(Circular) (Six-base) MAPSORT of: pFN21AB5883.seq Check: 5403 from: 1 to: 6106 pFN21AB5883 6106 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 4169 5553 5821 1632 Size: 455 2082 1384 268 1917 Fragments arranged by size: 2082 1917 1384 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 2342 4835 2342 Size: 2493 3613 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3383 3415 3383 Size: 32 6074 AlwNI CAG_nnn'CTG Cuts at: 2833 3079 3849 5421 2833 Size: 246 770 1572 3518 Fragments arranged by size: 3518 1572 770 246 ApaI G_GGCC'C Cuts at: 2025 2308 2725 3068 2025 Size: 283 417 343 5063 Fragments arranged by size: 5063 417 343 283 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2357 3397 3415 3641 3793 5902 1493 Size: 864 1040 18 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 864 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2324 3431 1870 Size: 73 381 1107 4545 Fragments arranged by size: 4545 1107 381 73 AvrII C'CTAG_G Cuts at: 2623 2623 Size: 6106 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3427 4882 618 Size: 325 217 627 1640 1455 1842 Fragments arranged by size: 1842 1640 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2308 2725 2937 3068 3425 1391 Size: 634 283 417 212 131 357 4072 Fragments arranged by size: 4072 634 417 357 283 212 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2447 3998 5208 2447 Size: 1551 1210 3345 Fragments arranged by size: 3345 1551 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2230 2853 4717 Size: 107 122 71 631 605 558 623 1864 Cuts at: 4717 136 Size: 1525 Fragments arranged by size: 1864 1525 631 623 605 558 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2025 2157 2308 2622 2725 2801 3068 4167 Size: 235 132 151 314 103 76 267 1099 Cuts at: 4167 5323 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1099 314 267 235 151 132 103 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2728 2763 3837 Size: 416 53 83 399 24 1311 35 1074 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1311 1074 958 416 399 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3316 3316 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2265 2351 3077 4766 1239 Size: 1026 86 726 1689 2579 Fragments arranged by size: 2579 1689 1026 726 86 Bpu10I CC'TnA_GC Cuts at: 3186 3186 Size: 6106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3140 4231 5096 5394 3140 Size: 1091 865 298 3852 Fragments arranged by size: 3852 1091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2458 3304 4769 882 Size: 617 959 846 1465 2219 Fragments arranged by size: 2219 1465 959 846 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2779 4539 4937 5211 5358 5769 2779 Size: 1760 398 274 147 411 3116 Fragments arranged by size: 3116 1760 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5881 Fragments arranged by size: 5881 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2358 2987 3004 3013 1186 Size: 1172 629 17 9 4279 Fragments arranged by size: 4279 1172 629 17 9 BseYI C'CCAG_C Cuts at: 2079 2209 2233 2397 5309 5805 2079 Size: 130 24 164 2912 496 2380 Fragments arranged by size: 2912 2380 496 164 130 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2663 3114 1163 Size: 573 927 451 4155 Fragments arranged by size: 4155 927 573 451 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 3265 4167 4252 5323 2703 Size: 562 902 85 1071 3486 Fragments arranged by size: 3486 1071 902 562 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2157 2308 2622 2703 2725 2801 Size: 399 235 132 151 314 81 22 76 Cuts at: 2801 2937 3068 3265 3425 4167 4252 5323 1391 Size: 136 131 197 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 314 235 197 160 151 136 132 131 85 81 76 22 BspEI T'CCGG_A Cuts at: 2779 5769 2779 Size: 2990 3116 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 844 Size: 2617 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2991 4601 4775 65 Size: 1183 1743 1610 174 1396 Fragments arranged by size: 1743 1610 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 2174 794 Size: 1380 4726 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 3295 3295 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2551 3106 3436 3803 4188 4205 5657 5743 Size: 579 555 330 367 385 17 1452 86 Cuts at: 5743 5755 6101 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 579 555 385 367 346 330 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 4603 Fragments arranged by size: 4603 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 3622 4416 4436 3622 Size: 794 20 5292 Fragments arranged by size: 5292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2910 3553 Size: 54 1425 50 275 18 225 855 643 Cuts at: 3553 4442 6071 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 855 643 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2185 2233 2415 3167 4036 1353 Size: 832 48 182 752 869 3423 Fragments arranged by size: 3423 869 832 752 182 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2265 2351 3077 4169 4766 5553 Size: 393 455 178 86 726 1092 597 787 Cuts at: 5553 5821 1239 Size: 268 1524 Fragments arranged by size: 1524 1092 787 726 597 455 393 268 178 86 EcoO109I rG'GnC_Cy Cuts at: 2021 2071 2104 2222 2266 2344 2709 2722 2895 Size: 50 33 118 44 78 365 13 173 Cuts at: 2895 2963 3065 2021 Size: 68 102 5062 Fragments arranged by size: 5062 365 173 118 102 78 68 50 44 33 13 EcoRI G'AATT_C Cuts at: 2357 3397 3415 3793 2357 Size: 1040 18 378 4670 Fragments arranged by size: 4670 1040 378 18 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 5253 1678 Size: 3575 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2328 2360 2536 2568 3052 Size: 32 135 32 756 32 176 32 484 Cuts at: 3052 3084 4747 4779 4821 4853 1373 Size: 32 1663 32 42 32 2626 Fragments arranged by size: 2626 1663 756 484 176 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3100 3450 3696 669 Size: 988 281 1162 350 246 3079 Fragments arranged by size: 3079 1162 988 350 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2910 3246 5220 5404 1305 Size: 1605 336 1974 184 2007 Fragments arranged by size: 2007 1974 1605 336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2912 10 Size: 54 1425 343 225 855 3204 Fragments arranged by size: 3204 1425 855 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2794 2814 4064 4423 4582 518 Size: 648 1628 20 1250 359 159 2042 Fragments arranged by size: 2042 1628 1250 648 359 159 20 MspA1I CmG'CkG Cuts at: 1606 3522 4199 5347 5592 1606 Size: 1916 677 1148 245 2120 Fragments arranged by size: 2120 1916 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4615 Fragments arranged by size: 4615 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2177 3464 5009 1132 Size: 97 948 1287 1545 2229 Fragments arranged by size: 2229 1545 1287 948 97 PasI CC'CwG_GG Cuts at: 1348 3197 1348 Size: 1849 4257 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2262 2753 3197 1108 Size: 1154 491 444 4017 Fragments arranged by size: 4017 1154 491 444 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2266 2344 2895 2963 2104 Size: 162 78 551 68 5247 Fragments arranged by size: 5247 551 162 78 68 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 2021 2304 2721 3064 2021 Size: 283 417 343 5063 Fragments arranged by size: 5063 417 343 283 PstI C_TGCA'G Cuts at: 830 2051 3458 830 Size: 1221 1407 3478 Fragments arranged by size: 3478 1407 1221 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 2233 2233 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2047 2448 3270 3454 4589 5270 5461 Size: 219 1002 401 822 184 1135 681 191 Cuts at: 5461 826 Size: 1471 Fragments arranged by size: 1471 1135 1002 822 681 401 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3119 4210 5111 5373 820 Size: 197 926 1176 1091 901 262 1553 Fragments arranged by size: 1553 1176 1091 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2177 3464 1132 Size: 1045 1287 3774 Fragments arranged by size: 3774 1287 1045 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2004 2099 2623 3288 3545 513 Size: 552 939 95 524 665 257 3074 Fragments arranged by size: 3074 939 665 552 524 257 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4352 6064 96 Size: 274 80 33 51 1443 2375 1712 138 Fragments arranged by size: 2375 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2510 2326 Size: 184 5922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI