Restriction map for
pFN21AB5947
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbrPI BbsI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HindIII HpaI
KpnI MaeII MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Alw44I 648 GTGCAC G^TGCAC
ApaLI 648 GTGCAC G^TGCAC
AvaI 430 CCCGGG C^[CT]CG[GA]G
AviII 1469 TGCGCA TGC^GCA
BalI 347 TGGCCA TGG^CCA
BamHI 1280 GGATCC G^GATCC
BanI 426 GGCGCC G^G[CT][GA]CC
BbeI 426 GGCGCC GGCGC^C
BbvCI 1519 CCTCAGC CCTCAGC(-5/-2)
BglII 234 AGATCT A^GATCT
BseRI 1399 GAGGAG GAGGAG(10/8)
BsgI 205 CTGCAC GTGCAG(16/14)
BsiEI 1308 CGGCCG CG[GA][CT]^CG
BsmAI 727 GAGAC GTCTC(1/5)
BsmBI 727 GAGACG CGTCTC(1/5)
BsmI 1057 GCATTC GAATGC(1/-1)
BsoBI 430 CCCGGG C^[CT]CG[GA]G
BspDI 49 ATCGAT AT^CGAT
BspMI 1466 ACCTGC ACCTGC(4/8)
BstXI 1103 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 49 ATCGAT AT^CGAT
CpoI 497 CGGTCCG CG^G[AT]CCG
DraII 134 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 1308 CGGCCG C^GGCCG
EclXI 1308 CGGCCG C^GGCCG
Eco52I 1308 CGGCCG C^GGCCG
EcoO109I 134 GGGACCC [GA]G^G[ACGT]CC[CT]
FspI 1469 TGCGCA TGC^GCA
HincII 1005 GTTGAC GT[CT]^[GA]AC
HindII 1005 GTTGAC GT[CT]^[GA]AC
KasI 426 GGCGCC G^GCGCC
KspI 476 CCGCGG CCGC^GG
MluNI 347 TGGCCA TGG^CCA
MscI 347 TGGCCA TGG^CCA
NaeI 168 GCCGGC GCC^GGC
NarI 426 GGCGCC GG^CGCC
NgoMIV 168 GCCGGC G^CCGGC
NspI 836 GCATGT [GA]CATG^[CT]
PleI 966 GAGTC GAGTC(4/5)
PpuMI 134 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1387 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 12 CTGCAG CTGCA^G
PvuII 880 CAGCTG CAG^CTG
RsrII 497 CGGTCCG CG^G[AT]CCG
SacII 476 CCGCGG CCGC^GG
SexAI 191 ACCTGGT A^CC[AT]GGT
SfcI 12 CTGCAG C^T[GA][CT]AG
SfoI 426 GGCGCC GGC^GCC
SmaI 430 CCCGGG CCC^GGG
SmlI 982 CTCAAG C^T[CT][GA]AG
XcmI 77 CCATAGTCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 430 CCCGGG C^CCGGG
XmaI 430 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 408 CGCG CG^CG
477 CGCG
AcyI 426 GGCGCC G[GA]^CG[CT]C
847 GACGCC
AspHI 471 GAGCAC G[AT]GC[AT]^C
648 GTGCAC
BfaI 970 CTAG C^TAG
1153 CTAG
BglI 317 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
850 GCCTTCCTGGC
BsaHI 426 GGCGCC G[GA]^CG[CT]C
847 GACGCC
BsiHKAI 471 GAGCAC G[AT]GC[AT]^C
648 GTGCAC
BspHI 872 TCATGA T^CATGA
1415 TCATGA
BsrFI 168 GCCGGC [GA]^CCGG[CT]
1448 ACCGGC
BstEII 144 GGTTACC G^GT[ACGT]ACC
918 GGTCACC
BstPI 144 GGTTACC G^GT[ACGT]ACC
918 GGTCACC
BstUI 408 CGCG CG^CG
477 CGCG
Cfr10I 168 GCCGGC [GA]^CCGG[CT]
1448 ACCGGC
EarI 816 GAAGAG CTCTTC(1/4)
1066 GAAGAG
EcoO65I 144 GGTTACC G^GT[ACGT]ACC
918 GGTCACC
FauI 132 GCGGG CCCGC(4/6)
478 GCGGG
Hin1I 426 GGCGCC G[GA]^CG[CT]C
847 GACGCC
Ksp632I 816 GAAGAG CTCTTC(1/4)
1066 GAAGAG
MaeI 970 CTAG C^TAG
1153 CTAG
MseI 1211 TTAA T^TAA
1235 TTAA
MvnI 408 CGCG CG^CG
477 CGCG
RcaI 872 TCATGA T^CATGA
1415 TCATGA
SapI 816 GAAGAGC GCTCTTC(1/4)
1066 GAAGAGC
TfiI 587 GAATC G^A[AT]TC
1347 GATTC
Tru9I 1211 TTAA T^TAA
1235 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII