(Circular) (Six-base) MAPSORT of: pFN21AB5911.seq Check: 1869 from: 1 to: 6217 pFN21AB5911 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2910 3177 3181 2910 Size: 267 4 5946 Fragments arranged by size: 5946 267 4 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2064 3560 1937 Size: 127 1496 4594 Fragments arranged by size: 4594 1496 127 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 4280 5664 5932 1632 Size: 689 1959 1384 268 1917 Fragments arranged by size: 1959 1917 1384 689 268 AfeI AGC'GCT Cuts at: 3037 3037 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 2537 820 Size: 197 1520 4500 Fragments arranged by size: 4500 1520 197 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 3356 1166 Size: 2190 4027 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2312 3185 1184 Size: 1128 873 4216 Fragments arranged by size: 4216 1128 873 AlwNI CAG_nnn'CTG Cuts at: 3123 3133 3217 3334 3960 5532 3123 Size: 10 84 117 626 1572 3808 Fragments arranged by size: 3808 1572 626 117 84 10 ApaI G_GGCC'C Cuts at: 2252 3508 2252 Size: 1256 4961 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2566 3083 3482 3542 1870 Size: 73 623 517 399 60 4545 Fragments arranged by size: 4545 623 517 399 73 60 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2201 2340 3010 3165 Size: 325 217 627 351 63 139 670 155 Cuts at: 3165 3538 4993 618 Size: 373 1455 1842 Fragments arranged by size: 1842 1455 670 627 373 351 325 217 155 139 63 BanII G_rGCy'C Cuts at: 1391 2252 2418 2466 2697 2949 3508 3536 1391 Size: 861 166 48 231 252 559 28 4072 Fragments arranged by size: 4072 861 559 252 231 166 48 28 BbeI G_GCGC'C Cuts at: 2142 2344 2142 Size: 202 6015 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 928 Size: 971 128 5118 Fragments arranged by size: 5118 971 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2697 2731 4408 4442 1903 Size: 34 760 34 1677 34 3678 Fragments arranged by size: 3678 1677 760 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2720 4109 5319 2720 Size: 1389 1210 3618 Fragments arranged by size: 3618 1389 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2814 2911 3178 Size: 107 122 71 631 605 1142 97 267 Cuts at: 3178 3404 4828 136 Size: 226 1424 1525 Fragments arranged by size: 1525 1424 1142 631 605 267 226 122 107 97 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3467 3634 3467 Size: 167 6050 Bme1580I G_kGCm'C Cuts at: 1790 2252 3015 3508 4278 5434 1790 Size: 462 763 493 770 1156 2573 Fragments arranged by size: 2573 1156 770 763 493 462 BmgBI CAC'GTC Cuts at: 1318 2025 1318 Size: 707 5510 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2915 2951 3050 3083 3160 4877 1239 Size: 1226 450 36 99 33 77 1717 2579 Fragments arranged by size: 2579 1717 1226 450 99 77 36 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2497 4008 493 Size: 711 1293 1511 2702 Fragments arranged by size: 2702 1511 1293 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2139 2341 4024 Size: 53 83 186 936 528 78 202 1683 Cuts at: 4024 4406 5041 275 Size: 382 635 1451 Fragments arranged by size: 1683 1451 936 635 528 382 202 186 83 78 53 BsaWI w'CCGG_w Cuts at: 3093 4650 5048 5322 5469 5880 3093 Size: 1557 398 274 147 411 3430 Fragments arranged by size: 3430 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 2654 2684 3158 Size: 30 165 30 984 30 237 30 474 Cuts at: 3158 3188 3463 3493 1178 Size: 30 275 30 3902 Fragments arranged by size: 3902 984 474 275 237 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3329 1186 Size: 2143 4074 BseYI C'CCAG_C Cuts at: 3157 3289 3424 5420 5916 3157 Size: 132 135 1996 496 3458 Fragments arranged by size: 3458 1996 496 135 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 3334 1163 Size: 573 504 1094 4046 Fragments arranged by size: 4046 1094 573 504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2148 3667 4428 5456 1540 Size: 275 186 147 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 147 BsiHKAI G_wGCw'C Cuts at: 2100 2418 2901 3473 4278 4363 5434 2100 Size: 318 483 572 805 85 1071 2883 Fragments arranged by size: 2883 1071 805 572 483 318 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2252 2418 2466 2697 2901 2949 Size: 399 310 152 166 48 231 204 48 Cuts at: 2949 3015 3473 3508 3536 4278 4363 5434 1391 Size: 66 458 35 28 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 310 231 204 166 152 85 66 48 48 35 28 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 2658 2910 2964 3177 3181 3572 844 Size: 1685 129 252 54 213 4 391 3489 Fragments arranged by size: 3489 1685 391 252 213 129 54 4 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2772 4861 5048 1700 Size: 110 962 2089 187 2869 Fragments arranged by size: 2869 2089 962 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 3142 3388 1925 Size: 1217 246 4754 Fragments arranged by size: 4754 1217 246 BssSI C'ACGA_G Cuts at: 2612 4277 5289 2612 Size: 1665 1012 3540 Fragments arranged by size: 3540 1665 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3123 794 Size: 2329 3888 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2173 2623 1184 Size: 989 450 4778 Fragments arranged by size: 4778 989 450 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2170 Size: 6217 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 2812 2949 2997 3417 513 Size: 552 1098 649 137 48 420 3313 Fragments arranged by size: 3313 1098 649 552 420 137 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2844 3257 3733 4527 4547 2844 Size: 413 476 794 20 4514 Fragments arranged by size: 4514 794 476 413 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2365 5224 809 Size: 507 1049 2859 1802 Fragments arranged by size: 2859 1802 1049 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2145 2160 Size: 54 1425 50 275 18 285 30 15 Cuts at: 2160 2994 3000 3303 3447 3664 4553 6182 8 Size: 834 6 303 144 217 889 1629 43 Fragments arranged by size: 1629 1425 889 834 303 285 275 217 144 54 50 43 30 18 15 6 EagI C'GGCC_G Cuts at: 1537 1812 2145 3664 1537 Size: 275 333 1519 4090 Fragments arranged by size: 4090 1519 333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2687 3350 4147 1353 Size: 1334 663 797 3423 Fragments arranged by size: 3423 1334 797 663 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2416 2416 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2465 2915 2951 3050 3083 3160 Size: 393 689 144 450 36 99 33 77 Cuts at: 3160 4280 4877 5664 5932 1239 Size: 1120 597 787 268 1524 Fragments arranged by size: 1524 1120 787 689 597 450 393 268 144 99 77 36 33 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2300 2672 2930 2192 Size: 108 372 258 5479 Fragments arranged by size: 5479 372 258 108 EcoO109I rG'GnC_Cy Cuts at: 2249 2305 2677 2923 3504 3505 2249 Size: 56 372 246 581 1 4961 Fragments arranged by size: 4961 581 372 246 56 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 6217 FspI TGC'GCA Cuts at: 2654 3275 3383 4723 2654 Size: 621 108 1340 4148 Fragments arranged by size: 4148 1340 621 108 HaeII r_GCGC'y Cuts at: 1678 2142 2156 2171 2344 2850 3039 3328 5364 Size: 464 14 15 173 506 189 289 2036 Cuts at: 5364 1678 Size: 2531 Fragments arranged by size: 2531 2036 506 464 289 189 173 15 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3413 3445 4858 4890 4932 Size: 32 135 32 1841 32 1413 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2626 1841 1413 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2138 2340 2138 Size: 202 6015 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2997 5331 5515 1305 Size: 1692 2334 184 2007 Fragments arranged by size: 2334 2007 1692 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2996 3305 10 Size: 54 1425 343 285 879 309 2922 Fragments arranged by size: 2922 1425 879 343 309 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2334 2450 2552 3002 3008 3356 Size: 648 1162 6 116 102 450 6 348 Cuts at: 3356 4175 4534 4693 518 Size: 819 359 159 2042 Fragments arranged by size: 2042 1162 819 648 450 359 348 159 116 102 6 6 MspA1I CmG'CkG Cuts at: 1606 2439 2755 2872 3028 3232 3331 3419 3445 Size: 833 316 117 156 204 99 88 26 Cuts at: 3445 3633 4310 5458 5703 1606 Size: 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 833 677 316 245 204 188 156 117 99 88 26 NarI GG'CG_CC Cuts at: 2139 2341 2139 Size: 202 6015 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5665 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2333 2980 3388 3575 5120 1132 Size: 97 1104 647 408 187 1545 2229 Fragments arranged by size: 2229 1545 1104 647 408 187 97 PasI CC'CwG_GG Cuts at: 1348 3508 1348 Size: 2160 4057 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2839 3133 1108 Size: 1731 294 4192 Fragments arranged by size: 4192 1731 294 PfoI T'CCnGG_A Cuts at: 2892 2892 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2305 Size: 6217 PshAI GACnn'nnGTC Cuts at: 1613 2583 1613 Size: 970 5247 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2248 3504 2248 Size: 1256 4961 PstI C_TGCA'G Cuts at: 830 2438 2754 3569 830 Size: 1608 316 815 3478 Fragments arranged by size: 3478 1608 815 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2872 3331 3445 2872 Size: 459 114 5644 Fragments arranged by size: 5644 459 114 SacI G_AGCT'C Cuts at: 2418 2418 Size: 6217 SacII CC_GC'GG Cuts at: 3420 3420 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SexAI A'CCwGG_T Cuts at: 2271 3132 2271 Size: 861 5356 SfcI C'TryA_G Cuts at: 826 1045 2434 2750 3247 3565 4700 5381 5572 Size: 219 1389 316 497 318 1135 681 191 Cuts at: 5572 826 Size: 1471 Fragments arranged by size: 1471 1389 1135 681 497 318 316 219 191 SfoI GGC'GCC Cuts at: 2140 2342 2140 Size: 202 6015 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2537 4321 5222 5484 820 Size: 197 926 594 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 594 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 2333 3388 3575 1132 Size: 1201 1055 187 3774 Fragments arranged by size: 3774 1201 1055 187 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2936 1517 Size: 1419 4798 StyI C'CwwG_G Cuts at: 513 1065 2406 2673 3656 513 Size: 552 1341 267 983 3074 Fragments arranged by size: 3074 1341 983 552 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 4244 4261 4414 4599 5044 325 Size: 1255 600 2064 17 153 185 445 1498 Fragments arranged by size: 2064 1498 1255 600 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2454 4463 6175 Size: 274 80 33 51 1443 477 2009 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 2009 1712 1443 477 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 3080 3112 3158 3190 3463 3495 6177 Size: 32 661 32 46 32 273 32 2682 Cuts at: 6177 6209 2387 Size: 32 2395 Fragments arranged by size: 2682 2395 661 273 46 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2785 3004 3058 3154 2341 Size: 444 219 54 96 5404 Fragments arranged by size: 5404 444 219 96 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2240 4346 2240 Size: 2106 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI