(Circular) (Six-base) MAPSORT of: pFN21AB5902.seq Check: 1315 from: 1 to: 6280 pFN21AB5902 6280 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3008 3008 Size: 6280 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 1937 Size: 1686 4594 Acc65I G'GTAC_C Cuts at: 3601 3601 Size: 6280 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 4343 5727 5995 1632 Size: 928 1783 1384 268 1917 Fragments arranged by size: 1917 1783 1384 928 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 5179 6156 1203 Size: 3976 977 1327 Fragments arranged by size: 3976 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3048 1184 Size: 1864 4416 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2003 2035 2003 Size: 32 6248 AlwNI CAG_nnn'CTG Cuts at: 2023 2084 2600 3128 4023 5595 2023 Size: 61 516 528 895 1572 2708 Fragments arranged by size: 2708 1572 895 528 516 61 ApaI G_GGCC'C Cuts at: 2107 2286 2718 2107 Size: 179 432 5669 Fragments arranged by size: 5669 432 179 ApaLI G'TGCA_C Cuts at: 2456 4337 5493 2456 Size: 1881 1156 3243 Fragments arranged by size: 3243 1881 1156 ApoI r'AATT_y Cuts at: 1493 3589 3815 3967 6076 1493 Size: 2096 226 152 2109 1697 Fragments arranged by size: 2109 2096 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4834 160 Size: 872 3802 1606 Fragments arranged by size: 3802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 3551 3557 3563 3605 1870 Size: 73 1608 6 6 42 4545 Fragments arranged by size: 4545 1608 73 42 6 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3108 3141 3108 Size: 33 6247 BamHI G'GATC_C Cuts at: 3089 3610 3977 3089 Size: 521 367 5392 Fragments arranged by size: 5392 521 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 3305 3332 3601 5056 Size: 325 217 627 479 1039 27 269 1455 Cuts at: 5056 618 Size: 1842 Fragments arranged by size: 1842 1455 1039 627 479 325 269 217 27 BanII G_rGCy'C Cuts at: 1391 2107 2119 2286 2718 2796 3155 3328 3552 Size: 716 12 167 432 78 359 173 224 Cuts at: 3552 3558 3564 3599 1391 Size: 6 6 35 4072 Fragments arranged by size: 4072 716 432 359 224 173 167 78 35 12 6 6 BbeI G_GCGC'C Cuts at: 2270 2270 Size: 6280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3436 928 Size: 971 1537 3772 Fragments arranged by size: 3772 1537 971 BbvCI CC'TCA_GC Cuts at: 2085 2085 Size: 6280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4471 4505 1903 Size: 34 2534 34 3678 Fragments arranged by size: 3678 2534 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6275 6275 Size: 6280 BlpI GC'TnA_GC Cuts at: 2309 3697 2309 Size: 1388 4892 Bme1580I G_kGCm'C Cuts at: 1790 2107 2286 2460 2718 3310 3337 4341 5497 Size: 317 179 174 258 592 27 1004 1156 Cuts at: 5497 1790 Size: 2573 Fragments arranged by size: 2573 1156 1004 592 317 258 179 174 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6280 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 4011 4969 Size: 416 53 83 399 24 1146 1448 958 Cuts at: 4969 442 Size: 1753 Fragments arranged by size: 1753 1448 1146 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2057 2138 2810 2841 2955 3105 3218 Size: 804 14 81 672 31 114 150 113 Cuts at: 3218 3423 3530 4940 1239 Size: 205 107 1410 2579 Fragments arranged by size: 2579 1410 804 672 205 150 114 113 107 81 31 14 Bpu10I CC'TnA_GC Cuts at: 2085 2085 Size: 6280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4405 5270 5568 4405 Size: 865 298 5117 Fragments arranged by size: 5117 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4943 882 Size: 617 3444 2219 Fragments arranged by size: 3444 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2267 2433 4087 4469 Size: 53 83 186 936 734 166 1654 382 Cuts at: 4469 5104 275 Size: 635 1451 Fragments arranged by size: 1654 1451 936 734 635 382 186 166 83 53 BsaWI w'CCGG_w Cuts at: 2613 3520 3544 4713 5111 5385 5532 5943 2613 Size: 907 24 1169 398 274 147 411 2950 Fragments arranged by size: 2950 1169 907 411 398 274 147 24 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 3049 3079 3261 Size: 30 165 30 600 30 1016 30 182 Cuts at: 3261 3291 1178 Size: 30 4167 Fragments arranged by size: 4167 1016 600 182 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2263 2892 3190 3518 1186 Size: 1077 629 298 328 3948 Fragments arranged by size: 3948 1077 629 328 298 BseYI C'CCAG_C Cuts at: 2124 2416 2593 2987 3213 3225 3340 5483 5979 Size: 292 177 394 226 12 115 2143 496 Cuts at: 5979 2124 Size: 2425 Fragments arranged by size: 2425 2143 496 394 292 226 177 115 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5707 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2383 3730 4491 5519 1540 Size: 275 186 382 1347 761 1028 2301 Fragments arranged by size: 2301 1347 1028 761 382 275 186 BsiHKAI G_wGCw'C Cuts at: 2460 3155 4341 4426 5497 2460 Size: 695 1186 85 1071 3243 Fragments arranged by size: 3243 1186 1071 695 85 BsmI GAATG_Cn' Cuts at: 3791 3884 3791 Size: 93 6187 BsmBI CGTCTCn'nnnn_ Cuts at: 2761 2761 Size: 6280 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2119 2286 2460 2718 2796 3155 Size: 399 317 12 167 174 258 78 359 Cuts at: 3155 3310 3328 3337 3552 3558 3564 3599 4341 Size: 155 18 9 215 6 6 35 742 Cuts at: 4341 4426 5497 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 359 317 258 215 174 167 155 85 78 35 18 12 9 6 6 BspEI T'CCGG_A Cuts at: 2613 5943 2613 Size: 3330 2950 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 3008 3635 844 Size: 1972 192 627 3489 Fragments arranged by size: 3489 1972 627 192 BsrBI CCG'CTC Cuts at: 1394 1823 4167 1394 Size: 429 2344 3507 Fragments arranged by size: 3507 2344 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3242 4775 4949 65 Size: 1183 1994 1533 174 1396 Fragments arranged by size: 1994 1533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2382 2435 4924 5111 1700 Size: 110 572 53 2489 187 2869 Fragments arranged by size: 2869 2489 572 187 110 53 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 2425 1925 Size: 500 5780 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6280 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 3081 1184 Size: 1897 4383 BstXI CCAn_nnnn'nTGG Cuts at: 3317 3317 Size: 6280 BstYI r'GATC_y Cuts at: 1972 2158 2534 3089 3610 3977 4362 4379 5831 Size: 186 376 555 521 367 385 17 1452 Cuts at: 5831 5917 5929 6275 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 555 521 385 376 367 346 186 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2462 2462 Size: 6280 BtgI C'CryG_G Cuts at: 513 1065 2451 2703 513 Size: 552 1386 252 4090 Fragments arranged by size: 4090 1386 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 2886 3282 3796 4590 4610 2886 Size: 396 514 794 20 4556 Fragments arranged by size: 4556 794 514 396 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 3584 3584 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2380 3727 Size: 54 1425 50 275 18 403 147 1347 Cuts at: 3727 4616 6245 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1347 889 403 275 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2380 3727 1537 Size: 275 568 1347 4090 Fragments arranged by size: 4090 1347 568 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2170 2687 4210 1353 Size: 817 517 1523 3423 Fragments arranged by size: 3423 1523 817 517 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4867 5241 5387 387 Size: 1064 138 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3153 3153 Size: 6280 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2057 2138 2560 2810 2841 2955 Size: 393 411 14 81 422 250 31 114 Cuts at: 2955 3105 3218 3423 3530 4343 4940 5727 5995 Size: 150 113 205 107 813 597 787 268 Cuts at: 5995 1239 Size: 1524 Fragments arranged by size: 1524 813 787 597 422 411 393 268 250 205 150 114 113 107 81 31 14 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2910 2504 Size: 406 5874 EcoO109I rG'GnC_Cy Cuts at: 2089 2282 2289 2318 2715 2871 2915 2991 3188 Size: 193 7 29 397 156 44 76 197 Cuts at: 3188 2089 Size: 5181 Fragments arranged by size: 5181 397 197 193 156 76 44 29 7 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FspI TGC'GCA Cuts at: 2333 4786 2333 Size: 2453 3827 HaeII r_GCGC'y Cuts at: 1678 2270 5427 1678 Size: 592 3157 2531 Fragments arranged by size: 3157 2531 592 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2169 2201 2302 2334 2625 Size: 32 135 32 597 32 101 32 291 Cuts at: 2625 2657 3152 3184 3463 3495 4921 4953 4995 Size: 32 495 32 279 32 1426 32 42 Cuts at: 4995 5027 1373 Size: 32 2626 Fragments arranged by size: 2626 1426 597 495 291 279 135 101 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3624 3870 669 Size: 988 281 1686 246 3079 Fragments arranged by size: 3079 1686 988 281 246 HindIII A'AGCT_T Cuts at: 748 2674 748 Size: 1926 4354 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KasI G'GCGC_C Cuts at: 2266 2266 Size: 6280 KpnI G_GTAC'C Cuts at: 3605 3605 Size: 6280 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2574 5394 5578 1305 Size: 1269 2820 184 2007 Fragments arranged by size: 2820 2007 1269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4458 Fragments arranged by size: 4458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3059 4238 4597 4756 518 Size: 648 1893 1179 359 159 2042 Fragments arranged by size: 2042 1893 1179 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2222 2337 2368 2453 3244 3696 4373 5521 Size: 616 115 31 85 791 452 677 1148 Cuts at: 5521 5766 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 791 677 616 452 245 115 85 31 NaeI GCC'GGC Cuts at: 2384 2437 2384 Size: 53 6227 NarI GG'CG_CC Cuts at: 2267 2267 Size: 6280 NcoI C'CATG_G Cuts at: 513 1065 2703 513 Size: 552 1638 4090 Fragments arranged by size: 4090 1638 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NgoMIV G'CCGG_C Cuts at: 2382 2435 2382 Size: 53 6227 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 3638 5183 1132 Size: 97 2409 1545 2229 Fragments arranged by size: 2409 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2718 3094 1348 Size: 1370 376 4534 Fragments arranged by size: 4534 1370 376 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2210 2242 3128 1108 Size: 1102 32 886 4260 Fragments arranged by size: 4260 1102 886 32 PfoI T'CCnGG_A Cuts at: 2154 2320 2643 2154 Size: 166 323 5791 Fragments arranged by size: 5791 323 166 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2003 2035 3012 3044 4349 4381 5862 5894 2003 Size: 32 977 32 1305 32 1481 32 2389 Fragments arranged by size: 2389 1481 1305 977 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2318 2871 2991 3188 2089 Size: 229 553 120 197 5181 Fragments arranged by size: 5181 553 229 197 120 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PspOMI G'GGCC_C Cuts at: 2103 2282 2714 2103 Size: 179 432 5669 Fragments arranged by size: 5669 432 179 PstI C_TGCA'G Cuts at: 830 2043 2138 2355 3018 3632 830 Size: 1213 95 217 663 614 3478 Fragments arranged by size: 3478 1213 663 614 217 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 PvuII CAG'CTG Cuts at: 2368 3244 2368 Size: 876 5404 SacI G_AGCT'C Cuts at: 3155 3155 Size: 6280 SacII CC_GC'GG Cuts at: 2454 2454 Size: 6280 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SanDI GG'GwC_CC Cuts at: 2318 2871 2318 Size: 553 5727 SapI GCTCTTCn'nnn_ Cuts at: 2170 2170 Size: 6280 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SfcI C'TryA_G Cuts at: 826 1045 2039 2134 2351 2737 3014 3628 4763 Size: 219 994 95 217 386 277 614 1135 Cuts at: 4763 5444 5635 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 994 681 614 386 277 219 217 191 95 SfoI GGC'GCC Cuts at: 2268 2268 Size: 6280 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 2435 1700 Size: 735 5545 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4384 5285 5547 820 Size: 197 926 2441 901 262 1553 Fragments arranged by size: 2441 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 4204 5 Size: 47 4152 2081 Fragments arranged by size: 4152 2081 47 StuI AGG'CCT Cuts at: 1517 2328 2510 1517 Size: 811 182 5287 Fragments arranged by size: 5287 811 182 StyI C'CwwG_G Cuts at: 513 1065 2703 3191 3230 3370 3719 513 Size: 552 1638 488 39 140 349 3074 Fragments arranged by size: 3074 1638 552 488 349 140 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 4307 4324 4477 4662 5107 325 Size: 1255 461 2266 17 153 185 445 1498 Fragments arranged by size: 2266 1498 1255 461 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4526 6238 Size: 274 80 33 51 1443 1302 1247 1712 Cuts at: 6238 96 Size: 138 Fragments arranged by size: 1712 1443 1302 1247 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3049 3081 3261 3293 6240 6272 3049 Size: 32 180 32 2947 32 3057 Fragments arranged by size: 3057 2947 180 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2555 2875 1316 Size: 1239 320 4721 Fragments arranged by size: 4721 1239 320 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2984 3043 2984 Size: 59 6221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 6280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI