(Circular) (Six-base) MAPSORT of: pFN21AB9987.seq Check: 2627 from: 1 to: 6427 pFN21AB9987 6427 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2143 2282 2143 Size: 139 6288 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3618 3770 1937 Size: 1681 152 4594 Fragments arranged by size: 4594 1681 152 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 3695 4490 5874 6142 1632 Size: 1955 108 795 1384 268 1917 Fragments arranged by size: 1955 1917 1384 795 268 108 AfeI AGC'GCT Cuts at: 3377 3377 Size: 6427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 5326 6303 1203 Size: 4123 977 1327 Fragments arranged by size: 4123 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 4903 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 2089 2316 2782 2926 2991 3161 4170 5742 2089 Size: 227 466 144 65 170 1009 1572 2774 Fragments arranged by size: 2774 1572 1009 466 227 170 144 65 ApaLI G'TGCA_C Cuts at: 2772 4484 5640 2772 Size: 1712 1156 3559 Fragments arranged by size: 3559 1712 1156 ApoI r'AATT_y Cuts at: 1493 3281 3541 3736 3962 4114 6223 1493 Size: 1788 260 195 226 152 2109 1697 Fragments arranged by size: 2109 1788 1697 260 226 195 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3752 1870 Size: 73 934 875 4545 Fragments arranged by size: 4545 934 875 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 2854 3048 3748 5203 Size: 325 217 627 371 696 194 700 1455 Cuts at: 5203 618 Size: 1842 Fragments arranged by size: 1842 1455 700 696 627 371 325 217 194 BanII G_rGCy'C Cuts at: 1391 2404 3746 1391 Size: 1013 1342 4072 Fragments arranged by size: 4072 1342 1013 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2313 928 Size: 971 414 5042 Fragments arranged by size: 5042 971 414 BbvCI CC'TCA_GC Cuts at: 2013 2530 2013 Size: 517 5910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4618 4652 1903 Size: 34 50 34 2597 34 3678 Fragments arranged by size: 3678 2597 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2269 2772 4319 5529 2269 Size: 503 1547 1210 3167 Fragments arranged by size: 3167 1547 1210 503 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3598 5038 136 Size: 107 122 71 631 605 1926 1440 1525 Fragments arranged by size: 1926 1525 1440 631 605 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2776 3116 3594 4488 5644 1790 Size: 986 340 478 894 1156 2573 Fragments arranged by size: 2573 1156 986 894 478 340 BmgBI CAC'GTC Cuts at: 1318 3439 1318 Size: 2121 4306 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4158 5116 442 Size: 416 53 83 399 24 2741 958 1753 Fragments arranged by size: 2741 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2952 3171 5087 1239 Size: 1076 637 219 1916 2579 Fragments arranged by size: 2579 1916 1076 637 219 Bpu10I CC'TnA_GC Cuts at: 2013 2530 2013 Size: 517 5910 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2199 2271 4552 5417 5715 2199 Size: 72 2281 865 298 2911 Fragments arranged by size: 2911 2281 865 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3121 5090 882 Size: 617 1622 1969 2219 Fragments arranged by size: 2219 1969 1622 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 3404 6421 3404 Size: 3017 3410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2201 4860 5258 5532 5679 6090 2201 Size: 2659 398 274 147 411 2538 Fragments arranged by size: 2659 2538 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 2925 2955 1178 Size: 30 165 30 780 30 712 30 4650 Fragments arranged by size: 4650 780 712 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 3675 1186 Size: 815 1674 3938 Fragments arranged by size: 3938 1674 815 BseYI C'CCAG_C Cuts at: 2574 2634 2984 3141 3154 5630 6126 2574 Size: 60 350 157 13 2476 496 2875 Fragments arranged by size: 2875 2476 496 350 157 60 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 2936 2981 1163 Size: 573 424 776 45 4609 Fragments arranged by size: 4609 776 573 424 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2363 2776 3094 3564 4488 4573 5644 2363 Size: 413 318 470 924 85 1071 3146 Fragments arranged by size: 3146 1071 924 470 413 318 85 BsmI GAATG_Cn' Cuts at: 2043 3938 4031 2043 Size: 1895 93 4439 Fragments arranged by size: 4439 1895 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2363 2404 2776 3094 3116 3564 3594 Size: 399 573 41 372 318 22 448 30 Cuts at: 3594 3746 4488 4573 5644 1391 Size: 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 448 399 372 318 152 85 41 30 22 BspEI T'CCGG_A Cuts at: 2201 6090 2201 Size: 3889 2538 BspHI T'CATG_A Cuts at: 4316 6046 4316 Size: 1730 4697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 2257 2282 3782 844 Size: 1299 114 25 1500 3489 Fragments arranged by size: 3489 1500 1299 114 25 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4922 5096 65 Size: 1183 3674 174 1396 Fragments arranged by size: 3674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2905 5071 5258 1700 Size: 110 1095 2166 187 2869 Fragments arranged by size: 2869 2166 1095 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2926 2959 794 Size: 2132 33 4262 Fragments arranged by size: 4262 2132 33 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 2269 1184 Size: 1085 5342 BstXI CCAn_nnnn'nTGG Cuts at: 2834 2834 Size: 6427 BstYI r'GATC_y Cuts at: 1972 2602 3757 4124 4509 4526 5978 6064 6076 Size: 630 1155 367 385 17 1452 86 12 Cuts at: 6076 6422 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1155 630 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3383 3383 Size: 6427 BtgI C'CryG_G Cuts at: 513 1065 2810 3689 513 Size: 552 1745 879 3251 Fragments arranged by size: 3251 1745 879 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 3943 4737 4757 3943 Size: 794 20 5613 Fragments arranged by size: 5613 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DraIII CAC_nnn'GTG Cuts at: 2854 2959 2854 Size: 105 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2488 2863 3874 Size: 54 1425 50 275 18 658 375 1011 Cuts at: 3874 4763 6392 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1011 889 658 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2997 3569 3666 4357 1353 Size: 759 885 572 97 691 3423 Fragments arranged by size: 3423 885 759 691 572 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2952 3171 3587 3695 4490 5087 Size: 393 683 637 219 416 108 795 597 Cuts at: 5087 5874 6142 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 683 637 597 416 393 268 219 108 EcoO109I rG'GnC_Cy Cuts at: 2673 2707 3695 2673 Size: 34 988 5405 Fragments arranged by size: 5405 988 34 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 EcoRV GAT'ATC Cuts at: 3358 3358 Size: 6427 FspI TGC'GCA Cuts at: 2282 4933 2282 Size: 2651 3776 HaeII r_GCGC'y Cuts at: 1678 3379 3450 5574 1678 Size: 1701 71 2124 2531 Fragments arranged by size: 2531 2124 1701 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2770 2802 5068 Size: 32 135 32 794 32 372 32 2266 Cuts at: 5068 5100 5142 5174 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2266 794 372 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 2343 748 Size: 1595 4832 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 3321 4026 3321 Size: 705 5722 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 5541 5725 1305 Size: 1072 3164 184 2007 Fragments arranged by size: 3164 2007 1072 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 2865 10 Size: 54 1425 343 658 375 3572 Fragments arranged by size: 3572 1425 658 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2048 2690 3266 3557 4385 4744 4903 Size: 648 882 642 576 291 828 359 159 Cuts at: 4903 518 Size: 2042 Fragments arranged by size: 2042 882 828 648 642 576 359 291 159 MspA1I CmG'CkG Cuts at: 1606 2251 2471 2665 3158 3460 3624 3843 4520 Size: 645 220 194 493 302 164 219 677 Cuts at: 4520 5668 5913 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 645 493 302 245 220 219 194 164 NaeI GCC'GGC Cuts at: 2907 2907 Size: 6427 NcoI C'CATG_G Cuts at: 513 1065 2810 513 Size: 552 1745 4130 Fragments arranged by size: 4130 1745 552 NdeI CA'TA_TG Cuts at: 387 2860 387 Size: 2473 3954 NgoMIV G'CCGG_C Cuts at: 2905 2905 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2772 2807 3590 3785 5330 1132 Size: 97 1543 35 783 195 1545 2229 Fragments arranged by size: 2229 1545 1543 783 195 97 35 PasI CC'CwG_GG Cuts at: 1348 2991 1348 Size: 1643 4784 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 2991 1108 Size: 1452 431 4544 Fragments arranged by size: 4544 1452 431 PfoI T'CCnGG_A Cuts at: 2833 2833 Size: 6427 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 2707 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspXI vC'TCGA_Gb Cuts at: 2877 2877 Size: 6427 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2807 2839 2807 Size: 32 6395 PstI C_TGCA'G Cuts at: 830 2321 2368 2413 3121 3779 830 Size: 1491 47 45 708 658 3478 Fragments arranged by size: 3478 1491 708 658 47 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2665 3158 3460 3624 2665 Size: 493 302 164 5468 Fragments arranged by size: 5468 493 302 164 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SapI GCTCTTCn'nnn_ Cuts at: 3569 3569 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SexAI A'CCwGG_T Cuts at: 2850 2850 Size: 6427 SfcI C'TryA_G Cuts at: 826 1045 2317 2364 2409 3117 3619 3775 4910 Size: 219 1272 47 45 708 502 156 1135 Cuts at: 4910 5591 5782 826 Size: 681 191 1471 Fragments arranged by size: 1471 1272 1135 708 681 502 219 191 156 47 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2214 2286 2877 4531 5432 5694 Size: 197 926 271 72 591 1654 901 262 Cuts at: 5694 820 Size: 1553 Fragments arranged by size: 1654 1553 926 901 591 271 262 197 72 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 2108 4351 5 Size: 47 2056 2243 2081 Fragments arranged by size: 2243 2081 2056 47 StuI AGG'CCT Cuts at: 1517 2334 2598 2949 1517 Size: 817 264 351 4995 Fragments arranged by size: 4995 817 351 264 StyI C'CwwG_G Cuts at: 513 1065 2541 2810 3044 3105 3723 3866 513 Size: 552 1476 269 234 61 618 143 3074 Fragments arranged by size: 3074 1476 618 552 269 234 143 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2176 2194 2343 2346 4454 4471 4624 Size: 1255 596 18 149 3 2108 17 153 Cuts at: 4624 4809 5254 325 Size: 185 445 1498 Fragments arranged by size: 2108 1498 1255 596 445 185 153 149 18 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3492 4673 6385 Size: 274 80 33 51 1443 1515 1181 1712 Cuts at: 6385 96 Size: 138 Fragments arranged by size: 1712 1515 1443 1181 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2183 2215 2925 2957 6387 6419 2183 Size: 32 710 32 3430 32 2191 Fragments arranged by size: 3430 2191 710 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2808 2858 3712 2808 Size: 50 854 5523 Fragments arranged by size: 5523 854 50 XhoI C'TCGA_G Cuts at: 1943 2877 1943 Size: 934 5493 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI