1071 870 697 85 BsmI GAATG_Cn' Cuts at: 2179 3053 3146 2179 Size: 874 93 4575 Fragments arranged by size: 4575 874 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2363 2363 Size: 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2150 2195 2712 2733 2861 3603 Size: 399 246 114 45 517 21 128 742 Cuts at: 3603 3688 4759 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 246 128 114 85 45 21 BspEI T'CCGG_A Cuts at: 5205 5205 Size: 5542 BspHI T'CATG_A Cuts at: 2079 3431 5161 2079 Size: 1352 1730 2460 Fragments arranged by size: 2460 1730 1352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2521 2897 844 Size: 1273 404 376 3489 Fragments arranged by size: 3489 1273 404 376 BsrBI CCG'CTC Cuts at: 1394 1823 2218 3429 1394 Size: 429 395 1211 3507 Fragments arranged by size: 3507 1211 429 395 BsrDI GCAATG_nn' Cuts at: 65 1248 4037 4211 65 Size: 1183 2789 174 1396 Fragments arranged by size: 2789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4186 4373 1700 Size: 110 2376 187 2869 Fragments arranged by size: 2869 2376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5542 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5542 BssSI C'ACGA_G Cuts at: 3602 4614 3602 Size: 1012 4530 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5542 BstBI TT'CG_AA Cuts at: 4346 4346 Size: 5542 BstEII G'GTnAC_C Cuts at: 1184 2308 1184 Size: 1124 4418 BstYI r'GATC_y Cuts at: 1972 2091 2872 3239 3624 3641 5093 5179 5191 Size: 119 781 367 385 17 1452 86 12 Cuts at: 5191 5537 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 781 385 367 346 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4990 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4803 Fragments arranged by size: 4803 683 56 BtsI GCAGTG_nn' Cuts at: 2008 3058 3852 3872 2008 Size: 1050 794 20 3678 Fragments arranged by size: 3678 1050 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5507 DraI TTT'AAA Cuts at: 2846 2846 Size: 5542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4549 809 Size: 507 3233 1802 Fragments arranged by size: 3233 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2989 3878 Size: 54 1425 50 275 18 211 948 889 Cuts at: 3878 5507 8 Size: 1629 43 Fragments arranged by size: 1629 1425 948 889 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2989 1537 Size: 275 1177 4090 Fragments arranged by size: 4090 1177 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 2661 2824 3472 1353 Size: 1293 15 163 648 3423 Fragments arranged by size: 3423 1293 648 163 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4129 4503 4649 387 Size: 1064 138 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2034 2731 2034 Size: 697 4845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2685 2726 3605 4202 4989 5257 Size: 393 836 217 41 879 597 787 268 Cuts at: 5257 1239 Size: 1524 Fragments arranged by size: 1524 879 836 787 597 393 268 217 41 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2099 Size: 5542 EcoO109I rG'GnC_Cy Cuts at: 2332 2347 2332 Size: 15 5527 EcoRI G'AATT_C Cuts at: 2851 3229 2851 Size: 378 5164 FspI TGC'GCA Cuts at: 4048 4048 Size: 5542 HaeII r_GCGC'y Cuts at: 1678 2068 4689 1678 Size: 390 2621 2531 Fragments arranged by size: 2621 2531 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2530 2562 4183 4215 4257 Size: 32 135 32 958 32 1621 32 42 Cuts at: 4257 4289 1373 Size: 32 2626 Fragments arranged by size: 2626 1621 958 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 2886 3132 669 Size: 988 281 543 405 246 3079 Fragments arranged by size: 3079 988 543 405 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5542 HpaI GTT'AAC Cuts at: 3132 3132 Size: 5542 KpnI G_GTAC'C Cuts at: 2867 2867 Size: 5542 MfeI C'AATT_G Cuts at: 3141 3141 Size: 5542 MluI A'CGCG_T Cuts at: 5418 5418 Size: 5542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 4656 4840 1305 Size: 1170 2181 184 2007 Fragments arranged by size: 2181 2007 1170 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3720 Fragments arranged by size: 3720 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3500 3859 4018 518 Size: 648 2334 359 159 2042 Fragments arranged by size: 2334 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2704 2958 3635 4783 5028 1606 Size: 921 177 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 254 245 177 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4990 NdeI CA'TA_TG Cuts at: 387 387 Size: 5542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5542 NotI GC'GGCC_GC Cuts at: 2989 2989 Size: 5542 NspI r_CATG'y Cuts at: 1132 1229 2900 4445 1132 Size: 97 1671 1545 2229 Fragments arranged by size: 2229 1671 1545 97 PasI CC'CwG_GG Cuts at: 1348 2336 2689 1348 Size: 988 353 4201 Fragments arranged by size: 4201 988 353 PciI A'CATG_T Cuts at: 4441 4441 Size: 5542 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5542 PmeI GTTT'AAAC Cuts at: 2846 2846 Size: 5542 PmlI CAC'GTG Cuts at: 2759 2759 Size: 5542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3611 3643 5124 5156 3611 Size: 32 1481 32 3997 Fragments arranged by size: 3997 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5542 PsiI TTA'TAA Cuts at: 3112 3112 Size: 5542 PstI C_TGCA'G Cuts at: 830 2436 2532 2894 830 Size: 1606 96 362 3478 Fragments arranged by size: 3478 1606 362 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5542 PvuII CAG'CTG Cuts at: 2527 2704 2527 Size: 177 5365 SacI G_AGCT'C Cuts at: 2036 2733 2036 Size: 697 4845 SalI G'TCGA_C Cuts at: 1936 2884 1936 Size: 948 4594 SbfI CC_TGCA'GG Cuts at: 2894 2894 Size: 5542 ScaI AGT'ACT Cuts at: 3790 5502 3790 Size: 1712 3830 SexAI A'CCwGG_T Cuts at: 2259 2304 2540 2259 Size: 45 236 5261 Fragments arranged by size: 5261 236 45 SfcI C'TryA_G Cuts at: 826 1045 2432 2528 2890 4025 4706 4897 826 Size: 219 1387 96 362 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 681 362 219 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5542 SmaI CCC'GGG Cuts at: 1872 2277 2869 1872 Size: 405 592 4545 Fragments arranged by size: 4545 592 405 SmlI C'TyrA_G Cuts at: 820 1017 1943 3646 4547 4809 820 Size: 197 926 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3333 493 Size: 2840 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5542 SphI G_CATG'C Cuts at: 1132 2900 1132 Size: 1768 3774 SspI AAT'ATT Cuts at: 5 52 3466 5 Size: 47 3414 2081 Fragments arranged by size: 3414 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5542 StyI C'CwwG_G Cuts at: 513 1065 2100 2981 513 Size: 552 1035 881 3074 Fragments arranged by size: 3074 1035 881 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 3569 3586 3739 3924 4369 325 Size: 1255 1037 952 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1037 952 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3788 5500 96 Size: 274 80 33 51 1443 1811 1712 138 Fragments arranged by size: 1811 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2748 2780 5502 5534 2748 Size: 32 2722 32 2756 Fragments arranged by size: 2756 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5542 XbaI T'CTAG_A Cuts at: 2878 2878 Size: 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5542 XmaI C'CCGG_G Cuts at: 1870 2275 2867 1870 Size: 405 592 4545 Fragments arranged by size: 4545 592 405 XmnI GAAnn'nnTTC Cuts at: 2456 3671 2456 Size: 1215 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3350 276 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI