Restriction map for
pFN21AB5906
(Circular) (Six-base) MAPSORT of: pFN21AB5906.seq Check: 1074 from: 1 to: 6283
pFN21AB5906 6283 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 3023 3024 4346 5730 5998 1632
Size: 498 893 1 1322 1384 268 1917
Fragments arranged by size:
1917 1384 1322 893 498 268 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 5012 5012
Size: 6283
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 4026 5598 4026
Size: 1572 4711
ApaLI G'TGCA_C
Cuts at: 4340 5496 4340
Size: 1156 5127
ApoI r'AATT_y
Cuts at: 1493 2795 3077 3592 3818 3970 6079 1493
Size: 1302 282 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 1302 515 282 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2970 4837 160
Size: 872 1938 1867 1606
Fragments arranged by size:
1938 1867 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2332 2808 3608 1870
Size: 73 123 266 476 800 4545
Fragments arranged by size:
4545 800 476 266 123 73
BamHI G'GATC_C
Cuts at: 3613 3980 3613
Size: 367 5916
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3604 5059 618
Size: 325 217 627 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2264 3602 1391
Size: 873 1338 4072
Fragments arranged by size:
4072 1338 873
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4175 5385 4175
Size: 1210 5073
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2751 3189 4894
Size: 107 122 71 631 605 1079 438 1705
Cuts at: 4894 136
Size: 1525
Fragments arranged by size:
1705 1525 1079 631 605 438 122 107
71
BglII A'GATC_T
Cuts at: 6278 6278
Size: 6283
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 4344 5500 1790
Size: 2554 1156 2573
Fragments arranged by size:
2573 2554 1156
BmgBI CAC'GTC
Cuts at: 1318 2825 1318
Size: 1507 4776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4014 4972
Size: 416 53 83 399 24 1814 783 958
Cuts at: 4972 442
Size: 1753
Fragments arranged by size:
1814 1753 958 783 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4943 1239
Size: 3704 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4408 5273 5571 4408
Size: 865 298 5120
Fragments arranged by size:
5120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3025 3106 4946 882
Size: 617 1526 81 1840 2219
Fragments arranged by size:
2219 1840 1526 617 81
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3083 4716 5114 5388 5535 5946 3083
Size: 1633 398 274 147 411 3420
Fragments arranged by size:
3420 1633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3563 3593 1178
Size: 30 165 30 2160 30 3868
Fragments arranged by size:
3868 2160 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3183 3240 3447 1186
Size: 1997 57 207 4022
Fragments arranged by size:
4022 1997 207 57
BseYI C'CCAG_C
Cuts at: 2061 2082 2321 5486 5982 2061
Size: 21 239 3165 496 2362
Fragments arranged by size:
3165 2362 496 239 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5710
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2264 3555 4344 4429 5500 2264
Size: 1291 789 85 1071 3047
Fragments arranged by size:
3047 1291 1071 789 85
BsmI GAATG_Cn'
Cuts at: 3338 3794 3887 3338
Size: 456 93 5734
Fragments arranged by size:
5734 456 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 3555 3602 4344 4429 5500 1391
Size: 399 474 1291 47 742 85 1071 2174
Fragments arranged by size:
2174 1291 1071 742 474 399 85 47
BspEI T'CCGG_A
Cuts at: 3083 5946 3083
Size: 2863 3420
BspHI T'CATG_A
Cuts at: 2827 4172 5902 2827
Size: 1345 1730 3208
Fragments arranged by size:
3208 1730 1345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4170 1394
Size: 429 2347 3507
Fragments arranged by size:
3507 2347 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3151 4778 4952 65
Size: 1183 1903 1627 174 1396
Fragments arranged by size:
1903 1627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2976 96
Size: 2880 3403
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6283
BstXI CCAn_nnnn'nTGG
Cuts at: 2045 2228 2606 2045
Size: 183 378 5722
Fragments arranged by size:
5722 378 183
BstYI r'GATC_y
Cuts at: 1972 3613 3980 4365 4382 5834 5920 5932 6278
Size: 1641 367 385 17 1452 86 12 346
Cuts at: 6278 1972
Size: 1977
Fragments arranged by size:
1977 1641 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2460 2460
Size: 6283
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2641 2650 2746 2779 3177
Size: 552 939 132 505 9 96 33 398
Cuts at: 3177 3184 3463 3475 513
Size: 7 279 12 3321
Fragments arranged by size:
3321 939 552 505 398 279 132 96
33 12 9 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 2578 2671 2716 3214 3799 4593 4613 2503
Size: 75 93 45 498 585 794 20 4173
Fragments arranged by size:
4173 794 585 498 93 75 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3133 3168 529
Size: 35 2569 35 3644
Fragments arranged by size:
3644 2569 35 35
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2179 2305
Size: 54 1425 50 275 18 325 24 126
Cuts at: 2305 2536 2743 3220 3730 4619 6248 8
Size: 231 207 477 510 889 1629 43
Fragments arranged by size:
1629 1425 889 510 477 325 275 231
207 126 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4213 1353
Size: 2860 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2784 4870 5244 5390 387
Size: 1064 138 1195 2086 374 146 1280
Fragments arranged by size:
2086 1280 1195 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2262 2262
Size: 6283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 3023 3024 4346 4943 5730 5998
Size: 393 498 893 1 1322 597 787 268
Cuts at: 5998 1239
Size: 1524
Fragments arranged by size:
1524 1322 893 787 597 498 393 268
1
EcoO109I rG'GnC_Cy
Cuts at: 2049 3173 3349 2049
Size: 1124 176 4983
Fragments arranged by size:
4983 1124 176
EcoRI G'AATT_C
Cuts at: 3077 3592 3970 3077
Size: 515 378 5390
Fragments arranged by size:
5390 515 378
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 2804 2836 4924
Size: 32 135 32 962 32 238 32 2088
Cuts at: 4924 4956 4998 5030 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2088 962 238 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2214 3627 3873 669
Size: 988 281 276 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 2895 3882 2895
Size: 987 5296
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5397 5581 1305
Size: 4092 184 2007
Fragments arranged by size:
4092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 2181 2307 2538 10
Size: 54 1425 343 325 24 126 231 3755
Fragments arranged by size:
3755 1425 343 325 231 126 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 4241 4600 4759 518
Size: 648 1701 1374 359 159 2042
Fragments arranged by size:
2042 1701 1374 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2619 2652 3465 3699 4376 5524 5769 1606
Size: 1013 33 813 234 677 1148 245 2120
Fragments arranged by size:
2120 1148 1013 813 677 245 234 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 3177 513
Size: 552 939 1173 3619
Fragments arranged by size:
3619 1173 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 3641 5186 1132
Size: 97 2412 1545 2229
Fragments arranged by size:
2412 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2221 1348
Size: 873 5410
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6283
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3173 3173
Size: 6283
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PstI C_TGCA'G
Cuts at: 830 3635 830
Size: 2805 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
SacI G_AGCT'C
Cuts at: 2264 2264
Size: 6283
SacII CC_GC'GG
Cuts at: 2653 3466 2653
Size: 813 5470
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SexAI A'CCwGG_T
Cuts at: 3379 3379
Size: 6283
SfcI C'TryA_G
Cuts at: 826 1045 2392 3631 4766 5447 5638 826
Size: 219 1347 1239 1135 681 191 1471
Fragments arranged by size:
1471 1347 1239 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 2068 3610 1872
Size: 196 1542 4545
Fragments arranged by size:
4545 1542 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4387 5288 5550 820
Size: 197 926 2444 901 262 1553
Fragments arranged by size:
2444 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SrfI GCCC'GGGC
Cuts at: 2068 2068
Size: 6283
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 2877 1517
Size: 1360 4923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2815 3177 3722 513
Size: 552 939 811 362 545 3074
Fragments arranged by size:
3074 939 811 552 545 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2870 4310 4327 4480 4665 5110
Size: 1255 495 795 1440 17 153 185 445
Cuts at: 5110 325
Size: 1498
Fragments arranged by size:
1498 1440 1255 795 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 2976 4529
Size: 274 80 33 51 1443 507 492 1553
Cuts at: 4529 6241 96
Size: 1712 138
Fragments arranged by size:
1712 1553 1443 507 492 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 2066 3608 1870
Size: 196 1542 4545
Fragments arranged by size:
4545 1542 196
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SanDI SapI SfiI SfoI SwaI
XcmI