(Circular) (Six-base) MAPSORT of: pFN21AB5939.seq Check: 2176 from: 1 to: 6229 pFN21AB5939 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2302 2411 2945 4292 5676 5944 1632 Size: 602 68 109 534 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 602 534 268 109 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2577 5128 6105 1203 Size: 1374 2551 977 1327 Fragments arranged by size: 2551 1374 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 2497 4958 2497 Size: 2461 3768 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 3082 3972 5544 3082 Size: 890 1572 3767 Fragments arranged by size: 3767 1572 890 ApaI G_GGCC'C Cuts at: 2408 2408 Size: 6229 ApaLI G'TGCA_C Cuts at: 3314 4286 5442 3314 Size: 972 1156 4101 Fragments arranged by size: 4101 1156 972 ApoI r'AATT_y Cuts at: 1493 3407 3538 3764 3916 6025 1493 Size: 1914 131 226 152 2109 1697 Fragments arranged by size: 2109 1914 1697 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2909 3455 3554 1870 Size: 73 120 846 546 99 4545 Fragments arranged by size: 4545 846 546 120 99 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3550 5005 618 Size: 325 217 627 1763 1455 1842 Fragments arranged by size: 1842 1763 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2408 3548 1391 Size: 1017 1140 4072 Fragments arranged by size: 4072 1140 1017 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3144 3501 928 Size: 971 1245 357 3656 Fragments arranged by size: 3656 1245 971 357 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4420 4454 1903 Size: 34 50 34 2399 34 3678 Fragments arranged by size: 3678 2399 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 3502 1548 Size: 1954 4275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2408 2942 3318 4290 5446 1790 Size: 618 534 376 972 1156 2573 Fragments arranged by size: 2573 1156 972 618 534 376 BmgBI CAC'GTC Cuts at: 1318 2080 2494 2578 3142 1318 Size: 762 414 84 564 4405 Fragments arranged by size: 4405 762 564 414 84 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3103 3242 3960 Size: 416 53 83 399 24 1686 139 718 Cuts at: 3960 4918 442 Size: 958 1753 Fragments arranged by size: 1753 1686 958 718 416 399 139 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2521 2521 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2984 3065 3434 4889 1239 Size: 1745 81 369 1455 2579 Fragments arranged by size: 2579 1745 1455 369 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3317 4354 5219 5517 3317 Size: 1037 865 298 4029 Fragments arranged by size: 4029 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 3450 6223 3450 Size: 2773 3456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2253 4036 4418 Size: 53 83 186 936 525 195 1783 382 Cuts at: 4418 5053 275 Size: 635 1451 Fragments arranged by size: 1783 1451 936 635 525 382 195 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 1178 Size: 30 165 30 1164 30 4810 Fragments arranged by size: 4810 1164 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2084 2276 2981 2984 3014 3491 1186 Size: 895 3 192 705 3 30 477 3924 Fragments arranged by size: 3924 895 705 477 192 30 3 3 BseYI C'CCAG_C Cuts at: 2204 2277 5432 5928 2204 Size: 73 3155 496 2505 Fragments arranged by size: 3155 2505 496 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5656 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2946 3679 4440 5468 1540 Size: 275 186 945 733 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 733 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 4290 4375 5446 3318 Size: 972 85 1071 4101 Fragments arranged by size: 4101 1071 972 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 BsmBI CGTCTCn'nnnn_ Cuts at: 3233 3233 Size: 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2408 2942 3318 3548 4290 4375 5446 Size: 399 618 534 376 230 742 85 1071 Cuts at: 5446 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 618 534 399 376 230 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 2685 3584 844 Size: 1298 543 899 3489 Fragments arranged by size: 3489 1298 899 543 BsrBI CCG'CTC Cuts at: 1394 1823 3420 4116 1394 Size: 429 1597 696 3507 Fragments arranged by size: 3507 1597 696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 2050 1925 Size: 125 6104 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 2521 794 Size: 1727 4502 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 2662 1184 Size: 1478 4751 BstXI CCAn_nnnn'nTGG Cuts at: 2467 3298 2467 Size: 831 5398 BstYI r'GATC_y Cuts at: 1972 2859 3559 3926 4311 4328 5780 5866 5878 Size: 887 700 367 385 17 1452 86 12 Cuts at: 5878 6224 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 887 700 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 2256 3188 513 Size: 552 969 222 932 3554 Fragments arranged by size: 3554 969 932 552 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 487 Size: 683 56 2027 3463 Fragments arranged by size: 3463 2027 683 56 BtsI GCAGTG_nn' Cuts at: 2167 3199 3745 4539 4559 2167 Size: 1032 546 794 20 3837 Fragments arranged by size: 3837 1032 794 546 20 ClaI AT'CG_AT Cuts at: 1554 2541 1554 Size: 987 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3362 5236 809 Size: 507 2046 1874 1802 Fragments arranged by size: 2046 1874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2037 2164 Size: 54 1425 50 275 18 201 6 127 Cuts at: 2164 2241 2943 3424 3676 4565 6194 8 Size: 77 702 481 252 889 1629 43 Fragments arranged by size: 1629 1425 889 702 481 275 252 201 127 77 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2943 3676 1537 Size: 275 1131 733 4090 Fragments arranged by size: 4090 1131 733 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 4159 1353 Size: 1575 1231 3423 Fragments arranged by size: 3423 1575 1231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2302 2411 2945 2984 3065 3434 Size: 393 602 68 109 534 39 81 369 Cuts at: 3434 4292 4889 5676 5944 1239 Size: 858 597 787 268 1524 Fragments arranged by size: 1524 858 787 602 597 534 393 369 268 109 81 68 39 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2966 2927 Size: 39 6190 EcoO109I rG'GnC_Cy Cuts at: 3460 3460 Size: 6229 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2117 5376 1678 Size: 439 3259 2531 Fragments arranged by size: 3259 2531 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2567 2599 2745 Size: 32 135 32 911 32 52 32 146 Cuts at: 2745 2777 4870 4902 4944 4976 1373 Size: 32 2093 32 42 32 2626 Fragments arranged by size: 2626 2093 911 146 135 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 2592 5343 5527 1305 Size: 1176 111 2751 184 2007 Fragments arranged by size: 2751 2007 1176 184 111 MscI TGG'CCA Cuts at: 10 64 1489 1832 2166 2243 3426 10 Size: 54 1425 343 334 77 1183 2813 Fragments arranged by size: 2813 1425 1183 343 334 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 4187 4546 4705 518 Size: 648 3021 359 159 2042 Fragments arranged by size: 3021 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2357 2438 2704 3645 4322 5470 5715 Size: 598 153 81 266 941 677 1148 245 Cuts at: 5715 1606 Size: 2120 Fragments arranged by size: 2120 1148 941 677 598 266 245 153 81 NcoI C'CATG_G Cuts at: 513 1065 3188 513 Size: 552 2123 3554 Fragments arranged by size: 3554 2123 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 3194 1108 Size: 975 1111 4143 Fragments arranged by size: 4143 1111 975 PfoI T'CCnGG_A Cuts at: 3042 3411 3042 Size: 369 5860 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3460 3460 Size: 6229 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2404 2404 Size: 6229 PspXI vC'TCGA_Gb Cuts at: 2909 2909 Size: 6229 PstI C_TGCA'G Cuts at: 830 2443 3581 830 Size: 1613 1138 3478 Fragments arranged by size: 3478 1613 1138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2357 2438 2704 2357 Size: 81 266 5882 Fragments arranged by size: 5882 266 81 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2439 3577 4712 5393 5584 826 Size: 219 1394 1138 1135 681 191 1471 Fragments arranged by size: 1471 1394 1138 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 3332 3455 4333 5234 5496 Size: 197 926 966 423 123 878 901 262 Cuts at: 5496 820 Size: 1553 Fragments arranged by size: 1553 966 926 901 878 423 262 197 123 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2156 2244 2343 2448 2667 3188 3668 Size: 552 1091 88 99 105 219 521 480 Cuts at: 3668 513 Size: 3074 Fragments arranged by size: 3074 1091 552 521 480 219 105 99 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4256 4273 4426 4611 5056 325 Size: 1255 2676 17 153 185 445 1498 Fragments arranged by size: 2676 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2982 4475 6187 Size: 274 80 33 51 1443 1005 1493 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1493 1443 1005 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2078 1316 Size: 762 5467 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 2909 3455 1943 Size: 966 546 4717 Fragments arranged by size: 4717 966 546 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 4358 4358 Size: 6229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI