Restriction map for
pFN21AB5922
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI CpoI DraI
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1592 GGTACC G^GTACC
AcsI 1245 GAATTC [GA]^AATT[CT]
Alw44I 1569 GTGCAC G^TGCAC
AlwI 1419 GATCC GGATC(4/5)
AlwNI 1295 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1569 GTGCAC G^TGCAC
ApoI 1245 GAATTC [GA]^AATT[CT]
Asp718I 1592 GGTACC G^GTACC
AvaI 823 CTCGGG C^[CT]CG[GA]G
BbsI 328 GAAGAC GAAGAC(2/6)
BcgI 51 CGACAGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 180 CCGGG CC^[GC]GG
BglII 1033 AGATCT A^GATCT
BpmI 1082 CTGGAG CTGGAG(16/14)
BpuAI 328 GAAGAC GAAGAC(2/6)
BsaBI 967 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1087 GCATTC GAATGC(1/-1)
BsoBI 823 CTCGGG C^[CT]CG[GA]G
BspDI 121 ATCGAT AT^CGAT
BstAPI 1294 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 117 CCACATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 121 ATCGAT AT^CGAT
DraIII 1115 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 606 CGGCCG C^GGCCG
EclXI 606 CGGCCG C^GGCCG
Eco52I 606 CGGCCG C^GGCCG
EcoRI 1245 GAATTC G^AATTC
HgaI 424 GACGC GACGC(5/10)
KpnI 1592 GGTACC GGTAC^C
MaeII 1268 ACGT A^CGT
MamI 967 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 118 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 604 GCCGGC GCC^GGC
NciI 180 CCGGG CC^[GC]GG
NdeI 1373 CATATG CA^TATG
NgoMIV 604 GCCGGC G^CCGGC
PflMI 922 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1011 GAGCTC GAGCT^C
SmlI 1145 CTCAAG C^T[CT][GA]AG
SphI 1581 GCATGC GCATG^C
Tsp45I 819 GTCAC ^GT[GC]AC
Tsp509I 1246 AATT ^AATT
Van91I 922 CCAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 922 CCAAAGCATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 379 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTTTCTGTC
AspEI 379 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTTTCTGTC
AspHI 1011 GAGCTC G[AT]GC[AT]^C
1569 GTGCAC
BanII 128 GGGCTC G[GA]GC[CT]^C
1011 GAGCTC
BsaI 573 GAGACC GGTCTC(1/5)
978 GAGACC
BsiEI 522 CGACCG CG[GA][CT]^CG
606 CGGCCG
BsiHKAI 1011 GAGCTC G[AT]GC[AT]^C
1569 GTGCAC
Bsp1407I 515 TGTACA T^GTACA
1391 TGTACA
BsrFI 524 ACCGGC [GA]^CCGG[CT]
604 GCCGGC
BsrGI 515 TGTACA T^GTACA
1391 TGTACA
BsrI 1459 ACTGG ACTGG(1/-1)
1566 CCAGT
BstYI 1033 AGATCT [GA]^GATC[CT]
1418 AGATCC
Cfr10I 524 ACCGGC [GA]^CCGG[CT]
604 GCCGGC
EaeI 581 TGGCCG [CT]^GGCC[GA]
606 CGGCCG
Eam1105I 379 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACTTTCTGTC
KspI 265 CCGCGG CCGC^GG
1232 CCGCGG
MaeIII 819 GTCAC ^GT[ACGT]AC
1100 GTAAC
MflI 1033 AGATCT [GA]^GATC[CT]
1418 AGATCC
NspI 1076 ACATGC [GA]CATG^[CT]
1581 GCATGC
PleI 1463 GACTC GAGTC(4/5)
385 GAGTC
PpuMI 32 AGGTCCT [GA]G^G[AT]CC[CT]
44 GGGACCC
PstI 466 CTGCAG CTGCA^G
1586 CTGCAG
SacII 265 CCGCGG CCGC^GG
1232 CCGCGG
SfaNI 239 GCATC GCATC(5/9)
972 GCATC
SfcI 466 CTGCAG C^T[GA][CT]AG
1586 CTGCAG
SspBI 515 TGTACA T^GTACA
1391 TGTACA
XhoII 1033 AGATCT [GA]^GATC[CT]
1418 AGATCC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI TspRI
TthHB8I