Restriction map for
pFN21AB5902
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BalI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
MamI MfeI MluI MluNI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 608 TCCGGA T^CCGGA
Alw44I 451 GTGCAC G^TGCAC
AocI 456 CCTCAGG CC^T[ACGT]AGG
ApaLI 451 GTGCAC G^TGCAC
AviII 326 TGCGCA TGC^GCA
BamHI 1084 GGATCC G^GATCC
BbeI 261 GGCGCC GGCGC^C
BbsI 1424 GAAGAC GAAGAC(2/6)
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
BlpI 303 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 303 GCTGAGC GC^T[ACGT]AGC
BpuAI 1424 GAAGAC GAAGAC(2/6)
BseAI 608 TCCGGA T^CCGGA
BsiEI 375 CGGCCG CG[GA][CT]^CG
BsmBI 750 CGTCTC CGTCTC(1/5)
BspEI 608 TCCGGA T^CCGGA
BsrDI 1230 GCAATG GCAATG(2/0)
BssHII 420 GCGCGC G^CGCGC
BstEII 1076 GGTCACC G^GT[ACGT]ACC
BstPI 1076 GGTCACC G^GT[ACGT]ACC
BstXI 1305 CCAACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 456 CCTCAGG CC^T[ACGT]AGG
CelII 303 GCTGAGC GC^T[ACGT]AGC
EagI 375 CGGCCG C^GGCCG
EclXI 375 CGGCCG C^GGCCG
Eco52I 375 CGGCCG C^GGCCG
Eco81I 456 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1076 GGTCACC G^GT[ACGT]ACC
FspI 326 TGCGCA TGC^GCA
HaeII 261 GGCGCC [GA]GCGC^[CT]
HindIII 669 AAGCTT A^AGCTT
KasI 261 GGCGCC G^GCGCC
KspI 446 CCGCGG CCGC^GG
MaeII 749 ACGT A^CGT
MroI 608 TCCGGA T^CCGGA
MslI 1050 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 261 GGCGCC GG^CGCC
NcoI 698 CCATGG C^CATGG
SacI 1146 GAGCTC GAGCT^C
SacII 446 CCGCGG CCGC^GG
SapI 170 GAAGAGC GCTCTTC(1/4)
SfoI 261 GGCGCC GGC^GCC
SgrAI 429 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 1467 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 261 GGCGCC G[GA]^CG[CT]C
427 GACGCC
AspHI 451 GTGCAC G[AT]GC[AT]^C
1146 GAGCTC
AspI 547 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
867 GACCCGGTC
BfaI 958 CTAG C^TAG
1292 CTAG
BsaHI 261 GGCGCC G[GA]^CG[CT]C
427 GACGCC
BsiHKAI 451 GTGCAC G[AT]GC[AT]^C
1146 GAGCTC
BsmAI 932 GAGAC GTCTC(1/5)
751 GTCTC
BspMI 994 ACCTGC ACCTGC(4/8)
820 GCAGGT
BsrFI 377 GCCGGC [GA]^CCGG[CT]
430 GCCGGC
Cfr10I 377 GCCGGC [GA]^CCGG[CT]
430 GCCGGC
DsaI 446 CCGCGG C^C[GA][CT]GG
698 CCATGG
EaeI 228 CGGCCA [CT]^GGCC[GA]
375 CGGCCG
EarI 676 CTCTTC CTCTTC(1/4)
170 GAAGAG
EcoNI 495 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
901 CCTGGGGGAGG
HgaI 427 GACGC GACGC(5/10)
356 GCGTC
Hin1I 261 GGCGCC G[GA]^CG[CT]C
427 GACGCC
Ksp632I 676 CTCTTC CTCTTC(1/4)
170 GAAGAG
MaeI 958 CTAG C^TAG
1292 CTAG
NaeI 377 GCCGGC GCC^GGC
430 GCCGGC
NgoMIV 377 GCCGGC G^CCGGC
430 GCCGGC
NlaIII 699 CATG CATG^
759 CATG
PflFI 547 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
867 GACCCGGTC
PvuII 361 CAGCTG CAG^CTG
1237 CAGCTG
SfaNI 192 GCATC GCATC(5/9)
722 GCATC
StuI 321 AGGCCT AGG^CCT
503 AGGCCT
TaqI 508 TCGA T^CGA
954 TCGA
Tth111I 547 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
867 GACCCGGTC
TthHB8I 508 TCGA T^CGA
954 TCGA
XcmI 972 CCAGGCCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1031 CCACTGTCACATTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsaWI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI Tsp45I TspRI
Van91I XhoII