Restriction map for
pFN21AB7570
(Linear) (Six-base) MAPSORT of: KIBB7570.seq Check: 6590 from: 1 to: 837
KIBB7570.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 516 837
Size: 516 321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 837
Size: 680 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 309 837
Size: 309 528
AlwNI CAG_nnn'CTG
Cuts at: 0 288 507 837
Size: 288 219 330
Fragments arranged by size:
330 288 219
ApoI r'AATT_y
Cuts at: 0 451 837
Size: 451 386
AvaI C'yCGr_G
Cuts at: 0 270 837
Size: 270 567
BanII G_rGCy'C
Cuts at: 0 31 190 707 728 837
Size: 31 159 517 21 109
Fragments arranged by size:
517 159 109 31 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 538 837
Size: 538 299
BbvCI CC'TCA_GC
Cuts at: 0 19 323 837
Size: 19 304 514
Fragments arranged by size:
514 304 19
BclI T'GATC_A
Cuts at: 0 224 837
Size: 224 613
BlpI GC'TnA_GC
Cuts at: 0 25 837
Size: 25 812
Bme1580I G_kGCm'C
Cuts at: 0 145 837
Size: 145 692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 333 837
Size: 108 225 504
Fragments arranged by size:
504 225 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 721 837
Size: 463 258 116
Fragments arranged by size:
463 258 116
Bpu10I CC'TnA_GC
Cuts at: 0 19 323 837
Size: 19 304 514
Fragments arranged by size:
514 304 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 321 837
Size: 94 227 516
Fragments arranged by size:
516 227 94
BsaAI yAC'GTr
Cuts at: 0 754 837
Size: 754 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 736 837
Size: 205 531 101
Fragments arranged by size:
531 205 101
BsiHKAI G_wGCw'C
Cuts at: 0 31 728 837
Size: 31 697 109
Fragments arranged by size:
697 109 31
BsmI GAATG_Cn'
Cuts at: 0 174 837
Size: 174 663
BsmBI CGTCTCn'nnnn_
Cuts at: 0 358 837
Size: 358 479
Bsp1286I G_dGCh'C
Cuts at: 0 31 145 190 707 728 837
Size: 31 114 45 517 21 109
Fragments arranged by size:
517 114 109 45 31 21
BspHI T'CATG_A
Cuts at: 0 74 837
Size: 74 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 516 837
Size: 112 404 321
Fragments arranged by size:
404 321 112
BsrBI CCG'CTC
Cuts at: 0 213 837
Size: 213 624
BstEII G'GTnAC_C
Cuts at: 0 303 837
Size: 303 534
BstYI r'GATC_y
Cuts at: 0 86 837
Size: 86 751
BtsI GCAGTG_nn'
Cuts at: 0 3 837
Size: 3 834
EaeI y'GGCC_r
Cuts at: 0 36 837
Size: 36 801
EarI CTCTTCn'nnn_
Cuts at: 0 641 656 819 837
Size: 641 15 163 18
Fragments arranged by size:
641 163 18 15
EcoICRI GAG'CTC
Cuts at: 0 29 726 837
Size: 29 697 111
Fragments arranged by size:
697 111 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 680 721 837
Size: 463 217 41 116
Fragments arranged by size:
463 217 116 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 837
Size: 94 743
EcoO109I rG'GnC_Cy
Cuts at: 0 327 342 837
Size: 327 15 495
Fragments arranged by size:
495 327 15
HaeII r_GCGC'y
Cuts at: 0 63 837
Size: 63 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 525 557 837
Size: 525 32 280
Fragments arranged by size:
525 280 32
HincII GTy'rAC
Cuts at: 0 476 837
Size: 476 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 837
Size: 470 367
MspA1I CmG'CkG
Cuts at: 0 522 699 837
Size: 522 177 138
Fragments arranged by size:
522 177 138
PasI CC'CwG_GG
Cuts at: 0 331 684 837
Size: 331 353 153
Fragments arranged by size:
353 331 153
PmlI CAC'GTG
Cuts at: 0 754 837
Size: 754 83
PstI C_TGCA'G
Cuts at: 0 431 527 837
Size: 431 96 310
Fragments arranged by size:
431 310 96
PvuII CAG'CTG
Cuts at: 0 522 699 837
Size: 522 177 138
Fragments arranged by size:
522 177 138
SacI G_AGCT'C
Cuts at: 0 31 728 837
Size: 31 697 109
Fragments arranged by size:
697 109 31
SexAI A'CCwGG_T
Cuts at: 0 254 299 535 837
Size: 254 45 236 302
Fragments arranged by size:
302 254 236 45
SfcI C'TryA_G
Cuts at: 0 427 523 837
Size: 427 96 314
Fragments arranged by size:
427 314 96
SmaI CCC'GGG
Cuts at: 0 272 837
Size: 272 565
StyI C'CwwG_G
Cuts at: 0 95 837
Size: 95 742
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 837
Size: 612 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 743 775 837
Size: 743 32 62
Fragments arranged by size:
743 62 32
XmaI C'CCGG_G
Cuts at: 0 270 837
Size: 270 567
XmnI GAAnn'nnTTC
Cuts at: 0 451 837
Size: 451 386
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BanII BbsI
BbvCI BclI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaAI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BstEII BstYI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MmeI MspA1I PasI
PmlI PstI PvuII SacI SexAI SfcI SmaI StyI
TaqII TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BglII BmgBI BmrI BmtI BpuEI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI ZraI