(Circular) (Six-base) MAPSORT of: pFN21AB5709.seq Check: 5868 from: 1 to: 7057 pFN21AB5709 7057 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4867 278 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4400 1937 Size: 2463 4594 Acc65I G'GTAC_C Cuts at: 4378 4378 Size: 7057 AclI AA'CG_TT Cuts at: 1563 5184 5557 1563 Size: 3621 373 3063 Fragments arranged by size: 3621 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 3640 3948 4057 4340 5120 6504 6772 Size: 452 1556 308 109 283 780 1384 268 Cuts at: 6772 1632 Size: 1917 Fragments arranged by size: 1917 1556 1384 780 452 308 283 268 109 AfeI AGC'GCT Cuts at: 3833 3833 Size: 7057 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6860 AflIII A'CryG_T Cuts at: 1203 2271 5956 6933 1203 Size: 1068 3685 977 1327 Fragments arranged by size: 3685 1327 1068 977 AgeI A'CCGG_T Cuts at: 5888 5888 Size: 7057 AhdI GACnn_n'nnGTC Cuts at: 5786 5786 Size: 7057 AleI CACnn'nnGTG Cuts at: 1166 3797 1166 Size: 2631 4426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7057 AlwNI CAG_nnn'CTG Cuts at: 4193 4800 6372 4193 Size: 607 1572 4878 Fragments arranged by size: 4878 1572 607 ApaI G_GGCC'C Cuts at: 3305 3813 3873 3305 Size: 508 60 6489 Fragments arranged by size: 6489 508 60 ApaLI G'TGCA_C Cuts at: 5114 6270 5114 Size: 1156 5901 ApoI r'AATT_y Cuts at: 1493 4366 4592 4744 6853 1493 Size: 2873 226 152 2109 1697 Fragments arranged by size: 2873 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5611 160 Size: 872 4579 1606 Fragments arranged by size: 4579 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 AvaI C'yCGr_G Cuts at: 1870 1943 3264 4382 1870 Size: 73 1321 1118 4545 Fragments arranged by size: 4545 1321 1118 73 BamHI G'GATC_C Cuts at: 2748 4387 4754 2748 Size: 1639 367 5051 Fragments arranged by size: 5051 1639 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2458 4378 5833 618 Size: 325 217 627 671 1920 1455 1842 Fragments arranged by size: 1920 1842 1455 671 627 325 217 BanII G_rGCy'C Cuts at: 1391 2583 3305 3687 3813 3873 4203 4218 4376 Size: 1192 722 382 126 60 330 15 158 Cuts at: 4376 1391 Size: 4072 Fragments arranged by size: 4072 1192 722 382 330 158 126 60 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 3903 4089 928 Size: 971 231 1773 186 3896 Fragments arranged by size: 3896 1773 971 231 186 BbvCI CC'TCA_GC Cuts at: 3324 3540 4194 3324 Size: 216 654 6187 Fragments arranged by size: 6187 654 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5248 5282 1903 Size: 34 3311 34 3678 Fragments arranged by size: 3678 3311 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4949 6159 4949 Size: 1210 5847 BclI T'GATC_A Cuts at: 1548 3075 1548 Size: 1527 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5668 136 Size: 107 122 71 631 605 3996 1525 Fragments arranged by size: 3996 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3009 7052 3009 Size: 4043 3014 BlpI GC'TnA_GC Cuts at: 4474 4474 Size: 7057 Bme1580I G_kGCm'C Cuts at: 1790 3305 3813 3873 4352 5118 6274 1790 Size: 1515 508 60 479 766 1156 2573 Fragments arranged by size: 2573 1515 1156 766 508 479 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3734 4788 5746 Size: 416 53 83 399 24 2317 1054 958 Cuts at: 5746 442 Size: 1753 Fragments arranged by size: 2317 1753 1054 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7057 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3889 3889 Size: 7057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2868 3239 3368 3701 3889 5717 1239 Size: 1629 371 129 333 188 1828 2579 Fragments arranged by size: 2579 1828 1629 371 333 188 129 Bpu10I CC'TnA_GC Cuts at: 2633 3324 3540 4194 2633 Size: 691 216 654 5496 Fragments arranged by size: 5496 691 654 216 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 3257 3334 4093 5182 6047 6345 2768 Size: 489 77 759 1089 865 298 3480 Fragments arranged by size: 3480 1089 865 759 489 298 77 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5720 882 Size: 617 4221 2219 Fragments arranged by size: 4221 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4848 493 Size: 711 3644 2702 Fragments arranged by size: 3644 2702 711 BsaBI GATnn'nnATC Cuts at: 7051 7051 Size: 7057 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4864 5246 5881 275 Size: 53 83 186 936 3331 382 635 1451 Fragments arranged by size: 3331 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2520 2745 5490 5888 6162 6309 6720 2520 Size: 225 2745 398 274 147 411 2857 Fragments arranged by size: 2857 2745 411 398 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3919 3949 1178 Size: 30 165 30 2516 30 4286 Fragments arranged by size: 4286 2516 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3371 3557 3560 4025 4028 4208 4211 1186 Size: 2185 186 3 465 3 180 3 4032 Fragments arranged by size: 4032 2185 465 186 180 3 3 3 BseYI C'CCAG_C Cuts at: 2651 2918 3225 3686 3814 4057 4095 6260 6756 Size: 267 307 461 128 243 38 2165 496 Cuts at: 6756 2651 Size: 2952 Fragments arranged by size: 2952 2165 496 461 307 267 243 128 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2990 3122 3614 1163 Size: 573 1254 132 492 4606 Fragments arranged by size: 4606 1254 573 492 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4276 4507 5268 6296 1540 Size: 275 186 2275 231 761 1028 2301 Fragments arranged by size: 2301 2275 1028 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 2583 2639 2817 4218 5118 5203 6274 2583 Size: 56 178 1401 900 85 1071 3366 Fragments arranged by size: 3366 1401 1071 900 178 85 56 BsmI GAATG_Cn' Cuts at: 4568 4661 4568 Size: 93 6964 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 2639 2817 3305 3687 3813 3873 Size: 399 793 56 178 488 382 126 60 Cuts at: 3873 4203 4218 4352 4376 5118 5203 6274 1391 Size: 330 15 134 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 793 742 488 399 382 330 178 134 126 85 60 56 24 15 BspEI T'CCGG_A Cuts at: 2520 6720 2520 Size: 4200 2857 BspHI T'CATG_A Cuts at: 4946 6676 4946 Size: 1730 5327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3477 3561 4412 844 Size: 2633 84 851 3489 Fragments arranged by size: 3489 2633 851 84 BsrBI CCG'CTC Cuts at: 1394 1823 3269 3379 4944 1394 Size: 429 1446 110 1565 3507 Fragments arranged by size: 3507 1565 1446 429 110 BsrDI GCAATG_nn' Cuts at: 65 1248 5552 5726 65 Size: 1183 4304 174 1396 Fragments arranged by size: 4304 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5701 5888 1700 Size: 110 3891 187 2869 Fragments arranged by size: 3891 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2759 96 Size: 2663 4394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7057 BssSI C'ACGA_G Cuts at: 5117 6129 5117 Size: 1012 6045 BstAPI GCAn_nnn'nTGC Cuts at: 794 3577 794 Size: 2783 4274 BstBI TT'CG_AA Cuts at: 5861 5861 Size: 7057 BstEII G'GTnAC_C Cuts at: 1184 4127 1184 Size: 2943 4114 BstXI CCAn_nnnn'nTGG Cuts at: 2291 3913 2291 Size: 1622 5435 BstYI r'GATC_y Cuts at: 1972 2068 2748 3009 3390 3724 4387 4754 5139 Size: 96 680 261 381 334 663 367 385 Cuts at: 5139 5156 6608 6694 6706 7052 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 680 663 385 381 367 346 334 261 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2674 3198 3429 3912 513 Size: 552 939 670 524 231 483 3658 Fragments arranged by size: 3658 939 670 552 524 483 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6318 Fragments arranged by size: 6318 683 56 BtsI GCAGTG_nn' Cuts at: 2397 2536 2716 2764 3128 4029 4274 4573 5367 Size: 139 180 48 364 901 245 299 794 Cuts at: 5367 5387 2397 Size: 20 4067 Fragments arranged by size: 4067 901 794 364 299 245 180 139 48 20 ClaI AT'CG_AT Cuts at: 1554 4233 1554 Size: 2679 4378 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7022 DraI TTT'AAA Cuts at: 4361 4361 Size: 7057 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 2480 6064 809 Size: 507 816 348 3584 1802 Fragments arranged by size: 3584 1802 816 507 348 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3270 3823 4273 Size: 54 1425 50 275 18 1440 553 450 Cuts at: 4273 4332 4504 5393 7022 8 Size: 59 172 889 1629 43 Fragments arranged by size: 1629 1440 1425 889 553 450 275 172 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4273 4504 1537 Size: 275 2461 231 4090 Fragments arranged by size: 4090 2461 275 231 EarI CTCTTCn'nnn_ Cuts at: 1353 3185 3739 3912 3996 4206 4987 1353 Size: 1832 554 173 84 210 781 3423 Fragments arranged by size: 3423 1832 781 554 210 173 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5644 6018 6164 387 Size: 1064 138 4055 374 146 1280 Fragments arranged by size: 4055 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2581 4216 2581 Size: 1635 5422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2868 3239 3368 3640 3701 3889 Size: 393 452 784 371 129 272 61 188 Cuts at: 3889 3948 4057 4340 5120 5717 6504 6772 1239 Size: 59 109 283 780 597 787 268 1524 Fragments arranged by size: 1524 787 784 780 597 452 393 371 283 272 268 188 129 109 61 59 EcoNI CCTnn'n_nnAGG Cuts at: 3942 4283 3942 Size: 341 6716 EcoO109I rG'GnC_Cy Cuts at: 2226 3034 3301 3302 3550 3810 3869 3870 2226 Size: 808 267 1 248 260 59 1 5413 Fragments arranged by size: 5413 808 267 260 248 59 1 1 EcoRI G'AATT_C Cuts at: 4366 4744 4366 Size: 378 6679 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3028 3028 Size: 7057 FspI TGC'GCA Cuts at: 3452 5563 3452 Size: 2111 4946 FspAI rTGC'GCAy Cuts at: 3452 3452 Size: 7057 HaeII r_GCGC'y Cuts at: 1678 3835 6204 1678 Size: 2157 2369 2531 Fragments arranged by size: 2531 2369 2157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2293 2325 2679 Size: 32 135 32 488 32 201 32 354 Cuts at: 2679 2711 3272 3304 3716 3748 4291 4323 5698 Size: 32 561 32 412 32 543 32 1375 Cuts at: 5698 5730 5772 5804 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1375 561 543 488 412 354 201 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2470 2872 4401 4647 669 Size: 988 281 532 402 1529 246 3079 Fragments arranged by size: 3079 1529 988 532 402 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7057 HpaI GTT'AAC Cuts at: 4647 4647 Size: 7057 KpnI G_GTAC'C Cuts at: 4382 4382 Size: 7057 MfeI C'AATT_G Cuts at: 4656 4656 Size: 7057 MluI A'CGCG_T Cuts at: 6933 6933 Size: 7057 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 3573 6171 6355 1305 Size: 1035 1233 2598 184 2007 Fragments arranged by size: 2598 2007 1233 1035 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3825 4334 10 Size: 54 1425 343 1993 509 2733 Fragments arranged by size: 2733 1993 1425 509 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 2949 3797 5015 5374 5533 518 Size: 648 1731 52 848 1218 359 159 2042 Fragments arranged by size: 2042 1731 1218 848 648 359 159 52 MspA1I CmG'CkG Cuts at: 1606 2754 3431 3478 3559 3568 3679 4473 5150 Size: 1148 677 47 81 9 111 794 677 Cuts at: 5150 6298 6543 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1148 794 677 677 245 111 81 47 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2674 3912 513 Size: 552 939 670 1238 3658 Fragments arranged by size: 3658 1238 939 670 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7057 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7057 NotI GC'GGCC_GC Cuts at: 4273 4504 4273 Size: 231 6826 NspI r_CATG'y Cuts at: 1132 1229 2275 2948 3853 4415 5960 1132 Size: 97 1046 673 905 562 1545 2229 Fragments arranged by size: 2229 1545 1046 905 673 562 97 PasI CC'CwG_GG Cuts at: 1348 2024 1348 Size: 676 6381 PciI A'CATG_T Cuts at: 2271 5956 2271 Size: 3685 3372 PflMI CCAn_nnn'nTGG Cuts at: 1108 2501 3801 3821 1108 Size: 1393 1300 20 4344 Fragments arranged by size: 4344 1393 1300 20 PfoI T'CCnGG_A Cuts at: 2724 3527 2724 Size: 803 6254 PmeI GTTT'AAAC Cuts at: 4361 4361 Size: 7057 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5126 5158 6639 6671 5126 Size: 32 1481 32 5512 Fragments arranged by size: 5512 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2226 3034 3550 2226 Size: 808 516 5733 Fragments arranged by size: 5733 808 516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7057 PsiI TTA'TAA Cuts at: 4627 4627 Size: 7057 PspOMI G'GGCC_C Cuts at: 3301 3809 3869 3301 Size: 508 60 6489 Fragments arranged by size: 6489 508 60 PstI C_TGCA'G Cuts at: 830 2076 3069 3462 3483 3558 3573 3946 4227 Size: 1246 993 393 21 75 15 373 281 Cuts at: 4227 4409 830 Size: 182 3478 Fragments arranged by size: 3478 1246 993 393 373 281 182 75 21 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7057 PvuII CAG'CTG Cuts at: 3478 3568 3679 3478 Size: 90 111 6856 Fragments arranged by size: 6856 111 90 SacI G_AGCT'C Cuts at: 2583 4218 2583 Size: 1635 5422 SacII CC_GC'GG Cuts at: 3432 3432 Size: 7057 SalI G'TCGA_C Cuts at: 1936 4399 1936 Size: 2463 4594 SanDI GG'GwC_CC Cuts at: 2226 2226 Size: 7057 SapI GCTCTTCn'nnn_ Cuts at: 3739 4206 3739 Size: 467 6590 SbfI CC_TGCA'GG Cuts at: 4409 4409 Size: 7057 ScaI AGT'ACT Cuts at: 5305 7017 5305 Size: 1712 5345 SfcI C'TryA_G Cuts at: 826 1045 2072 2932 3065 3458 3479 3554 3569 Size: 219 1027 860 133 393 21 75 15 Cuts at: 3569 3942 4223 4405 5540 6221 6412 826 Size: 373 281 182 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 860 681 393 373 281 219 191 182 133 75 21 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7057 SmaI CCC'GGG Cuts at: 1872 4384 1872 Size: 2512 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 3236 3264 3313 4108 5161 Size: 197 926 840 453 28 49 795 1053 Cuts at: 5161 6062 6324 820 Size: 901 262 1553 Fragments arranged by size: 1553 1053 926 901 840 795 453 262 197 49 28 SnaBI TAC'GTA Cuts at: 493 4848 493 Size: 4355 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7057 SphI G_CATG'C Cuts at: 1132 3853 4415 1132 Size: 2721 562 3774 Fragments arranged by size: 3774 2721 562 SspI AAT'ATT Cuts at: 5 52 4981 5 Size: 47 4929 2081 Fragments arranged by size: 4929 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7057 StyI C'CwwG_G Cuts at: 513 1065 2004 2230 2462 2595 2674 3018 3423 Size: 552 939 226 232 133 79 344 405 Cuts at: 3423 3912 4496 513 Size: 489 584 3074 Fragments arranged by size: 3074 939 584 552 489 405 344 232 226 133 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2654 2677 2710 3633 3719 5084 5101 Size: 1255 1074 23 33 923 86 1365 17 Cuts at: 5101 5254 5439 5884 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1365 1255 1074 923 445 185 153 86 33 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2759 3984 5303 Size: 274 80 33 51 1443 782 1225 1319 Cuts at: 5303 7015 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1319 1225 782 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3916 3948 7017 7049 3916 Size: 32 3069 32 3924 Fragments arranged by size: 3924 3069 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3643 1316 Size: 2327 4730 XbaI T'CTAG_A Cuts at: 4393 4393 Size: 7057 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3280 2011 Size: 1269 5788 XhoI C'TCGA_G Cuts at: 1943 3264 1943 Size: 1321 5736 XmaI C'CCGG_G Cuts at: 1870 4382 1870 Size: 2512 4545 XmnI GAAnn'nnTTC Cuts at: 2510 4037 5186 2510 Size: 1527 1149 4381 Fragments arranged by size: 4381 1527 1149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4865 276 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI