Restriction map for
pFN21AB5734
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsgI BsiWI
BsoBI BspHI BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CelII Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 2027 GACGTC GACGT^C
AccI 649 GTCTAC GT^[AC][GT]AC
AocI 1883 CCTTAGG CC^T[ACGT]AGG
ApaI 2163 GGGCCC GGGCC^C
AspI 1540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 383 GGATCC G^GATCC
BanI 905 GGTGCC G^G[CT][GA]CC
BbvCI 465 GCTGAGG CCTCAGC(-5/-2)
BcgI 729 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1742 AGATCT A^GATCT
BsaBI 864 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 846 CGACCG CG[GA][CT]^CG
BsmBI 1301 GAGACG CGTCTC(1/5)
BsmI 2019 GAATGC GAATGC(1/-1)
Bsp1407I 2119 TGTACA T^GTACA
BsrBI 2175 CCGCTC CCGCTC(-3/-3)
BsrGI 2119 TGTACA T^GTACA
BstBI 1675 TTCGAA TT^CGAA
Bsu36I 1883 CCTTAGG CC^T[ACGT]AGG
CfoI 840 GCGC GCG^C
DraI 1071 TTTAAA TTT^AAA
DraII 14 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 790 GACATTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 97 CTCTTC CTCTTC(1/4)
Eco81I 1883 CCTTAGG CC^T[ACGT]AGG
EcoO109I 14 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 346 GAATTC G^AATTC
EcoT22I 1813 ATGCAT ATGCA^T
HaeII 839 GGCGCT [GA]GCGC^[CT]
HgaI 2112 GACGC GACGC(5/10)
HhaI 840 GCGC GCG^C
HinP1I 840 GCGC G^CGC
Ksp632I 97 CTCTTC CTCTTC(1/4)
MaeII 2028 ACGT A^CGT
MamI 864 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1985 CAATTG C^AATTG
MunI 1985 CAATTG C^AATTG
NdeI 1486 CATATG CA^TATG
NsiI 1813 ATGCAT ATGCA^T
NspV 1675 TTCGAA TT^CGAA
PflFI 1540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1826 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 847 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 43 CAGCTG CAG^CTG
SapI 96 GCTCTTC GCTCTTC(1/4)
SfuI 1675 TTCGAA TT^CGAA
SphI 1811 GCATGC GCATG^C
SspBI 2119 TGTACA T^GTACA
StuI 1880 AGGCCT AGG^CCT
SwaI 1070 ATTTAAAT ATTT^AAAT
Tth111I 1540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1826 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1323 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 866 TCCGGA T^CCGGA
1632 TCCGGA
AcyI 2027 GACGTC G[GA]^CG[CT]C
2112 GACGCC
AflIII 617 ACATGT A^C[GA][CT]GT
807 ACATGT
AlwNI 691 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1438 CAGAATCTG
BalI 2 TGGCCA TGG^CCA
2036 TGGCCA
BanII 1249 GGGCTC G[GA]GC[CT]^C
2163 GGGCCC
BbsI 334 GAAGAC GAAGAC(2/6)
1056 GAAGAC
BpuAI 334 GAAGAC GAAGAC(2/6)
1056 GAAGAC
BsaHI 2027 GACGTC G[GA]^CG[CT]C
2112 GACGCC
BsaI 1327 GAGACC GGTCTC(1/5)
1848 GAGACC
BseAI 866 TCCGGA T^CCGGA
1632 TCCGGA
BspDI 1493 ATCGAT AT^CGAT
1604 ATCGAT
BspEI 866 TCCGGA T^CCGGA
1632 TCCGGA
BspLU11I 617 ACATGT A^CATGT
807 ACATGT
BstAPI 656 GCAATCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1170 GCAGAGGGTGC
BstXI 1007 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2153 CCATGCTCATGG
ClaI 1493 ATCGAT AT^CGAT
1604 ATCGAT
DsaI 1061 CCATGG C^C[GA][CT]GG
1854 CCATGG
EcoRV 1861 GATATC GAT^ATC
1918 GATATC
FauI 2174 CCCGC CCCGC(4/6)
1157 GCGGG
Hin1I 2027 GACGTC G[GA]^CG[CT]C
2112 GACGCC
MluNI 2 TGGCCA TGG^CCA
2036 TGGCCA
MroI 866 TCCGGA T^CCGGA
1632 TCCGGA
MscI 2 TGGCCA TGG^CCA
2036 TGGCCA
NcoI 1061 CCATGG C^CATGG
1854 CCATGG
SfcI 418 CTACAG C^T[GA][CT]AG
1892 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII