Restriction map for
pFN21AB5766
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 1102 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2002 GAGCTC G[AT]GC[AT]^C
AvaI 1061 CTCGGG C^[CT]CG[GA]G
BbsI 1707 GAAGAC GAAGAC(2/6)
BbvCI 1978 CCTCAGC CCTCAGC(-5/-2)
BcnI 213 CCGGG CC^[GC]GG
BpuAI 1707 GAAGAC GAAGAC(2/6)
BsaI 1852 GAGACC GGTCTC(1/5)
BsiHKAI 2002 GAGCTC G[AT]GC[AT]^C
BsmBI 1543 CGTCTC CGTCTC(1/5)
BsmI 693 GAATGC GAATGC(1/-1)
BsoBI 1061 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1841 TGTACA T^GTACA
BspDI 306 ATCGAT AT^CGAT
BspHI 1041 TCATGA T^CATGA
BsrGI 1841 TGTACA T^GTACA
BstXI 2117 CCAGACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 306 ATCGAT AT^CGAT
EcoRI 1786 GAATTC G^AATTC
HaeII 2203 AGCGCC [GA]GCGC^[CT]
HapII 213 CCGG C^CGG
HindIII 1796 AAGCTT A^AGCTT
HpaII 213 CCGG C^CGG
MaeII 1258 ACGT A^CGT
MspI 213 CCGG C^CGG
NciI 213 CCGGG CC^[GC]GG
NheI 2079 GCTAGC G^CTAGC
RcaI 1041 TCATGA T^CATGA
SacI 2002 GAGCTC GAGCT^C
ScaI 1937 AGTACT AGT^ACT
SspBI 1841 TGTACA T^GTACA
SwaI 2028 ATTTAAAT ATTT^AAAT
XmnI 1102 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1489 GAATTT [GA]^AATT[CT]
1786 GAATTC
ApoI 1489 GAATTT [GA]^AATT[CT]
1786 GAATTC
BanII 1862 GAGCCC G[GA]GC[CT]^C
2002 GAGCTC
BmyI 1862 GAGCCC G[AGT]GC[ACT]^C
2002 GAGCTC
BsgI 515 CTGCAC GTGCAG(16/14)
2230 GTGCAG
Bsp1286I 1862 GAGCCC G[AGT]GC[ACT]^C
2002 GAGCTC
BspMI 513 ACCTGC ACCTGC(4/8)
421 GCAGGT
BstF5I 533 CATCC GGATG(2/0)
1065 GGATG
BstNI 705 CCAGG CC^[AT]GG
1111 CCTGG
CfoI 789 GCGC GCG^C
2204 GCGC
EcoRII 705 CCAGG ^CC[AT]GG
1111 CCTGG
FokI 533 CATCC GGATG(9/13)
1065 GGATG
HhaI 789 GCGC GCG^C
2204 GCGC
HinP1I 789 GCGC G^CGC
2204 GCGC
HincII 120 GTTGAC GT[CT]^[GA]AC
1561 GTTGAC
HindII 120 GTTGAC GT[CT]^[GA]AC
1561 GTTGAC
MaeIII 28 GTGAC ^GT[ACGT]AC
667 GTCAC
MslI 1333 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2223 CACTCAGGTG
MvaI 705 CCAGG CC^[AT]GG
1111 CCTGG
NcoI 743 CCATGG C^CATGG
1388 CCATGG
NdeI 2042 CATATG CA^TATG
2195 CATATG
Tsp45I 28 GTGAC ^GT[GC]AC
667 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
AvaII BbvI BclI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsrDI BsrI BssKI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI Eco81I EcoT14I FauI FbaI
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MspA1I MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I