Restriction map for
pFN21AB8556
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaBI BsaI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1393 GACGTC GACGT^C
AccIII 1848 TCCGGA T^CCGGA
AhdI 1468 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 245 GTGCAC G^TGCAC
ApaI 950 GGGCCC GGGCC^C
ApaLI 245 GTGCAC G^TGCAC
AspEI 1468 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 531 GCACTTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 355 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 902 AGATCT A^GATCT
BsaAI 877 TACGTG [CT]AC^GT[GA]
BsaWI 1848 TCCGGA [AT]^CCGG[AT]
BseAI 1848 TCCGGA T^CCGGA
BsgI 1261 GTGCAG GTGCAG(16/14)
BsmBI 1755 CGTCTC CGTCTC(1/5)
BsmI 1553 GCATTC GAATGC(1/-1)
BspEI 1848 TCCGGA T^CCGGA
BspHI 461 TCATGA T^CATGA
BsrDI 2591 GCAATG GCAATG(2/0)
BstAPI 1836 GCAAGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2097 TTCGAA TT^CGAA
BstXI 560 CCATGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2515 GACCCATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1468 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1231 GAAGAG CTCTTC(1/4)
EcoNI 582 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1602 GTCAAC GT[CT]^[GA]AC
HindII 1602 GTCAAC GT[CT]^[GA]AC
Ksp632I 1231 GAAGAG CTCTTC(1/4)
MroI 1848 TCCGGA T^CCGGA
MslI 941 CACACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 945 CATATG CA^TATG
NspV 2097 TTCGAA TT^CGAA
PaeR7I 1312 CTCGAG C^TCGAG
PshAI 1162 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 461 TCATGA T^CATGA
SacI 1309 GAGCTC GAGCT^C
SexAI 1694 ACCAGGT A^CC[AT]GGT
SfuI 2097 TTCGAA TT^CGAA
SphI 290 GCATGC GCATG^C
SspI 1199 AATATT AAT^ATT
StuI 2552 AGGCCT AGG^CCT
XhoI 1312 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 573 GTAC GT^AC
2387 GTAC
AlwNI 495 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2602 CAGCAGCTG
AspI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACATGGTC
BamHI 1851 GGATCC G^GATCC
2067 GGATCC
BbeI 6 GGCGCC GGCGC^C
661 GGCGCC
BfaI 937 CTAG C^TAG
1282 CTAG
BlpI 1386 GCTGAGC GC^T[ACGT]AGC
2703 GCTCAGC
Bpu1102I 1386 GCTGAGC GC^T[ACGT]AGC
2703 GCTCAGC
BspMI 2349 ACCTGC ACCTGC(4/8)
72 GCAGGT
BsrBI 10 CCGCTC CCGCTC(-3/-3)
708 CCGCTC
CelII 1386 GCTGAGC GC^T[ACGT]AGC
2703 GCTCAGC
DraI 1891 TTTAAA TTT^AAA
2308 TTTAAA
DraIII 248 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
565 CACTTGGTG
FauI 1077 CCCGC CCCGC(4/6)
2622 GCGGG
HaeII 6 GGCGCC [GA]GCGC^[CT]
661 GGCGCC
HgaI 731 GCGTC GACGC(5/10)
1754 GCGTC
KasI 6 GGCGCC G^GCGCC
661 GGCGCC
MaeI 937 CTAG C^TAG
1282 CTAG
MaeII 878 ACGT A^CGT
1394 ACGT
NarI 6 GGCGCC GG^CGCC
661 GGCGCC
NcoI 1713 CCATGG C^CATGG
1787 CCATGG
PflFI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACATGGTC
PflMI 2002 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2251 CCACCTTCTGG
PleI 1419 GACTC GAGTC(4/5)
1568 GAGTC
PstI 347 CTGCAG CTGCA^G
2534 CTGCAG
RsaI 573 GTAC GT^AC
2387 GTAC
SfcI 347 CTGCAG C^T[GA][CT]AG
2534 CTGCAG
SfoI 6 GGCGCC GGC^GCC
661 GGCGCC
Tth111I 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1069 GACATGGTC
Van91I 2002 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2251 CCACCTTCTGG
XcmI 793 CCAACAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1695 CCAGGTGCTCGATGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII