Restriction map for
pFN21AB5902
(Linear) (Six-base) MAPSORT of: KIBB5902.seq Check: 7609 from: 1 to: 1575
KIBB5902.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1003 1575
Size: 1003 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1575
Size: 555 1020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1043 1575
Size: 1043 532
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 1575
Size: 30 1545
AlwNI CAG_nnn'CTG
Cuts at: 0 18 79 595 1123 1575
Size: 18 61 516 528 452
Fragments arranged by size:
528 516 452 61 18
ApaI G_GGCC'C
Cuts at: 0 102 281 713 1575
Size: 102 179 432 862
Fragments arranged by size:
862 432 179 102
ApaLI G'TGCA_C
Cuts at: 0 451 1575
Size: 451 1124
AvaI C'yCGr_G
Cuts at: 0 1546 1552 1558 1575
Size: 1546 6 6 17
Fragments arranged by size:
1546 17 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1103 1136 1575
Size: 1103 33 439
Fragments arranged by size:
1103 439 33
BamHI G'GATC_C
Cuts at: 0 1084 1575
Size: 1084 491
BanI G'GyrC_C
Cuts at: 0 261 1300 1327 1575
Size: 261 1039 27 248
Fragments arranged by size:
1039 261 248 27
BanII G_rGCy'C
Cuts at: 0 102 114 281 713 791 1150 1323 1547
Size: 102 12 167 432 78 359 173 224
Cuts at: 1547 1553 1559 1575
Size: 6 6 16
Fragments arranged by size:
432 359 224 173 167 102 78 16
12 6 6
BbeI G_GCGC'C
Cuts at: 0 265 1575
Size: 265 1310
BbsI GAAGACnn'nnnn_
Cuts at: 0 1431 1575
Size: 1431 144
BbvCI CC'TCA_GC
Cuts at: 0 80 1575
Size: 80 1495
BlpI GC'TnA_GC
Cuts at: 0 304 1575
Size: 304 1271
Bme1580I G_kGCm'C
Cuts at: 0 102 281 455 713 1305 1332 1575
Size: 102 179 174 258 592 27 243
Fragments arranged by size:
592 258 243 179 174 102 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 1575
Size: 558 1017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 52 133 805 836 950 1100 1213
Size: 38 14 81 672 31 114 150 113
Cuts at: 1213 1418 1525 1575
Size: 205 107 50
Fragments arranged by size:
672 205 150 114 113 107 81 50
38 31 14
Bpu10I CC'TnA_GC
Cuts at: 0 80 1575
Size: 80 1495
BsaHI Gr'CG_yC
Cuts at: 0 262 428 1575
Size: 262 166 1147
Fragments arranged by size:
1147 262 166
BsaWI w'CCGG_w
Cuts at: 0 608 1515 1539 1575
Size: 608 907 24 36
Fragments arranged by size:
907 608 36 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 1044 1074 1256 1286 1575
Size: 28 1016 30 182 30 289
Fragments arranged by size:
1016 289 182 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 887 1185 1513 1575
Size: 258 629 298 328 62
Fragments arranged by size:
629 328 298 258 62
BseYI C'CCAG_C
Cuts at: 0 119 411 588 982 1208 1220 1335 1575
Size: 119 292 177 394 226 12 115 240
Fragments arranged by size:
394 292 240 226 177 119 115 12
BsiEI CG_ry'CG
Cuts at: 0 378 1575
Size: 378 1197
BsiHKAI G_wGCw'C
Cuts at: 0 455 1150 1575
Size: 455 695 425
Fragments arranged by size:
695 455 425
BsmBI CGTCTCn'nnnn_
Cuts at: 0 756 1575
Size: 756 819
Bsp1286I G_dGCh'C
Cuts at: 0 102 114 281 455 713 791 1150 1305
Size: 102 12 167 174 258 78 359 155
Cuts at: 1305 1323 1332 1547 1553 1559 1575
Size: 18 9 215 6 6 16
Fragments arranged by size:
359 258 215 174 167 155 102 78
18 16 12 9 6 6
BspEI T'CCGG_A
Cuts at: 0 608 1575
Size: 608 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 811 1003 1575
Size: 811 192 572
Fragments arranged by size:
811 572 192
BsrDI GCAATG_nn'
Cuts at: 0 1237 1575
Size: 1237 338
BsrFI r'CCGG_y
Cuts at: 0 377 430 1575
Size: 377 53 1145
Fragments arranged by size:
1145 377 53
BssHII G'CGCG_C
Cuts at: 0 420 1575
Size: 420 1155
BstEII G'GTnAC_C
Cuts at: 0 1076 1575
Size: 1076 499
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1312 1575
Size: 1312 263
BstYI r'GATC_y
Cuts at: 0 153 529 1084 1575
Size: 153 376 555 491
Fragments arranged by size:
555 491 376 153
Bsu36I CC'TnA_GG
Cuts at: 0 457 1575
Size: 457 1118
BtgI C'CryG_G
Cuts at: 0 446 698 1575
Size: 446 252 877
Fragments arranged by size:
877 446 252
BtsI GCAGTG_nn'
Cuts at: 0 881 1277 1575
Size: 881 396 298
Fragments arranged by size:
881 396 298
EaeI y'GGCC_r
Cuts at: 0 228 375 1575
Size: 228 147 1200
Fragments arranged by size:
1200 228 147
EagI C'GGCC_G
Cuts at: 0 375 1575
Size: 375 1200
EarI CTCTTCn'nnn_
Cuts at: 0 165 682 1575
Size: 165 517 893
Fragments arranged by size:
893 517 165
EcoICRI GAG'CTC
Cuts at: 0 1148 1575
Size: 1148 427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 52 133 555 805 836 950 1100
Size: 38 14 81 422 250 31 114 150
Cuts at: 1100 1213 1418 1525 1575
Size: 113 205 107 50
Fragments arranged by size:
422 250 205 150 114 113 107 81
50 38 31 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 905 1575
Size: 499 406 670
Fragments arranged by size:
670 499 406
EcoO109I rG'GnC_Cy
Cuts at: 0 84 277 284 313 710 866 910 986
Size: 84 193 7 29 397 156 44 76
Cuts at: 986 1183 1575
Size: 197 392
Fragments arranged by size:
397 392 197 193 156 84 76 44
29 7
FspI TGC'GCA
Cuts at: 0 328 1575
Size: 328 1247
HaeII r_GCGC'y
Cuts at: 0 265 1575
Size: 265 1310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 164 196 297 329 620 652 1147 1179
Size: 164 32 101 32 291 32 495 32
Cuts at: 1179 1458 1490 1575
Size: 279 32 85
Fragments arranged by size:
495 291 279 164 101 85 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 669 1575
Size: 669 906
KasI G'GCGC_C
Cuts at: 0 261 1575
Size: 261 1314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1575
Size: 569 1006
MslI CAynn'nnrTG
Cuts at: 0 1054 1575
Size: 1054 521
MspA1I CmG'CkG
Cuts at: 0 217 332 363 448 1239 1575
Size: 217 115 31 85 791 336
Fragments arranged by size:
791 336 217 115 85 31
NaeI GCC'GGC
Cuts at: 0 379 432 1575
Size: 379 53 1143
Fragments arranged by size:
1143 379 53
NarI GG'CG_CC
Cuts at: 0 262 1575
Size: 262 1313
NcoI C'CATG_G
Cuts at: 0 698 1575
Size: 698 877
NgoMIV G'CCGG_C
Cuts at: 0 377 430 1575
Size: 377 53 1145
Fragments arranged by size:
1145 377 53
PasI CC'CwG_GG
Cuts at: 0 713 1089 1575
Size: 713 376 486
Fragments arranged by size:
713 486 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 205 237 1123 1575
Size: 205 32 886 452
Fragments arranged by size:
886 452 205 32
PfoI T'CCnGG_A
Cuts at: 0 149 315 638 1575
Size: 149 166 323 937
Fragments arranged by size:
937 323 166 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1007 1039 1575
Size: 30 977 32 536
Fragments arranged by size:
977 536 32 30
PpuMI rG'GwC_Cy
Cuts at: 0 84 313 866 986 1183 1575
Size: 84 229 553 120 197 392
Fragments arranged by size:
553 392 229 197 120 84
PspOMI G'GGCC_C
Cuts at: 0 98 277 709 1575
Size: 98 179 432 866
Fragments arranged by size:
866 432 179 98
PstI C_TGCA'G
Cuts at: 0 38 133 350 1013 1575
Size: 38 95 217 663 562
Fragments arranged by size:
663 562 217 95 38
PvuII CAG'CTG
Cuts at: 0 363 1239 1575
Size: 363 876 336
Fragments arranged by size:
876 363 336
SacI G_AGCT'C
Cuts at: 0 1150 1575
Size: 1150 425
SacII CC_GC'GG
Cuts at: 0 449 1575
Size: 449 1126
SanDI GG'GwC_CC
Cuts at: 0 313 866 1575
Size: 313 553 709
Fragments arranged by size:
709 553 313
SapI GCTCTTCn'nnn_
Cuts at: 0 165 1575
Size: 165 1410
SfcI C'TryA_G
Cuts at: 0 34 129 346 732 1009 1575
Size: 34 95 217 386 277 566
Fragments arranged by size:
566 386 277 217 95 34
SfoI GGC'GCC
Cuts at: 0 263 1575
Size: 263 1312
SgrAI Cr'CCGG_yG
Cuts at: 0 430 1575
Size: 430 1145
StuI AGG'CCT
Cuts at: 0 323 505 1575
Size: 323 182 1070
Fragments arranged by size:
1070 323 182
StyI C'CwwG_G
Cuts at: 0 698 1186 1225 1365 1575
Size: 698 488 39 140 210
Fragments arranged by size:
698 488 210 140 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 1575
Size: 36 1539
TatI w'GTAC_w
Cuts at: 0 1274 1575
Size: 1274 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 1256 1288 1575
Size: 1044 32 180 32 287
Fragments arranged by size:
1044 287 180 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 550 870 1575
Size: 550 320 705
Fragments arranged by size:
705 550 320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 979 1038 1575
Size: 979 59 537
Fragments arranged by size:
979 537 59
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApaLI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BlpI
Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HindIII KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SapI SfcI
SfoI SgrAI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApoI AscI AseI AsiSI AvrII BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsgI BsiWI BsmI BspHI
BsrBI BsrGI BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI
FseI FspAI HincII HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SexAI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmaI XmnI ZraI