Restriction map for
pFN21AB5922
(Linear) (Six-base) MAPSORT of: KIBB5922.seq Check: 7074 from: 1 to: 1599
KIBB5922.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
Acc65I G'GTAC_C
Cuts at: 0 1592 1599
Size: 1592 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1497 1599
Size: 296 1201 102
Fragments arranged by size:
1201 296 102
AhdI GACnn_n'nnGTC
Cuts at: 0 384 1485 1599
Size: 384 1101 114
Fragments arranged by size:
1101 384 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 913 1599
Size: 913 686
AlwNI CAG_nnn'CTG
Cuts at: 0 1300 1599
Size: 1300 299
ApaLI G'TGCA_C
Cuts at: 0 1569 1599
Size: 1569 30
ApoI r'AATT_y
Cuts at: 0 1245 1599
Size: 1245 354
AvaI C'yCGr_G
Cuts at: 0 823 1599
Size: 823 776
BanI G'GyrC_C
Cuts at: 0 183 408 1592 1599
Size: 183 225 1184 7
Fragments arranged by size:
1184 225 183 7
BanII G_rGCy'C
Cuts at: 0 132 1015 1599
Size: 132 883 584
Fragments arranged by size:
883 584 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 1599
Size: 335 1264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 74 1599
Size: 40 34 1525
Fragments arranged by size:
1525 40 34
BglII A'GATC_T
Cuts at: 0 1033 1599
Size: 1033 566
Bme1580I G_kGCm'C
Cuts at: 0 20 1573 1599
Size: 20 1553 26
Fragments arranged by size:
1553 26 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1599
Size: 1103 496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1599
Size: 1130 469
BsaI GGTCTCn'nnnn_
Cuts at: 0 567 972 1599
Size: 567 405 627
Fragments arranged by size:
627 567 405
BsaBI GATnn'nnATC
Cuts at: 0 971 1599
Size: 971 628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 493 523 705 735 1599
Size: 493 30 182 30 864
Fragments arranged by size:
864 493 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 249 412 1599
Size: 246 3 163 1187
Fragments arranged by size:
1187 246 163 3
BseYI C'CCAG_C
Cuts at: 0 41 548 655 989 1599
Size: 41 507 107 334 610
Fragments arranged by size:
610 507 334 107 41
BsiEI CG_ry'CG
Cuts at: 0 525 609 1599
Size: 525 84 990
Fragments arranged by size:
990 525 84
BsiHKAI G_wGCw'C
Cuts at: 0 1015 1573 1599
Size: 1015 558 26
Fragments arranged by size:
1015 558 26
BsmI GAATG_Cn'
Cuts at: 0 1087 1599
Size: 1087 512
Bsp1286I G_dGCh'C
Cuts at: 0 20 132 1015 1573 1599
Size: 20 112 883 558 26
Fragments arranged by size:
883 558 112 26 20
BsrFI r'CCGG_y
Cuts at: 0 524 604 1599
Size: 524 80 995
Fragments arranged by size:
995 524 80
BsrGI T'GTAC_A
Cuts at: 0 515 1391 1599
Size: 515 876 208
Fragments arranged by size:
876 515 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1300 1599
Size: 1300 299
BstXI CCAn_nnnn'nTGG
Cuts at: 0 124 1599
Size: 124 1475
BstYI r'GATC_y
Cuts at: 0 1033 1418 1599
Size: 1033 385 181
Fragments arranged by size:
1033 385 181
BtgI C'CryG_G
Cuts at: 0 265 413 869 1232 1599
Size: 265 148 456 363 367
Fragments arranged by size:
456 367 363 265 148
BtsI GCAGTG_nn'
Cuts at: 0 110 622 1599
Size: 110 512 977
Fragments arranged by size:
977 512 110
ClaI AT'CG_AT
Cuts at: 0 122 1599
Size: 122 1477
DraIII CAC_nnn'GTG
Cuts at: 0 1120 1599
Size: 1120 479
EaeI y'GGCC_r
Cuts at: 0 581 606 1599
Size: 581 25 993
Fragments arranged by size:
993 581 25
EagI C'GGCC_G
Cuts at: 0 606 1599
Size: 606 993
EarI CTCTTCn'nnn_
Cuts at: 0 221 272 290 398 942 1235 1253 1599
Size: 221 51 18 108 544 293 18 346
Fragments arranged by size:
544 346 293 221 108 51 18 18
EcoICRI GAG'CTC
Cuts at: 0 1013 1599
Size: 1013 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1103 1497 1599
Size: 296 807 394 102
Fragments arranged by size:
807 394 296 102
EcoO109I rG'GnC_Cy
Cuts at: 0 33 45 1150 1599
Size: 33 12 1105 449
Fragments arranged by size:
1105 449 33 12
EcoRI G'AATT_C
Cuts at: 0 1245 1599
Size: 1245 354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1321 1599
Size: 1321 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 784 816 1154 1186 1410 1442
Size: 370 32 382 32 338 32 224 32
Cuts at: 1442 1471 1503 1599
Size: 29 32 96
Fragments arranged by size:
382 370 338 224 96 32 32 32
32 32 29
KpnI G_GTAC'C
Cuts at: 0 1596 1599
Size: 1596 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1040 1599
Size: 530 510 559
Fragments arranged by size:
559 530 510
MslI CAynn'nnrTG
Cuts at: 0 122 1599
Size: 122 1477
MspA1I CmG'CkG
Cuts at: 0 267 811 1234 1599
Size: 267 544 423 365
Fragments arranged by size:
544 423 365 267
NaeI GCC'GGC
Cuts at: 0 606 1599
Size: 606 993
NdeI CA'TA_TG
Cuts at: 0 1374 1599
Size: 1374 225
NgoMIV G'CCGG_C
Cuts at: 0 604 1599
Size: 604 995
NspI r_CATG'y
Cuts at: 0 1080 1585 1599
Size: 1080 505 14
Fragments arranged by size:
1080 505 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 928 1599
Size: 928 671
PpuMI rG'GwC_Cy
Cuts at: 0 33 45 1599
Size: 33 12 1554
Fragments arranged by size:
1554 33 12
PstI C_TGCA'G
Cuts at: 0 470 1590 1599
Size: 470 1120 9
Fragments arranged by size:
1120 470 9
SacI G_AGCT'C
Cuts at: 0 1015 1599
Size: 1015 584
SacII CC_GC'GG
Cuts at: 0 268 1235 1599
Size: 268 967 364
Fragments arranged by size:
967 364 268
SanDI GG'GwC_CC
Cuts at: 0 45 1599
Size: 45 1554
SfcI C'TryA_G
Cuts at: 0 466 1586 1599
Size: 466 1120 13
Fragments arranged by size:
1120 466 13
SmlI C'TyrA_G
Cuts at: 0 1145 1599
Size: 1145 454
SphI G_CATG'C
Cuts at: 0 1585 1599
Size: 1585 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 1599
Size: 591 1008
TatI w'GTAC_w
Cuts at: 0 515 1391 1599
Size: 515 876 208
Fragments arranged by size:
876 515 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 1599
Size: 705 32 862
Fragments arranged by size:
862 705 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 929 1599
Size: 929 670
Enzymes that do cut:
Acc65I AcuI AhdI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BcgI BglII Bme1580I BpmI BpuEI
BsaI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI
ClaI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI Hin4I KpnI MmeI MslI MspA1I NaeI
NdeI NgoMIV NspI PflMI PpuMI PstI SacI SacII
SanDI SfcI SmlI SphI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaWI
BsgI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EciI EcoNI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MscI
NarI NcoI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI