(Circular) (Six-base) MAPSORT of: pFN21AB9984.seq Check: 6286 from: 1 to: 6838 pFN21AB9984 6838 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 4181 1937 Size: 370 1874 4594 Fragments arranged by size: 4594 1874 370 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 2617 4901 6285 6553 1632 Size: 788 197 2284 1384 268 1917 Fragments arranged by size: 2284 1917 1384 788 268 197 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 5737 6714 1203 Size: 4534 977 1327 Fragments arranged by size: 4534 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 2460 5567 2460 Size: 3107 3731 AleI CACnn'nnGTG Cuts at: 1166 3449 1166 Size: 2283 4555 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6838 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3296 3328 3296 Size: 32 6806 AlwNI CAG_nnn'CTG Cuts at: 3127 3901 4581 6153 3127 Size: 774 680 1572 3812 Fragments arranged by size: 3812 1572 774 680 ApaI G_GGCC'C Cuts at: 2044 2044 Size: 6838 ApaLI G'TGCA_C Cuts at: 2089 4895 6051 2089 Size: 2806 1156 2876 Fragments arranged by size: 2876 2806 1156 ApoI r'AATT_y Cuts at: 1493 2918 3515 3920 4124 4147 4373 4525 6634 Size: 1425 597 405 204 23 226 152 2109 Cuts at: 6634 1493 Size: 1697 Fragments arranged by size: 2109 1697 1425 597 405 226 204 152 23 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2105 2808 2998 3559 4163 1870 Size: 73 93 69 703 190 561 604 4545 Fragments arranged by size: 4545 703 604 561 190 93 73 69 BamHI G'GATC_C Cuts at: 4168 4535 4168 Size: 367 6471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2645 2992 3286 3663 4159 Size: 325 217 627 858 347 294 377 496 Cuts at: 4159 5614 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 858 627 496 377 347 325 294 217 BanII G_rGCy'C Cuts at: 1391 2044 2496 3509 4157 1391 Size: 653 452 1013 648 4072 Fragments arranged by size: 4072 1013 653 648 452 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2008 928 Size: 971 109 5758 Fragments arranged by size: 5758 971 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5029 5063 1903 Size: 34 3092 34 3678 Fragments arranged by size: 3678 3092 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2107 2210 4730 5940 2107 Size: 103 2520 1210 3005 Fragments arranged by size: 3005 2520 1210 103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2415 2831 3795 Size: 107 122 71 631 605 743 416 964 Cuts at: 3795 5449 136 Size: 1654 1525 Fragments arranged by size: 1654 1525 964 743 631 605 416 122 107 71 BglII A'GATC_T Cuts at: 2953 3563 6833 2953 Size: 610 3270 2958 Fragments arranged by size: 3270 2958 610 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 2044 2093 2997 3547 3621 4899 6055 1790 Size: 254 49 904 550 74 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 904 550 254 74 49 BmgBI CAC'GTC Cuts at: 1318 2680 1318 Size: 1362 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4569 5527 Size: 416 53 83 399 24 2243 909 958 Cuts at: 5527 442 Size: 1753 Fragments arranged by size: 2243 1753 958 909 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2694 3702 5498 1239 Size: 1455 1008 1796 2579 Fragments arranged by size: 2579 1796 1455 1008 Bpu10I CC'TnA_GC Cuts at: 2490 3753 2490 Size: 1263 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3076 3175 4963 5828 6126 3076 Size: 99 1788 865 298 3788 Fragments arranged by size: 3788 1788 865 298 99 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5501 882 Size: 617 4002 2219 Fragments arranged by size: 4002 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4629 493 Size: 711 3425 2702 Fragments arranged by size: 3425 2702 711 BsaBI GATnn'nnATC Cuts at: 6832 6832 Size: 6838 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4645 5027 5662 275 Size: 53 83 186 936 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3660 5271 5669 5943 6090 6501 3660 Size: 1611 398 274 147 411 3997 Fragments arranged by size: 3997 1611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2062 2092 3011 3041 3243 Size: 30 165 30 659 30 919 30 202 Cuts at: 3243 3273 1178 Size: 30 4743 Fragments arranged by size: 4743 919 659 202 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2605 3083 3169 1186 Size: 1419 478 86 4855 Fragments arranged by size: 4855 1419 478 86 BseYI C'CCAG_C Cuts at: 6041 6537 6041 Size: 496 6342 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2565 1163 Size: 573 829 5436 Fragments arranged by size: 5436 829 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2093 2702 3580 4097 4899 4984 6055 2093 Size: 609 878 517 802 85 1071 2876 Fragments arranged by size: 2876 1071 878 802 609 517 85 BsmI GAATG_Cn' Cuts at: 3682 4349 4442 3682 Size: 667 93 6078 Fragments arranged by size: 6078 667 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2658 2670 3678 2658 Size: 12 1008 5818 Fragments arranged by size: 5818 1008 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2093 2496 2702 2997 3509 3547 Size: 399 254 49 403 206 295 512 38 Cuts at: 3547 3580 3621 4097 4157 4899 4984 6055 1391 Size: 33 41 476 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 512 476 403 399 295 254 206 85 60 49 41 38 33 BspEI T'CCGG_A Cuts at: 6501 6501 Size: 6838 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3182 4193 844 Size: 2338 1011 3489 Fragments arranged by size: 3489 2338 1011 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2208 3204 3634 5333 5507 65 Size: 1183 960 996 430 1699 174 1396 Fragments arranged by size: 1699 1396 1183 996 960 430 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3261 96 Size: 3165 3673 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 3127 3776 794 Size: 2333 649 3856 Fragments arranged by size: 3856 2333 649 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6838 BstXI CCAn_nnnn'nTGG Cuts at: 2930 3149 3244 2930 Size: 219 95 6524 Fragments arranged by size: 6524 219 95 BstYI r'GATC_y Cuts at: 1972 2953 3563 3935 4168 4535 4920 4937 6389 Size: 981 610 372 233 367 385 17 1452 Cuts at: 6389 6475 6487 6833 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 981 610 385 372 367 346 233 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4130 4130 Size: 6838 BtgI C'CryG_G Cuts at: 513 1065 2346 3450 3480 3840 3858 4024 513 Size: 552 1281 1104 30 360 18 166 3327 Fragments arranged by size: 3327 1281 1104 552 360 166 30 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 3039 3172 3264 3331 4354 5148 5168 3039 Size: 133 92 67 1023 794 20 4709 Fragments arranged by size: 4709 1023 794 133 92 67 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 4142 4142 Size: 6838 DraIII CAC_nnn'GTG Cuts at: 2840 4008 2840 Size: 1168 5670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2511 4014 Size: 54 1425 50 275 18 232 449 1503 Cuts at: 4014 4285 5174 6803 8 Size: 271 889 1629 43 Fragments arranged by size: 1629 1503 1425 889 449 275 271 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2140 3497 4768 1353 Size: 787 1357 1271 3423 Fragments arranged by size: 3423 1357 1271 787 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2315 2867 5425 5799 5945 387 Size: 1064 138 726 552 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 726 552 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2617 2694 3702 4901 5498 6285 Size: 393 788 197 77 1008 1199 597 787 Cuts at: 6285 6553 1239 Size: 268 1524 Fragments arranged by size: 1524 1199 1008 788 787 597 393 268 197 77 EcoNI CCTnn'n_nnAGG Cuts at: 3360 3893 4133 3360 Size: 533 240 6065 Fragments arranged by size: 6065 533 240 EcoO109I rG'GnC_Cy Cuts at: 2040 2041 2713 2812 2040 Size: 1 672 99 6066 Fragments arranged by size: 6066 672 99 1 EcoRI G'AATT_C Cuts at: 4124 4147 4525 4124 Size: 23 378 6437 Fragments arranged by size: 6437 378 23 EcoRV GAT'ATC Cuts at: 3368 3368 Size: 6838 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3386 3976 3386 Size: 590 6248 FspI TGC'GCA Cuts at: 3131 5344 3131 Size: 2213 4625 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2427 2446 2459 2478 2702 Size: 32 135 32 855 19 13 19 224 Cuts at: 2702 2734 3548 3553 3555 3580 3585 3587 5479 Size: 32 814 5 2 25 5 2 1892 Cuts at: 5479 5511 5553 5585 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1892 855 814 224 135 42 32 32 32 32 32 25 19 19 13 5 5 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4182 4428 669 Size: 988 281 2244 246 3079 Fragments arranged by size: 3079 2244 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6838 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5952 6136 1305 Size: 4647 184 2007 Fragments arranged by size: 4647 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 4016 10 Size: 54 1425 343 681 1503 2832 Fragments arranged by size: 2832 1503 1425 681 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2340 3449 4796 5155 5314 518 Size: 648 1174 1109 1347 359 159 2042 Fragments arranged by size: 2042 1347 1174 1109 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2169 2380 2432 2507 2532 4254 4931 6079 Size: 563 211 52 75 25 1722 677 1148 Cuts at: 6079 6324 1606 Size: 245 2120 Fragments arranged by size: 2120 1722 1148 677 563 245 211 75 52 25 NcoI C'CATG_G Cuts at: 513 1065 3480 3840 4024 513 Size: 552 2415 360 184 3327 Fragments arranged by size: 3327 2415 552 360 184 NdeI CA'TA_TG Cuts at: 387 4039 387 Size: 3652 3186 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 4196 5741 1132 Size: 97 2967 1545 2229 Fragments arranged by size: 2967 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3548 1348 Size: 2200 4638 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6838 PfoI T'CCnGG_A Cuts at: 4110 4110 Size: 6838 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2713 2812 2713 Size: 99 6739 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PspOMI G'GGCC_C Cuts at: 2040 2040 Size: 6838 PstI C_TGCA'G Cuts at: 830 3271 3466 4190 830 Size: 2441 195 724 3478 Fragments arranged by size: 3478 2441 724 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 PvuII CAG'CTG Cuts at: 2169 2380 2507 2532 2169 Size: 211 127 25 6475 Fragments arranged by size: 6475 211 127 25 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SanDI GG'GwC_CC Cuts at: 2812 2812 Size: 6838 SapI GCTCTTCn'nnn_ Cuts at: 3497 3497 Size: 6838 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 2292 2448 3267 3462 4186 5321 6002 Size: 219 1247 156 819 195 724 1135 681 Cuts at: 6002 6193 826 Size: 191 1471 Fragments arranged by size: 1471 1247 1135 819 724 681 219 195 191 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 2810 4165 1872 Size: 938 1355 4545 Fragments arranged by size: 4545 1355 938 SmlI C'TyrA_G Cuts at: 820 1017 1943 3055 3154 4942 5843 6105 820 Size: 197 926 1112 99 1788 901 262 1553 Fragments arranged by size: 1788 1553 1112 926 901 262 197 99 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 4196 1132 Size: 3064 3774 SspI AAT'ATT Cuts at: 5 52 3527 4049 4762 5 Size: 47 3475 522 713 2081 Fragments arranged by size: 3475 2081 713 522 47 StuI AGG'CCT Cuts at: 1517 2268 2418 1517 Size: 751 150 5937 Fragments arranged by size: 5937 751 150 StyI C'CwwG_G Cuts at: 513 1065 3022 3480 3840 4024 4277 513 Size: 552 1957 458 360 184 253 3074 Fragments arranged by size: 3074 1957 552 458 360 253 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 4865 4882 5035 5220 5665 325 Size: 1255 750 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3261 5084 6796 Size: 274 80 33 51 1443 1284 1823 1712 Cuts at: 6796 96 Size: 138 Fragments arranged by size: 1823 1712 1443 1284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 2904 2936 3243 3275 6798 6830 2062 Size: 32 810 32 307 32 3523 32 2070 Fragments arranged by size: 3523 2070 810 307 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2052 1316 Size: 736 6102 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2472 2472 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 2808 4163 1870 Size: 938 1355 4545 Fragments arranged by size: 4545 1355 938 XmnI GAAnn'nnTTC Cuts at: 4967 4967 Size: 6838 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI