(Circular) (Six-base) MAPSORT of: pFN21AB5734.seq Check: 3405 from: 1 to: 6910 pFN21AB5734 6910 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4036 4720 278 Size: 53 83 186 936 2500 684 2468 Fragments arranged by size: 2500 2468 936 684 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 4253 1937 Size: 718 1598 4594 Fragments arranged by size: 4594 1598 718 Acc65I G'GTAC_C Cuts at: 4231 4231 Size: 6910 AclI AA'CG_TT Cuts at: 1563 5037 5410 1563 Size: 3474 373 3063 Fragments arranged by size: 3474 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2468 2624 3552 4973 6357 6625 1632 Size: 836 156 928 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 928 836 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6713 AflIII A'CryG_T Cuts at: 1203 2622 2812 5809 6786 1203 Size: 1419 190 2997 977 1327 Fragments arranged by size: 2997 1419 1327 977 190 AgeI A'CCGG_T Cuts at: 5741 5741 Size: 6910 AhdI GACnn_n'nnGTC Cuts at: 5639 5639 Size: 6910 AleI CACnn'nnGTG Cuts at: 1166 2564 2815 1166 Size: 1398 251 5261 Fragments arranged by size: 5261 1398 251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3815 1184 Size: 2631 4279 AlwNI CAG_nnn'CTG Cuts at: 2701 3448 4653 6225 2701 Size: 747 1205 1572 3386 Fragments arranged by size: 3386 1572 1205 747 ApaI G_GGCC'C Cuts at: 4172 4172 Size: 6910 ApaLI G'TGCA_C Cuts at: 4967 6123 4967 Size: 1156 5754 ApoI r'AATT_y Cuts at: 1493 2351 3079 3683 4219 4445 4597 6706 1493 Size: 858 728 604 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 858 728 604 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 5464 160 Size: 872 4432 1606 Fragments arranged by size: 4432 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 AvaI C'yCGr_G Cuts at: 1870 1943 4235 1870 Size: 73 2292 4545 Fragments arranged by size: 4545 2292 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3924 3957 3924 Size: 33 6877 BamHI G'GATC_C Cuts at: 2388 4240 4607 2388 Size: 1852 367 4691 Fragments arranged by size: 4691 1852 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2910 4231 5686 618 Size: 325 217 627 1123 1321 1455 1842 Fragments arranged by size: 1842 1455 1321 1123 627 325 217 BanII G_rGCy'C Cuts at: 1391 3258 4172 4229 1391 Size: 1867 914 57 4072 Fragments arranged by size: 4072 1867 914 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2346 3068 928 Size: 971 447 722 4770 Fragments arranged by size: 4770 971 722 447 BbvCI CC'TCA_GC Cuts at: 2471 2471 Size: 6910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2723 2757 5101 5135 1903 Size: 34 786 34 2344 34 3678 Fragments arranged by size: 3678 2344 786 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4802 6012 4802 Size: 1210 5700 BclI T'GATC_A Cuts at: 1548 1548 Size: 6910 BfrBI ATG'CAT Cuts at: 3820 3820 Size: 6910 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5521 136 Size: 107 122 71 631 605 3849 1525 Fragments arranged by size: 3849 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3747 6905 3747 Size: 3158 3752 BlpI GC'TnA_GC Cuts at: 4327 4327 Size: 6910 Bme1580I G_kGCm'C Cuts at: 1790 4172 4971 6127 1790 Size: 2382 799 1156 2573 Fragments arranged by size: 2573 2382 1156 799 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6910 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 3006 3542 Size: 416 53 83 399 24 611 978 536 Cuts at: 3542 4641 5599 442 Size: 1099 958 1753 Fragments arranged by size: 1753 1099 978 958 611 536 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6910 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 2612 3746 4001 5570 1239 Size: 1103 270 1134 255 1569 2579 Fragments arranged by size: 2579 1569 1134 1103 270 255 Bpu10I CC'TnA_GC Cuts at: 2213 2471 2213 Size: 258 6652 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 3446 4004 4225 5035 5900 6198 2517 Size: 929 558 221 810 865 298 3229 Fragments arranged by size: 3229 929 865 810 558 298 221 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3326 3847 5573 882 Size: 617 1827 521 1726 2219 Fragments arranged by size: 2219 1827 1726 617 521 BsaAI yAC'GTr Cuts at: 493 1204 4701 493 Size: 711 3497 2702 Fragments arranged by size: 3497 2702 711 BsaBI GATnn'nnATC Cuts at: 2873 6904 2873 Size: 4031 2879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4033 4118 4717 5099 Size: 53 83 186 936 2500 85 599 382 Cuts at: 5099 5734 275 Size: 635 1451 Fragments arranged by size: 2500 1451 936 635 599 382 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2871 3031 3637 5343 5741 6015 6162 6573 2871 Size: 160 606 1706 398 274 147 411 3208 Fragments arranged by size: 3208 1706 606 411 398 274 160 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6685 Fragments arranged by size: 6685 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2841 3441 3743 1186 Size: 1655 600 302 4353 Fragments arranged by size: 4353 1655 600 302 BseYI C'CCAG_C Cuts at: 2046 2552 2584 6113 6609 2046 Size: 506 32 3529 496 2347 Fragments arranged by size: 3529 2347 506 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 4360 5121 6149 1540 Size: 275 186 853 1506 761 1028 2301 Fragments arranged by size: 2301 1506 1028 853 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3089 3186 4052 4971 5056 6127 3089 Size: 97 866 919 85 1071 3872 Fragments arranged by size: 3872 1071 919 866 97 85 BsmI GAATG_Cn' Cuts at: 4030 4421 4514 4030 Size: 391 93 6426 Fragments arranged by size: 6426 391 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3300 3300 Size: 6910 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3089 3186 3258 4052 4172 4229 4971 Size: 399 1299 97 72 794 120 57 742 Cuts at: 4971 5056 6127 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1299 1071 794 742 399 120 97 85 72 57 BspEI T'CCGG_A Cuts at: 2871 3637 6573 2871 Size: 766 2936 3208 Fragments arranged by size: 3208 2936 766 BspHI T'CATG_A Cuts at: 4799 6529 4799 Size: 1730 5180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4265 844 Size: 3421 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4182 4797 1394 Size: 429 2359 615 3507 Fragments arranged by size: 3507 2359 615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5405 5579 65 Size: 1183 4157 174 1396 Fragments arranged by size: 4157 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5554 5741 1700 Size: 110 3744 187 2869 Fragments arranged by size: 3744 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4124 96 Size: 4028 2882 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6910 BssSI C'ACGA_G Cuts at: 4970 5982 4970 Size: 1012 5898 BstAPI GCAn_nnn'nTGC Cuts at: 794 2667 3181 794 Size: 1873 514 4523 Fragments arranged by size: 4523 1873 514 BstBI TT'CG_AA Cuts at: 3681 5714 3681 Size: 2033 4877 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6910 BstXI CCAn_nnnn'nTGG Cuts at: 3019 4165 3019 Size: 1146 5764 BstYI r'GATC_y Cuts at: 1972 2388 2868 2874 2922 3456 3747 3766 4240 Size: 416 480 6 48 534 291 19 474 Cuts at: 4240 4607 4992 5009 6461 6547 6559 6905 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 534 480 474 416 385 367 346 291 86 48 19 17 12 6 Bsu36I CC'TnA_GG Cuts at: 3889 3889 Size: 6910 BtgI C'CryG_G Cuts at: 513 1065 2004 3066 3859 513 Size: 552 939 1062 793 3564 Fragments arranged by size: 3564 1062 939 793 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3778 487 Size: 683 56 2552 3619 Fragments arranged by size: 3619 2552 683 56 BtsI GCAGTG_nn' Cuts at: 2248 2524 2570 3804 4426 5220 5240 2248 Size: 276 46 1234 622 794 20 3918 Fragments arranged by size: 3918 1234 794 622 276 46 20 ClaI AT'CG_AT Cuts at: 1554 3499 3610 1554 Size: 1945 111 4854 Fragments arranged by size: 4854 1945 111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2262 2297 2522 2557 529 Size: 35 1698 35 225 35 4882 Fragments arranged by size: 4882 1698 225 35 35 35 DraI TTT'AAA Cuts at: 3078 4214 3078 Size: 1136 5774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2801 5917 809 Size: 507 1485 3116 1802 Fragments arranged by size: 3116 1802 1485 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2333 4041 Size: 54 1425 50 275 18 177 326 1708 Cuts at: 4041 4357 5246 6875 8 Size: 316 889 1629 43 Fragments arranged by size: 1708 1629 1425 889 326 316 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4357 1537 Size: 275 2545 4090 Fragments arranged by size: 4090 2545 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 4840 1353 Size: 755 2732 3423 Fragments arranged by size: 3423 2732 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5497 5871 6017 387 Size: 1064 138 3908 374 146 1280 Fragments arranged by size: 3908 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2468 2612 2624 3552 3746 4001 Size: 393 710 126 144 12 928 194 255 Cuts at: 4001 4973 5570 6357 6625 1239 Size: 972 597 787 268 1524 Fragments arranged by size: 1524 972 928 787 710 597 393 268 255 194 144 126 12 EcoNI CCTnn'n_nnAGG Cuts at: 2321 3233 3249 2321 Size: 912 16 5982 Fragments arranged by size: 5982 912 16 EcoO109I rG'GnC_Cy Cuts at: 2020 2020 Size: 6910 EcoRI G'AATT_C Cuts at: 2351 4219 4597 2351 Size: 1868 378 4664 Fragments arranged by size: 4664 1868 378 EcoRV GAT'ATC Cuts at: 3868 3925 3868 Size: 57 6853 FspI TGC'GCA Cuts at: 5416 5416 Size: 6910 HaeII r_GCGC'y Cuts at: 1678 2848 6057 1678 Size: 1170 3209 2531 Fragments arranged by size: 3209 2531 1170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3488 3520 3603 3635 5551 Size: 32 135 32 1916 32 83 32 1916 Cuts at: 5551 5583 5625 5657 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1916 1916 135 83 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4254 4500 669 Size: 988 281 2316 246 3079 Fragments arranged by size: 3079 2316 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6910 HpaI GTT'AAC Cuts at: 4500 4500 Size: 6910 KpnI G_GTAC'C Cuts at: 4235 4235 Size: 6910 MfeI C'AATT_G Cuts at: 3990 4509 3990 Size: 519 6391 MluI A'CGCG_T Cuts at: 6786 6786 Size: 6910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3855 6024 6208 1305 Size: 2550 2169 184 2007 Fragments arranged by size: 2550 2169 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 4043 10 Size: 54 1425 343 177 2034 2877 Fragments arranged by size: 2877 2034 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 2815 2958 3797 4163 4868 5227 Size: 648 1398 251 143 839 366 705 359 Cuts at: 5227 5386 518 Size: 159 2042 Fragments arranged by size: 2042 1398 839 705 648 366 359 251 159 143 MspA1I CmG'CkG Cuts at: 1606 2050 2856 3763 4326 5003 6151 6396 1606 Size: 444 806 907 563 677 1148 245 2120 Fragments arranged by size: 2120 1148 907 806 677 563 444 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3066 3859 513 Size: 552 939 1062 793 3564 Fragments arranged by size: 3564 1062 939 793 552 NdeI CA'TA_TG Cuts at: 387 3492 387 Size: 3105 3805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6910 NotI GC'GGCC_GC Cuts at: 4357 4357 Size: 6910 NsiI A_TGCA'T Cuts at: 3822 3822 Size: 6910 NspI r_CATG'y Cuts at: 1132 1229 2626 2816 3717 3820 4268 5813 1132 Size: 97 1397 190 901 103 448 1545 2229 Fragments arranged by size: 2229 1545 1397 901 448 190 103 97 PasI CC'CwG_GG Cuts at: 1348 3385 1348 Size: 2037 4873 PciI A'CATG_T Cuts at: 2622 2812 5809 2622 Size: 190 2997 3723 Fragments arranged by size: 3723 2997 190 PflMI CCAn_nnn'nTGG Cuts at: 1108 3837 1108 Size: 2729 4181 PfoI T'CCnGG_A Cuts at: 2391 2976 2391 Size: 585 6325 PmeI GTTT'AAAC Cuts at: 4214 4214 Size: 6910 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4979 5011 6492 6524 4979 Size: 32 1481 32 5365 Fragments arranged by size: 5365 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2856 1613 Size: 1243 5667 PsiI TTA'TAA Cuts at: 4480 4480 Size: 6910 PspOMI G'GGCC_C Cuts at: 4168 4168 Size: 6910 PstI C_TGCA'G Cuts at: 830 4262 830 Size: 3432 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6910 PvuII CAG'CTG Cuts at: 2050 2050 Size: 6910 SalI G'TCGA_C Cuts at: 1936 4252 1936 Size: 2316 4594 SapI GCTCTTCn'nnn_ Cuts at: 2108 2108 Size: 6910 SbfI CC_TGCA'GG Cuts at: 4262 4262 Size: 6910 ScaI AGT'ACT Cuts at: 5158 6870 5158 Size: 1712 5198 SfcI C'TryA_G Cuts at: 826 1045 2423 3897 4258 5393 6074 6265 826 Size: 219 1378 1474 361 1135 681 191 1471 Fragments arranged by size: 1474 1471 1378 1135 681 361 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6910 SmaI CCC'GGG Cuts at: 1872 4237 1872 Size: 2365 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 3461 4019 4204 5014 5915 Size: 197 926 589 929 558 185 810 901 Cuts at: 5915 6177 820 Size: 262 1553 Fragments arranged by size: 1553 929 926 901 810 589 558 262 197 185 SnaBI TAC'GTA Cuts at: 493 4701 493 Size: 4208 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6910 SphI G_CATG'C Cuts at: 1132 3820 4268 1132 Size: 2688 448 3774 Fragments arranged by size: 3774 2688 448 SspI AAT'ATT Cuts at: 5 52 4834 5 Size: 47 4782 2081 Fragments arranged by size: 4782 2081 47 StuI AGG'CCT Cuts at: 1517 3887 1517 Size: 2370 4540 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2134 3066 3859 4349 513 Size: 552 939 6 124 932 793 490 3074 Fragments arranged by size: 3074 939 932 793 552 490 124 6 SwaI ATTT'AAAT Cuts at: 3078 3078 Size: 6910 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2809 3140 3144 4154 4937 4954 5107 Size: 1255 1229 331 4 1010 783 17 153 Cuts at: 5107 5292 5737 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1229 1010 783 445 331 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2721 4124 5156 Size: 274 80 33 51 1443 744 1403 1032 Cuts at: 5156 6868 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1403 1032 744 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6870 6902 6870 Size: 32 6878 Tth111I GACn'n_nGTC Cuts at: 1316 3548 1316 Size: 2232 4678 XbaI T'CTAG_A Cuts at: 3328 4246 3328 Size: 918 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6910 XmaI C'CCGG_G Cuts at: 1870 4235 1870 Size: 2365 4545 XmnI GAAnn'nnTTC Cuts at: 5039 5039 Size: 6910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4034 4718 276 Size: 53 83 186 936 2500 684 2468 Fragments arranged by size: 2500 2468 936 684 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI