(Circular) (Six-base) MAPSORT of: pFN21AB9986.seq Check: 7877 from: 1 to: 6841 pFN21AB9986 6841 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3109 4651 278 Size: 53 83 186 936 1573 1542 2468 Fragments arranged by size: 2468 1573 1542 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3109 3301 4184 1937 Size: 1172 192 883 4594 Fragments arranged by size: 4594 1172 883 192 Acc65I G'GTAC_C Cuts at: 4162 4162 Size: 6841 AclI AA'CG_TT Cuts at: 1563 4968 5341 1563 Size: 3405 373 3063 Fragments arranged by size: 3405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2972 3129 3554 3578 3733 4904 6288 Size: 731 609 157 425 24 155 1171 1384 Cuts at: 6288 6556 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1171 731 609 425 268 157 155 24 AflII C'TTAA_G Cuts at: 820 1017 3025 820 Size: 197 2008 4636 Fragments arranged by size: 4636 2008 197 AflIII A'CryG_T Cuts at: 1203 3326 5740 6717 1203 Size: 2123 2414 977 1327 Fragments arranged by size: 2414 2123 1327 977 AgeI A'CCGG_T Cuts at: 5672 5672 Size: 6841 AhdI GACnn_n'nnGTC Cuts at: 5570 5570 Size: 6841 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6841 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3798 1184 Size: 2614 4227 AlwNI CAG_nnn'CTG Cuts at: 2932 3382 3983 4584 6156 2932 Size: 450 601 601 1572 3617 Fragments arranged by size: 3617 1572 601 601 450 ApaLI G'TGCA_C Cuts at: 4898 6054 4898 Size: 1156 5685 ApoI r'AATT_y Cuts at: 1493 2228 2263 2301 2732 3070 3647 4000 4150 Size: 735 35 38 431 338 577 353 150 Cuts at: 4150 4376 4528 6637 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 735 577 431 353 338 226 152 150 38 35 AseI AT'TA_AT Cuts at: 160 1032 5395 160 Size: 872 4363 1606 Fragments arranged by size: 4363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 4166 1870 Size: 73 2223 4545 Fragments arranged by size: 4545 2223 73 BamHI G'GATC_C Cuts at: 4171 4538 4171 Size: 367 6474 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4162 5617 618 Size: 325 217 627 2375 1455 1842 Fragments arranged by size: 2375 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4160 1391 Size: 2769 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2591 3251 3581 4109 928 Size: 971 692 660 330 528 3660 Fragments arranged by size: 3660 971 692 660 528 330 BbvCI CC'TCA_GC Cuts at: 3383 3383 Size: 6841 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5032 5066 1903 Size: 34 3095 34 3678 Fragments arranged by size: 3678 3095 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3300 4733 5943 3300 Size: 1433 1210 4198 Fragments arranged by size: 4198 1433 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6841 BfrBI ATG'CAT Cuts at: 3365 3365 Size: 6841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3565 5452 136 Size: 107 122 71 631 605 1893 1887 1525 Fragments arranged by size: 1893 1887 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 6836 2067 Size: 4769 2072 BlpI GC'TnA_GC Cuts at: 2603 4258 2603 Size: 1655 5186 Bme1580I G_kGCm'C Cuts at: 1790 4902 6058 1790 Size: 3112 1156 2573 Fragments arranged by size: 3112 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2518 1318 Size: 1200 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3496 4572 5530 Size: 416 53 83 399 24 2079 1076 958 Cuts at: 5530 442 Size: 1753 Fragments arranged by size: 2079 1753 1076 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5501 1239 Size: 4262 2579 Bpu10I CC'TnA_GC Cuts at: 3383 3383 Size: 6841 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3440 3821 4966 5831 6129 3440 Size: 381 1145 865 298 4152 Fragments arranged by size: 4152 1145 865 381 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5504 882 Size: 617 4005 2219 Fragments arranged by size: 4005 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3130 4632 493 Size: 711 1926 1502 2702 Fragments arranged by size: 2702 1926 1502 711 BsaBI GATnn'nnATC Cuts at: 6835 6835 Size: 6841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3106 4648 5030 5665 Size: 53 83 186 936 1573 1542 382 635 Cuts at: 5665 275 Size: 1451 Fragments arranged by size: 1573 1542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5274 5672 5946 6093 6504 5274 Size: 398 274 147 411 5611 Fragments arranged by size: 5611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3714 3744 4125 4155 1178 Size: 30 165 30 2311 30 381 30 3864 Fragments arranged by size: 3864 2311 381 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2631 3371 3503 1186 Size: 1330 115 740 132 4524 Fragments arranged by size: 4524 1330 740 132 115 BseYI C'CCAG_C Cuts at: 3018 6044 6540 3018 Size: 3026 496 3319 Fragments arranged by size: 3319 3026 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3795 3896 1163 Size: 573 2059 101 4108 Fragments arranged by size: 4108 2059 573 101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4291 5052 6080 1540 Size: 275 186 2290 761 1028 2301 Fragments arranged by size: 2301 2290 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2856 4902 4987 6058 2856 Size: 2046 85 1071 3639 Fragments arranged by size: 3639 2046 1071 85 BsmI GAATG_Cn' Cuts at: 3870 4352 4445 3870 Size: 482 93 6266 Fragments arranged by size: 6266 482 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2856 4160 4902 4987 6058 1391 Size: 399 1066 1304 742 85 1071 2174 Fragments arranged by size: 2174 1304 1071 1066 742 399 85 BspEI T'CCGG_A Cuts at: 6504 6504 Size: 6841 BspHI T'CATG_A Cuts at: 2344 2953 4730 6460 2344 Size: 609 1777 1730 2725 Fragments arranged by size: 2725 1777 1730 609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3225 4196 844 Size: 2381 971 3489 Fragments arranged by size: 3489 2381 971 BsrBI CCG'CTC Cuts at: 1394 1823 4728 1394 Size: 429 2905 3507 Fragments arranged by size: 3507 2905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2408 5336 5510 65 Size: 1183 1160 2928 174 1396 Fragments arranged by size: 2928 1396 1183 1160 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5485 5672 1700 Size: 110 3675 187 2869 Fragments arranged by size: 3675 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3323 96 Size: 3227 3614 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 3941 4901 5913 3941 Size: 960 1012 4869 Fragments arranged by size: 4869 1012 960 BstAPI GCAn_nnn'nTGC Cuts at: 794 4004 794 Size: 3210 3631 BstBI TT'CG_AA Cuts at: 2136 5645 2136 Size: 3509 3332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6841 BstYI r'GATC_y Cuts at: 1972 2067 4171 4538 4923 4940 6392 6478 6490 Size: 95 2104 367 385 17 1452 86 12 Cuts at: 6490 6836 1972 Size: 346 1977 Fragments arranged by size: 2104 1977 1452 385 367 346 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2115 2115 Size: 6841 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5350 Fragments arranged by size: 5350 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6102 Fragments arranged by size: 6102 683 56 BtsI GCAGTG_nn' Cuts at: 4357 5151 5171 4357 Size: 794 20 6027 Fragments arranged by size: 6027 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6806 DraI TTT'AAA Cuts at: 2905 3780 4145 2905 Size: 875 365 5601 Fragments arranged by size: 5601 875 365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5848 809 Size: 507 4532 1802 Fragments arranged by size: 4532 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4288 5177 6806 Size: 54 1425 50 275 18 2458 889 1629 Cuts at: 6806 8 Size: 43 Fragments arranged by size: 2458 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4288 1537 Size: 275 2476 4090 Fragments arranged by size: 4090 2476 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3280 3285 4771 1353 Size: 1927 5 1486 3423 Fragments arranged by size: 3423 1927 1486 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5428 5802 5948 387 Size: 1064 138 3839 374 146 1280 Fragments arranged by size: 3839 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2972 3129 3554 3578 3733 4904 Size: 393 731 609 157 425 24 155 1171 Cuts at: 4904 5501 6288 6556 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1171 787 731 609 597 425 393 268 157 155 24 EcoO109I rG'GnC_Cy Cuts at: 2374 2374 Size: 6841 EcoRI G'AATT_C Cuts at: 2263 4150 4528 2263 Size: 1887 378 4576 Fragments arranged by size: 4576 1887 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2050 2637 2050 Size: 587 6254 FspI TGC'GCA Cuts at: 5347 5347 Size: 6841 HaeII r_GCGC'y Cuts at: 1678 5988 1678 Size: 4310 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 3350 3382 3712 Size: 32 135 32 905 32 841 32 330 Cuts at: 3712 3744 5482 5514 5556 5588 1373 Size: 32 1738 32 42 32 2626 Fragments arranged by size: 2626 1738 905 841 330 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2720 3302 4185 4431 669 Size: 988 281 782 582 883 246 3079 Fragments arranged by size: 3079 988 883 782 582 281 246 HindIII A'AGCT_T Cuts at: 748 2126 2674 3141 748 Size: 1378 548 467 4448 Fragments arranged by size: 4448 1378 548 467 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KpnI G_GTAC'C Cuts at: 4166 4166 Size: 6841 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2330 3127 5955 6139 1305 Size: 1025 797 2828 184 2007 Fragments arranged by size: 2828 2007 1025 797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5019 Fragments arranged by size: 5019 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2727 3780 4799 5158 5317 518 Size: 648 1561 1053 1019 359 159 2042 Fragments arranged by size: 2042 1561 1053 1019 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3379 3980 4257 4934 6082 6327 1606 Size: 1773 601 277 677 1148 245 2120 Fragments arranged by size: 2120 1773 1148 677 601 277 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5350 Fragments arranged by size: 5350 939 552 NdeI CA'TA_TG Cuts at: 387 2398 3231 3344 387 Size: 2011 833 113 3884 Fragments arranged by size: 3884 2011 833 113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6841 NotI GC'GGCC_GC Cuts at: 4288 4288 Size: 6841 NsiI A_TGCA'T Cuts at: 3367 3367 Size: 6841 NspI r_CATG'y Cuts at: 1132 1229 3224 3330 4199 5744 1132 Size: 97 1995 106 869 1545 2229 Fragments arranged by size: 2229 1995 1545 869 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6841 PciI A'CATG_T Cuts at: 3326 5740 3326 Size: 2414 4427 PflMI CCAn_nnn'nTGG Cuts at: 1108 3739 1108 Size: 2631 4210 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4910 4942 6423 6455 4910 Size: 32 1481 32 5296 Fragments arranged by size: 5296 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6841 PsiI TTA'TAA Cuts at: 2978 4085 4411 2978 Size: 1107 326 5408 Fragments arranged by size: 5408 1107 326 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2913 2945 2913 Size: 32 6809 PstI C_TGCA'G Cuts at: 830 4193 830 Size: 3363 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 PvuII CAG'CTG Cuts at: 3379 3980 3379 Size: 601 6240 SalI G'TCGA_C Cuts at: 1936 3300 4183 1936 Size: 1364 883 4594 Fragments arranged by size: 4594 1364 883 SbfI CC_TGCA'GG Cuts at: 4193 4193 Size: 6841 ScaI AGT'ACT Cuts at: 2691 3657 5089 6801 2691 Size: 966 1432 1712 2731 Fragments arranged by size: 2731 1712 1432 966 SfcI C'TryA_G Cuts at: 826 1045 4189 5324 6005 6196 826 Size: 219 3144 1135 681 191 1471 Fragments arranged by size: 3144 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 4168 1872 Size: 2296 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3025 3419 3836 4945 5846 6108 Size: 197 926 1082 394 417 1109 901 262 Cuts at: 6108 820 Size: 1553 Fragments arranged by size: 1553 1109 1082 926 901 417 394 262 197 SnaBI TAC'GTA Cuts at: 493 4632 493 Size: 4139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 4199 1132 Size: 3067 3774 SspI AAT'ATT Cuts at: 5 52 4765 5 Size: 47 4713 2081 Fragments arranged by size: 4713 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6841 StyI C'CwwG_G Cuts at: 513 1065 2004 3463 4280 513 Size: 552 939 1459 817 3074 Fragments arranged by size: 3074 1459 939 817 552 SwaI ATTT'AAAT Cuts at: 3780 3780 Size: 6841 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 4868 4885 5038 5223 5668 325 Size: 1255 957 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 957 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2689 3318 3323 Size: 274 80 33 51 1443 712 629 5 Cuts at: 3323 3655 3744 5087 6799 96 Size: 332 89 1343 1712 138 Fragments arranged by size: 1712 1443 1343 712 629 332 274 138 89 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3714 3746 6801 6833 3714 Size: 32 3055 32 3722 Fragments arranged by size: 3722 3055 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6841 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6841 XmaI C'CCGG_G Cuts at: 1870 4166 1870 Size: 2296 4545 XmnI GAAnn'nnTTC Cuts at: 2511 3239 3428 3705 4970 2511 Size: 728 189 277 1265 4382 Fragments arranged by size: 4382 1265 728 277 189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3107 4649 276 Size: 53 83 186 936 1573 1542 2468 Fragments arranged by size: 2468 1573 1542 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI