(Circular) (Six-base) MAPSORT of: pFN21AB5891.seq Check: 5452 from: 1 to: 6199 pFN21AB5891 6199 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2971 2971 Size: 6199 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 3542 1937 Size: 283 1322 4594 Fragments arranged by size: 4594 1322 283 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2287 4262 5646 5914 1632 Size: 655 1975 1384 268 1917 Fragments arranged by size: 1975 1917 1384 655 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 2597 5098 6075 1203 Size: 1394 2501 977 1327 Fragments arranged by size: 2501 1394 1327 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 3490 4928 3490 Size: 1438 4761 AleI CACnn'nnGTG Cuts at: 1166 2687 1166 Size: 1521 4678 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AlwNI CAG_nnn'CTG Cuts at: 2360 2765 3122 3942 5514 2360 Size: 405 357 820 1572 3045 Fragments arranged by size: 3045 1572 820 405 357 ApaI G_GGCC'C Cuts at: 3210 3210 Size: 6199 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2447 2636 2822 3473 3508 3734 3886 5995 Size: 954 189 186 651 35 226 152 2109 Cuts at: 5995 1493 Size: 1697 Fragments arranged by size: 2109 1697 954 651 226 189 186 152 35 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2945 3524 1870 Size: 73 250 752 579 4545 Fragments arranged by size: 4545 752 579 250 73 BamHI G'GATC_C Cuts at: 2251 3529 3896 2251 Size: 1278 367 4554 Fragments arranged by size: 4554 1278 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 3520 4975 618 Size: 325 217 627 250 1483 1455 1842 Fragments arranged by size: 1842 1483 1455 627 325 250 217 BanII G_rGCy'C Cuts at: 1391 2166 3210 3518 1391 Size: 775 1044 308 4072 Fragments arranged by size: 4072 1044 775 308 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4390 4424 1903 Size: 34 50 34 2369 34 3678 Fragments arranged by size: 3678 2369 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2685 4091 5301 2685 Size: 1406 1210 3583 Fragments arranged by size: 3583 1406 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 3210 4260 5416 1790 Size: 1420 1050 1156 2573 Fragments arranged by size: 2573 1420 1156 1050 BmgBI CAC'GTC Cuts at: 1318 3409 1318 Size: 2091 4108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2348 2828 3930 Size: 416 53 83 399 24 931 480 1102 Cuts at: 3930 4888 442 Size: 958 1753 Fragments arranged by size: 1753 1102 958 931 480 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3018 4859 1239 Size: 1779 1841 2579 Fragments arranged by size: 2579 1841 1779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4324 5189 5487 4324 Size: 865 298 5036 Fragments arranged by size: 5036 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3036 4862 882 Size: 617 1537 1826 2219 Fragments arranged by size: 2219 1826 1537 617 BsaAI yAC'GTr Cuts at: 493 1204 2585 2600 3990 493 Size: 711 1381 15 1390 2702 Fragments arranged by size: 2702 1390 1381 711 15 BsaBI GATnn'nnATC Cuts at: 2996 6193 2996 Size: 3197 3002 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3041 4006 4388 5023 Size: 53 83 186 936 1508 965 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1508 1451 965 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2382 2799 2904 2907 3093 3096 3168 3171 Size: 1196 417 105 3 186 3 72 3 Cuts at: 3171 3180 3183 3186 3189 3204 3380 3457 1186 Size: 9 3 3 3 15 176 77 3928 Fragments arranged by size: 3928 1196 417 186 176 105 77 72 15 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2122 2659 3156 5402 5898 2122 Size: 537 497 2246 496 2423 Fragments arranged by size: 2423 2246 537 497 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3252 1163 Size: 573 1516 4110 Fragments arranged by size: 4110 1516 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 3649 4410 5438 1540 Size: 275 186 223 1425 761 1028 2301 Fragments arranged by size: 2301 1425 1028 761 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2166 3175 4260 4345 5416 2166 Size: 1009 1085 85 1071 2949 Fragments arranged by size: 2949 1085 1071 1009 85 BsmI GAATG_Cn' Cuts at: 2087 3710 3803 2087 Size: 1623 93 4483 Fragments arranged by size: 4483 1623 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 3175 3210 3518 4260 4345 5416 Size: 399 376 1009 35 308 742 85 1071 Cuts at: 5416 1391 Size: 2174 Fragments arranged by size: 2174 1071 1009 742 399 376 308 85 35 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 2517 4088 5818 2517 Size: 1571 1730 2898 Fragments arranged by size: 2898 1730 1571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2971 3330 3554 844 Size: 2127 359 224 3489 Fragments arranged by size: 3489 2127 359 224 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3409 3432 4694 4868 65 Size: 1183 2161 23 1262 174 1396 Fragments arranged by size: 2161 1396 1262 1183 174 23 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6199 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstYI r'GATC_y Cuts at: 1972 2251 2852 2876 3529 3896 4281 4298 5750 Size: 279 601 24 653 367 385 17 1452 Cuts at: 5750 5836 5848 6194 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 653 601 385 367 346 279 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5647 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3321 487 Size: 683 56 2095 3365 Fragments arranged by size: 3365 2095 683 56 BtsI GCAGTG_nn' Cuts at: 2273 3715 4509 4529 2273 Size: 1442 794 20 3943 Fragments arranged by size: 3943 1442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2667 2702 3132 3167 529 Size: 35 2103 35 430 35 3561 Fragments arranged by size: 3561 2103 430 35 35 35 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3238 3411 3646 Size: 54 1425 50 275 18 1408 173 235 Cuts at: 3646 4535 6164 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1408 889 275 235 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 2601 4129 1353 Size: 1169 79 1528 3423 Fragments arranged by size: 3423 1528 1169 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2733 2961 2970 4786 5160 5306 Size: 1064 138 1144 228 9 1816 374 146 Cuts at: 5306 387 Size: 1280 Fragments arranged by size: 1816 1280 1144 1064 374 228 146 138 9 EcoICRI GAG'CTC Cuts at: 2164 2164 Size: 6199 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 3018 4262 4859 5646 5914 1239 Size: 393 655 731 1244 597 787 268 1524 Fragments arranged by size: 1524 1244 787 731 655 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2919 2919 Size: 6199 EcoO109I rG'GnC_Cy Cuts at: 2936 3206 3207 2936 Size: 270 1 5928 Fragments arranged by size: 5928 270 1 EcoRI G'AATT_C Cuts at: 2822 3508 3886 2822 Size: 686 378 5135 Fragments arranged by size: 5135 686 378 EcoRV GAT'ATC Cuts at: 2515 2515 Size: 6199 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3307 3307 Size: 6199 FspI TGC'GCA Cuts at: 3356 4705 3356 Size: 1349 4850 HaeII r_GCGC'y Cuts at: 1678 5346 1678 Size: 3668 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 3452 3476 3484 Size: 32 135 32 758 32 1090 24 8 Cuts at: 3484 3508 4840 4872 4914 4946 1373 Size: 24 1332 32 42 32 2626 Fragments arranged by size: 2626 1332 1090 758 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2221 3543 3789 669 Size: 988 281 283 1322 246 3079 Fragments arranged by size: 3079 1322 988 283 281 246 HindIII A'AGCT_T Cuts at: 748 2330 748 Size: 1582 4617 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2140 5313 5497 1305 Size: 835 3173 184 2007 Fragments arranged by size: 3173 2007 835 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3240 3413 10 Size: 54 1425 343 1408 173 2796 Fragments arranged by size: 2796 1425 1408 343 173 54 MslI CAynn'nnrTG Cuts at: 518 1166 2687 4157 4516 4675 518 Size: 648 1521 1470 359 159 2042 Fragments arranged by size: 2042 1521 1470 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2248 3122 3352 3373 3615 4292 5440 Size: 516 126 874 230 21 242 677 1148 Cuts at: 5440 5685 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 874 677 516 245 242 230 126 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5647 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 3557 5102 1132 Size: 97 2328 1545 2229 Fragments arranged by size: 2328 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 3206 3206 Size: 6199 PstI C_TGCA'G Cuts at: 830 2502 3551 830 Size: 1672 1049 3478 Fragments arranged by size: 3478 1672 1049 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 PvuII CAG'CTG Cuts at: 2122 2248 3122 3352 2122 Size: 126 874 230 4969 Fragments arranged by size: 4969 874 230 126 SacI G_AGCT'C Cuts at: 2166 2166 Size: 6199 SalI G'TCGA_C Cuts at: 1936 2219 3541 1936 Size: 283 1322 4594 Fragments arranged by size: 4594 1322 283 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SfcI C'TryA_G Cuts at: 826 1045 2228 2498 3018 3547 4682 5363 5554 Size: 219 1183 270 520 529 1135 681 191 Cuts at: 5554 826 Size: 1471 Fragments arranged by size: 1471 1183 1135 681 529 520 270 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 2947 3526 1872 Size: 1075 579 4545 Fragments arranged by size: 4545 1075 579 SmlI C'TyrA_G Cuts at: 820 1017 1943 4303 5204 5466 820 Size: 197 926 2360 901 262 1553 Fragments arranged by size: 2360 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 2033 2256 2508 3638 513 Size: 552 968 223 252 1130 3074 Fragments arranged by size: 3074 1130 968 552 252 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 2695 4226 4243 4396 4581 5026 Size: 1255 658 457 1531 17 153 185 445 Cuts at: 5026 325 Size: 1498 Fragments arranged by size: 1531 1498 1255 658 457 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3444 4445 6157 Size: 274 80 33 51 1443 1467 1001 1712 Cuts at: 6157 96 Size: 138 Fragments arranged by size: 1712 1467 1443 1001 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2656 2852 2098 Size: 558 196 5445 Fragments arranged by size: 5445 558 196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 2945 3524 1870 Size: 1075 579 4545 Fragments arranged by size: 4545 1075 579 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI