(Circular) (Six-base) MAPSORT of: pFN21AB8556.seq Check: 3568 from: 1 to: 7435 pFN21AB8556 7435 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3402 5245 278 Size: 53 83 186 936 1866 1843 2468 Fragments arranged by size: 2468 1866 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4778 1937 Size: 2841 4594 Acc65I G'GTAC_C Cuts at: 4756 4756 Size: 7435 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 3623 4219 4239 5498 6882 7150 1632 Size: 1244 747 596 20 1259 1384 268 1917 Fragments arranged by size: 1917 1384 1259 1244 747 596 268 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7238 AflIII A'CryG_T Cuts at: 1203 6334 7311 1203 Size: 5131 977 1327 Fragments arranged by size: 5131 1327 977 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 3478 6164 3478 Size: 2686 4749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7435 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4171 4203 4171 Size: 32 7403 AlwNI CAG_nnn'CTG Cuts at: 2505 4612 5178 6750 2505 Size: 2107 566 1572 3190 Fragments arranged by size: 3190 2107 1572 566 ApaI G_GGCC'C Cuts at: 2959 2959 Size: 7435 ApaLI G'TGCA_C Cuts at: 2250 5492 6648 2250 Size: 3242 1156 3037 Fragments arranged by size: 3242 3037 1156 ApoI r'AATT_y Cuts at: 1493 2050 2287 2309 2393 4744 4970 5122 7231 Size: 557 237 22 84 2351 226 152 2109 Cuts at: 7231 1493 Size: 1697 Fragments arranged by size: 2351 2109 1697 557 237 226 152 84 22 AseI AT'TA_AT Cuts at: 160 1032 5989 160 Size: 872 4957 1606 Fragments arranged by size: 4957 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 2920 3317 4427 4454 4567 4760 1870 Size: 73 977 397 1110 27 113 193 4545 Fragments arranged by size: 4545 1110 977 397 193 113 73 27 BamHI G'GATC_C Cuts at: 3856 4072 4765 5132 3856 Size: 216 693 367 6159 Fragments arranged by size: 6159 693 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2666 2815 3722 4756 Size: 325 217 627 224 655 149 907 1034 Cuts at: 4756 6211 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1034 907 655 627 325 224 217 149 BanII G_rGCy'C Cuts at: 1391 2587 2601 2959 3318 3718 4754 1391 Size: 1196 14 358 359 400 1036 4072 Fragments arranged by size: 4072 1196 1036 400 359 358 14 BbeI G_GCGC'C Cuts at: 2015 2670 2015 Size: 655 6780 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 2864 3495 3879 928 Size: 971 758 207 631 384 4484 Fragments arranged by size: 4484 971 758 631 384 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2525 2559 5626 5660 1903 Size: 34 588 34 3067 34 3678 Fragments arranged by size: 3678 3067 588 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5327 6537 5327 Size: 1210 6225 BclI T'GATC_A Cuts at: 1548 1548 Size: 7435 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2366 6046 Size: 107 122 71 631 605 337 357 3680 Cuts at: 6046 136 Size: 1525 Fragments arranged by size: 3680 1525 631 605 357 337 122 107 71 BglII A'GATC_T Cuts at: 2907 7430 2907 Size: 4523 2912 BlpI GC'TnA_GC Cuts at: 3392 4709 4852 3392 Size: 1317 143 5975 Fragments arranged by size: 5975 1317 143 Bme1580I G_kGCm'C Cuts at: 1790 2254 2538 2607 2820 2959 5496 6652 1790 Size: 464 284 69 213 139 2537 1156 2573 Fragments arranged by size: 2573 2537 1156 464 284 213 139 69 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 2818 3181 Size: 416 53 83 399 24 957 444 363 Cuts at: 3181 5166 6124 442 Size: 1985 958 1753 Fragments arranged by size: 1985 1753 958 957 444 416 399 363 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2463 2609 3569 3707 4129 6095 1239 Size: 1224 146 960 138 422 1966 2579 Fragments arranged by size: 2579 1966 1224 960 422 146 138 Bpu10I CC'TnA_GC Cuts at: 3749 3749 Size: 7435 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 2768 3042 3324 5560 6425 6723 2296 Size: 472 274 282 2236 865 298 3008 Fragments arranged by size: 3008 2236 865 472 298 282 274 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6098 882 Size: 617 4599 2219 Fragments arranged by size: 4599 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2884 5226 493 Size: 711 1680 2342 2702 Fragments arranged by size: 2702 2342 1680 711 BsaBI GATnn'nnATC Cuts at: 7429 7429 Size: 7435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2667 3399 3759 Size: 53 83 186 936 479 655 732 360 Cuts at: 3759 5242 5624 6259 275 Size: 1483 382 635 1451 Fragments arranged by size: 1483 1451 936 732 655 635 479 382 360 186 83 53 BsaWI w'CCGG_w Cuts at: 3853 5868 6266 6540 6687 7098 3853 Size: 2015 398 274 147 411 4190 Fragments arranged by size: 4190 2015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2436 2466 1178 Size: 30 165 30 1033 30 6147 Fragments arranged by size: 6147 1033 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3045 4144 4267 4350 4525 4616 1186 Size: 1859 1099 123 83 175 91 4005 Fragments arranged by size: 4005 1859 1099 175 123 91 83 BseYI C'CCAG_C Cuts at: 2031 3543 3566 3648 4379 6638 7134 2031 Size: 1512 23 82 731 2259 496 2332 Fragments arranged by size: 2332 2259 1512 731 496 82 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 1163 Size: 573 1551 5311 Fragments arranged by size: 5311 1551 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2254 3318 3708 5496 5581 6652 2254 Size: 1064 390 1788 85 1071 3037 Fragments arranged by size: 3037 1788 1071 1064 390 85 BsmI GAATG_Cn' Cuts at: 3558 4946 5039 3558 Size: 1388 93 5954 Fragments arranged by size: 5954 1388 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3766 3766 Size: 7435 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2538 2587 2601 2607 2820 2959 Size: 399 464 284 49 14 6 213 139 Cuts at: 2959 3318 3708 3718 4754 5496 5581 6652 1391 Size: 359 390 10 1036 742 85 1071 2174 Fragments arranged by size: 2174 1071 1036 742 464 399 390 359 284 213 139 85 49 14 10 6 BspEI T'CCGG_A Cuts at: 3853 7098 3853 Size: 3245 4190 BspHI T'CATG_A Cuts at: 2466 5324 7054 2466 Size: 2858 1730 2847 Fragments arranged by size: 2858 2847 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 4363 4790 844 Size: 1224 2295 427 3489 Fragments arranged by size: 3489 2295 1224 427 BsrBI CCG'CTC Cuts at: 1394 1823 2017 2715 5322 1394 Size: 429 194 698 2607 3507 Fragments arranged by size: 3507 2607 698 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 4603 5930 6104 65 Size: 1183 3355 1327 174 1396 Fragments arranged by size: 3355 1396 1327 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6079 6266 1700 Size: 110 4269 187 2869 Fragments arranged by size: 4269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 5495 6507 5495 Size: 1012 6423 BstAPI GCAn_nnn'nTGC Cuts at: 794 3847 794 Size: 3053 4382 BstBI TT'CG_AA Cuts at: 4103 6239 4103 Size: 2136 5299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7435 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 7435 BstYI r'GATC_y Cuts at: 1972 2907 3856 3970 4072 4168 4186 4765 5132 Size: 935 949 114 102 96 18 579 367 Cuts at: 5132 5517 5534 6986 7072 7084 7430 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 949 935 579 385 367 346 114 102 96 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2728 2820 3718 3792 4012 4364 Size: 552 939 724 92 898 74 220 352 Cuts at: 4364 513 Size: 3584 Fragments arranged by size: 3584 939 898 724 552 352 220 92 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4687 487 Size: 683 56 3461 3235 Fragments arranged by size: 3461 3235 683 56 BtsI GCAGTG_nn' Cuts at: 4951 5745 5765 4951 Size: 794 20 6621 Fragments arranged by size: 6621 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7400 DraI TTT'AAA Cuts at: 3898 4315 4739 3898 Size: 417 424 6594 Fragments arranged by size: 6594 424 417 DraIII CAC_nnn'GTG Cuts at: 2258 2575 2258 Size: 317 7118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4526 6442 809 Size: 507 3210 1916 1802 Fragments arranged by size: 3210 1916 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2260 2562 Size: 54 1425 50 275 18 351 79 302 Cuts at: 2562 2890 2961 3741 4698 4882 5771 7400 8 Size: 328 71 780 957 184 889 1629 43 Fragments arranged by size: 1629 1425 957 889 780 351 328 302 275 184 79 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3231 5365 1353 Size: 1878 2134 3423 Fragments arranged by size: 3423 2134 1878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2599 4658 6022 6396 6542 387 Size: 1064 138 1010 2059 1364 374 146 1280 Fragments arranged by size: 2059 1364 1280 1064 1010 374 146 138 EcoICRI GAG'CTC Cuts at: 3316 3316 Size: 7435 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2609 2876 3569 3623 3707 4129 Size: 393 831 146 267 693 54 84 422 Cuts at: 4129 4219 4239 5498 6095 6882 7150 1239 Size: 90 20 1259 597 787 268 1524 Fragments arranged by size: 1524 1259 831 787 693 597 422 393 268 267 146 90 84 54 20 EcoNI CCTnn'n_nnAGG Cuts at: 2591 2591 Size: 7435 EcoO109I rG'GnC_Cy Cuts at: 2509 2956 3985 4203 4432 4501 4687 2509 Size: 447 1029 218 229 69 186 5257 Fragments arranged by size: 5257 1029 447 229 218 186 69 EcoRI G'AATT_C Cuts at: 4744 5122 4744 Size: 378 7057 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2141 2926 2141 Size: 785 6650 FspI TGC'GCA Cuts at: 5941 5941 Size: 7435 HaeII r_GCGC'y Cuts at: 1678 2015 2670 6582 1678 Size: 337 655 3912 2531 Fragments arranged by size: 3912 2531 655 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2436 2468 3464 3496 4571 Size: 32 135 32 864 32 996 32 1075 Cuts at: 4571 4603 6076 6108 6150 6182 1373 Size: 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 1075 996 864 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 4779 5025 669 Size: 988 281 1671 1170 246 3079 Fragments arranged by size: 3079 1671 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7435 HpaI GTT'AAC Cuts at: 5025 5025 Size: 7435 KasI G'GCGC_C Cuts at: 2011 2666 2011 Size: 655 6780 KpnI G_GTAC'C Cuts at: 4760 4760 Size: 7435 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3492 6549 6733 1305 Size: 2187 3057 184 2007 Fragments arranged by size: 3057 2187 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 2564 2892 3743 4700 Size: 54 1425 343 430 302 328 851 957 Cuts at: 4700 10 Size: 2745 Fragments arranged by size: 2745 1425 957 851 430 343 328 302 54 MslI CAynn'nnrTG Cuts at: 518 1166 2950 5393 5752 5911 518 Size: 648 1784 2443 359 159 2042 Fragments arranged by size: 2443 2042 1784 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3547 3580 3652 4383 4467 4612 4851 5528 Size: 1941 33 72 731 84 145 239 677 Cuts at: 5528 6676 6921 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1941 1148 731 677 245 239 145 84 72 33 NarI GG'CG_CC Cuts at: 2012 2667 2012 Size: 655 6780 NcoI C'CATG_G Cuts at: 513 1065 2004 3718 3792 513 Size: 552 939 1714 74 4156 Fragments arranged by size: 4156 1714 939 552 74 NdeI CA'TA_TG Cuts at: 387 2951 387 Size: 2564 4871 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7435 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NspI r_CATG'y Cuts at: 1132 1229 2299 3391 4726 4793 6338 1132 Size: 97 1070 1092 1335 67 1545 2229 Fragments arranged by size: 2229 1545 1335 1092 1070 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7435 PciI A'CATG_T Cuts at: 6334 6334 Size: 7435 PflMI CCAn_nnn'nTGG Cuts at: 1108 4013 4262 1108 Size: 2905 249 4281 Fragments arranged by size: 4281 2905 249 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5504 5536 7017 7049 5504 Size: 32 1481 32 5890 Fragments arranged by size: 5890 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 3985 4203 4501 4687 2509 Size: 1476 218 298 186 5257 Fragments arranged by size: 5257 1476 298 218 186 PshAI GACnn'nnGTC Cuts at: 1613 3171 1613 Size: 1558 5877 PsiI TTA'TAA Cuts at: 5005 5005 Size: 7435 PspOMI G'GGCC_C Cuts at: 2955 2955 Size: 7435 PspXI vC'TCGA_Gb Cuts at: 3317 3317 Size: 7435 PstI C_TGCA'G Cuts at: 830 2356 4543 4787 830 Size: 1526 2187 244 3478 Fragments arranged by size: 3478 2187 1526 244 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 PvuII CAG'CTG Cuts at: 3547 3580 3652 4612 3547 Size: 33 72 960 6370 Fragments arranged by size: 6370 960 72 33 SacI G_AGCT'C Cuts at: 3318 3318 Size: 7435 SalI G'TCGA_C Cuts at: 1936 4777 1936 Size: 2841 4594 SanDI GG'GwC_CC Cuts at: 3985 4501 3985 Size: 516 6919 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 5683 7395 5683 Size: 1712 5723 SexAI A'CCwGG_T Cuts at: 3699 3699 Size: 7435 SfcI C'TryA_G Cuts at: 826 1045 2352 4539 4783 5918 6599 6790 826 Size: 219 1307 2187 244 1135 681 191 1471 Fragments arranged by size: 2187 1471 1307 1135 681 244 219 191 SfoI GGC'GCC Cuts at: 2013 2668 2013 Size: 655 6780 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 4762 1872 Size: 2890 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 2783 3057 3317 3339 5539 Size: 197 926 332 508 274 260 22 2200 Cuts at: 5539 6440 6702 820 Size: 901 262 1553 Fragments arranged by size: 2200 1553 926 901 508 332 274 262 260 197 22 SnaBI TAC'GTA Cuts at: 493 5226 493 Size: 4733 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7435 SphI G_CATG'C Cuts at: 1132 2299 4793 1132 Size: 1167 2494 3774 Fragments arranged by size: 3774 2494 1167 SspI AAT'ATT Cuts at: 5 52 3206 5359 5 Size: 47 3154 2153 2081 Fragments arranged by size: 3154 2153 2081 47 StuI AGG'CCT Cuts at: 1517 4559 1517 Size: 3042 4393 StyI C'CwwG_G Cuts at: 513 1065 2004 2161 3718 3792 4281 4874 513 Size: 552 939 157 1557 74 489 593 3074 Fragments arranged by size: 3074 1557 939 593 552 489 157 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2800 2876 2945 3749 5462 5479 5632 Size: 1255 1220 76 69 804 1713 17 153 Cuts at: 5632 5817 6262 325 Size: 185 445 1498 Fragments arranged by size: 1713 1498 1255 1220 804 445 185 153 76 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4391 5681 7393 Size: 274 80 33 51 1443 2414 1290 1712 Cuts at: 7393 96 Size: 138 Fragments arranged by size: 2414 1712 1443 1290 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7395 7427 7395 Size: 32 7403 Tth111I GACn'n_nGTC Cuts at: 1316 2450 3077 1316 Size: 1134 627 5674 Fragments arranged by size: 5674 1134 627 XbaI T'CTAG_A Cuts at: 4771 4771 Size: 7435 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2805 3707 2805 Size: 902 6533 XhoI C'TCGA_G Cuts at: 1943 3317 1943 Size: 1374 6061 XmaI C'CCGG_G Cuts at: 1870 4760 1870 Size: 2890 4545 XmnI GAAnn'nnTTC Cuts at: 5564 5564 Size: 7435 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3400 5243 276 Size: 53 83 186 936 1866 1843 2468 Fragments arranged by size: 2468 1866 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SapI SfiI SrfI SwaI