(Circular) (Six-base) MAPSORT of: pFN21AB8507.seq Check: 9909 from: 1 to: 7528 pFN21AB8507 7528 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2110 4589 2110 Size: 2479 5049 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3990 5338 278 Size: 53 83 186 936 2454 1348 2468 Fragments arranged by size: 2468 2454 1348 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2769 4871 1937 Size: 832 2102 4594 Fragments arranged by size: 4594 2102 832 Acc65I G'GTAC_C Cuts at: 3546 4389 4849 3546 Size: 843 460 6225 Fragments arranged by size: 6225 843 460 AclI AA'CG_TT Cuts at: 1563 5655 6028 1563 Size: 4092 373 3063 Fragments arranged by size: 4092 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 3572 3716 3986 5591 6975 7243 1632 Size: 671 1269 144 270 1605 1384 268 1917 Fragments arranged by size: 1917 1605 1384 1269 671 270 268 144 AfeI AGC'GCT Cuts at: 3644 3644 Size: 7528 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7331 AflIII A'CryG_T Cuts at: 1203 6427 7404 1203 Size: 5224 977 1327 Fragments arranged by size: 5224 1327 977 AgeI A'CCGG_T Cuts at: 6359 6359 Size: 7528 AhdI GACnn_n'nnGTC Cuts at: 6257 6257 Size: 7528 AleI CACnn'nnGTG Cuts at: 1166 3837 1166 Size: 2671 4857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7528 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2624 2656 2726 2758 2624 Size: 32 70 32 7394 Fragments arranged by size: 7394 70 32 32 AlwNI CAG_nnn'CTG Cuts at: 2737 3148 3331 3694 4282 4476 5271 6843 2737 Size: 411 183 363 588 194 795 1572 3422 Fragments arranged by size: 3422 1572 795 588 411 363 194 183 ApaI G_GGCC'C Cuts at: 2799 3910 4624 2799 Size: 1111 714 5703 Fragments arranged by size: 5703 1111 714 ApaLI G'TGCA_C Cuts at: 5585 6741 5585 Size: 1156 6372 ApoI r'AATT_y Cuts at: 1493 4837 5063 5215 7324 1493 Size: 3344 226 152 2109 1697 Fragments arranged by size: 3344 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6082 160 Size: 872 5050 1606 Fragments arranged by size: 5050 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2520 4853 1870 Size: 73 147 430 2333 4545 Fragments arranged by size: 4545 2333 430 147 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2258 2291 2415 2448 2258 Size: 33 124 33 7338 Fragments arranged by size: 7338 124 33 33 BamHI G'GATC_C Cuts at: 2111 3919 4858 5225 2111 Size: 1808 939 367 4414 Fragments arranged by size: 4414 1808 939 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3546 4389 4849 6304 618 Size: 325 217 627 1759 843 460 1455 1842 Fragments arranged by size: 1842 1759 1455 843 627 460 325 217 BanII G_rGCy'C Cuts at: 1391 2799 2921 3020 3910 4434 4624 4847 1391 Size: 1408 122 99 890 524 190 223 4072 Fragments arranged by size: 4072 1408 890 524 223 190 122 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3573 928 Size: 971 1674 4883 Fragments arranged by size: 4883 1674 971 BbvCI CC'TCA_GC Cuts at: 4175 4175 Size: 7528 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5719 5753 1903 Size: 34 3782 34 3678 Fragments arranged by size: 3782 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5420 6630 4421 Size: 999 1210 5319 Fragments arranged by size: 5319 1210 999 BclI T'GATC_A Cuts at: 1548 4648 1548 Size: 3100 4428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3732 6139 Size: 107 122 71 631 605 337 1723 2407 Cuts at: 6139 136 Size: 1525 Fragments arranged by size: 2407 1723 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2205 2346 4639 7523 2205 Size: 141 2293 2884 2210 Fragments arranged by size: 2884 2293 2210 141 BlpI GC'TnA_GC Cuts at: 2069 2710 4945 2069 Size: 641 2235 4652 Fragments arranged by size: 4652 2235 641 Bme1580I G_kGCm'C Cuts at: 1790 2799 3910 4624 4826 5589 6745 1790 Size: 1009 1111 714 202 763 1156 2573 Fragments arranged by size: 2573 1156 1111 1009 763 714 202 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7528 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3913 5259 6217 Size: 416 53 83 399 24 2496 1346 958 Cuts at: 6217 442 Size: 1753 Fragments arranged by size: 2496 1753 1346 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7528 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3034 3212 4516 4570 3034 Size: 178 1304 54 5992 Fragments arranged by size: 5992 1304 178 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 3107 3434 4756 4798 4799 6188 1239 Size: 1139 729 327 1322 42 1 1389 2579 Fragments arranged by size: 2579 1389 1322 1139 729 327 42 1 Bpu10I CC'TnA_GC Cuts at: 4175 4667 4175 Size: 492 7036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3380 3905 4214 4418 5653 6518 6816 3380 Size: 525 309 204 1235 865 298 4092 Fragments arranged by size: 4092 1235 865 525 309 298 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3183 4394 6191 882 Size: 617 1684 1211 1797 2219 Fragments arranged by size: 2219 1797 1684 1211 617 BsaAI yAC'GTr Cuts at: 493 1204 3028 3271 5319 493 Size: 711 1824 243 2048 2702 Fragments arranged by size: 2702 2048 1824 711 243 BsaBI GATnn'nnATC Cuts at: 7522 7522 Size: 7528 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3987 5335 5717 6352 Size: 53 83 186 936 2454 1348 382 635 Cuts at: 6352 275 Size: 1451 Fragments arranged by size: 2454 1451 1348 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2871 4596 5961 6359 6633 6780 7191 2871 Size: 1725 1365 398 274 147 411 3208 Fragments arranged by size: 3208 1725 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3080 3110 4626 4656 4735 Size: 30 165 30 1677 30 1516 30 79 Cuts at: 4735 4765 1178 Size: 30 3941 Fragments arranged by size: 3941 1677 1516 165 79 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3365 3431 3791 4211 4412 1186 Size: 2179 66 360 420 201 4302 Fragments arranged by size: 4302 2179 420 360 201 66 BseYI C'CCAG_C Cuts at: 3294 3722 4784 6731 7227 3294 Size: 428 1062 1947 496 3595 Fragments arranged by size: 3595 1947 1062 496 428 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2371 3317 3866 4295 4489 1163 Size: 573 635 946 549 429 194 4202 Fragments arranged by size: 4202 946 635 573 549 429 194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4978 5739 6767 1540 Size: 275 186 2977 761 1028 2301 Fragments arranged by size: 2977 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2921 3834 4434 4676 5589 5674 6745 2921 Size: 913 600 242 913 85 1071 3704 Fragments arranged by size: 3704 1071 913 913 600 242 85 BsmI GAATG_Cn' Cuts at: 3300 5039 5132 3300 Size: 1739 93 5696 Fragments arranged by size: 5696 1739 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2799 2921 3020 3834 3910 4434 4624 Size: 399 1009 122 99 814 76 524 190 Cuts at: 4624 4676 4826 4847 5589 5674 6745 1391 Size: 52 150 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 1009 814 742 524 399 190 150 122 99 85 76 52 21 BspEI T'CCGG_A Cuts at: 7191 7191 Size: 7528 BspHI T'CATG_A Cuts at: 3538 5417 7147 3538 Size: 1879 1730 3919 Fragments arranged by size: 3919 1879 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 2110 2745 3330 4105 4589 4883 844 Size: 1181 85 635 585 775 484 294 3489 Fragments arranged by size: 3489 1181 775 635 585 484 294 85 BsrBI CCG'CTC Cuts at: 1394 1823 2087 5415 1394 Size: 429 264 3328 3507 Fragments arranged by size: 3507 3328 429 264 BsrDI GCAATG_nn' Cuts at: 65 1248 2080 2826 6023 6197 65 Size: 1183 832 746 3197 174 1396 Fragments arranged by size: 3197 1396 1183 832 746 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 4110 6172 6359 1700 Size: 110 716 1584 2062 187 2869 Fragments arranged by size: 2869 2062 1584 716 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7528 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7528 BssSI C'ACGA_G Cuts at: 3911 4100 5588 6600 3911 Size: 189 1488 1012 4839 Fragments arranged by size: 4839 1488 1012 189 BstAPI GCAn_nnn'nTGC Cuts at: 794 3331 4081 794 Size: 2537 750 4241 Fragments arranged by size: 4241 2537 750 BstBI TT'CG_AA Cuts at: 6332 6332 Size: 7528 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7528 BstXI CCAn_nnnn'nTGG Cuts at: 2696 2696 Size: 7528 BstYI r'GATC_y Cuts at: 1972 2111 2205 2346 3555 3654 3867 3919 4422 Size: 139 94 141 1209 99 213 52 503 Cuts at: 4422 4639 4858 5225 5610 5627 7079 7165 7177 Size: 217 219 367 385 17 1452 86 12 Cuts at: 7177 7523 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1209 503 385 367 346 219 217 213 141 139 99 94 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2689 4138 513 Size: 552 939 685 1449 3903 Fragments arranged by size: 3903 1449 939 685 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6789 Fragments arranged by size: 6789 683 56 BtsI GCAGTG_nn' Cuts at: 2027 3056 4537 5044 5838 5858 2027 Size: 1029 1481 507 794 20 3697 Fragments arranged by size: 3697 1481 1029 794 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7528 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7493 DraI TTT'AAA Cuts at: 4832 4832 Size: 7528 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4328 6535 809 Size: 507 3012 2207 1802 Fragments arranged by size: 3012 2207 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 2751 3348 Size: 54 1425 50 275 18 639 282 597 Cuts at: 3348 4519 4975 5864 7493 8 Size: 1171 456 889 1629 43 Fragments arranged by size: 1629 1425 1171 889 639 597 456 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4975 1537 Size: 275 3163 4090 Fragments arranged by size: 4090 3163 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2357 3962 4269 5458 1353 Size: 963 41 1605 307 1189 3423 Fragments arranged by size: 3423 1605 1189 963 307 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3536 4403 6115 6489 6635 387 Size: 1064 138 1947 867 1712 374 146 1280 Fragments arranged by size: 1947 1712 1280 1064 867 374 146 138 EcoICRI GAG'CTC Cuts at: 2919 4432 2919 Size: 1513 6015 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2378 3107 3434 3572 3716 3986 Size: 393 671 75 729 327 138 144 270 Cuts at: 3986 4756 4798 4799 5591 6188 6975 7243 1239 Size: 770 42 1 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 770 729 671 597 393 327 270 268 144 138 75 42 1 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 7528 EcoO109I rG'GnC_Cy Cuts at: 2734 2796 3478 3751 4000 4150 4171 2734 Size: 62 682 273 249 150 21 6091 Fragments arranged by size: 6091 682 273 249 150 62 21 EcoRI G'AATT_C Cuts at: 4837 5215 4837 Size: 378 7150 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2742 3956 2742 Size: 1214 6314 FspI TGC'GCA Cuts at: 6034 6034 Size: 7528 HaeII r_GCGC'y Cuts at: 1678 2032 3646 4138 6675 1678 Size: 354 1614 492 2537 2531 Fragments arranged by size: 2537 2531 1614 492 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3130 3162 4735 4767 6169 Size: 32 135 32 1558 32 1573 32 1402 Cuts at: 6169 6201 6243 6275 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1573 1558 1402 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4872 5118 669 Size: 988 281 2934 246 3079 Fragments arranged by size: 3079 2934 988 281 246 HindIII A'AGCT_T Cuts at: 748 3938 748 Size: 3190 4338 HpaI GTT'AAC Cuts at: 5118 5118 Size: 7528 KpnI G_GTAC'C Cuts at: 3550 4393 4853 3550 Size: 843 460 6225 Fragments arranged by size: 6225 843 460 MfeI C'AATT_G Cuts at: 5127 5127 Size: 7528 MluI A'CGCG_T Cuts at: 7404 7404 Size: 7528 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2290 2661 2946 3237 6642 6826 1305 Size: 985 371 285 291 3405 184 2007 Fragments arranged by size: 3405 2007 985 371 291 285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 3350 4521 10 Size: 54 1425 343 639 879 1171 3017 Fragments arranged by size: 3017 1425 1171 879 639 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2694 3456 3837 4124 4610 5486 5845 Size: 648 1528 762 381 287 486 876 359 Cuts at: 5845 6004 518 Size: 159 2042 Fragments arranged by size: 2042 1528 876 762 648 486 381 359 287 159 MspA1I CmG'CkG Cuts at: 1606 2068 2117 4223 4944 5621 6769 7014 1606 Size: 462 49 2106 721 677 1148 245 2120 Fragments arranged by size: 2120 2106 1148 721 677 462 245 49 NcoI C'CATG_G Cuts at: 513 1065 2004 2689 4138 513 Size: 552 939 685 1449 3903 Fragments arranged by size: 3903 1449 939 685 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7528 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7528 NotI GC'GGCC_GC Cuts at: 4975 4975 Size: 7528 NspI r_CATG'y Cuts at: 1132 1229 4096 4886 6431 1132 Size: 97 2867 790 1545 2229 Fragments arranged by size: 2867 2229 1545 790 97 PasI CC'CwG_GG Cuts at: 1348 3862 1348 Size: 2514 5014 PciI A'CATG_T Cuts at: 6427 6427 Size: 7528 PflMI CCAn_nnn'nTGG Cuts at: 1108 3148 1108 Size: 2040 5488 PfoI T'CCnGG_A Cuts at: 2410 4029 4814 2410 Size: 1619 785 5124 Fragments arranged by size: 5124 1619 785 PmeI GTTT'AAAC Cuts at: 4832 4832 Size: 7528 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5597 5629 7110 7142 5597 Size: 32 1481 32 5983 Fragments arranged by size: 5983 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2734 3478 4150 2734 Size: 744 672 6112 Fragments arranged by size: 6112 744 672 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7528 PsiI TTA'TAA Cuts at: 5098 5098 Size: 7528 PspOMI G'GGCC_C Cuts at: 2795 3906 4620 2795 Size: 1111 714 5703 Fragments arranged by size: 5703 1111 714 PstI C_TGCA'G Cuts at: 830 2022 2742 3063 3327 3764 3900 4880 830 Size: 1192 720 321 264 437 136 980 3478 Fragments arranged by size: 3478 1192 980 720 437 321 264 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7528 PvuII CAG'CTG Cuts at: 4223 4223 Size: 7528 SacI G_AGCT'C Cuts at: 2921 4434 2921 Size: 1513 6015 SalI G'TCGA_C Cuts at: 1936 4870 1936 Size: 2934 4594 SbfI CC_TGCA'GG Cuts at: 4880 4880 Size: 7528 ScaI AGT'ACT Cuts at: 5776 7488 5776 Size: 1712 5816 SexAI A'CCwGG_T Cuts at: 4326 4326 Size: 7528 SfcI C'TryA_G Cuts at: 826 1045 2018 2228 2738 2971 3059 3199 3323 Size: 219 973 210 510 233 88 140 124 Cuts at: 3323 3760 3896 4340 4876 6011 6692 6883 826 Size: 437 136 444 536 1135 681 191 1471 Fragments arranged by size: 1471 1135 973 681 536 510 444 437 233 219 210 191 140 136 124 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7528 SmaI CCC'GGG Cuts at: 1872 2092 2522 4855 1872 Size: 220 430 2333 4545 Fragments arranged by size: 4545 2333 430 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 3884 4229 4433 5632 6533 Size: 197 926 1452 489 345 204 1199 901 Cuts at: 6533 6795 820 Size: 262 1553 Fragments arranged by size: 1553 1452 1199 926 901 489 345 262 204 197 SnaBI TAC'GTA Cuts at: 493 5319 493 Size: 4826 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7528 SphI G_CATG'C Cuts at: 1132 4886 1132 Size: 3754 3774 SspI AAT'ATT Cuts at: 5 52 2296 5452 5 Size: 47 2244 3156 2081 Fragments arranged by size: 3156 2244 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7528 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2689 4138 4488 4967 513 Size: 552 939 333 352 1449 350 479 3074 Fragments arranged by size: 3074 1449 939 552 479 352 350 333 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 4760 5555 5572 5725 5910 6355 Size: 1255 1122 2058 795 17 153 185 445 Cuts at: 6355 325 Size: 1498 Fragments arranged by size: 2058 1498 1255 1122 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5774 7486 Size: 274 80 33 51 1443 1730 2067 1712 Cuts at: 7486 96 Size: 138 Fragments arranged by size: 2067 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 7488 7520 3326 Size: 32 4130 32 3334 Fragments arranged by size: 4130 3334 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7528 XbaI T'CTAG_A Cuts at: 4864 4864 Size: 7528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3253 4514 3253 Size: 1261 6267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7528 XmaI C'CCGG_G Cuts at: 1870 2090 2520 4853 1870 Size: 220 430 2333 4545 Fragments arranged by size: 4545 2333 430 220 XmnI GAAnn'nnTTC Cuts at: 3591 3821 4067 5657 3591 Size: 230 246 1590 5462 Fragments arranged by size: 5462 1590 246 230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3988 5336 276 Size: 53 83 186 936 2454 1348 2468 Fragments arranged by size: 2468 2454 1348 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI