(Circular) (Six-base) MAPSORT of: pFN21AB5714.seq Check: 2368 from: 1 to: 6868 pFN21AB5714 6868 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3642 3642 Size: 6868 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4678 278 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 4211 1937 Size: 382 1892 4594 Fragments arranged by size: 4594 1892 382 Acc65I G'GTAC_C Cuts at: 4189 4189 Size: 6868 AclI AA'CG_TT Cuts at: 1563 4995 5368 1563 Size: 3432 373 3063 Fragments arranged by size: 3432 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4931 6315 6583 1632 Size: 3299 1384 268 1917 Fragments arranged by size: 3299 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6671 AflIII A'CryG_T Cuts at: 1203 5767 6744 1203 Size: 4564 977 1327 Fragments arranged by size: 4564 1327 977 AgeI A'CCGG_T Cuts at: 5699 5699 Size: 6868 AhdI GACnn_n'nnGTC Cuts at: 5597 5597 Size: 6868 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6868 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2178 1184 Size: 994 5874 AlwNI CAG_nnn'CTG Cuts at: 3013 3046 4611 6183 3013 Size: 33 1565 1572 3698 Fragments arranged by size: 3698 1572 1565 33 ApaLI G'TGCA_C Cuts at: 4925 6081 4925 Size: 1156 5712 ApoI r'AATT_y Cuts at: 1493 3267 3981 4177 4403 4555 6664 1493 Size: 1774 714 196 226 152 2109 1697 Fragments arranged by size: 2109 1774 1697 714 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 5422 160 Size: 872 4390 1606 Fragments arranged by size: 4390 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 AvaI C'yCGr_G Cuts at: 1870 1943 4193 1870 Size: 73 2250 4545 Fragments arranged by size: 4545 2250 73 BamHI G'GATC_C Cuts at: 4198 4565 4198 Size: 367 6501 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4189 5644 618 Size: 325 217 627 2402 1455 1842 Fragments arranged by size: 2402 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2186 4187 1391 Size: 795 2001 4072 Fragments arranged by size: 4072 2001 795 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 3576 3744 928 Size: 971 268 1409 168 4052 Fragments arranged by size: 4052 1409 971 268 168 BbvCI CC'TCA_GC Cuts at: 3888 3888 Size: 6868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5059 5093 1903 Size: 34 50 34 3038 34 3678 Fragments arranged by size: 3678 3038 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4760 5970 4760 Size: 1210 5658 BclI T'GATC_A Cuts at: 1548 3850 4086 1548 Size: 2302 236 4330 Fragments arranged by size: 4330 2302 236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5479 136 Size: 107 122 71 631 605 3807 1525 Fragments arranged by size: 3807 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6863 6863 Size: 6868 BlpI GC'TnA_GC Cuts at: 4285 4285 Size: 6868 Bme1580I G_kGCm'C Cuts at: 1790 3526 4929 6085 1790 Size: 1736 1403 1156 2573 Fragments arranged by size: 2573 1736 1403 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6868 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2841 3400 4599 Size: 416 53 83 399 24 1424 559 1199 Cuts at: 4599 5557 442 Size: 958 1753 Fragments arranged by size: 1753 1424 1199 958 559 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2502 2731 3301 5528 1239 Size: 1263 229 570 2227 2579 Fragments arranged by size: 2579 2227 1263 570 229 Bpu10I CC'TnA_GC Cuts at: 2259 3888 2259 Size: 1629 5239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4993 5858 6156 4993 Size: 865 298 5705 Fragments arranged by size: 5705 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3179 5531 882 Size: 617 1680 2352 2219 Fragments arranged by size: 2352 2219 1680 617 BsaAI yAC'GTr Cuts at: 493 1204 3289 4659 493 Size: 711 2085 1370 2702 Fragments arranged by size: 2702 2085 1370 711 BsaBI GATnn'nnATC Cuts at: 3120 3810 6862 3120 Size: 690 3052 3126 Fragments arranged by size: 3126 3052 690 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4675 5057 5692 275 Size: 53 83 186 936 3142 382 635 1451 Fragments arranged by size: 3142 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2895 5301 5699 5973 6120 6531 2895 Size: 2406 398 274 147 411 3232 Fragments arranged by size: 3232 2406 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2475 2505 3299 3329 1178 Size: 30 165 30 1072 30 794 30 4717 Fragments arranged by size: 4717 1072 794 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6868 BseYI C'CCAG_C Cuts at: 2377 3136 3299 6071 6567 2377 Size: 759 163 2772 496 2678 Fragments arranged by size: 2772 2678 759 496 163 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3021 3353 1163 Size: 573 1285 332 4678 Fragments arranged by size: 4678 1285 573 332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2103 4318 5079 6107 1540 Size: 275 186 102 2215 761 1028 2301 Fragments arranged by size: 2301 2215 1028 761 275 186 102 BsiHKAI G_wGCw'C Cuts at: 2434 4929 5014 6085 2434 Size: 2495 85 1071 3217 Fragments arranged by size: 3217 2495 1071 85 BsmI GAATG_Cn' Cuts at: 2742 3303 3380 3592 4379 4472 2742 Size: 561 77 212 787 93 5138 Fragments arranged by size: 5138 787 561 212 93 77 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2434 3526 4187 4929 5014 6085 Size: 399 396 248 1092 661 742 85 1071 Cuts at: 6085 1391 Size: 2174 Fragments arranged by size: 2174 1092 1071 742 661 399 396 248 85 BspEI T'CCGG_A Cuts at: 6531 6531 Size: 6868 BspHI T'CATG_A Cuts at: 2449 4757 6487 2449 Size: 2308 1730 2830 Fragments arranged by size: 2830 2308 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2964 3642 4040 4223 844 Size: 2120 678 398 183 3489 Fragments arranged by size: 3489 2120 678 398 183 BsrBI CCG'CTC Cuts at: 1394 1823 4755 1394 Size: 429 2932 3507 Fragments arranged by size: 3507 2932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3510 5363 5537 65 Size: 1183 2262 1853 174 1396 Fragments arranged by size: 2262 1853 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2873 5512 5699 1700 Size: 110 1063 2639 187 2869 Fragments arranged by size: 2869 2639 1063 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6868 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6868 BssSI C'ACGA_G Cuts at: 4928 5940 4928 Size: 1012 5856 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6868 BstBI TT'CG_AA Cuts at: 4111 5672 4111 Size: 1561 5307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6868 BstYI r'GATC_y Cuts at: 1972 2549 3364 4198 4565 4950 4967 6419 6505 Size: 577 815 834 367 385 17 1452 86 Cuts at: 6505 6517 6863 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 834 815 577 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6316 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6129 Fragments arranged by size: 6129 683 56 BtsI GCAGTG_nn' Cuts at: 4089 4384 5178 5198 4089 Size: 295 794 20 5759 Fragments arranged by size: 5759 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6868 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6833 DraI TTT'AAA Cuts at: 4172 4172 Size: 6868 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5875 809 Size: 507 4559 1802 Fragments arranged by size: 4559 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2100 2905 Size: 54 1425 50 275 18 231 39 805 Cuts at: 2905 3093 3628 4315 5204 6833 8 Size: 188 535 687 889 1629 43 Fragments arranged by size: 1629 1425 889 805 687 535 275 231 188 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2100 4315 1537 Size: 275 288 2215 4090 Fragments arranged by size: 4090 2215 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 3920 4798 1353 Size: 858 1709 878 3423 Fragments arranged by size: 3423 1709 878 858 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5455 5829 5975 387 Size: 1064 138 3866 374 146 1280 Fragments arranged by size: 3866 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 2731 3301 4931 5528 6315 6583 Size: 393 870 229 570 1630 597 787 268 Cuts at: 6583 1239 Size: 1524 Fragments arranged by size: 1630 1524 870 787 597 570 393 268 229 EcoO109I rG'GnC_Cy Cuts at: 3010 3010 Size: 6868 EcoRI G'AATT_C Cuts at: 4177 4555 4177 Size: 378 6490 EcoRV GAT'ATC Cuts at: 2566 3703 2566 Size: 1137 5731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 6868 FspI TGC'GCA Cuts at: 5374 5374 Size: 6868 HaeII r_GCGC'y Cuts at: 1678 2074 6015 1678 Size: 396 3941 2531 Fragments arranged by size: 3941 2531 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 5509 5541 5583 Size: 32 135 32 1112 32 2793 32 42 Cuts at: 5583 5615 1373 Size: 32 2626 Fragments arranged by size: 2793 2626 1112 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3502 4212 4458 669 Size: 988 281 1564 710 246 3079 Fragments arranged by size: 3079 1564 988 710 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6868 HpaI GTT'AAC Cuts at: 3502 4458 3502 Size: 956 5912 KpnI G_GTAC'C Cuts at: 4193 4193 Size: 6868 MfeI C'AATT_G Cuts at: 2370 2425 2868 4467 2370 Size: 55 443 1599 4771 Fragments arranged by size: 4771 1599 443 55 MluI A'CGCG_T Cuts at: 6744 6744 Size: 6868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2527 2662 3862 4045 5982 6166 1305 Size: 1222 135 1200 183 1937 184 2007 Fragments arranged by size: 2007 1937 1222 1200 184 183 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 2907 3095 10 Size: 54 1425 343 231 844 188 3783 Fragments arranged by size: 3783 1425 844 343 231 188 54 MslI CAynn'nnrTG Cuts at: 518 1166 2160 4148 4826 5185 5344 518 Size: 648 994 1988 678 359 159 2042 Fragments arranged by size: 2042 1988 994 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2232 4284 4961 6109 6354 1606 Size: 626 2052 677 1148 245 2120 Fragments arranged by size: 2120 2052 1148 677 626 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6316 NdeI CA'TA_TG Cuts at: 387 387 Size: 6868 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6868 NotI GC'GGCC_GC Cuts at: 2100 4315 2100 Size: 2215 4653 NspI r_CATG'y Cuts at: 1132 1229 2159 4226 5771 1132 Size: 97 930 2067 1545 2229 Fragments arranged by size: 2229 2067 1545 930 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6868 PciI A'CATG_T Cuts at: 5767 5767 Size: 6868 PflMI CCAn_nnn'nTGG Cuts at: 1108 3715 1108 Size: 2607 4261 PmeI GTTT'AAAC Cuts at: 4172 4172 Size: 6868 PmlI CAC'GTG Cuts at: 3289 3289 Size: 6868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4937 4969 6450 6482 4937 Size: 32 1481 32 5323 Fragments arranged by size: 5323 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3010 3010 Size: 6868 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6868 PsiI TTA'TAA Cuts at: 4438 4438 Size: 6868 PstI C_TGCA'G Cuts at: 830 2245 4220 830 Size: 1415 1975 3478 Fragments arranged by size: 3478 1975 1415 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6868 PvuII CAG'CTG Cuts at: 2232 2232 Size: 6868 SalI G'TCGA_C Cuts at: 1936 4210 1936 Size: 2274 4594 SbfI CC_TGCA'GG Cuts at: 4220 4220 Size: 6868 ScaI AGT'ACT Cuts at: 5116 6828 5116 Size: 1712 5156 SexAI A'CCwGG_T Cuts at: 4080 4080 Size: 6868 SfcI C'TryA_G Cuts at: 826 1045 2241 2341 4216 5351 6032 6223 826 Size: 219 1196 100 1875 1135 681 191 1471 Fragments arranged by size: 1875 1471 1196 1135 681 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6868 SmaI CCC'GGG Cuts at: 1872 4195 1872 Size: 2323 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4972 5873 6135 820 Size: 197 926 3029 901 262 1553 Fragments arranged by size: 3029 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4659 493 Size: 4166 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6868 SphI G_CATG'C Cuts at: 1132 4226 1132 Size: 3094 3774 SspI AAT'ATT Cuts at: 5 52 4792 5 Size: 47 4740 2081 Fragments arranged by size: 4740 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6868 StyI C'CwwG_G Cuts at: 513 1065 2499 3081 4307 513 Size: 552 1434 582 1226 3074 Fragments arranged by size: 3074 1434 1226 582 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2841 3419 3536 4895 4912 5065 Size: 1255 543 718 578 117 1359 17 153 Cuts at: 5065 5250 5695 325 Size: 185 445 1498 Fragments arranged by size: 1498 1359 1255 718 578 543 445 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5114 6826 96 Size: 274 80 33 51 1443 3137 1712 138 Fragments arranged by size: 3137 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3299 3331 6828 6860 3299 Size: 32 3497 32 3307 Fragments arranged by size: 3497 3307 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6868 XbaI T'CTAG_A Cuts at: 4204 4204 Size: 6868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3088 3088 Size: 6868 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6868 XmaI C'CCGG_G Cuts at: 1870 4193 1870 Size: 2323 4545 XmnI GAAnn'nnTTC Cuts at: 3380 3741 4997 3380 Size: 361 1256 5251 Fragments arranged by size: 5251 1256 361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4676 276 Size: 53 83 186 936 3142 2468 Fragments arranged by size: 3142 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI