(Circular) (Six-base) MAPSORT of: pFN21AB5716.seq Check: 3757 from: 1 to: 6868 pFN21AB5716 6868 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2428 2428 Size: 6868 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4678 278 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3853 4211 1937 Size: 1916 358 4594 Fragments arranged by size: 4594 1916 358 Acc65I G'GTAC_C Cuts at: 4189 4189 Size: 6868 AclI AA'CG_TT Cuts at: 1563 4995 5368 1563 Size: 3432 373 3063 Fragments arranged by size: 3432 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 2561 3017 3404 3429 4931 6315 6583 Size: 650 279 456 387 25 1502 1384 268 Cuts at: 6583 1632 Size: 1917 Fragments arranged by size: 1917 1502 1384 650 456 387 279 268 25 AfeI AGC'GCT Cuts at: 2030 2030 Size: 6868 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6671 AflIII A'CryG_T Cuts at: 1203 4066 4107 5767 6744 1203 Size: 2863 41 1660 977 1327 Fragments arranged by size: 2863 1660 1327 977 41 AgeI A'CCGG_T Cuts at: 2987 5699 2987 Size: 2712 4156 AhdI GACnn_n'nnGTC Cuts at: 3510 5597 3510 Size: 2087 4781 AleI CACnn'nnGTG Cuts at: 1166 3147 1166 Size: 1981 4887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2945 3165 1184 Size: 1761 220 4887 Fragments arranged by size: 4887 1761 220 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 2519 Size: 32 6836 AlwNI CAG_nnn'CTG Cuts at: 3063 3139 4611 6183 3063 Size: 76 1472 1572 3748 Fragments arranged by size: 3748 1572 1472 76 ApaLI G'TGCA_C Cuts at: 2720 4925 6081 2720 Size: 2205 1156 3507 Fragments arranged by size: 3507 2205 1156 ApoI r'AATT_y Cuts at: 1493 4177 4403 4555 6664 1493 Size: 2684 226 152 2109 1697 Fragments arranged by size: 2684 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5422 160 Size: 872 4390 1606 Fragments arranged by size: 4390 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 AvaI C'yCGr_G Cuts at: 1870 1943 3500 3764 4193 1870 Size: 73 1557 264 429 4545 Fragments arranged by size: 4545 1557 429 264 73 BamHI G'GATC_C Cuts at: 4198 4565 4198 Size: 367 6501 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2741 2984 3190 4189 Size: 325 217 627 394 560 243 206 999 Cuts at: 4189 5644 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 999 627 560 394 325 243 217 206 BanII G_rGCy'C Cuts at: 1391 3984 4077 4187 1391 Size: 2593 93 110 4072 Fragments arranged by size: 4072 2593 110 93 BbeI G_GCGC'C Cuts at: 2185 2185 Size: 6868 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2990 3843 928 Size: 971 1091 853 3953 Fragments arranged by size: 3953 1091 971 853 BbvCI CC'TCA_GC Cuts at: 2171 2666 2974 2171 Size: 495 308 6065 Fragments arranged by size: 6065 495 308 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5059 5093 1903 Size: 34 3122 34 3678 Fragments arranged by size: 3678 3122 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3414 4760 5970 3414 Size: 1346 1210 4312 Fragments arranged by size: 4312 1346 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6868 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3054 5479 136 Size: 107 122 71 631 605 1382 2425 1525 Fragments arranged by size: 2425 1525 1382 631 605 122 107 71 BglII A'GATC_T Cuts at: 3570 6863 3570 Size: 3293 3575 BlpI GC'TnA_GC Cuts at: 2553 2681 4285 2553 Size: 128 1604 5136 Fragments arranged by size: 5136 1604 128 Bme1580I G_kGCm'C Cuts at: 1790 2724 2844 2987 4929 6085 1790 Size: 934 120 143 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 934 143 120 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6868 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3489 4599 Size: 416 53 83 399 24 969 1103 1110 Cuts at: 4599 5557 442 Size: 958 1753 Fragments arranged by size: 1753 1110 1103 969 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2310 3326 3458 3694 3908 4061 5528 Size: 981 90 1016 132 236 214 153 1467 Cuts at: 5528 1239 Size: 2579 Fragments arranged by size: 2579 1467 1016 981 236 214 153 132 90 Bpu10I CC'TnA_GC Cuts at: 2171 2666 2974 3818 3831 2171 Size: 495 308 844 13 5208 Fragments arranged by size: 5208 844 495 308 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 4993 5858 6156 2744 Size: 2249 865 298 3456 Fragments arranged by size: 3456 2249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2467 2591 3420 5531 882 Size: 617 968 124 829 2111 2219 Fragments arranged by size: 2219 2111 968 829 617 124 BsaAI yAC'GTr Cuts at: 493 1204 2725 4659 493 Size: 711 1521 1934 2702 Fragments arranged by size: 2702 1934 1521 711 BsaBI GATnn'nnATC Cuts at: 3965 6862 3965 Size: 2897 3971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 2995 4675 5057 Size: 53 83 186 936 649 813 1680 382 Cuts at: 5057 5692 275 Size: 635 1451 Fragments arranged by size: 1680 1451 936 813 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2987 5301 5699 5973 6120 6531 2987 Size: 2314 398 274 147 411 3324 Fragments arranged by size: 3324 2314 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3153 3183 1178 Size: 30 165 30 1152 30 568 30 4863 Fragments arranged by size: 4863 1152 568 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2209 2393 2471 3038 4012 1186 Size: 979 44 184 78 567 974 4042 Fragments arranged by size: 4042 979 974 567 184 78 44 BseYI C'CCAG_C Cuts at: 3220 3232 3539 6071 6567 3220 Size: 12 307 2532 496 3521 Fragments arranged by size: 3521 2532 496 307 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2265 2388 3125 3635 3671 1163 Size: 573 529 123 737 510 36 4360 Fragments arranged by size: 4360 737 573 529 510 123 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2869 4318 5079 6107 1540 Size: 275 186 868 1449 761 1028 2301 Fragments arranged by size: 2301 1449 1028 868 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2724 3154 4929 5014 6085 2157 Size: 567 430 1775 85 1071 2940 Fragments arranged by size: 2940 1775 1071 567 430 85 BsmI GAATG_Cn' Cuts at: 4379 4472 4379 Size: 93 6775 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2724 2844 2987 3154 3984 4077 Size: 399 367 567 120 143 167 830 93 Cuts at: 4077 4187 4929 5014 6085 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 830 742 567 399 367 167 143 120 110 93 85 BspEI T'CCGG_A Cuts at: 6531 6531 Size: 6868 BspHI T'CATG_A Cuts at: 4757 6487 4757 Size: 1730 5138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2428 2449 2686 3531 4223 844 Size: 1584 21 237 845 692 3489 Fragments arranged by size: 3489 1584 845 692 237 21 BsrBI CCG'CTC Cuts at: 1394 1823 4755 1394 Size: 429 2932 3507 Fragments arranged by size: 3507 2932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3840 5363 5537 65 Size: 1183 2592 1523 174 1396 Fragments arranged by size: 2592 1523 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2716 2864 2987 3193 5512 5699 1700 Size: 110 906 148 123 206 2319 187 2869 Fragments arranged by size: 2869 2319 906 206 187 148 123 110 BsrGI T'GTAC_A Cuts at: 96 3640 96 Size: 3544 3324 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6868 BssSI C'ACGA_G Cuts at: 4928 5940 4928 Size: 1012 5856 BstAPI GCAn_nnn'nTGC Cuts at: 794 3139 794 Size: 2345 4523 BstBI TT'CG_AA Cuts at: 5672 5672 Size: 6868 BstEII G'GTnAC_C Cuts at: 1184 2959 1184 Size: 1775 5093 BstXI CCAn_nnnn'nTGG Cuts at: 3361 3361 Size: 6868 BstYI r'GATC_y Cuts at: 1972 3066 3570 4198 4565 4950 4967 6419 6505 Size: 1094 504 628 367 385 17 1452 86 Cuts at: 6505 6517 6863 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1094 628 504 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3792 3945 4143 513 Size: 552 939 1788 153 198 3238 Fragments arranged by size: 3238 1788 939 552 198 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2947 487 Size: 683 56 1721 4408 Fragments arranged by size: 4408 1721 683 56 BtsI GCAGTG_nn' Cuts at: 2277 2376 2498 2861 3243 3373 3618 4384 5178 Size: 99 122 363 382 130 245 766 794 Cuts at: 5178 5198 2277 Size: 20 3947 Fragments arranged by size: 3947 794 766 382 363 245 130 122 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6868 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4050 4085 529 Size: 35 3486 35 3312 Fragments arranged by size: 3486 3312 35 35 DraI TTT'AAA Cuts at: 4172 4172 Size: 6868 DraIII CAC_nnn'GTG Cuts at: 3087 4038 3087 Size: 951 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5875 809 Size: 507 4559 1802 Fragments arranged by size: 4559 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 2638 2866 Size: 54 1425 50 275 18 607 201 228 Cuts at: 2866 2890 4315 5204 6833 8 Size: 24 1425 889 1629 43 Fragments arranged by size: 1629 1425 1425 889 607 275 228 201 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2866 4315 1537 Size: 275 1054 1449 4090 Fragments arranged by size: 4090 1449 1054 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3423 4798 1353 Size: 2070 1375 3423 Fragments arranged by size: 3423 2070 1375 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5455 5829 5975 387 Size: 1064 138 3866 374 146 1280 Fragments arranged by size: 3866 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2282 2310 2561 3017 3326 3404 Size: 393 588 62 28 251 456 309 78 Cuts at: 3404 3429 3458 3694 3908 4061 4931 5528 6315 Size: 25 29 236 214 153 870 597 787 Cuts at: 6315 6583 1239 Size: 268 1524 Fragments arranged by size: 1524 870 787 597 588 456 393 309 268 251 236 214 153 78 62 29 28 25 EcoNI CCTnn'n_nnAGG Cuts at: 2264 3159 2264 Size: 895 5973 EcoO109I rG'GnC_Cy Cuts at: 2167 2316 2773 2811 3177 2167 Size: 149 457 38 366 5858 Fragments arranged by size: 5858 457 366 149 38 EcoRI G'AATT_C Cuts at: 4177 4555 4177 Size: 378 6490 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3434 3434 Size: 6868 FspI TGC'GCA Cuts at: 5374 5374 Size: 6868 HaeII r_GCGC'y Cuts at: 1678 2032 2185 2830 6015 1678 Size: 354 153 645 3185 2531 Fragments arranged by size: 3185 2531 645 354 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 3045 3077 3153 Size: 32 135 32 806 32 635 32 76 Cuts at: 3153 3185 3496 3528 5509 5541 5583 5615 1373 Size: 32 311 32 1981 32 42 32 2626 Fragments arranged by size: 2626 1981 806 635 311 135 76 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 4212 4458 669 Size: 988 281 1374 900 246 3079 Fragments arranged by size: 3079 1374 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6868 HpaI GTT'AAC Cuts at: 4458 4458 Size: 6868 KasI G'GCGC_C Cuts at: 2181 2181 Size: 6868 KpnI G_GTAC'C Cuts at: 4193 4193 Size: 6868 MfeI C'AATT_G Cuts at: 3624 4467 3624 Size: 843 6025 MluI A'CGCG_T Cuts at: 6744 6744 Size: 6868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3345 3837 5982 6166 1305 Size: 2040 492 2145 184 2007 Fragments arranged by size: 2145 2040 2007 492 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2439 2640 2892 10 Size: 54 1425 343 607 201 252 3986 Fragments arranged by size: 3986 1425 607 343 252 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3147 3791 3872 4826 5185 5344 Size: 648 1242 739 644 81 954 359 159 Cuts at: 5344 518 Size: 2042 Fragments arranged by size: 2042 1242 954 739 648 644 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2606 3375 3619 4166 4284 4961 6109 6354 Size: 1000 769 244 547 118 677 1148 245 Cuts at: 6354 1606 Size: 2120 Fragments arranged by size: 2120 1148 1000 769 677 547 245 244 118 NarI GG'CG_CC Cuts at: 2182 2182 Size: 6868 NcoI C'CATG_G Cuts at: 513 1065 2004 3792 3945 513 Size: 552 939 1788 153 3436 Fragments arranged by size: 3436 1788 939 552 153 NdeI CA'TA_TG Cuts at: 387 387 Size: 6868 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6868 NotI GC'GGCC_GC Cuts at: 4315 4315 Size: 6868 NspI r_CATG'y Cuts at: 1132 1229 4070 4111 4226 5771 1132 Size: 97 2841 41 115 1545 2229 Fragments arranged by size: 2841 2229 1545 115 97 41 PasI CC'CwG_GG Cuts at: 1348 2107 3115 1348 Size: 759 1008 5101 Fragments arranged by size: 5101 1008 759 PciI A'CATG_T Cuts at: 4066 4107 5767 4066 Size: 41 1660 5167 Fragments arranged by size: 5167 1660 41 PflMI CCAn_nnn'nTGG Cuts at: 1108 3672 1108 Size: 2564 4304 PfoI T'CCnGG_A Cuts at: 2396 2396 Size: 6868 PmeI GTTT'AAAC Cuts at: 4172 4172 Size: 6868 PmlI CAC'GTG Cuts at: 2725 2725 Size: 6868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4937 4969 6450 6482 4937 Size: 32 1481 32 5323 Fragments arranged by size: 5323 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2316 2773 3177 2316 Size: 457 404 6007 Fragments arranged by size: 6007 457 404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6868 PsiI TTA'TAA Cuts at: 4438 4438 Size: 6868 PstI C_TGCA'G Cuts at: 830 2460 4220 830 Size: 1630 1760 3478 Fragments arranged by size: 3478 1760 1630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6868 PvuII CAG'CTG Cuts at: 2606 3619 4166 2606 Size: 1013 547 5308 Fragments arranged by size: 5308 1013 547 SalI G'TCGA_C Cuts at: 1936 4210 1936 Size: 2274 4594 SbfI CC_TGCA'GG Cuts at: 4220 4220 Size: 6868 ScaI AGT'ACT Cuts at: 2567 5116 6828 2567 Size: 2549 1712 2607 Fragments arranged by size: 2607 2549 1712 SfcI C'TryA_G Cuts at: 826 1045 2456 4003 4216 5351 6032 6223 826 Size: 219 1411 1547 213 1135 681 191 1471 Fragments arranged by size: 1547 1471 1411 1135 681 219 213 191 SfoI GGC'GCC Cuts at: 2183 2183 Size: 6868 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 SgrAI Cr'CCGG_yG Cuts at: 1700 2987 1700 Size: 1287 5581 SmaI CCC'GGG Cuts at: 1872 4195 1872 Size: 2323 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 3500 4972 5873 6135 820 Size: 197 926 816 741 1472 901 262 1553 Fragments arranged by size: 1553 1472 926 901 816 741 262 197 SnaBI TAC'GTA Cuts at: 493 4659 493 Size: 4166 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6868 SphI G_CATG'C Cuts at: 1132 4226 1132 Size: 3094 3774 SspI AAT'ATT Cuts at: 5 52 4792 5 Size: 47 4740 2081 Fragments arranged by size: 4740 2081 47 StuI AGG'CCT Cuts at: 1517 2897 1517 Size: 1380 5488 StyI C'CwwG_G Cuts at: 513 1065 2004 2163 3354 3792 3945 4307 513 Size: 552 939 159 1191 438 153 362 3074 Fragments arranged by size: 3074 1191 939 552 438 362 159 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2367 2729 2816 2890 3159 3553 4895 Size: 1255 787 362 87 74 269 394 1342 Cuts at: 4895 4912 5065 5250 5695 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1342 1255 787 445 394 362 269 185 153 87 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 2565 3640 Size: 274 80 33 51 1443 246 342 1075 Cuts at: 3640 5114 6826 96 Size: 1474 1712 138 Fragments arranged by size: 1712 1474 1443 1075 342 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 3274 3306 3794 3826 6828 6860 2342 Size: 32 900 32 488 32 3002 32 2350 Fragments arranged by size: 3002 2350 900 488 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2429 1316 Size: 1113 5755 XbaI T'CTAG_A Cuts at: 4204 4204 Size: 6868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3122 3122 Size: 6868 XhoI C'TCGA_G Cuts at: 1943 3500 1943 Size: 1557 5311 XmaI C'CCGG_G Cuts at: 1870 4193 1870 Size: 2323 4545 XmnI GAAnn'nnTTC Cuts at: 3773 4997 3773 Size: 1224 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4676 276 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI