(Circular) (Six-base) MAPSORT of: pFN21AB5707.seq Check: 8004 from: 1 to: 7051 pFN21AB5707 7051 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3879 3031 Size: 848 6203 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2431 2516 4861 278 Size: 53 83 186 936 895 85 2345 2468 Fragments arranged by size: 2468 2345 936 895 186 85 83 53 AccI GT'mk_AC Cuts at: 1937 2516 3383 4394 1937 Size: 579 867 1011 4594 Fragments arranged by size: 4594 1011 867 579 Acc65I G'GTAC_C Cuts at: 4372 4372 Size: 7051 AclI AA'CG_TT Cuts at: 1563 5178 5551 1563 Size: 3615 373 3063 Fragments arranged by size: 3615 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 2667 3637 4009 4284 5114 6498 6766 Size: 752 283 970 372 275 830 1384 268 Cuts at: 6766 1632 Size: 1917 Fragments arranged by size: 1917 1384 970 830 752 372 283 275 268 AflII C'TTAA_G Cuts at: 820 1017 2291 820 Size: 197 1274 5580 Fragments arranged by size: 5580 1274 197 AflIII A'CryG_T Cuts at: 1203 2199 5950 6927 1203 Size: 996 3751 977 1327 Fragments arranged by size: 3751 1327 996 977 AgeI A'CCGG_T Cuts at: 5882 5882 Size: 7051 AhdI GACnn_n'nnGTC Cuts at: 5780 5780 Size: 7051 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7051 AlwNI CAG_nnn'CTG Cuts at: 3850 4794 6366 3850 Size: 944 1572 4535 Fragments arranged by size: 4535 1572 944 ApaLI G'TGCA_C Cuts at: 5108 6264 5108 Size: 1156 5895 ApoI r'AATT_y Cuts at: 1493 2588 2879 3600 4360 4586 4738 6847 1493 Size: 1095 291 721 760 226 152 2109 1697 Fragments arranged by size: 2109 1697 1095 760 721 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 5605 160 Size: 872 4573 1606 Fragments arranged by size: 4573 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 AvaI C'yCGr_G Cuts at: 1870 1943 4376 1870 Size: 73 2433 4545 Fragments arranged by size: 4545 2433 73 BamHI G'GATC_C Cuts at: 4381 4748 4381 Size: 367 6684 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4372 5827 618 Size: 325 217 627 2585 1455 1842 Fragments arranged by size: 2585 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2827 2954 3378 3906 4370 1391 Size: 1436 127 424 528 464 4072 Fragments arranged by size: 4072 1436 528 464 424 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3297 928 Size: 971 1398 4682 Fragments arranged by size: 4682 1398 971 BbvCI CC'TCA_GC Cuts at: 2270 2546 3345 2270 Size: 276 799 5976 Fragments arranged by size: 5976 799 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5242 5276 1903 Size: 34 3305 34 3678 Fragments arranged by size: 3678 3305 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2247 4943 6153 2247 Size: 2696 1210 3145 Fragments arranged by size: 3145 2696 1210 BclI T'GATC_A Cuts at: 1548 2034 1548 Size: 486 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5662 136 Size: 107 122 71 631 605 3990 1525 Fragments arranged by size: 3990 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3064 7046 3064 Size: 3982 3069 BlpI GC'TnA_GC Cuts at: 2332 4468 2332 Size: 2136 4915 Bme1580I G_kGCm'C Cuts at: 1790 5112 6268 1790 Size: 3322 1156 2573 Fragments arranged by size: 3322 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2200 4210 1318 Size: 882 2010 4159 Fragments arranged by size: 4159 2010 882 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4782 5740 442 Size: 416 53 83 399 24 3365 958 1753 Fragments arranged by size: 3365 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7051 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2129 3360 3903 2129 Size: 1231 543 5277 Fragments arranged by size: 5277 1231 543 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 3371 5711 1239 Size: 866 1266 2340 2579 Fragments arranged by size: 2579 2340 1266 866 Bpu10I CC'TnA_GC Cuts at: 2270 2546 2583 3345 2270 Size: 276 37 762 5976 Fragments arranged by size: 5976 762 276 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2968 3404 5176 6041 6339 2968 Size: 436 1772 865 298 3680 Fragments arranged by size: 3680 1772 865 436 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2796 5714 882 Size: 617 1297 2918 2219 Fragments arranged by size: 2918 2219 1297 617 BsaAI yAC'GTr Cuts at: 493 1204 3630 4842 493 Size: 711 2426 1212 2702 Fragments arranged by size: 2702 2426 1212 711 BsaBI GATnn'nnATC Cuts at: 7045 7045 Size: 7051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2428 2513 3896 4858 Size: 53 83 186 936 895 85 1383 962 Cuts at: 4858 5240 5875 275 Size: 382 635 1451 Fragments arranged by size: 1451 1383 962 936 895 635 382 186 85 83 53 BsaWI w'CCGG_w Cuts at: 3460 5484 5882 6156 6303 6714 3460 Size: 2024 398 274 147 411 3797 Fragments arranged by size: 3797 2024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2207 2237 2927 2957 3831 Size: 30 165 30 804 30 690 30 874 Cuts at: 3831 3861 1178 Size: 30 4368 Fragments arranged by size: 4368 874 804 690 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2944 3269 1186 Size: 1042 716 325 4968 Fragments arranged by size: 4968 1042 716 325 BseYI C'CCAG_C Cuts at: 3170 6254 6750 3170 Size: 3084 496 3471 Fragments arranged by size: 3471 3084 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3857 1163 Size: 573 2121 4357 Fragments arranged by size: 4357 2121 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4501 5262 6290 1540 Size: 275 186 2500 761 1028 2301 Fragments arranged by size: 2500 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2531 2584 2861 2954 5112 5197 6268 2531 Size: 53 277 93 2158 85 1071 3314 Fragments arranged by size: 3314 2158 1071 277 93 85 53 BsmI GAATG_Cn' Cuts at: 4328 4562 4655 4328 Size: 234 93 6724 Fragments arranged by size: 6724 234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2102 2190 4216 2102 Size: 88 2026 4937 Fragments arranged by size: 4937 2026 88 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2531 2584 2827 2861 2954 3378 3906 Size: 399 741 53 243 34 93 424 528 Cuts at: 3906 4370 5112 5197 6268 1391 Size: 464 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 528 464 424 399 243 93 85 53 34 BspEI T'CCGG_A Cuts at: 3460 6714 3460 Size: 3254 3797 BspHI T'CATG_A Cuts at: 3103 4158 4940 6670 3103 Size: 1055 782 1730 3484 Fragments arranged by size: 3484 1730 1055 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2528 3031 3879 4190 4406 844 Size: 1684 503 848 311 216 3489 Fragments arranged by size: 3489 1684 848 503 311 216 BsrBI CCG'CTC Cuts at: 1394 1823 4938 1394 Size: 429 3115 3507 Fragments arranged by size: 3507 3115 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2403 4056 5546 5720 65 Size: 1183 1155 1653 1490 174 1396 Fragments arranged by size: 1653 1490 1396 1183 1155 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5695 5882 1700 Size: 110 3885 187 2869 Fragments arranged by size: 3885 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2400 96 Size: 2304 4747 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7051 BssSI C'ACGA_G Cuts at: 2059 5111 6123 2059 Size: 3052 1012 2987 Fragments arranged by size: 3052 2987 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4148 794 Size: 3354 3697 BstBI TT'CG_AA Cuts at: 5855 5855 Size: 7051 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7051 BstXI CCAn_nnnn'nTGG Cuts at: 2607 4250 2607 Size: 1643 5408 BstYI r'GATC_y Cuts at: 1972 2707 3064 3792 4381 4748 5133 5150 6602 Size: 735 357 728 589 367 385 17 1452 Cuts at: 6602 6688 6700 7046 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 735 728 589 385 367 357 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3384 3384 Size: 7051 BtgI C'CryG_G Cuts at: 513 1065 2004 2532 2769 513 Size: 552 939 528 237 4795 Fragments arranged by size: 4795 939 552 528 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3141 487 Size: 683 56 1915 4397 Fragments arranged by size: 4397 1915 683 56 BtsI GCAGTG_nn' Cuts at: 4567 5361 5381 4567 Size: 794 20 6237 Fragments arranged by size: 6237 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7051 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3078 3113 529 Size: 35 2514 35 4467 Fragments arranged by size: 4467 2514 35 35 DraI TTT'AAA Cuts at: 4355 4355 Size: 7051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6058 809 Size: 507 4742 1802 Fragments arranged by size: 4742 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3935 4498 5387 Size: 54 1425 50 275 18 2105 563 889 Cuts at: 5387 7016 8 Size: 1629 43 Fragments arranged by size: 2105 1629 1425 889 563 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4498 1537 Size: 275 2686 4090 Fragments arranged by size: 4090 2686 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2780 2815 4132 4981 1353 Size: 1427 35 1317 849 3423 Fragments arranged by size: 3423 1427 1317 849 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5638 6012 6158 387 Size: 1064 138 4049 374 146 1280 Fragments arranged by size: 4049 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2952 2952 Size: 7051 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2384 2667 3371 3637 4009 4284 Size: 393 473 279 283 704 266 372 275 Cuts at: 4284 5114 5711 6498 6766 1239 Size: 830 597 787 268 1524 Fragments arranged by size: 1524 830 787 704 597 473 393 372 283 279 275 268 266 EcoO109I rG'GnC_Cy Cuts at: 3221 3221 Size: 7051 EcoRI G'AATT_C Cuts at: 4360 4738 4360 Size: 378 6673 EcoRV GAT'ATC Cuts at: 2458 2458 Size: 7051 FspI TGC'GCA Cuts at: 5557 5557 Size: 7051 HaeII r_GCGC'y Cuts at: 1678 6198 1678 Size: 4520 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2126 2158 2207 2239 2645 Size: 32 135 32 554 32 49 32 406 Cuts at: 2645 2677 2774 2806 2927 2959 4153 4185 5692 Size: 32 97 32 121 32 1194 32 1507 Cuts at: 5692 5724 5766 5798 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1507 1194 554 406 135 121 97 49 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3571 4395 4641 669 Size: 988 281 1633 824 246 3079 Fragments arranged by size: 3079 1633 988 824 281 246 HindIII A'AGCT_T Cuts at: 748 3432 4098 748 Size: 2684 666 3701 Fragments arranged by size: 3701 2684 666 HpaI GTT'AAC Cuts at: 4641 4641 Size: 7051 KpnI G_GTAC'C Cuts at: 4376 4376 Size: 7051 MfeI C'AATT_G Cuts at: 4650 4650 Size: 7051 MluI A'CGCG_T Cuts at: 6927 6927 Size: 7051 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2729 6165 6349 1305 Size: 1424 3436 184 2007 Fragments arranged by size: 3436 2007 1424 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5229 Fragments arranged by size: 5229 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5009 5368 5527 518 Size: 648 3843 359 159 2042 Fragments arranged by size: 3843 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 2269 2840 2971 3349 3617 4467 5144 Size: 615 48 571 131 378 268 850 677 Cuts at: 5144 6292 6537 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 850 677 615 571 378 268 245 131 48 NcoI C'CATG_G Cuts at: 513 1065 2004 2769 513 Size: 552 939 765 4795 Fragments arranged by size: 4795 939 765 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7051 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7051 NotI GC'GGCC_GC Cuts at: 4498 4498 Size: 7051 NspI r_CATG'y Cuts at: 1132 1229 4409 5954 1132 Size: 97 3180 1545 2229 Fragments arranged by size: 3180 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3143 1348 Size: 1795 5256 PciI A'CATG_T Cuts at: 5950 5950 Size: 7051 PflMI CCAn_nnn'nTGG Cuts at: 1108 3197 1108 Size: 2089 4962 PfoI T'CCnGG_A Cuts at: 3689 3689 Size: 7051 PmeI GTTT'AAAC Cuts at: 4355 4355 Size: 7051 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5120 5152 6633 6665 5120 Size: 32 1481 32 5506 Fragments arranged by size: 5506 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7051 PsiI TTA'TAA Cuts at: 4044 4621 4044 Size: 577 6474 PstI C_TGCA'G Cuts at: 830 3846 4014 4403 830 Size: 3016 168 389 3478 Fragments arranged by size: 3478 3016 389 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7051 PvuII CAG'CTG Cuts at: 2221 2269 3349 3617 2221 Size: 48 1080 268 5655 Fragments arranged by size: 5655 1080 268 48 SacI G_AGCT'C Cuts at: 2954 2954 Size: 7051 SalI G'TCGA_C Cuts at: 1936 4393 1936 Size: 2457 4594 SapI GCTCTTCn'nnn_ Cuts at: 2815 4132 2815 Size: 1317 5734 SbfI CC_TGCA'GG Cuts at: 4403 4403 Size: 7051 ScaI AGT'ACT Cuts at: 5299 7011 5299 Size: 1712 5339 SexAI A'CCwGG_T Cuts at: 2519 2519 Size: 7051 SfcI C'TryA_G Cuts at: 826 1045 3842 4010 4399 5534 6215 6406 826 Size: 219 2797 168 389 1135 681 191 1471 Fragments arranged by size: 2797 1471 1135 681 389 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7051 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7051 SmaI CCC'GGG Cuts at: 1872 4378 1872 Size: 2506 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 2947 3419 5155 6056 6318 Size: 197 926 348 656 472 1736 901 262 Cuts at: 6318 820 Size: 1553 Fragments arranged by size: 1736 1553 926 901 656 472 348 262 197 SnaBI TAC'GTA Cuts at: 493 4842 493 Size: 4349 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7051 SphI G_CATG'C Cuts at: 1132 4409 1132 Size: 3277 3774 SspI AAT'ATT Cuts at: 5 52 4295 4975 5 Size: 47 4243 680 2081 Fragments arranged by size: 4243 2081 680 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7051 StyI C'CwwG_G Cuts at: 513 1065 2004 2769 4490 513 Size: 552 939 765 1721 3074 Fragments arranged by size: 3074 1721 939 765 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3681 3850 5078 5095 5248 5433 5878 Size: 1255 2101 169 1228 17 153 185 445 Cuts at: 5878 325 Size: 1498 Fragments arranged by size: 2101 1498 1255 1228 445 185 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2565 4310 Size: 274 80 33 51 1443 423 165 1745 Cuts at: 4310 5297 7009 96 Size: 987 1712 138 Fragments arranged by size: 1745 1712 1443 987 423 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7011 7043 7011 Size: 32 7019 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7051 XbaI T'CTAG_A Cuts at: 4387 4387 Size: 7051 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7051 XmaI C'CCGG_G Cuts at: 1870 4376 1870 Size: 2506 4545 XmnI GAAnn'nnTTC Cuts at: 5180 5180 Size: 7051 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2429 2514 4859 276 Size: 53 83 186 936 895 85 2345 2468 Fragments arranged by size: 2468 2345 936 895 186 85 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI