(Circular) (Six-base) MAPSORT of: pFN21AB5923.seq Check: 8720 from: 1 to: 6220 pFN21AB5923 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3377 3563 1937 Size: 1440 186 4594 Fragments arranged by size: 4594 1440 186 Acc65I G'GTAC_C Cuts at: 3416 3541 3416 Size: 125 6095 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 2906 4283 5667 5935 1632 Size: 920 354 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 920 354 268 AflII C'TTAA_G Cuts at: 820 1017 3167 820 Size: 197 2150 3873 Fragments arranged by size: 3873 2150 197 AflIII A'CryG_T Cuts at: 1203 5119 6096 1203 Size: 3916 977 1327 Fragments arranged by size: 3916 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3011 1184 Size: 1827 4393 AlwNI CAG_nnn'CTG Cuts at: 3963 5535 3963 Size: 1572 4648 ApaI G_GGCC'C Cuts at: 3412 3412 Size: 6220 ApaLI G'TGCA_C Cuts at: 2787 4277 5433 2787 Size: 1490 1156 3574 Fragments arranged by size: 3574 1490 1156 ApoI r'AATT_y Cuts at: 1493 2963 3529 3755 3907 6016 1493 Size: 1470 566 226 152 2109 1697 Fragments arranged by size: 2109 1697 1470 566 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2234 2748 2886 3117 3144 3411 Size: 73 250 41 514 138 231 27 267 Cuts at: 3411 3423 3545 1870 Size: 12 122 4545 Fragments arranged by size: 4545 514 267 250 231 138 122 73 41 27 12 AvrII C'CTAG_G Cuts at: 2423 2423 Size: 6220 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2403 2436 3215 3248 2403 Size: 33 779 33 5375 Fragments arranged by size: 5375 779 33 33 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 3416 3541 4996 618 Size: 325 217 627 317 1312 125 1455 1842 Fragments arranged by size: 1842 1455 1312 627 325 317 217 125 BanII G_rGCy'C Cuts at: 1391 2048 2141 2234 3412 3539 1391 Size: 657 93 93 1178 127 4072 Fragments arranged by size: 4072 1178 657 127 93 93 BbeI G_GCGC'C Cuts at: 2108 2108 Size: 6220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2658 2912 3475 928 Size: 971 759 254 563 3673 Fragments arranged by size: 3673 971 759 563 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2203 2237 4411 4445 1903 Size: 34 50 34 182 34 2174 34 3678 Fragments arranged by size: 3678 2174 182 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 4831 136 Size: 107 122 71 631 605 430 2729 1525 Fragments arranged by size: 2729 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 2228 3637 2228 Size: 1409 4811 Bme1580I G_kGCm'C Cuts at: 1790 2791 3412 4281 5437 1790 Size: 1001 621 869 1156 2573 Fragments arranged by size: 2573 1156 1001 869 621 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 3951 4909 Size: 416 53 83 399 24 1056 1478 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1753 1478 1056 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 2191 2332 3092 4880 1239 Size: 867 85 141 760 1788 2579 Fragments arranged by size: 2579 1788 867 760 141 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 4345 5210 5508 2996 Size: 1349 865 298 3708 Fragments arranged by size: 3708 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3150 3420 4883 882 Size: 617 1651 270 1463 2219 Fragments arranged by size: 2219 1651 1463 617 270 BsaAI yAC'GTr Cuts at: 493 1204 2824 4011 493 Size: 711 1620 1187 2702 Fragments arranged by size: 2702 1620 1187 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 2171 3439 4027 Size: 53 83 186 936 572 66 1268 588 Cuts at: 4027 4409 5044 275 Size: 382 635 1451 Fragments arranged by size: 1451 1268 936 635 588 572 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 2859 4653 5051 5325 5472 5883 2859 Size: 1794 398 274 147 411 3196 Fragments arranged by size: 3196 1794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2419 1186 Size: 1233 4987 BseYI C'CCAG_C Cuts at: 2153 3007 3048 3457 5423 5919 2153 Size: 854 41 409 1966 496 2454 Fragments arranged by size: 2454 1966 854 496 409 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 3670 4431 5459 1540 Size: 275 186 27 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 27 BsiHKAI G_wGCw'C Cuts at: 2048 2318 2791 3255 4281 4366 5437 2048 Size: 270 473 464 1026 85 1071 2831 Fragments arranged by size: 2831 1071 1026 473 464 270 85 BsmI GAATG_Cn' Cuts at: 2722 3731 3824 2722 Size: 1009 93 5118 Fragments arranged by size: 5118 1009 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3446 3446 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2141 2234 2318 2791 3255 3412 Size: 399 258 93 93 84 473 464 157 Cuts at: 3412 3539 4281 4366 5437 1391 Size: 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 464 399 258 157 127 93 93 85 84 BspEI T'CCGG_A Cuts at: 2859 5883 2859 Size: 3024 3196 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2814 3363 3575 844 Size: 1250 720 549 212 3489 Fragments arranged by size: 3489 1250 720 549 212 BsrBI CCG'CTC Cuts at: 1394 1823 2120 2160 2755 4107 1394 Size: 429 297 40 595 1352 3507 Fragments arranged by size: 3507 1352 595 429 297 40 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3044 4864 5051 1700 Size: 110 1234 1820 187 2869 Fragments arranged by size: 2869 1820 1234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 2687 4280 5292 2687 Size: 1593 1012 3615 Fragments arranged by size: 3615 1593 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 3139 1184 Size: 1955 4265 BstXI CCAn_nnnn'nTGG Cuts at: 3007 3007 Size: 6220 BstYI r'GATC_y Cuts at: 1972 3076 3550 3917 4302 4319 5771 5857 5869 Size: 1104 474 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1104 474 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3378 3378 Size: 6220 Bsu36I CC'TnA_GG Cuts at: 3219 3219 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2028 3430 513 Size: 552 963 1402 3303 Fragments arranged by size: 3303 1402 963 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2510 487 Size: 683 56 1284 4197 Fragments arranged by size: 4197 1284 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3253 3736 4530 4550 2965 Size: 288 483 794 20 4635 Fragments arranged by size: 4635 794 483 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 2041 2770 2041 Size: 729 5491 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3479 5227 809 Size: 507 2163 1748 1802 Fragments arranged by size: 2163 1802 1748 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2761 2826 Size: 54 1425 50 275 18 195 736 65 Cuts at: 2826 3126 3667 4556 6185 8 Size: 300 541 889 1629 43 Fragments arranged by size: 1629 1425 889 736 541 300 275 195 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2025 3667 1537 Size: 275 213 1642 4090 Fragments arranged by size: 4090 1642 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 4150 1353 Size: 2797 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2049 2508 4807 5181 5327 387 Size: 1064 138 460 459 2299 374 146 1280 Fragments arranged by size: 2299 1280 1064 460 459 374 146 138 EcoICRI GAG'CTC Cuts at: 2046 2046 Size: 6220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2191 2332 2552 2906 3092 4283 Size: 393 474 85 141 220 354 186 1191 Cuts at: 4283 4880 5667 5935 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1191 787 597 474 393 354 268 220 186 141 85 EcoNI CCTnn'n_nnAGG Cuts at: 2436 2436 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2467 2551 2572 2613 3408 2467 Size: 84 21 41 795 5279 Fragments arranged by size: 5279 795 84 41 21 EcoRI G'AATT_C Cuts at: 2963 3529 3907 2963 Size: 566 378 5276 Fragments arranged by size: 5276 566 378 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2058 2108 2132 2914 5367 1678 Size: 380 50 24 782 2453 2531 Fragments arranged by size: 2531 2453 782 380 50 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3461 3493 4861 4893 4935 Size: 32 135 32 1889 32 1368 32 42 Cuts at: 4935 4967 1373 Size: 32 2626 Fragments arranged by size: 2626 1889 1368 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KasI G'GCGC_C Cuts at: 2104 2104 Size: 6220 KpnI G_GTAC'C Cuts at: 3420 3545 3420 Size: 125 6095 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2381 5334 5518 1305 Size: 1076 2953 184 2007 Fragments arranged by size: 2953 2007 1076 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2763 2828 3128 10 Size: 54 1425 343 931 65 300 3102 Fragments arranged by size: 3102 1425 931 343 300 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 3137 4178 4537 4696 518 Size: 648 1713 258 1041 359 159 2042 Fragments arranged by size: 2042 1713 1041 648 359 258 159 MspA1I CmG'CkG Cuts at: 1606 2227 2926 3636 4313 5461 5706 1606 Size: 621 699 710 677 1148 245 2120 Fragments arranged by size: 2120 1148 710 699 677 621 245 NarI GG'CG_CC Cuts at: 2105 2105 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2486 3578 5123 1132 Size: 97 1257 1092 1545 2229 Fragments arranged by size: 2229 1545 1257 1092 97 PasI CC'CwG_GG Cuts at: 1348 3496 1348 Size: 2148 4072 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 2731 2770 3397 1108 Size: 1623 39 627 3931 Fragments arranged by size: 3931 1623 627 39 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2572 2613 2551 Size: 21 41 6158 Fragments arranged by size: 6158 41 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 3408 3408 Size: 6220 PspXI vC'TCGA_Gb Cuts at: 2193 2193 Size: 6220 PstI C_TGCA'G Cuts at: 830 2226 3179 3572 830 Size: 1396 953 393 3478 Fragments arranged by size: 3478 1396 953 393 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2227 2926 2227 Size: 699 5521 SacI G_AGCT'C Cuts at: 2048 2048 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SanDI GG'GwC_CC Cuts at: 2613 2613 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 2037 2553 2037 Size: 516 5704 SfcI C'TryA_G Cuts at: 826 1045 2222 3175 3568 4703 5384 5575 826 Size: 219 1177 953 393 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 953 681 393 219 191 SfoI GGC'GCC Cuts at: 2106 2106 Size: 6220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 2236 2750 2888 3413 3547 1872 Size: 364 514 138 525 134 4545 Fragments arranged by size: 4545 525 514 364 138 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 2193 2975 3167 4324 5225 5487 Size: 197 926 250 782 192 1157 901 262 Cuts at: 5487 820 Size: 1553 Fragments arranged by size: 1553 1157 926 901 782 262 250 197 192 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 2486 3578 1132 Size: 1354 1092 3774 Fragments arranged by size: 3774 1354 1092 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2423 3000 3659 513 Size: 552 1358 577 659 3074 Fragments arranged by size: 3074 1358 659 577 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 2984 4247 4264 4417 4602 5047 Size: 1255 647 757 1263 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1498 1263 1255 757 647 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3454 3454 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 2193 1943 Size: 250 5970 XmaI C'CCGG_G Cuts at: 1870 2234 2748 2886 3411 3545 1870 Size: 364 514 138 525 134 4545 Fragments arranged by size: 4545 525 514 364 138 134 XmnI GAAnn'nnTTC Cuts at: 2720 2874 4349 2720 Size: 154 1475 4591 Fragments arranged by size: 4591 1475 154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbvCI BfrBI BplI Bpu10I BsiWI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SapI SfiI SrfI SwaI