(Circular) (Six-base) MAPSORT of: pFN21AB5791.seq Check: 9258 from: 1 to: 7195 pFN21AB5791 7195 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2558 3443 3803 2558 Size: 885 360 5950 Fragments arranged by size: 5950 885 360 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5005 278 Size: 53 83 186 936 3469 2468 Fragments arranged by size: 3469 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4538 1937 Size: 2601 4594 Acc65I G'GTAC_C Cuts at: 2703 3411 4516 2703 Size: 708 1105 5382 Fragments arranged by size: 5382 1105 708 AclI AA'CG_TT Cuts at: 1563 5322 5695 1563 Size: 3759 373 3063 Fragments arranged by size: 3759 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2065 2819 2857 3276 4053 4159 5258 6642 Size: 433 754 38 419 777 106 1099 1384 Cuts at: 6642 6910 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1099 777 754 433 419 268 106 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6998 AflIII A'CryG_T Cuts at: 1203 2499 2626 3608 4126 6094 7071 1203 Size: 1296 127 982 518 1968 977 1327 Fragments arranged by size: 1968 1327 1296 982 977 518 127 AgeI A'CCGG_T Cuts at: 6026 6026 Size: 7195 AhdI GACnn_n'nnGTC Cuts at: 5924 5924 Size: 7195 AleI CACnn'nnGTG Cuts at: 1166 3700 1166 Size: 2534 4661 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3557 1184 Size: 2373 4822 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 2601 Size: 32 7163 AlwNI CAG_nnn'CTG Cuts at: 2429 2983 4938 6510 2429 Size: 554 1955 1572 3114 Fragments arranged by size: 3114 1955 1572 554 ApaI G_GGCC'C Cuts at: 2721 3837 2721 Size: 1116 6079 ApaLI G'TGCA_C Cuts at: 5252 6408 5252 Size: 1156 6039 ApoI r'AATT_y Cuts at: 1493 2061 3056 3499 3776 4504 4730 4882 6991 Size: 568 995 443 277 728 226 152 2109 Cuts at: 6991 1493 Size: 1697 Fragments arranged by size: 2109 1697 995 728 568 443 277 226 152 AseI AT'TA_AT Cuts at: 160 1032 5749 160 Size: 872 4717 1606 Fragments arranged by size: 4717 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7195 AvaI C'yCGr_G Cuts at: 1870 1943 2528 2929 3375 4486 4520 1870 Size: 73 585 401 446 1111 34 4545 Fragments arranged by size: 4545 1111 585 446 401 73 34 AvrII C'CTAG_G Cuts at: 4447 4447 Size: 7195 BamHI G'GATC_C Cuts at: 2283 4525 4892 2283 Size: 2242 367 4586 Fragments arranged by size: 4586 2242 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2703 3401 3411 4516 5971 Size: 325 217 627 916 698 10 1105 1455 Cuts at: 5971 618 Size: 1842 Fragments arranged by size: 1842 1455 1105 916 698 627 325 217 10 BanII G_rGCy'C Cuts at: 1391 2619 2721 3837 4514 1391 Size: 1228 102 1116 677 4072 Fragments arranged by size: 4072 1228 1116 677 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5386 5420 1903 Size: 34 3449 34 3678 Fragments arranged by size: 3678 3449 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5087 6297 5087 Size: 1210 5985 BclI T'GATC_A Cuts at: 1548 1548 Size: 7195 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3072 5806 136 Size: 107 122 71 631 605 1400 2734 1525 Fragments arranged by size: 2734 1525 1400 631 605 122 107 71 BglII A'GATC_T Cuts at: 7190 7190 Size: 7195 BlpI GC'TnA_GC Cuts at: 3545 4612 3545 Size: 1067 6128 Bme1580I G_kGCm'C Cuts at: 1790 2089 2721 2792 3722 3837 5256 6412 1790 Size: 299 632 71 930 115 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 930 632 299 115 71 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7195 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2362 2779 2948 Size: 416 53 83 399 24 945 417 169 Cuts at: 2948 3320 3353 4926 5884 442 Size: 372 33 1573 958 1753 Fragments arranged by size: 1753 1573 958 945 417 416 399 372 169 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7195 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2543 Size: 7195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3031 3191 3495 3848 3900 4077 5855 1239 Size: 1792 160 304 353 52 177 1778 2579 Fragments arranged by size: 2579 1792 1778 353 304 177 160 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 3232 5320 6185 6483 2633 Size: 599 2088 865 298 3345 Fragments arranged by size: 3345 2088 865 599 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2525 5858 882 Size: 617 1026 3333 2219 Fragments arranged by size: 3333 2219 1026 617 BsaAI yAC'GTr Cuts at: 493 1204 2593 2629 4986 493 Size: 711 1389 36 2357 2702 Fragments arranged by size: 2702 2357 1389 711 36 BsaBI GATnn'nnATC Cuts at: 2282 2408 7189 2282 Size: 126 4781 2288 Fragments arranged by size: 4781 2288 126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5002 5384 6019 275 Size: 53 83 186 936 3469 382 635 1451 Fragments arranged by size: 3469 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2905 5628 6026 6300 6447 6858 2905 Size: 2723 398 274 147 411 3242 Fragments arranged by size: 3242 2723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2249 2279 2471 2501 2537 Size: 30 165 30 846 30 192 30 36 Cuts at: 2537 2567 2601 2631 3453 3483 1178 Size: 30 34 30 822 30 4890 Fragments arranged by size: 4890 846 822 192 165 36 34 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3288 1186 Size: 2102 5093 BseYI C'CCAG_C Cuts at: 2720 2947 6398 6894 2720 Size: 227 3451 496 3021 Fragments arranged by size: 3451 3021 496 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2677 2701 2990 3114 3517 4218 1163 Size: 573 941 24 289 124 403 701 4140 Fragments arranged by size: 4140 941 701 573 403 289 124 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3674 4645 5406 6434 1540 Size: 275 186 1673 971 761 1028 2301 Fragments arranged by size: 2301 1673 1028 971 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 2619 2634 5256 5341 6412 2451 Size: 168 15 2622 85 1071 3234 Fragments arranged by size: 3234 2622 1071 168 85 15 BsmI GAATG_Cn' Cuts at: 2738 2846 3521 3854 4706 4799 2738 Size: 108 675 333 852 93 5134 Fragments arranged by size: 5134 852 675 333 108 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2451 2619 2634 2721 2792 3722 Size: 399 299 362 168 15 87 71 930 Cuts at: 3722 3837 4514 5256 5341 6412 1391 Size: 115 677 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 677 399 362 299 168 115 87 85 71 15 BspEI T'CCGG_A Cuts at: 6858 6858 Size: 7195 BspHI T'CATG_A Cuts at: 2634 5084 6814 2634 Size: 2450 1730 3015 Fragments arranged by size: 3015 2450 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 2834 3443 3803 3896 4292 4550 844 Size: 1714 276 609 360 93 396 258 3489 Fragments arranged by size: 3489 1714 609 396 360 276 258 93 BsrBI CCG'CTC Cuts at: 1394 1823 3025 4337 5082 1394 Size: 429 1202 1312 745 3507 Fragments arranged by size: 3507 1312 1202 745 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2924 3445 3932 5690 5864 65 Size: 1183 1676 521 487 1758 174 1396 Fragments arranged by size: 1758 1676 1396 1183 521 487 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5839 6026 1700 Size: 110 4029 187 2869 Fragments arranged by size: 4029 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7195 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7195 BssSI C'ACGA_G Cuts at: 3768 5255 6267 3768 Size: 1487 1012 4696 Fragments arranged by size: 4696 1487 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3198 794 Size: 2404 4791 BstBI TT'CG_AA Cuts at: 5999 5999 Size: 7195 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7195 BstXI CCAn_nnnn'nTGG Cuts at: 2714 2728 3844 4256 2714 Size: 14 1116 412 5653 Fragments arranged by size: 5653 1116 412 14 BstYI r'GATC_y Cuts at: 1972 2283 3352 4525 4892 5277 5294 6746 6832 Size: 311 1069 1173 367 385 17 1452 86 Cuts at: 6832 6844 7190 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1173 1069 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3006 3184 513 Size: 552 939 1002 178 4524 Fragments arranged by size: 4524 1002 939 552 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4160 487 Size: 683 56 2934 3522 Fragments arranged by size: 3522 2934 683 56 BtsI GCAGTG_nn' Cuts at: 2449 2684 2689 2978 3027 3350 4711 5505 5525 Size: 235 5 289 49 323 1361 794 20 Cuts at: 5525 2449 Size: 4119 Fragments arranged by size: 4119 1361 794 323 289 235 49 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7195 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7160 DraI TTT'AAA Cuts at: 4499 4499 Size: 7195 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6202 809 Size: 507 4886 1802 Fragments arranged by size: 4886 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2208 4258 Size: 54 1425 50 275 18 177 201 2050 Cuts at: 4258 4642 5531 7160 8 Size: 384 889 1629 43 Fragments arranged by size: 2050 1629 1425 889 384 275 201 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4642 1537 Size: 275 2830 4090 Fragments arranged by size: 4090 2830 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2877 5125 1353 Size: 1524 2248 3423 Fragments arranged by size: 3423 2248 1524 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5782 6156 6302 387 Size: 1064 138 4193 374 146 1280 Fragments arranged by size: 4193 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2617 2617 Size: 7195 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2819 2857 3031 3191 3276 3495 Size: 393 433 754 38 174 160 85 219 Cuts at: 3495 3848 3900 4053 4077 4159 5258 5855 6642 Size: 353 52 153 24 82 1099 597 787 Cuts at: 6642 6910 1239 Size: 268 1524 Fragments arranged by size: 1524 1099 787 754 597 433 393 353 268 219 174 160 153 85 82 52 38 24 EcoNI CCTnn'n_nnAGG Cuts at: 2243 2811 3915 2243 Size: 568 1104 5523 Fragments arranged by size: 5523 1104 568 EcoO109I rG'GnC_Cy Cuts at: 2248 3833 2248 Size: 1585 5610 EcoRI G'AATT_C Cuts at: 3499 3776 4504 4882 3499 Size: 277 728 378 5812 Fragments arranged by size: 5812 728 378 277 FspI TGC'GCA Cuts at: 5701 5701 Size: 7195 HaeII r_GCGC'y Cuts at: 1678 6342 1678 Size: 4664 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4020 4052 5836 5868 5910 Size: 32 135 32 2448 32 1784 32 42 Cuts at: 5910 5942 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 1784 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4269 4539 4785 669 Size: 988 281 2331 270 246 3079 Fragments arranged by size: 3079 2331 988 281 270 246 HindIII A'AGCT_T Cuts at: 748 3336 748 Size: 2588 4607 HpaI GTT'AAC Cuts at: 4785 4785 Size: 7195 KpnI G_GTAC'C Cuts at: 2707 3415 4520 2707 Size: 708 1105 5382 Fragments arranged by size: 5382 1105 708 MfeI C'AATT_G Cuts at: 4794 4794 Size: 7195 MluI A'CGCG_T Cuts at: 7071 7071 Size: 7195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2881 4182 6309 6493 1305 Size: 1576 1301 2127 184 2007 Fragments arranged by size: 2127 2007 1576 1301 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2210 4260 10 Size: 54 1425 343 177 201 2050 2945 Fragments arranged by size: 2945 2050 1425 343 201 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2158 2308 3700 5153 5512 5671 518 Size: 648 992 150 1392 1453 359 159 2042 Fragments arranged by size: 2042 1453 1392 992 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 3051 3105 3169 3249 4059 4611 5288 6436 Size: 1445 54 64 80 810 552 677 1148 Cuts at: 6436 6681 1606 Size: 245 2120 Fragments arranged by size: 2120 1445 1148 810 677 552 245 80 64 54 NcoI C'CATG_G Cuts at: 513 1065 2004 3006 513 Size: 552 939 1002 4702 Fragments arranged by size: 4702 1002 939 552 NdeI CA'TA_TG Cuts at: 387 4456 387 Size: 4069 3126 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7195 NotI GC'GGCC_GC Cuts at: 4642 4642 Size: 7195 NspI r_CATG'y Cuts at: 1132 1229 2503 3100 3612 4101 4130 4248 4553 Size: 97 1274 597 512 489 29 118 305 Cuts at: 4553 6098 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1274 597 512 489 305 118 97 29 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7195 PciI A'CATG_T Cuts at: 2499 3608 4126 6094 2499 Size: 1109 518 1968 3600 Fragments arranged by size: 3600 1968 1109 518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2653 4164 1108 Size: 942 603 1511 4139 Fragments arranged by size: 4139 1511 942 603 PfoI T'CCnGG_A Cuts at: 3979 4308 3979 Size: 329 6866 PmeI GTTT'AAAC Cuts at: 4499 4499 Size: 7195 PmlI CAC'GTG Cuts at: 2629 2629 Size: 7195 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2601 2633 5264 5296 6777 6809 2601 Size: 32 2631 32 1481 32 2987 Fragments arranged by size: 2987 2631 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2248 Size: 7195 PshAI GACnn'nnGTC Cuts at: 1613 3124 4488 1613 Size: 1511 1364 4320 Fragments arranged by size: 4320 1511 1364 PsiI TTA'TAA Cuts at: 3600 4765 3600 Size: 1165 6030 PspOMI G'GGCC_C Cuts at: 2717 3833 2717 Size: 1116 6079 PstI C_TGCA'G Cuts at: 830 2510 2645 2870 3428 4064 4547 830 Size: 1680 135 225 558 636 483 3478 Fragments arranged by size: 3478 1680 636 558 483 225 135 PvuI CG_AT'CG Cuts at: 2001 3674 2001 Size: 1673 5522 PvuII CAG'CTG Cuts at: 3051 4059 3051 Size: 1008 6187 SacI G_AGCT'C Cuts at: 2619 2619 Size: 7195 SalI G'TCGA_C Cuts at: 1936 4537 1936 Size: 2601 4594 SbfI CC_TGCA'GG Cuts at: 4547 4547 Size: 7195 ScaI AGT'ACT Cuts at: 5443 7155 5443 Size: 1712 5483 SfcI C'TryA_G Cuts at: 826 1045 2260 2506 2641 2866 3076 3238 3424 Size: 219 1215 246 135 225 210 162 186 Cuts at: 3424 3473 3967 4060 4543 5678 6359 6550 826 Size: 49 494 93 483 1135 681 191 1471 Fragments arranged by size: 1471 1215 1135 681 494 483 246 225 219 210 191 186 162 135 93 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7195 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7195 SmaI CCC'GGG Cuts at: 1872 3377 4522 1872 Size: 1505 1145 4545 Fragments arranged by size: 4545 1505 1145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 2612 3211 5299 6200 6462 Size: 197 926 585 84 599 2088 901 262 Cuts at: 6462 820 Size: 1553 Fragments arranged by size: 2088 1553 926 901 599 585 262 197 84 SnaBI TAC'GTA Cuts at: 493 4986 493 Size: 4493 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7195 SphI G_CATG'C Cuts at: 1132 4101 4248 4553 1132 Size: 2969 147 305 3774 Fragments arranged by size: 3774 2969 305 147 SspI AAT'ATT Cuts at: 5 52 2167 5119 5 Size: 47 2115 2952 2081 Fragments arranged by size: 2952 2115 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7195 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 4447 4634 513 Size: 552 939 1002 1441 187 3074 Fragments arranged by size: 3074 1441 1002 939 552 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 4355 5222 5239 5392 5577 6022 Size: 1255 722 2053 867 17 153 185 445 Cuts at: 6022 325 Size: 1498 Fragments arranged by size: 2053 1498 1255 867 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3665 3963 5441 Size: 274 80 33 51 1443 1688 298 1478 Cuts at: 5441 7153 96 Size: 1712 138 Fragments arranged by size: 1712 1688 1478 1443 298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2895 2927 3453 3485 7155 7187 2895 Size: 32 526 32 3670 32 2903 Fragments arranged by size: 3670 2903 526 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7195 XbaI T'CTAG_A Cuts at: 4207 4531 4207 Size: 324 6871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4223 4223 Size: 7195 XhoI C'TCGA_G Cuts at: 1943 2528 1943 Size: 585 6610 XmaI C'CCGG_G Cuts at: 1870 3375 4520 1870 Size: 1505 1145 4545 Fragments arranged by size: 4545 1505 1145 XmnI GAAnn'nnTTC Cuts at: 5324 5324 Size: 7195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5003 276 Size: 53 83 186 936 3469 2468 Fragments arranged by size: 3469 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI