Restriction map for
pFN21AB8503
(Circular) (Six-base) MAPSORT of: pFN21AB8503.seq Check: 4475 from: 1 to: 7516
pFN21AB8503 7516 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5326 278
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 4637 4859 1937
Size: 1081 1619 222 4594
Fragments arranged by size:
4594 1619 1081 222
Acc65I G'GTAC_C
Cuts at: 4837 4837
Size: 7516
AclI AA'CG_TT
Cuts at: 1563 5643 6016 1563
Size: 4080 373 3063
Fragments arranged by size:
4080 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3087 3381 3670 3861 3915 4029 4049 5579
Size: 1455 294 289 191 54 114 20 1530
Cuts at: 5579 6963 7231 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1530 1455 1384 294 289 268 191
114 54 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7319
AflIII A'CryG_T
Cuts at: 1203 4376 6415 7392 1203
Size: 3173 2039 977 1327
Fragments arranged by size:
3173 2039 1327 977
AgeI A'CCGG_T
Cuts at: 6347 6347
Size: 7516
AhdI GACnn_n'nnGTC
Cuts at: 6245 6245
Size: 7516
AleI CACnn'nnGTG
Cuts at: 1166 4695 1166
Size: 3529 3987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7516
AlwNI CAG_nnn'CTG
Cuts at: 3569 5259 6831 3569
Size: 1690 1572 4254
Fragments arranged by size:
4254 1690 1572
ApaI G_GGCC'C
Cuts at: 4503 4503
Size: 7516
ApaLI G'TGCA_C
Cuts at: 5573 6729 5573
Size: 1156 6360
ApoI r'AATT_y
Cuts at: 1493 2084 2093 2213 2401 2693 2824 2975 3443
Size: 591 9 120 188 292 131 151 468
Cuts at: 3443 3966 4825 5051 5203 7312 1493
Size: 523 859 226 152 2109 1697
Fragments arranged by size:
2109 1697 859 591 523 468 292 226
188 152 151 131 120 9
AseI AT'TA_AT
Cuts at: 160 1032 2805 6070 160
Size: 872 1773 3265 1606
Fragments arranged by size:
3265 1773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 4841 1870
Size: 73 2898 4545
Fragments arranged by size:
4545 2898 73
BamHI G'GATC_C
Cuts at: 4549 4846 5213 4549
Size: 297 367 6852
Fragments arranged by size:
6852 367 297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4837 6292 618
Size: 325 217 627 3050 1455 1842
Fragments arranged by size:
3050 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4290 4503 4835 1391
Size: 2899 213 332 4072
Fragments arranged by size:
4072 2899 332 213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3387 4655 928
Size: 971 1488 1268 3789
Fragments arranged by size:
3789 1488 1268 971
BbvCI CC'TCA_GC
Cuts at: 2688 2688
Size: 7516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5707 5741 1903
Size: 34 3770 34 3678
Fragments arranged by size:
3770 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2724 5408 6618 2724
Size: 2684 1210 3622
Fragments arranged by size:
3622 2684 1210
BclI T'GATC_A
Cuts at: 1548 4387 1548
Size: 2839 4677
BfrBI ATG'CAT
Cuts at: 3111 4619 4747 3111
Size: 1508 128 5880
Fragments arranged by size:
5880 1508 128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6127 136
Size: 107 122 71 631 605 4455 1525
Fragments arranged by size:
4455 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7511 7511
Size: 7516
BlpI GC'TnA_GC
Cuts at: 4933 4933
Size: 7516
Bme1580I G_kGCm'C
Cuts at: 1790 4257 4503 5577 6733 1790
Size: 2467 246 1074 1156 2573
Fragments arranged by size:
2573 2467 1156 1074 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7516
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5247 6205 442
Size: 416 53 83 399 24 3830 958 1753
Fragments arranged by size:
3830 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2561 3831 3980 4447 6176 1239
Size: 1203 119 1270 149 467 1729 2579
Fragments arranged by size:
2579 1729 1270 1203 467 149 119
Bpu10I CC'TnA_GC
Cuts at: 2688 2688
Size: 7516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3345 4165 4582 4782 5641 6506 6804 3345
Size: 820 417 200 859 865 298 4057
Fragments arranged by size:
4057 865 859 820 417 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3765 6179 882
Size: 617 2266 2414 2219
Fragments arranged by size:
2414 2266 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5307 493
Size: 711 4103 2702
Fragments arranged by size:
4103 2702 711
BsaBI GATnn'nnATC
Cuts at: 7510 7510
Size: 7516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5323 5705 6340 275
Size: 53 83 186 936 3790 382 635 1451
Fragments arranged by size:
3790 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4552 5949 6347 6621 6768 7179 4552
Size: 1397 398 274 147 411 4889
Fragments arranged by size:
4889 1397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4420 4450 1178
Size: 30 165 30 3017 30 4244
Fragments arranged by size:
4244 3017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3491 1186
Size: 2305 5211
BseYI C'CCAG_C
Cuts at: 4768 6719 7215 4768
Size: 1951 496 5069
Fragments arranged by size:
5069 1951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2775 1163
Size: 573 1039 5904
Fragments arranged by size:
5904 1039 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4966 5727 6755 1540
Size: 275 186 2965 761 1028 2301
Fragments arranged by size:
2965 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3103 3405 4692 5577 5662 6733 3103
Size: 302 1287 885 85 1071 3886
Fragments arranged by size:
3886 1287 1071 885 302 85
BsmI GAATG_Cn'
Cuts at: 3727 5027 5120 3727
Size: 1300 93 6123
Fragments arranged by size:
6123 1300 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3103 3405 4257 4290 4503 4692 4835
Size: 399 1313 302 852 33 213 189 143
Cuts at: 4835 5577 5662 6733 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1313 1071 852 742 399 302 213
189 143 85 33
BspEI T'CCGG_A
Cuts at: 4552 7179 4552
Size: 2627 4889
BspHI T'CATG_A
Cuts at: 2209 5405 7135 2209
Size: 3196 1730 2590
Fragments arranged by size:
3196 2590 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4871 844
Size: 4027 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5403 1394
Size: 429 3580 3507
Fragments arranged by size:
3580 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2524 3283 3658 6011 6185 65
Size: 1183 1276 759 375 2353 174 1396
Fragments arranged by size:
2353 1396 1276 1183 759 375 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6160 6347 1700
Size: 110 4350 187 2869
Fragments arranged by size:
4350 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4379 96
Size: 4283 3233
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7516
BssSI C'ACGA_G
Cuts at: 5576 6588 5576
Size: 1012 6504
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2705 794
Size: 1911 5605
BstBI TT'CG_AA
Cuts at: 2023 3972 6320 2023
Size: 1949 2348 3219
Fragments arranged by size:
3219 2348 1949
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7516
BstYI r'GATC_y
Cuts at: 1972 4549 4846 5213 5598 5615 7067 7153 7165
Size: 2577 297 367 385 17 1452 86 12
Cuts at: 7165 7511 1972
Size: 346 1977
Fragments arranged by size:
2577 1977 1452 385 367 346 297 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2586 2586
Size: 7516
BtgI C'CryG_G
Cuts at: 513 1065 2004 4503 513
Size: 552 939 2499 3526
Fragments arranged by size:
3526 2499 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6777
Fragments arranged by size:
6777 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 2571 2960 4441 5032 5826 5846 2280
Size: 291 389 1481 591 794 20 3950
Fragments arranged by size:
3950 1481 794 591 389 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7481
DraI TTT'AAA
Cuts at: 2406 2763 4820 2406
Size: 357 2057 5102
Fragments arranged by size:
5102 2057 357
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6523 809
Size: 507 5207 1802
Fragments arranged by size:
5207 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4963 5852
Size: 54 1425 50 275 18 2077 1056 889
Cuts at: 5852 7481 8
Size: 1629 43
Fragments arranged by size:
2077 1629 1425 1056 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4963 1537
Size: 275 3151 4090
Fragments arranged by size:
4090 3151 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2038 2169 3384 4005 4226 4482 4680 5446
Size: 685 131 1215 621 221 256 198 766
Cuts at: 5446 1353
Size: 3423
Fragments arranged by size:
3423 1215 766 685 621 256 221 198
131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6103 6477 6623 387
Size: 1064 138 4514 374 146 1280
Fragments arranged by size:
4514 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2561 3087 3381 3670 3831 3861
Size: 393 810 119 526 294 289 161 30
Cuts at: 3861 3915 3980 4029 4049 4447 5579 6176 6963
Size: 54 65 49 20 398 1132 597 787
Cuts at: 6963 7231 1239
Size: 268 1524
Fragments arranged by size:
1524 1132 810 787 597 526 398 393
294 289 268 161 119 65 54 49
30 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2304 2304
Size: 7516
EcoRI G'AATT_C
Cuts at: 2213 2824 4825 5203 2213
Size: 611 2001 378 4526
Fragments arranged by size:
4526 2001 611 378
EcoRV GAT'ATC
Cuts at: 3283 4126 3283
Size: 843 6673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3092 3092
Size: 7516
FspI TGC'GCA
Cuts at: 6022 6022
Size: 7516
HaeII r_GCGC'y
Cuts at: 1678 6663 1678
Size: 4985 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3305 3337 3509 3541 4502
Size: 32 135 32 1733 32 172 32 961
Cuts at: 4502 4534 6157 6189 6231 6263 1373
Size: 32 1623 32 42 32 2626
Fragments arranged by size:
2626 1733 1623 961 172 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4569 4779 4860 5106 669
Size: 988 281 2631 210 81 246 3079
Fragments arranged by size:
3079 2631 988 281 246 210 81
HindIII A'AGCT_T
Cuts at: 748 2242 2854 4181 748
Size: 1494 612 1327 4083
Fragments arranged by size:
4083 1494 1327 612
HpaI GTT'AAC
Cuts at: 4569 4779 5106 4569
Size: 210 327 6979
Fragments arranged by size:
6979 327 210
KpnI G_GTAC'C
Cuts at: 4841 4841
Size: 7516
MfeI C'AATT_G
Cuts at: 3539 5115 3539
Size: 1576 5940
MluI A'CGCG_T
Cuts at: 7392 7392
Size: 7516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 2550 2981 6630 6814 1305
Size: 1111 134 431 3649 184 2007
Fragments arranged by size:
3649 2007 1111 431 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3909 10
Size: 54 1425 343 2077 3617
Fragments arranged by size:
3617 2077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 3417 4695 5474 5833 5992 518
Size: 648 858 1393 1278 779 359 159 2042
Fragments arranged by size:
2042 1393 1278 858 779 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3026 3695 4932 5609 6757 7002 1606
Size: 1420 669 1237 677 1148 245 2120
Fragments arranged by size:
2120 1420 1237 1148 677 669 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6025
Fragments arranged by size:
6025 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7516
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NsiI A_TGCA'T
Cuts at: 3113 4621 4749 3113
Size: 1508 128 5880
Fragments arranged by size:
5880 1508 128
NspI r_CATG'y
Cuts at: 1132 1229 3981 4380 4751 4874 6419 1132
Size: 97 2752 399 371 123 1545 2229
Fragments arranged by size:
2752 2229 1545 399 371 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7516
PciI A'CATG_T
Cuts at: 4376 6415 4376
Size: 2039 5477
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7516
PfoI T'CCnGG_A
Cuts at: 2458 3847 4395 2458
Size: 1389 548 5579
Fragments arranged by size:
5579 1389 548
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 7098 7130 5585
Size: 32 1481 32 5971
Fragments arranged by size:
5971 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4633 1613
Size: 3020 4496
PsiI TTA'TAA
Cuts at: 5086 5086
Size: 7516
PspOMI G'GGCC_C
Cuts at: 4499 4499
Size: 7516
PstI C_TGCA'G
Cuts at: 830 4324 4868 830
Size: 3494 544 3478
Fragments arranged by size:
3494 3478 544
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 3026 3695 3026
Size: 669 6847
SalI G'TCGA_C
Cuts at: 1936 4858 1936
Size: 2922 4594
SapI GCTCTTCn'nnn_
Cuts at: 4680 4680
Size: 7516
SbfI CC_TGCA'GG
Cuts at: 4868 4868
Size: 7516
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SexAI A'CCwGG_T
Cuts at: 3424 3424
Size: 7516
SfcI C'TryA_G
Cuts at: 826 1045 4118 4320 4638 4864 5999 6680 6871
Size: 219 3073 202 318 226 1135 681 191
Cuts at: 6871 826
Size: 1471
Fragments arranged by size:
3073 1471 1135 681 318 226 219 202
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7516
SmaI CCC'GGG
Cuts at: 1872 4843 1872
Size: 2971 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3360 4144 4597 4797 5620 6521
Size: 197 926 1417 784 453 200 823 901
Cuts at: 6521 6783 820
Size: 262 1553
Fragments arranged by size:
1553 1417 926 901 823 784 453 262
200 197
SnaBI TAC'GTA
Cuts at: 493 5307 493
Size: 4814 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7516
SphI G_CATG'C
Cuts at: 1132 4751 4874 1132
Size: 3619 123 3774
Fragments arranged by size:
3774 3619 123
SspI AAT'ATT
Cuts at: 5 52 3486 3637 3838 5440 5
Size: 47 3434 151 201 1602 2081
Fragments arranged by size:
3434 2081 1602 201 151 47
StuI AGG'CCT
Cuts at: 1517 2952 4076 1517
Size: 1435 1124 4957
Fragments arranged by size:
4957 1435 1124
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 4194 4293 4752 4955 513
Size: 552 939 165 2025 99 459 203 3074
Fragments arranged by size:
3074 2025 939 552 459 203 165 99
SwaI ATTT'AAAT
Cuts at: 2406 2406
Size: 7516
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4331 5543 5560 5713 5898 6343 325
Size: 1255 2751 1212 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1255 1212 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 4379 5762
Size: 274 80 33 51 1443 51 2351 1383
Cuts at: 5762 7474 96
Size: 1712 138
Fragments arranged by size:
2351 1712 1443 1383 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4420 4452 7476 7508 4420
Size: 32 3024 32 4428
Fragments arranged by size:
4428 3024 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7516
XbaI T'CTAG_A
Cuts at: 4852 4852
Size: 7516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 4136 2383
Size: 1753 5763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7516
XmaI C'CCGG_G
Cuts at: 1870 4841 1870
Size: 2971 4545
XmnI GAAnn'nnTTC
Cuts at: 5645 5645
Size: 7516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5324 276
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI