Restriction map for
pFN21AB9984
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tru9I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 301 GTCTAC GT^[AC][GT]AC
AccII 1088 CGCG CG^CG
AhdI 450 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 84 GTGCAC G^TGCAC
AocI 2124 CCTCAGG CC^T[ACGT]AGG
ApaI 35 GGGCCC GGGCC^C
ApaLI 84 GTGCAC G^TGCAC
AspEI 450 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 44 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1124 TGCGCA TGC^GCA
BbsI 10 GTCTTC GAAGAC(2/6)
BpuAI 10 GTCTTC GAAGAC(2/6)
BsaWI 1655 TCCGGT [AT]^CCGG[AT]
BsgI 539 GTGCAG GTGCAG(16/14)
BsmI 1671 GAATGC GAATGC(1/-1)
Bsp1407I 1256 TGTACA T^GTACA
BspMI 1168 ACCTGC ACCTGC(4/8)
BsrGI 1256 TGTACA T^GTACA
BstUI 1088 CGCG CG^CG
Bsu36I 2124 CCTCAGG CC^T[ACGT]AGG
Eam1105I 450 GACACTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2124 CCTCAGG CC^T[ACGT]AGG
EcoRI 2119 GAATTC G^AATTC
EcoRV 1361 GATATC GAT^ATC
FspI 1124 TGCGCA TGC^GCA
MvnI 1088 CGCG CG^CG
NdeI 2033 CATATG CA^TATG
PflFI 44 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1485 GCTCTTC GCTCTTC(1/4)
SmaI 803 CCCGGG CCC^GGG
SspBI 1256 TGTACA T^GTACA
Tth111I 44 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 460 CCATTCTTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 803 CCCGGG C^CCGGG
XmaI 803 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 1330 GATCC GGATC(4/5)
1930 GGATC
AlwNI 1117 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1891 CAGGTGCTG
BalI 506 TGGCCA TGG^CCA
2009 TGGCCA
BciVI 91 GTATCC GTATCC(6/5)
194 GTATCC
BglII 948 AGATCT A^GATCT
1558 AGATCT
BpmI 668 CTGGAG CTGGAG(16/14)
1676 CTGGAG
BstAPI 1116 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1765 GCAGACTTTGC
DraIII 830 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1998 CACTGAGTG
EarI 1486 CTCTTC CTCTTC(1/4)
140 GAAGAG
EcoNI 1351 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1884 CCTGAAACAGG
FauI 580 GCGGG CCCGC(4/6)
1089 GCGGG
Ksp632I 1486 CTCTTC CTCTTC(1/4)
140 GAAGAG
MaeII 674 ACGT A^CGT
1682 ACGT
MluNI 506 TGGCCA TGG^CCA
2009 TGGCCA
MscI 506 TGGCCA TGG^CCA
2009 TGGCCA
MslI 331 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CACCGCCGTG
PpuMI 707 AGGACCT [GA]G^G[AT]CC[CT]
806 GGGTCCC
PstI 1262 CTGCAG CTGCA^G
1457 CTGCAG
SmlI 1050 CTTGAG C^T[CT][GA]AG
1149 CTTGAG
SspI 1520 AATATT AAT^ATT
2042 AATATT
StuI 261 AGGCCT AGG^CCT
411 AGGCCT
TaqI 982 TCGA T^CGA
1787 TCGA
TthHB8I 982 TCGA T^CGA
1787 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI XhoII