Restriction map for
pFN21AB8521
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BglII
BlnI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MaeI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 1141 CTTAAG C^TTAAG
AflIII 155 ACATGT A^C[GA][CT]GT
Alw44I 2458 GTGCAC G^TGCAC
AocI 1551 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2027 AGCGCT AGC^GCT
ApaLI 2458 GTGCAC G^TGCAC
AviII 2086 TGCGCA TGC^GCA
BfrI 1141 CTTAAG C^TTAAG
BlpI 2295 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2295 GCTCAGC GC^T[ACGT]AGC
BsmBI 2192 GAGACG CGTCTC(1/5)
BsmI 1584 GAATGC GAATGC(1/-1)
BspHI 2645 TCATGA T^CATGA
BspLU11I 155 ACATGT A^CATGT
BsrDI 2234 GCAATG GCAATG(2/0)
BssHII 1745 GCGCGC G^CGCGC
BssSI 1335 CACGAG CACGAG(-5/-1)
BstAPI 2624 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2201 AGATCC [GA]^GATC[CT]
Bsu36I 1551 CCTGAGG CC^T[ACGT]AGG
CelII 2295 GCTCAGC GC^T[ACGT]AGC
CpoI 1450 CGGACCG CG^G[AT]CCG
DraIII 2855 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2027 AGCGCT AGC^GCT
Eco81I 1551 CCTGAGG CC^T[ACGT]AGG
FspI 2086 TGCGCA TGC^GCA
HincII 274 GTCAAC GT[CT]^[GA]AC
HindII 274 GTCAAC GT[CT]^[GA]AC
HphI 2636 TCACC GGTGA(8/7)
KspI 2317 CCGCGG CCGC^GG
MflI 2201 AGATCC [GA]^GATC[CT]
PflMI 1994 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 285 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2645 TCATGA T^CATGA
RsrII 1450 CGGACCG CG^G[AT]CCG
SacI 861 GAGCTC GAGCT^C
SacII 2317 CCGCGG CCGC^GG
SfiI 1089 GGCCAAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I 1994 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2775 CCAAGCCCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2201 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 1714 GGTACC G^GTACC
1916 GGTACC
AccIII 1558 TCCGGA T^CCGGA
2797 TCCGGA
AcsI 408 AAATTC [GA]^AATT[CT]
970 AAATTC
AlwI 2202 GATCC GGATC(4/5)
465 GGATC
ApaI 1760 GGGCCC GGGCC^C
2578 GGGCCC
ApoI 408 AAATTC [GA]^AATT[CT]
970 AAATTC
Asp718I 1714 GGTACC G^GTACC
1916 GGTACC
BalI 330 TGGCCA TGG^CCA
444 TGGCCA
BbeI 24 GGCGCC GGCGC^C
31 GGCGCC
BsaAI 1049 TACGTG [CT]AC^GT[GA]
2455 TACGTG
BsaWI 1558 TCCGGA [AT]^CCGG[AT]
2797 TCCGGA
BseAI 1558 TCCGGA T^CCGGA
2797 TCCGGA
BspEI 1558 TCCGGA T^CCGGA
2797 TCCGGA
BstXI 47 CCACAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAAGACCCTGG
EcoNI 1920 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2013 CCTGGCTAAGG
KasI 24 GGCGCC G^GCGCC
31 GGCGCC
KpnI 1714 GGTACC GGTAC^C
1916 GGTACC
MluNI 330 TGGCCA TGG^CCA
444 TGGCCA
MroI 1558 TCCGGA T^CCGGA
2797 TCCGGA
MscI 330 TGGCCA TGG^CCA
444 TGGCCA
MslI 48 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2230 CACGGCAATG
NarI 24 GGCGCC GG^CGCC
31 GGCGCC
NcoI 53 CCATGG C^CATGG
1911 CCATGG
NspI 155 ACATGT [GA]CATG^[CT]
2627 GCATGT
PvuII 1480 CAGCTG CAG^CTG
2212 CAGCTG
SfoI 24 GGCGCC GGC^GCC
31 GGCGCC
StuI 206 AGGCCT AGG^CCT
2171 AGGCCT
TaqI 317 TCGA T^CGA
367 TCGA
Tsp45I 1939 GTGAC ^GT[GC]AC
2524 GTGAC
TthHB8I 317 TCGA T^CGA
367 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI