(Circular) (Six-base) MAPSORT of: pFN21AB5901.seq Check: 9421 from: 1 to: 6205 pFN21AB5901 6205 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 3548 1937 Size: 1080 531 4594 Fragments arranged by size: 4594 1080 531 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 4268 5652 5920 1632 Size: 1436 1200 1384 268 1917 Fragments arranged by size: 1917 1436 1384 1200 268 AflII C'TTAA_G Cuts at: 820 1017 2873 820 Size: 197 1856 4152 Fragments arranged by size: 4152 1856 197 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 2744 4934 2744 Size: 2190 4015 AleI CACnn'nnGTG Cuts at: 1166 2650 3062 1166 Size: 1484 412 4309 Fragments arranged by size: 4309 1484 412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2768 3948 5520 2768 Size: 1180 1572 3453 Fragments arranged by size: 3453 1572 1180 ApaI G_GGCC'C Cuts at: 3041 3041 Size: 6205 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2066 2705 3133 3145 3463 3514 3740 3892 Size: 573 639 428 12 318 51 226 152 Cuts at: 3892 6001 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 639 573 428 318 226 152 51 12 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 3530 1870 Size: 73 1587 4545 Fragments arranged by size: 4545 1587 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3483 3516 3483 Size: 33 6172 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 3526 4981 618 Size: 325 217 627 1619 120 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 120 BanII G_rGCy'C Cuts at: 1391 2024 3041 3524 1391 Size: 633 1017 483 4072 Fragments arranged by size: 4072 1017 633 483 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5234 BbvCI CC'TCA_GC Cuts at: 2474 2474 Size: 6205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2872 3511 4097 5307 2872 Size: 639 586 1210 3770 Fragments arranged by size: 3770 1210 639 586 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BfrBI ATG'CAT Cuts at: 2544 2544 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 3041 3411 4266 5422 1790 Size: 1251 370 855 1156 2573 Fragments arranged by size: 2573 1251 1156 855 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3047 3936 4894 Size: 416 53 83 399 24 1630 889 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1753 1630 958 889 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4865 1239 Size: 3626 2579 Bpu10I CC'TnA_GC Cuts at: 2474 2474 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2892 4330 5195 5493 2892 Size: 1438 865 298 3604 Fragments arranged by size: 3604 1438 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5980 Fragments arranged by size: 5980 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 1186 Size: 862 5343 BseYI C'CCAG_C Cuts at: 5408 5904 5408 Size: 496 5709 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3390 1163 Size: 573 1654 3978 Fragments arranged by size: 3978 1654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2762 4266 4351 5422 2762 Size: 1504 85 1071 3545 Fragments arranged by size: 3545 1504 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3716 3809 3233 Size: 483 93 5629 Fragments arranged by size: 5629 483 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2762 3041 3411 3524 4266 4351 Size: 399 234 738 279 370 113 742 85 Cuts at: 4351 5422 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 738 399 370 279 234 113 85 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2077 4092 1394 Size: 429 254 2015 3507 Fragments arranged by size: 3507 2015 429 254 BsrDI GCAATG_nn' Cuts at: 65 1248 2544 3117 3361 4700 4874 65 Size: 1183 1296 573 244 1339 174 1396 Fragments arranged by size: 1396 1339 1296 1183 573 244 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3186 96 Size: 3090 3115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 2168 794 Size: 1374 4831 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstYI r'GATC_y Cuts at: 1972 3535 3902 4287 4304 5756 5842 5854 6200 Size: 1563 367 385 17 1452 86 12 346 Cuts at: 6200 1972 Size: 1977 Fragments arranged by size: 1977 1563 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3018 3018 Size: 6205 Bsu36I CC'TnA_GG Cuts at: 2551 2551 Size: 6205 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 3353 513 Size: 552 939 24 1325 3365 Fragments arranged by size: 3365 1325 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 2286 487 Size: 683 56 814 246 4406 Fragments arranged by size: 4406 814 683 246 56 BtsI GCAGTG_nn' Cuts at: 2242 2657 3373 3721 4515 4535 2242 Size: 415 716 348 794 20 3912 Fragments arranged by size: 3912 794 716 415 348 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 2124 3509 2124 Size: 1385 4820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3356 3652 4541 Size: 54 1425 50 275 18 1526 296 889 Cuts at: 4541 6170 8 Size: 1629 43 Fragments arranged by size: 1629 1526 1425 889 296 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4135 1353 Size: 2782 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3068 4268 4865 5652 5920 1239 Size: 393 1436 1200 597 787 268 1524 Fragments arranged by size: 1524 1436 1200 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3038 3038 Size: 6205 EcoRI G'AATT_C Cuts at: 2705 3514 3892 2705 Size: 809 378 5018 Fragments arranged by size: 5018 809 378 FspI TGC'GCA Cuts at: 2046 4711 2046 Size: 2665 3540 HaeII r_GCGC'y Cuts at: 1678 2982 5352 1678 Size: 1304 2370 2531 Fragments arranged by size: 2531 2370 1304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 2730 2762 2828 Size: 32 135 32 1103 32 23 32 66 Cuts at: 2828 2860 4846 4878 4920 4952 1373 Size: 32 1986 32 42 32 2626 Fragments arranged by size: 2626 1986 1103 135 66 42 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3227 5319 5503 1305 Size: 1922 2092 184 2007 Fragments arranged by size: 2092 2007 1922 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4383 Fragments arranged by size: 4383 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2650 2859 3062 3366 4163 4522 Size: 648 1299 185 209 203 304 797 359 Cuts at: 4522 4681 518 Size: 159 2042 Fragments arranged by size: 2042 1299 797 648 359 304 209 203 185 159 MspA1I CmG'CkG Cuts at: 1606 2030 3355 3621 4298 5446 5691 1606 Size: 424 1325 266 677 1148 245 2120 Fragments arranged by size: 2120 1325 1148 677 424 266 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 NdeI CA'TA_TG Cuts at: 387 2182 387 Size: 1795 4410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NsiI A_TGCA'T Cuts at: 2546 2546 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2051 2618 3563 5108 1132 Size: 97 822 567 945 1545 2229 Fragments arranged by size: 2229 1545 945 822 567 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2060 1108 Size: 952 5253 PfoI T'CCnGG_A Cuts at: 2984 2984 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 3037 3037 Size: 6205 PstI C_TGCA'G Cuts at: 830 3557 830 Size: 2727 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SacII CC_GC'GG Cuts at: 2031 3356 2031 Size: 1325 4880 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 2148 4453 6165 2148 Size: 2305 1712 2188 Fragments arranged by size: 2305 2188 1712 SfcI C'TryA_G Cuts at: 826 1045 3486 3553 4688 5369 5560 826 Size: 219 2441 67 1135 681 191 1471 Fragments arranged by size: 2441 1471 1135 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2873 2907 4309 5210 5472 820 Size: 197 926 930 34 1402 901 262 1553 Fragments arranged by size: 1553 1402 930 926 901 262 197 34 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 2051 3563 1132 Size: 919 1512 3774 Fragments arranged by size: 3774 1512 919 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2004 3644 513 Size: 552 939 1640 3074 Fragments arranged by size: 3074 1640 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4232 4249 4402 4587 5032 325 Size: 1255 2652 17 153 185 445 1498 Fragments arranged by size: 2652 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2146 3186 4451 Size: 274 80 33 51 1443 169 1040 1265 Cuts at: 4451 6163 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1265 1040 274 169 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2056 2088 2547 2579 2926 2958 6165 6197 2056 Size: 32 459 32 347 32 3207 32 2064 Fragments arranged by size: 3207 2064 459 347 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI