Restriction map for
pFN21AB5947
(Linear) (Six-base) MAPSORT of: KIBB5947.seq Check: 1295 from: 1 to: 1527
KIBB5947.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 754 844 977 1527
Size: 620 134 90 133 550
Fragments arranged by size:
620 550 134 133 90
AleI CACnn'nnGTG
Cuts at: 0 805 1527
Size: 805 722
AlwNI CAG_nnn'CTG
Cuts at: 0 306 423 897 1356 1527
Size: 306 117 474 459 171
Fragments arranged by size:
474 459 306 171 117
ApaLI G'TGCA_C
Cuts at: 0 648 1527
Size: 648 879
AvaI C'yCGr_G
Cuts at: 0 430 1527
Size: 430 1097
BamHI G'GATC_C
Cuts at: 0 1280 1527
Size: 1280 247
BanI G'GyrC_C
Cuts at: 0 426 1527
Size: 426 1101
BanII G_rGCy'C
Cuts at: 0 221 371 932 1527
Size: 221 150 561 595
Fragments arranged by size:
595 561 221 150
BbeI G_GCGC'C
Cuts at: 0 430 1527
Size: 430 1097
BbvCI CC'TCA_GC
Cuts at: 0 1520 1527
Size: 1520 7
BglI GCCn_nnn'nGGC
Cuts at: 0 323 856 1527
Size: 323 533 671
Fragments arranged by size:
671 533 323
BglII A'GATC_T
Cuts at: 0 234 1527
Size: 234 1293
Bme1580I G_kGCm'C
Cuts at: 0 103 652 800 1527
Size: 103 549 148 727
Fragments arranged by size:
727 549 148 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 385 1378 1501 1527
Size: 292 93 993 123 26
Fragments arranged by size:
993 292 123 93 26
Bpu10I CC'TnA_GC
Cuts at: 0 394 1126 1520 1527
Size: 394 732 394 7
Fragments arranged by size:
732 394 394 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1527
Size: 967 560
BsaHI Gr'CG_yC
Cuts at: 0 427 848 1527
Size: 427 421 679
Fragments arranged by size:
679 427 421
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 515 545 694 724 1527
Size: 142 30 343 30 149 30 803
Fragments arranged by size:
803 343 149 142 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1414 1527
Size: 1414 113
BseYI C'CCAG_C
Cuts at: 0 750 1527
Size: 750 777
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1527
Size: 190 1337
BsiEI CG_ry'CG
Cuts at: 0 1311 1527
Size: 1311 216
BsiHKAI G_wGCw'C
Cuts at: 0 475 652 1527
Size: 475 177 875
Fragments arranged by size:
875 475 177
BsmI GAATG_Cn'
Cuts at: 0 1057 1527
Size: 1057 470
BsmBI CGTCTCn'nnnn_
Cuts at: 0 721 1527
Size: 721 806
Bsp1286I G_dGCh'C
Cuts at: 0 103 221 371 475 652 800 932 1527
Size: 103 118 150 104 177 148 132 595
Fragments arranged by size:
595 177 150 148 132 118 104 103
BspHI T'CATG_A
Cuts at: 0 872 1415 1527
Size: 872 543 112
Fragments arranged by size:
872 543 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1475 1527
Size: 1475 52
BsrBI CCG'CTC
Cuts at: 0 281 414 747 1071 1527
Size: 281 133 333 324 456
Fragments arranged by size:
456 333 324 281 133
BsrFI r'CCGG_y
Cuts at: 0 168 1448 1527
Size: 168 1280 79
Fragments arranged by size:
1280 168 79
BstEII G'GTnAC_C
Cuts at: 0 144 918 1527
Size: 144 774 609
Fragments arranged by size:
774 609 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1527
Size: 1110 417
BstYI r'GATC_y
Cuts at: 0 234 545 1280 1527
Size: 234 311 735 247
Fragments arranged by size:
735 311 247 234
BtgI C'CryG_G
Cuts at: 0 311 476 694 1305 1527
Size: 311 165 218 611 222
Fragments arranged by size:
611 311 222 218 165
BtsI GCAGTG_nn'
Cuts at: 0 178 1479 1527
Size: 178 1301 48
Fragments arranged by size:
1301 178 48
ClaI AT'CG_AT
Cuts at: 0 50 1527
Size: 50 1477
EaeI y'GGCC_r
Cuts at: 0 347 857 1308 1496 1527
Size: 347 510 451 188 31
Fragments arranged by size:
510 451 347 188 31
EagI C'GGCC_G
Cuts at: 0 1308 1527
Size: 1308 219
EarI CTCTTCn'nnn_
Cuts at: 0 811 1061 1527
Size: 811 250 466
Fragments arranged by size:
811 466 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 155 607 1527
Size: 155 452 920
Fragments arranged by size:
920 452 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 385 620 754 844 977 1378 1501
Size: 292 93 235 134 90 133 401 123
Cuts at: 1501 1527
Size: 26
Fragments arranged by size:
401 292 235 134 133 123 93 90
26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 826 859 1165 1322 1527
Size: 826 33 306 157 205
Fragments arranged by size:
826 306 205 157 33
EcoO109I rG'GnC_Cy
Cuts at: 0 135 1527
Size: 135 1392
FspI TGC'GCA
Cuts at: 0 1471 1527
Size: 1471 56
HaeII r_GCGC'y
Cuts at: 0 430 1225 1440 1527
Size: 430 795 215 87
Fragments arranged by size:
795 430 215 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1255 1287 1527
Size: 537 32 686 32 240
Fragments arranged by size:
686 537 240 32 32
HincII GTy'rAC
Cuts at: 0 1007 1527
Size: 1007 520
KasI G'GCGC_C
Cuts at: 0 426 1527
Size: 426 1101
MscI TGG'CCA
Cuts at: 0 349 1527
Size: 349 1178
MslI CAynn'nnrTG
Cuts at: 0 52 212 805 1111 1527
Size: 52 160 593 306 416
Fragments arranged by size:
593 416 306 160 52
MspA1I CmG'CkG
Cuts at: 0 478 882 894 1527
Size: 478 404 12 633
Fragments arranged by size:
633 478 404 12
NaeI GCC'GGC
Cuts at: 0 170 1527
Size: 170 1357
NarI GG'CG_CC
Cuts at: 0 427 1527
Size: 427 1100
NgoMIV G'CCGG_C
Cuts at: 0 168 1527
Size: 168 1359
NspI r_CATG'y
Cuts at: 0 840 1527
Size: 840 687
PasI CC'CwG_GG
Cuts at: 0 722 1324 1527
Size: 722 602 203
Fragments arranged by size:
722 602 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 91 897 1214 1527
Size: 91 806 317 313
Fragments arranged by size:
806 317 313 91
PfoI T'CCnGG_A
Cuts at: 0 269 458 500 1022 1527
Size: 269 189 42 522 505
Fragments arranged by size:
522 505 269 189 42
PpuMI rG'GwC_Cy
Cuts at: 0 135 1527
Size: 135 1392
PshAI GACnn'nnGTC
Cuts at: 0 1391 1527
Size: 1391 136
PstI C_TGCA'G
Cuts at: 0 16 1527
Size: 16 1511
PvuII CAG'CTG
Cuts at: 0 882 1527
Size: 882 645
RsrII CG'GwC_CG
Cuts at: 0 498 1527
Size: 498 1029
SacII CC_GC'GG
Cuts at: 0 479 1527
Size: 479 1048
SanDI GG'GwC_CC
Cuts at: 0 135 1527
Size: 135 1392
SapI GCTCTTCn'nnn_
Cuts at: 0 811 1061 1527
Size: 811 250 466
Fragments arranged by size:
811 466 250
SexAI A'CCwGG_T
Cuts at: 0 191 1527
Size: 191 1336
SfcI C'TryA_G
Cuts at: 0 12 1527
Size: 12 1515
SfoI GGC'GCC
Cuts at: 0 428 1527
Size: 428 1099
SmaI CCC'GGG
Cuts at: 0 432 1527
Size: 432 1095
SmlI C'TyrA_G
Cuts at: 0 982 1527
Size: 982 545
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 937 1318 1527
Size: 486 451 381 209
Fragments arranged by size:
486 451 381 209
TatI w'GTAC_w
Cuts at: 0 1178 1527
Size: 1178 349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 515 547 694 726 1527
Size: 482 32 1 32 147 32 801
Fragments arranged by size:
801 482 147 32 32 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 1527
Size: 84 1443
XmaI C'CCGG_G
Cuts at: 0 430 1527
Size: 430 1097
Enzymes that do cut:
AcuI AleI AlwNI ApaLI AvaI BamHI BanI BanII
BbeI BbvCI BglI BglII Bme1580I BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstEII BstXI
BstYI BtgI BtsI ClaI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PstI PvuII RsrII SacII
SanDI SapI SexAI SfcI SfoI SmaI SmlI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI SacI SalI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI Tth111I XbaI XhoI XmnI ZraI