Restriction map for
pFN21AB5913
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AclI AcsI AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BclI BglII BlnI
BpuAI BsaAI BsaBI BseRI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 535 GACGTC GACGT^C
AccI 448 GTCTAC GT^[AC][GT]AC
AccII 810 CGCG CG^CG
AccIII 1322 TCCGGA T^CCGGA
AcyI 535 GACGTC G[GA]^CG[CT]C
AflII 1417 CTTAAG C^TTAAG
AflIII 527 ACATGT A^C[GA][CT]GT
AocI 1452 CCTCAGG CC^T[ACGT]AGG
AspI 814 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 387 GGATCC G^GATCC
BanI 670 GGCACC G^G[CT][GA]CC
BciVI 1207 GTATCC GTATCC(6/5)
BfrI 1417 CTTAAG C^TTAAG
BsaHI 535 GACGTC G[GA]^CG[CT]C
BsaI 1241 GAGACC GGTCTC(1/5)
BseAI 1322 TCCGGA T^CCGGA
BsiEI 474 CGACCG CG[GA][CT]^CG
BsmAI 1241 GAGAC GTCTC(1/5)
BsmFI 429 GTCCC GGGAC(10/14)
BsmI 1068 GAATGC GAATGC(1/-1)
BspEI 1322 TCCGGA T^CCGGA
BspLU11I 527 ACATGT A^CATGT
BsrBI 1189 GAGCGG CCGCTC(-3/-3)
BstAPI 628 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 810 CGCG CG^CG
Bsu36I 1452 CCTCAGG CC^T[ACGT]AGG
DraIII 1138 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1452 CCTCAGG CC^T[ACGT]AGG
EcoRV 546 GATATC GAT^ATC
HgaI 1440 GCGTC GACGC(5/10)
Hin1I 535 GACGTC G[GA]^CG[CT]C
HincII 1354 GTCAAC GT[CT]^[GA]AC
HindII 1354 GTCAAC GT[CT]^[GA]AC
MaeIII 820 GTCAC ^GT[ACGT]AC
MroI 1322 TCCGGA T^CCGGA
MvnI 810 CGCG CG^CG
NcoI 1079 CCATGG C^CATGG
NspI 527 ACATGT [GA]CATG^[CT]
PflFI 814 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1374 GAAGAGC GCTCTTC(1/4)
SexAI 1139 ACCTGGT A^CC[AT]GGT
SfaNI 1087 GATGC GCATC(5/9)
SmlI 1417 CTTAAG C^T[CT][GA]AG
StuI 1022 AGGCCT AGG^CCT
Tsp45I 820 GTCAC ^GT[GC]AC
Tsp509I 342 AATT ^AATT
Tth111I 814 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 96 CCAGACTCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
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ApaI 975 GGGCCC GGGCC^C
1280 GGGCCC
AspHI 214 GAGCTC G[AT]GC[AT]^C
1178 GAGCTC
AvaI 1073 CTCGGG C^[CT]CG[GA]G
1181 CTCGGG
BalI 65 TGGCCA TGG^CCA
1445 TGGCCA
BfaI 1096 CTAG C^TAG
1198 CTAG
BglI 67 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
436 GCCAACCTGGC
BlpI 76 GCTCAGC GC^T[ACGT]AGC
1186 GCTGAGC
Bpu1102I 76 GCTCAGC GC^T[ACGT]AGC
1186 GCTGAGC
BsgI 392 CTGCAC GTGCAG(16/14)
1026 CTGCAC
BsiHKAI 214 GAGCTC G[AT]GC[AT]^C
1178 GAGCTC
BsoBI 1073 CTCGGG C^[CT]CG[GA]G
1181 CTCGGG
BsrDI 219 CATTGC GCAATG(2/0)
993 CATTGC
BstXI 759 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1163 CCAAGGGCCTGG
BstYI 387 GGATCC [GA]^GATC[CT]
917 AGATCC
CelII 76 GCTCAGC GC^T[ACGT]AGC
1186 GCTGAGC
EarI 842 CTCTTC CTCTTC(1/4)
1374 GAAGAG
EcoNI 1014 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1413 CCTGCTTAAGG
FauI 704 CCCGC CCCGC(4/6)
1191 GCGGG
HaeII 359 AGCGCC [GA]GCGC^[CT]
676 AGCGCC
Ksp632I 842 CTCTTC CTCTTC(1/4)
1374 GAAGAG
MaeI 1096 CTAG C^TAG
1198 CTAG
MaeII 536 ACGT A^CGT
556 ACGT
MflI 387 GGATCC [GA]^GATC[CT]
917 AGATCC
MluNI 65 TGGCCA TGG^CCA
1445 TGGCCA
MscI 65 TGGCCA TGG^CCA
1445 TGGCCA
MseI 1291 TTAA T^TAA
1418 TTAA
MspA1I 52 CAGCTG C[AC]G^C[GT]G
622 CAGCTG
PpuMI 265 GGGTCCT [GA]G^G[AT]CC[CT]
427 GGGTCCC
PvuII 52 CAGCTG CAG^CTG
622 CAGCTG
SacI 214 GAGCTC GAGCT^C
1178 GAGCTC
SbfI 966 CCTGCAGG CCTGCA^GG
1053 CCTGCAGG
ScaI 284 AGTACT AGT^ACT
1046 AGTACT
Sse8387I 966 CCTGCAGG CCTGCA^GG
1053 CCTGCAGG
TfiI 789 GATTC G^A[AT]TC
941 GAATC
Tru9I 1291 TTAA T^TAA
1418 TTAA
XhoII 387 GGATCC [GA]^GATC[CT]
917 AGATCC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI TspRI TthHB8I