Restriction map for
pFN21AB5906
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BspDI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I MamI
MflI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1078 TCCGGA T^CCGGA
AocI 454 CCTCAGG CC^T[ACGT]AGG
AseI 964 ATTAAT AT^TAAT
AsnI 964 ATTAAT AT^TAAT
BanII 255 GAGCTC G[GA]GC[CT]^C
BfaI 401 CTAG C^TAG
BsaWI 1078 TCCGGA [AT]^CCGG[AT]
BseAI 1078 TCCGGA T^CCGGA
BsmI 1327 GAATGC GAATGC(1/-1)
Bsp1407I 971 TGTACA T^GTACA
BspEI 1078 TCCGGA T^CCGGA
BspHI 822 TCATGA T^CATGA
BsrDI 1139 GCAATG GCAATG(2/0)
BsrGI 971 TGTACA T^GTACA
BstF5I 1407 GGATG GGATG(2/0)
Bsu36I 454 CCTCAGG CC^T[ACGT]AGG
Eco81I 454 CCTCAGG CC^T[ACGT]AGG
EcoRI 1072 GAATTC G^AATTC
FauI 1463 GCGGG CCCGC(4/6)
FokI 1407 GGATG GGATG(9/13)
HincII 207 GTCAAC GT[CT]^[GA]AC
HindII 207 GTCAAC GT[CT]^[GA]AC
MaeI 401 CTAG C^TAG
MaeII 819 ACGT A^CGT
MboII 166 TCTTC GAAGA(8/7)
MfeI 890 CAATTG C^AATTG
MroI 1078 TCCGGA T^CCGGA
MslI 858 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 890 CAATTG C^AATTG
NcoI 1172 CCATGG C^CATGG
PpuMI 1167 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 964 ATTAAT AT^TAAT
RcaI 822 TCATGA T^CATGA
SacI 255 GAGCTC GAGCT^C
SexAI 1374 ACCAGGT A^CC[AT]GGT
SfcI 387 CTACAG C^T[GA][CT]AG
SmaI 61 CCCGGG CCC^GGG
SspBI 971 TGTACA T^GTACA
StuI 870 AGGCCT AGG^CCT
TfiI 1534 GATTC G^A[AT]TC
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 646 CGCG CG^CG
1459 CGCG
AcsI 790 AAATTT [GA]^AATT[CT]
1072 GAATTC
AfaI 480 GTAC GT^AC
972 GTAC
AlwI 814 GGATC GGATC(4/5)
1557 GGATC
ApoI 790 AAATTT [GA]^AATT[CT]
1072 GAATTC
AspHI 255 GAGCTC G[AT]GC[AT]^C
1546 GAGCAC
BglI 740 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1178 GCCGTGGAGGC
BmyI 255 GAGCTC G[AGT]GC[ACT]^C
1546 GAGCAC
BsaI 1014 GGTCTC GGTCTC(1/5)
1095 GGTCTC
BsiHKAI 255 GAGCTC G[AT]GC[AT]^C
1546 GAGCAC
BsmAI 1015 GTCTC GTCTC(1/5)
1096 GTCTC
Bsp1286I 255 GAGCTC G[AGT]GC[ACT]^C
1546 GAGCAC
BstUI 646 CGCG CG^CG
1459 CGCG
EcoT14I 810 CCAAGG C^C[AT][AT]GG
1172 CCATGG
KspI 645 CCGCGG CCGC^GG
1458 CCGCGG
MvnI 646 CGCG CG^CG
1459 CGCG
PleI 826 GACTC GAGTC(4/5)
895 GAGTC
RsaI 480 GTAC GT^AC
972 GTAC
SacII 645 CCGCGG CCGC^GG
1458 CCGCGG
StyI 810 CCAAGG C^C[AT][AT]GG
1172 CCATGG
TaqI 229 TCGA T^CGA
1440 TCGA
Tsp45I 770 GTGAC ^GT[GC]AC
1271 GTGAC
TthHB8I 229 TCGA T^CGA
1440 TCGA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaI AvaII BalI
BbvI BcnI BsaJI BseRI BsiYI
BslI BsmFI BsoBI BsrI BssKI
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I TspRI