Restriction map for
pFN21AB5734
(Linear) (Six-base) MAPSORT of: KIBB5734.seq Check: 5924 from: 1 to: 2205
KIBB5734.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 0 2031 2205
Size: 2031 174
AccI GT'mk_AC
Cuts at: 0 650 2205
Size: 650 1555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 619 1547 2205
Size: 463 156 928 658
Fragments arranged by size:
928 658 463 156
AflIII A'CryG_T
Cuts at: 0 617 807 2205
Size: 617 190 1398
Fragments arranged by size:
1398 617 190
AleI CACnn'nnGTG
Cuts at: 0 559 810 2205
Size: 559 251 1395
Fragments arranged by size:
1395 559 251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1810 2205
Size: 1810 395
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1443 2205
Size: 696 747 762
Fragments arranged by size:
762 747 696
ApaI G_GGCC'C
Cuts at: 0 2167 2205
Size: 2167 38
ApoI r'AATT_y
Cuts at: 0 346 1074 1678 2205
Size: 346 728 604 527
Fragments arranged by size:
728 604 527 346
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1919 1952 2205
Size: 1919 33 253
Fragments arranged by size:
1919 253 33
BamHI G'GATC_C
Cuts at: 0 383 2205
Size: 383 1822
BanI G'GyrC_C
Cuts at: 0 905 2205
Size: 905 1300
BanII G_rGCy'C
Cuts at: 0 1253 2167 2205
Size: 1253 914 38
Fragments arranged by size:
1253 914 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 341 1063 2205
Size: 341 722 1142
Fragments arranged by size:
1142 722 341
BbvCI CC'TCA_GC
Cuts at: 0 466 2205
Size: 466 1739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 752 2205
Size: 718 34 1453
Fragments arranged by size:
1453 718 34
BfrBI ATG'CAT
Cuts at: 0 1815 2205
Size: 1815 390
BglII A'GATC_T
Cuts at: 0 1742 2205
Size: 1742 463
Bme1580I G_kGCm'C
Cuts at: 0 2167 2205
Size: 2167 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 1001 1537 2205
Size: 23 978 536 668
Fragments arranged by size:
978 668 536 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 607 1741 1996 2205
Size: 337 270 1134 255 209
Fragments arranged by size:
1134 337 270 255 209
Bpu10I CC'TnA_GC
Cuts at: 0 208 466 2205
Size: 208 258 1739
Fragments arranged by size:
1739 258 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1441 1999 2205
Size: 512 929 558 206
Fragments arranged by size:
929 558 512 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 1321 1842 2205
Size: 1321 521 363
Fragments arranged by size:
1321 521 363
BsaBI GATnn'nnATC
Cuts at: 0 868 2205
Size: 868 1337
BsaHI Gr'CG_yC
Cuts at: 0 2028 2113 2205
Size: 2028 85 92
Fragments arranged by size:
2028 92 85
BsaWI w'CCGG_w
Cuts at: 0 866 1026 1632 2205
Size: 866 160 606 573
Fragments arranged by size:
866 606 573 160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 836 1436 1738 2205
Size: 836 600 302 467
Fragments arranged by size:
836 600 467 302
BseYI C'CCAG_C
Cuts at: 0 41 547 579 2205
Size: 41 506 32 1626
Fragments arranged by size:
1626 506 41 32
BsiEI CG_ry'CG
Cuts at: 0 849 2205
Size: 849 1356
BsiHKAI G_wGCw'C
Cuts at: 0 1084 1181 2047 2205
Size: 1084 97 866 158
Fragments arranged by size:
1084 866 158 97
BsmI GAATG_Cn'
Cuts at: 0 2025 2205
Size: 2025 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1295 2205
Size: 1295 910
Bsp1286I G_dGCh'C
Cuts at: 0 1084 1181 1253 2047 2167 2205
Size: 1084 97 72 794 120 38
Fragments arranged by size:
1084 794 120 97 72 38
BspEI T'CCGG_A
Cuts at: 0 866 1632 2205
Size: 866 766 573
Fragments arranged by size:
866 766 573
BsrBI CCG'CTC
Cuts at: 0 2177 2205
Size: 2177 28
BsrGI T'GTAC_A
Cuts at: 0 2119 2205
Size: 2119 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 662 1176 2205
Size: 662 514 1029
Fragments arranged by size:
1029 662 514
BstBI TT'CG_AA
Cuts at: 0 1676 2205
Size: 1676 529
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1014 2160 2205
Size: 1014 1146 45
Fragments arranged by size:
1146 1014 45
BstYI r'GATC_y
Cuts at: 0 383 863 869 917 1451 1742 1761 2205
Size: 383 480 6 48 534 291 19 444
Fragments arranged by size:
534 480 444 383 291 48 19 6
Bsu36I CC'TnA_GG
Cuts at: 0 1884 2205
Size: 1884 321
BtgI C'CryG_G
Cuts at: 0 1061 1854 2205
Size: 1061 793 351
Fragments arranged by size:
1061 793 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1773 2205
Size: 1773 432
BtsI GCAGTG_nn'
Cuts at: 0 243 519 565 1799 2205
Size: 243 276 46 1234 406
Fragments arranged by size:
1234 406 276 243 46
ClaI AT'CG_AT
Cuts at: 0 1494 1605 2205
Size: 1494 111 600
Fragments arranged by size:
1494 600 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 257 292 517 552 2205
Size: 257 35 225 35 1653
Fragments arranged by size:
1653 257 225 35 35
DraI TTT'AAA
Cuts at: 0 1073 2205
Size: 1073 1132
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 2205
Size: 796 1409
EaeI y'GGCC_r
Cuts at: 0 2 328 2036 2205
Size: 2 326 1708 169
Fragments arranged by size:
1708 326 169 2
EarI CTCTTCn'nnn_
Cuts at: 0 103 2205
Size: 103 2102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 463 607 619 1547 1741 1996 2205
Size: 337 126 144 12 928 194 255 209
Fragments arranged by size:
928 337 255 209 194 144 126 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 1228 1244 2205
Size: 316 912 16 961
Fragments arranged by size:
961 912 316 16
EcoO109I rG'GnC_Cy
Cuts at: 0 15 2205
Size: 15 2190
EcoRI G'AATT_C
Cuts at: 0 346 2205
Size: 346 1859
EcoRV GAT'ATC
Cuts at: 0 1863 1920 2205
Size: 1863 57 285
Fragments arranged by size:
1863 285 57
HaeII r_GCGC'y
Cuts at: 0 843 2205
Size: 843 1362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1483 1515 1598 1630 2205
Size: 1483 32 83 32 575
Fragments arranged by size:
1483 575 83 32 32
MfeI C'AATT_G
Cuts at: 0 1985 2205
Size: 1985 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 2205
Size: 1850 355
MscI TGG'CCA
Cuts at: 0 4 2038 2205
Size: 4 2034 167
Fragments arranged by size:
2034 167 4
MslI CAynn'nnrTG
Cuts at: 0 559 810 953 1792 2158 2205
Size: 559 251 143 839 366 47
Fragments arranged by size:
839 559 366 251 143 47
MspA1I CmG'CkG
Cuts at: 0 45 851 1758 2205
Size: 45 806 907 447
Fragments arranged by size:
907 806 447 45
NcoI C'CATG_G
Cuts at: 0 1061 1854 2205
Size: 1061 793 351
Fragments arranged by size:
1061 793 351
NdeI CA'TA_TG
Cuts at: 0 1487 2205
Size: 1487 718
NsiI A_TGCA'T
Cuts at: 0 1817 2205
Size: 1817 388
NspI r_CATG'y
Cuts at: 0 621 811 1712 1815 2205
Size: 621 190 901 103 390
Fragments arranged by size:
901 621 390 190 103
PasI CC'CwG_GG
Cuts at: 0 1380 2205
Size: 1380 825
PciI A'CATG_T
Cuts at: 0 617 807 2205
Size: 617 190 1398
Fragments arranged by size:
1398 617 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 1832 2205
Size: 1832 373
PfoI T'CCnGG_A
Cuts at: 0 386 971 2205
Size: 386 585 1234
Fragments arranged by size:
1234 585 386
PshAI GACnn'nnGTC
Cuts at: 0 851 2205
Size: 851 1354
PspOMI G'GGCC_C
Cuts at: 0 2163 2205
Size: 2163 42
PvuII CAG'CTG
Cuts at: 0 45 2205
Size: 45 2160
SapI GCTCTTCn'nnn_
Cuts at: 0 103 2205
Size: 103 2102
SfcI C'TryA_G
Cuts at: 0 418 1892 2205
Size: 418 1474 313
Fragments arranged by size:
1474 418 313
SmlI C'TyrA_G
Cuts at: 0 527 1456 2014 2199 2205
Size: 527 929 558 185 6
Fragments arranged by size:
929 558 527 185 6
SphI G_CATG'C
Cuts at: 0 1815 2205
Size: 1815 390
StuI AGG'CCT
Cuts at: 0 1882 2205
Size: 1882 323
StyI C'CwwG_G
Cuts at: 0 5 129 1061 1854 2205
Size: 5 124 932 793 351
Fragments arranged by size:
932 793 351 124 5
SwaI ATTT'AAAT
Cuts at: 0 1073 2205
Size: 1073 1132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 804 1135 1139 2149 2205
Size: 804 331 4 1010 56
Fragments arranged by size:
1010 804 331 56 4
TatI w'GTAC_w
Cuts at: 0 716 2119 2205
Size: 716 1403 86
Fragments arranged by size:
1403 716 86
Tth111I GACn'n_nGTC
Cuts at: 0 1543 2205
Size: 1543 662
XbaI T'CTAG_A
Cuts at: 0 1323 2205
Size: 1323 882
ZraI GAC'GTC
Cuts at: 0 2029 2205
Size: 2029 176
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApoI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BsrBI BsrGI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I MfeI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PshAI
PspOMI PvuII SapI SfcI SmlI SphI StuI StyI
SwaI TaqII TatI Tth111I XbaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BbeI BciVI
BclI BglI BlpI BmgBI BmtI BplI BsaAI BsaXI
BsgI BsiWI BspHI BspMI BsrDI BsrFI BssHII BssSI
BstEII BstZ17I DraIII EagI EciI EcoICRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI TstI XcmI XhoI XmaI XmnI