(Circular) (Six-base) MAPSORT of: pFN21AB5913.seq Check: 4566 from: 1 to: 6217 pFN21AB5913 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2754 2754 Size: 6217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2544 4027 278 Size: 53 83 186 936 1008 1483 2468 Fragments arranged by size: 2468 1483 1008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3560 1937 Size: 517 1106 4594 Fragments arranged by size: 4594 1106 517 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3089 4280 5664 5932 1632 Size: 1457 1191 1384 268 1917 Fragments arranged by size: 1917 1457 1384 1191 268 AflII C'TTAA_G Cuts at: 820 1017 3422 820 Size: 197 2405 3615 Fragments arranged by size: 3615 2405 197 AflIII A'CryG_T Cuts at: 1203 2532 5116 6093 1203 Size: 1329 2584 977 1327 Fragments arranged by size: 2584 1329 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 2769 1166 Size: 1603 4614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2130 3011 3067 3448 3960 5532 2130 Size: 881 56 381 512 1572 2815 Fragments arranged by size: 2815 1572 881 512 381 56 ApaI G_GGCC'C Cuts at: 2984 3289 2984 Size: 305 5912 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3078 3186 3542 1870 Size: 73 1135 108 356 4545 Fragments arranged by size: 4545 1135 356 108 73 BamHI G'GATC_C Cuts at: 2392 3547 3914 2392 Size: 1155 367 4695 Fragments arranged by size: 4695 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3538 4993 618 Size: 325 217 627 888 863 1455 1842 Fragments arranged by size: 1842 1455 888 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2223 2871 2984 3187 3289 3536 1391 Size: 692 140 648 113 203 102 247 4072 Fragments arranged by size: 4072 692 648 247 203 140 113 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4408 4442 1903 Size: 34 50 34 2387 34 3678 Fragments arranged by size: 3678 2387 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3223 4109 5319 3223 Size: 886 1210 4121 Fragments arranged by size: 4121 1210 886 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2447 4828 Size: 107 122 71 631 605 406 369 2381 Cuts at: 4828 136 Size: 1525 Fragments arranged by size: 2381 1525 631 605 406 369 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2082 3192 3634 2082 Size: 1110 442 4665 Fragments arranged by size: 4665 1110 442 Bme1580I G_kGCm'C Cuts at: 1790 2984 3289 4278 5434 1790 Size: 1194 305 989 1156 2573 Fragments arranged by size: 2573 1194 1156 989 305 BmgBI CAC'GTC Cuts at: 1318 2562 1318 Size: 1244 4973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2121 3948 4906 Size: 416 53 83 399 24 704 1827 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1827 1753 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2279 3264 3383 4877 1239 Size: 998 42 985 119 1494 2579 Fragments arranged by size: 2579 1494 998 985 119 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3240 4880 882 Size: 617 1741 1640 2219 Fragments arranged by size: 2219 1741 1640 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2541 4024 4406 5041 Size: 53 83 186 936 1008 1483 382 635 Cuts at: 5041 275 Size: 1451 Fragments arranged by size: 1483 1451 1008 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2481 2880 3327 4650 5048 5322 5469 5880 2481 Size: 399 447 1323 398 274 147 411 2818 Fragments arranged by size: 2818 1323 447 411 399 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2319 2349 2400 Size: 30 165 30 732 30 154 30 51 Cuts at: 2400 2430 1178 Size: 30 4965 Fragments arranged by size: 4965 732 165 154 51 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 2437 3289 5420 5916 2437 Size: 852 2131 496 2738 Fragments arranged by size: 2738 2131 852 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2382 3016 1163 Size: 573 646 634 4364 Fragments arranged by size: 4364 646 634 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2482 3667 4428 5456 1540 Size: 275 186 481 1185 761 1028 2301 Fragments arranged by size: 2301 1185 1028 761 481 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 3187 4278 4363 5434 2223 Size: 964 1091 85 1071 3006 Fragments arranged by size: 3006 1091 1071 964 85 BsmI GAATG_Cn' Cuts at: 3079 3728 3821 3079 Size: 649 93 5475 Fragments arranged by size: 5475 649 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2223 2871 2984 3187 3289 3536 Size: 399 293 140 648 113 203 102 247 Cuts at: 3536 4278 4363 5434 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 648 399 293 247 203 140 113 102 85 BspEI T'CCGG_A Cuts at: 3327 5880 3327 Size: 2553 3664 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 2754 3052 3168 3572 844 Size: 1664 246 298 116 404 3489 Fragments arranged by size: 3489 1664 404 298 246 116 BsrBI CCG'CTC Cuts at: 1394 1823 3196 4104 1394 Size: 429 1373 908 3507 Fragments arranged by size: 3507 1373 908 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2223 2997 4712 4886 65 Size: 1183 975 774 1715 174 1396 Fragments arranged by size: 1715 1396 1183 975 774 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4861 5048 1700 Size: 110 3051 187 2869 Fragments arranged by size: 3051 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2639 794 Size: 1845 4372 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2771 3175 2771 Size: 404 5813 BstYI r'GATC_y Cuts at: 1972 2392 2922 3547 3914 4299 4316 5768 5854 Size: 420 530 625 367 385 17 1452 86 Cuts at: 5854 5866 6212 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 625 530 420 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3458 3458 Size: 6217 BtgI C'CryG_G Cuts at: 513 1065 2524 2657 3084 513 Size: 552 1459 133 427 3646 Fragments arranged by size: 3646 1459 552 427 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2210 3219 3733 4527 4547 2210 Size: 1009 514 794 20 3880 Fragments arranged by size: 3880 1009 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DraIII CAC_nnn'GTG Cuts at: 3148 3148 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2448 2654 Size: 54 1425 50 275 18 240 378 206 Cuts at: 2654 2914 3450 3664 4553 6182 8 Size: 260 536 214 889 1629 43 Fragments arranged by size: 1629 1425 889 536 378 275 260 240 214 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2853 3374 4147 1353 Size: 1500 521 773 3423 Fragments arranged by size: 3423 1500 773 521 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 3185 2221 Size: 964 5253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2279 3089 3264 3383 4280 4877 Size: 393 605 42 810 175 119 897 597 Cuts at: 4877 5664 5932 1239 Size: 787 268 1524 Fragments arranged by size: 1524 897 810 787 605 597 393 268 175 119 42 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3422 3023 Size: 399 5818 EcoO109I rG'GnC_Cy Cuts at: 2263 2271 2433 2980 2981 3172 3285 3286 3486 Size: 8 162 547 1 191 113 1 200 Cuts at: 3486 2263 Size: 4994 Fragments arranged by size: 4994 547 200 191 162 113 8 1 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 EcoRV GAT'ATC Cuts at: 2553 2553 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2368 2685 5364 1678 Size: 690 317 2679 2531 Fragments arranged by size: 2679 2531 690 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2400 2432 3197 Size: 32 135 32 563 32 233 32 765 Cuts at: 3197 3229 3395 3427 4858 4890 4932 4964 1373 Size: 32 166 32 1431 32 42 32 2626 Fragments arranged by size: 2626 1431 765 563 233 166 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 3561 3807 669 Size: 988 281 1423 200 246 3079 Fragments arranged by size: 3079 1423 988 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 5331 5515 1305 Size: 828 3198 184 2007 Fragments arranged by size: 3198 2007 828 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 3452 10 Size: 54 1425 343 240 1380 2775 Fragments arranged by size: 2775 1425 1380 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2769 2991 3038 4175 4534 4693 518 Size: 648 1603 222 47 1137 359 159 2042 Fragments arranged by size: 2042 1603 1137 648 359 222 159 47 MspA1I CmG'CkG Cuts at: 1606 2059 2629 3633 4310 5458 5703 1606 Size: 453 570 1004 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 570 453 245 NcoI C'CATG_G Cuts at: 513 1065 3084 513 Size: 552 2019 3646 Fragments arranged by size: 3646 2019 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2536 3518 3575 5120 1132 Size: 97 1307 982 57 1545 2229 Fragments arranged by size: 2229 1545 1307 982 97 57 PasI CC'CwG_GG Cuts at: 1348 2266 1348 Size: 918 5299 PciI A'CATG_T Cuts at: 2532 5116 2532 Size: 2584 3633 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PfoI T'CCnGG_A Cuts at: 3045 3241 3399 3045 Size: 196 158 5863 Fragments arranged by size: 5863 196 158 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2271 2433 2271 Size: 162 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2980 3285 2980 Size: 305 5912 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2273 2305 2273 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 2046 2187 2246 2976 3007 3063 3569 830 Size: 1216 141 59 730 31 56 506 3478 Fragments arranged by size: 3478 1216 730 506 141 59 56 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2059 2629 2059 Size: 570 5647 SacI G_AGCT'C Cuts at: 2223 3187 2223 Size: 964 5253 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 2433 2433 Size: 6217 SapI GCTCTTCn'nnn_ Cuts at: 3374 3374 Size: 6217 SbfI CC_TGCA'GG Cuts at: 2976 3063 3569 2976 Size: 87 506 5624 Fragments arranged by size: 5624 506 87 ScaI AGT'ACT Cuts at: 2291 3053 4465 6177 2291 Size: 762 1412 1712 2331 Fragments arranged by size: 2331 1712 1412 762 SexAI A'CCwGG_T Cuts at: 3144 3144 Size: 6217 SfcI C'TryA_G Cuts at: 826 1045 2042 2183 2242 2972 3003 3059 3565 Size: 219 997 141 59 730 31 56 506 Cuts at: 3565 4700 5381 5572 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 997 730 681 506 219 191 141 59 56 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3422 4321 5222 5484 820 Size: 197 926 1479 899 901 262 1553 Fragments arranged by size: 1553 1479 926 901 899 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 3029 1517 Size: 1512 4705 StyI C'CwwG_G Cuts at: 513 1065 2373 2787 3084 3168 3656 513 Size: 552 1308 414 297 84 488 3074 Fragments arranged by size: 3074 1308 552 488 414 297 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 2958 3264 4244 4261 4414 4599 Size: 1255 957 421 306 980 17 153 185 Cuts at: 4599 5044 325 Size: 445 1498 Fragments arranged by size: 1498 1255 980 957 445 421 306 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2010 2289 3051 Size: 274 80 33 51 1443 33 279 762 Cuts at: 3051 4463 6175 96 Size: 1412 1712 138 Fragments arranged by size: 1712 1443 1412 762 279 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 2319 2351 6177 6209 2189 Size: 32 98 32 3826 32 2197 Fragments arranged by size: 3826 2197 98 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2822 1316 Size: 1506 4711 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2108 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2542 4025 276 Size: 53 83 186 936 1008 1483 2468 Fragments arranged by size: 2468 1483 1008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SfiI SfoI SrfI SwaI