Restriction map for
pFN21AB5913
(Linear) (Six-base) MAPSORT of: KIBB5913.seq Check: 3349 from: 1 to: 1512
KIBB5913.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 749 1512
Size: 749 763
AatII G_ACGT'C
Cuts at: 0 539 1512
Size: 539 973
AccI GT'mk_AC
Cuts at: 0 449 1512
Size: 449 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1512
Size: 1084 428
AflII C'TTAA_G
Cuts at: 0 1417 1512
Size: 1417 95
AflIII A'CryG_T
Cuts at: 0 527 1512
Size: 527 985
AleI CACnn'nnGTG
Cuts at: 0 764 1512
Size: 764 748
AlwNI CAG_nnn'CTG
Cuts at: 0 125 1006 1062 1443 1512
Size: 125 881 56 381 69
Fragments arranged by size:
881 381 125 69 56
ApaI G_GGCC'C
Cuts at: 0 979 1284 1512
Size: 979 305 228
Fragments arranged by size:
979 305 228
AvaI C'yCGr_G
Cuts at: 0 1073 1181 1512
Size: 1073 108 331
Fragments arranged by size:
1073 331 108
BamHI G'GATC_C
Cuts at: 0 387 1512
Size: 387 1125
BanI G'GyrC_C
Cuts at: 0 670 1512
Size: 670 842
BanII G_rGCy'C
Cuts at: 0 78 218 866 979 1182 1284 1512
Size: 78 140 648 113 203 102 228
Fragments arranged by size:
648 228 203 140 113 102 78
BciVI GTATCCnnnnn_n'
Cuts at: 0 1218 1512
Size: 1218 294
BglI GCCn_nnn'nGGC
Cuts at: 0 73 442 1512
Size: 73 369 1070
Fragments arranged by size:
1070 369 73
BlpI GC'TnA_GC
Cuts at: 0 77 1187 1512
Size: 77 1110 325
Fragments arranged by size:
1110 325 77
Bme1580I G_kGCm'C
Cuts at: 0 979 1284 1512
Size: 979 305 228
Fragments arranged by size:
979 305 228
BmgBI CAC'GTC
Cuts at: 0 557 1512
Size: 557 955
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 1512
Size: 116 1396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 274 1259 1378 1512
Size: 232 42 985 119 134
Fragments arranged by size:
985 232 134 119 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1235 1512
Size: 1235 277
BsaHI Gr'CG_yC
Cuts at: 0 536 1512
Size: 536 976
BsaWI w'CCGG_w
Cuts at: 0 476 875 1322 1512
Size: 476 399 447 190
Fragments arranged by size:
476 447 399 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 314 344 395 425 1512
Size: 130 30 154 30 51 30 1087
Fragments arranged by size:
1087 154 130 51 30 30 30
BseYI C'CCAG_C
Cuts at: 0 432 1284 1512
Size: 432 852 228
Fragments arranged by size:
852 432 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1011 1512
Size: 377 634 501
Fragments arranged by size:
634 501 377
BsiEI CG_ry'CG
Cuts at: 0 477 1512
Size: 477 1035
BsiHKAI G_wGCw'C
Cuts at: 0 218 1182 1512
Size: 218 964 330
Fragments arranged by size:
964 330 218
BsmI GAATG_Cn'
Cuts at: 0 1074 1512
Size: 1074 438
Bsp1286I G_dGCh'C
Cuts at: 0 78 218 866 979 1182 1284 1512
Size: 78 140 648 113 203 102 228
Fragments arranged by size:
648 228 203 140 113 102 78
BspEI T'CCGG_A
Cuts at: 0 1322 1512
Size: 1322 190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 749 1047 1163 1512
Size: 503 246 298 116 349
Fragments arranged by size:
503 349 298 246 116
BsrBI CCG'CTC
Cuts at: 0 1191 1512
Size: 1191 321
BsrDI GCAATG_nn'
Cuts at: 0 218 992 1512
Size: 218 774 520
Fragments arranged by size:
774 520 218
BstAPI GCAn_nnn'nTGC
Cuts at: 0 634 1512
Size: 634 878
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 1170 1512
Size: 766 404 342
Fragments arranged by size:
766 404 342
BstYI r'GATC_y
Cuts at: 0 387 917 1512
Size: 387 530 595
Fragments arranged by size:
595 530 387
Bsu36I CC'TnA_GG
Cuts at: 0 1453 1512
Size: 1453 59
BtgI C'CryG_G
Cuts at: 0 519 652 1079 1512
Size: 519 133 427 433
Fragments arranged by size:
519 433 427 133
BtsI GCAGTG_nn'
Cuts at: 0 205 1214 1512
Size: 205 1009 298
Fragments arranged by size:
1009 298 205
DraIII CAC_nnn'GTG
Cuts at: 0 1143 1512
Size: 1143 369
EaeI y'GGCC_r
Cuts at: 0 65 443 649 909 1445 1512
Size: 65 378 206 260 536 67
Fragments arranged by size:
536 378 260 206 67 65
EarI CTCTTCn'nnn_
Cuts at: 0 848 1369 1512
Size: 848 521 143
Fragments arranged by size:
848 521 143
EcoICRI GAG'CTC
Cuts at: 0 216 1180 1512
Size: 216 964 332
Fragments arranged by size:
964 332 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 274 1084 1259 1378 1512
Size: 232 42 810 175 119 134
Fragments arranged by size:
810 232 175 134 119 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1417 1512
Size: 1018 399 95
Fragments arranged by size:
1018 399 95
EcoO109I rG'GnC_Cy
Cuts at: 0 258 266 428 975 976 1167 1280 1281
Size: 258 8 162 547 1 191 113 1
Cuts at: 1281 1481 1512
Size: 200 31
Fragments arranged by size:
547 258 200 191 162 113 31 8
1 1
EcoRV GAT'ATC
Cuts at: 0 548 1512
Size: 548 964
HaeII r_GCGC'y
Cuts at: 0 363 680 1512
Size: 363 317 832
Fragments arranged by size:
832 363 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 395 427 1192 1224 1390 1422
Size: 130 32 233 32 765 32 166 32
Cuts at: 1422 1512
Size: 90
Fragments arranged by size:
765 233 166 130 90 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1356 1512
Size: 1356 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1512
Size: 128 1384
MscI TGG'CCA
Cuts at: 0 67 1447 1512
Size: 67 1380 65
Fragments arranged by size:
1380 67 65
MslI CAynn'nnrTG
Cuts at: 0 764 986 1033 1512
Size: 764 222 47 479
Fragments arranged by size:
764 479 222 47
MspA1I CmG'CkG
Cuts at: 0 54 624 1512
Size: 54 570 888
Fragments arranged by size:
888 570 54
NcoI C'CATG_G
Cuts at: 0 1079 1512
Size: 1079 433
NspI r_CATG'y
Cuts at: 0 531 1512
Size: 531 981
PasI CC'CwG_GG
Cuts at: 0 261 1512
Size: 261 1251
PciI A'CATG_T
Cuts at: 0 527 1512
Size: 527 985
PfoI T'CCnGG_A
Cuts at: 0 1040 1236 1394 1512
Size: 1040 196 158 118
Fragments arranged by size:
1040 196 158 118
PpuMI rG'GwC_Cy
Cuts at: 0 266 428 1512
Size: 266 162 1084
Fragments arranged by size:
1084 266 162
PspOMI G'GGCC_C
Cuts at: 0 975 1280 1512
Size: 975 305 232
Fragments arranged by size:
975 305 232
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 268 300 1512
Size: 268 32 1212
Fragments arranged by size:
1212 268 32
PstI C_TGCA'G
Cuts at: 0 41 182 241 971 1002 1058 1512
Size: 41 141 59 730 31 56 454
Fragments arranged by size:
730 454 141 59 56 41 31
PvuII CAG'CTG
Cuts at: 0 54 624 1512
Size: 54 570 888
Fragments arranged by size:
888 570 54
SacI G_AGCT'C
Cuts at: 0 218 1182 1512
Size: 218 964 330
Fragments arranged by size:
964 330 218
SanDI GG'GwC_CC
Cuts at: 0 428 1512
Size: 428 1084
SapI GCTCTTCn'nnn_
Cuts at: 0 1369 1512
Size: 1369 143
SbfI CC_TGCA'GG
Cuts at: 0 971 1058 1512
Size: 971 87 454
Fragments arranged by size:
971 454 87
ScaI AGT'ACT
Cuts at: 0 286 1048 1512
Size: 286 762 464
Fragments arranged by size:
762 464 286
SexAI A'CCwGG_T
Cuts at: 0 1139 1512
Size: 1139 373
SfcI C'TryA_G
Cuts at: 0 37 178 237 967 998 1054 1512
Size: 37 141 59 730 31 56 458
Fragments arranged by size:
730 458 141 59 56 37 31
SmlI C'TyrA_G
Cuts at: 0 1417 1512
Size: 1417 95
StuI AGG'CCT
Cuts at: 0 1024 1512
Size: 1024 488
StyI C'CwwG_G
Cuts at: 0 368 782 1079 1163 1512
Size: 368 414 297 84 349
Fragments arranged by size:
414 368 349 297 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 953 1259 1512
Size: 532 421 306 253
Fragments arranged by size:
532 421 306 253
TatI w'GTAC_w
Cuts at: 0 5 284 1046 1512
Size: 5 279 762 466
Fragments arranged by size:
762 466 279 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 314 346 1512
Size: 184 32 98 32 1166
Fragments arranged by size:
1166 184 98 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 817 1512
Size: 817 695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 1512
Size: 103 1409
ZraI GAC'GTC
Cuts at: 0 537 1512
Size: 537 975
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AlwNI
ApaI AvaI BamHI BanI BanII BciVI BglI BlpI
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PfoI PpuMI PspOMI PsrI PstI
PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BclI BfrBI BglII BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BseRI BsiWI BsmBI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoRI FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacII SalI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI XmnI