(Circular) (Six-base) MAPSORT of: pFN21AB5766.seq Check: 2029 from: 1 to: 6946 pFN21AB5766 6946 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2417 2527 2417 Size: 110 6836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4756 278 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4289 1937 Size: 2352 4594 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2400 2589 3669 4013 4130 4166 5009 Size: 462 306 189 1080 344 117 36 843 Cuts at: 5009 6393 6661 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1080 843 462 344 306 268 189 117 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 5845 6822 1203 Size: 4642 977 1327 Fragments arranged by size: 4642 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 5675 5675 Size: 6946 AleI CACnn'nnGTG Cuts at: 1166 3342 4232 1166 Size: 2176 890 3880 Fragments arranged by size: 3880 2176 890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2703 1184 Size: 1519 5427 AlwNI CAG_nnn'CTG Cuts at: 2453 3508 3647 3967 3991 4689 6261 2453 Size: 1055 139 320 24 698 1572 3138 Fragments arranged by size: 3138 1572 1055 698 320 139 24 ApaLI G'TGCA_C Cuts at: 5003 6159 5003 Size: 1156 5790 ApoI r'AATT_y Cuts at: 1493 3494 3791 4255 4481 4633 6742 1493 Size: 2001 297 464 226 152 2109 1697 Fragments arranged by size: 2109 2001 1697 464 297 226 152 AseI AT'TA_AT Cuts at: 160 1032 5500 160 Size: 872 4468 1606 Fragments arranged by size: 4468 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 3066 4271 1870 Size: 73 1123 1205 4545 Fragments arranged by size: 4545 1205 1123 73 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4267 5722 618 Size: 325 217 627 2480 1455 1842 Fragments arranged by size: 2480 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3871 4011 4265 1391 Size: 2480 140 254 4072 Fragments arranged by size: 4072 2480 254 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3719 928 Size: 971 1820 4155 Fragments arranged by size: 4155 1820 971 BbvCI CC'TCA_GC Cuts at: 3984 3984 Size: 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5137 5171 1903 Size: 34 50 34 3116 34 3678 Fragments arranged by size: 3678 3116 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4838 6048 4838 Size: 1210 5736 BclI T'GATC_A Cuts at: 1548 2485 3208 3445 1548 Size: 937 723 237 5049 Fragments arranged by size: 5049 937 723 237 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5557 136 Size: 107 122 71 631 605 3885 1525 Fragments arranged by size: 3885 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6941 6941 Size: 6946 BlpI GC'TnA_GC Cuts at: 4363 4363 Size: 6946 Bme1580I G_kGCm'C Cuts at: 1790 5007 6163 1790 Size: 3217 1156 2573 Fragments arranged by size: 3217 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4677 5635 442 Size: 416 53 83 399 24 3260 958 1753 Fragments arranged by size: 3260 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4088 1056 Size: 3032 3914 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3538 3538 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 2394 3087 3807 4100 5606 1239 Size: 927 228 693 720 293 1506 2579 Fragments arranged by size: 2579 1506 927 720 693 293 228 Bpu10I CC'TnA_GC Cuts at: 3027 3984 3027 Size: 957 5989 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5071 5936 6234 5071 Size: 865 298 5783 Fragments arranged by size: 5783 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3851 5609 882 Size: 617 2352 1758 2219 Fragments arranged by size: 2352 2219 1758 617 BsaAI yAC'GTr Cuts at: 493 1204 4737 493 Size: 711 3533 2702 Fragments arranged by size: 3533 2702 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4753 5135 5770 275 Size: 53 83 186 936 3220 382 635 1451 Fragments arranged by size: 3220 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5379 5777 6051 6198 6609 5379 Size: 398 274 147 411 5716 Fragments arranged by size: 5716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3935 3965 1178 Size: 30 165 30 2532 30 4159 Fragments arranged by size: 4159 2532 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6946 BseYI C'CCAG_C Cuts at: 2446 2924 3414 3745 6149 6645 2446 Size: 478 490 331 2404 496 2747 Fragments arranged by size: 2747 2404 496 490 478 331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2505 4256 1163 Size: 573 769 1751 3853 Fragments arranged by size: 3853 1751 769 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 5007 5092 6163 4011 Size: 996 85 1071 4794 Fragments arranged by size: 4794 1071 996 85 BsmI GAATG_Cn' Cuts at: 2704 4457 4550 2704 Size: 1753 93 5100 Fragments arranged by size: 5100 1753 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3554 3554 Size: 6946 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3871 4011 4265 5007 5092 6163 1391 Size: 399 2081 140 254 742 85 1071 2174 Fragments arranged by size: 2174 2081 1071 742 399 254 140 85 BspEI T'CCGG_A Cuts at: 6609 6609 Size: 6946 BspHI T'CATG_A Cuts at: 3046 4835 6565 3046 Size: 1789 1730 3427 Fragments arranged by size: 3427 1789 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2417 2527 4301 844 Size: 1573 110 1774 3489 Fragments arranged by size: 3489 1774 1573 110 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2081 2297 3183 3915 5441 5615 65 Size: 1183 833 216 886 732 1526 174 1396 Fragments arranged by size: 1526 1396 1183 886 833 732 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5590 5777 1700 Size: 110 3780 187 2869 Fragments arranged by size: 3780 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3846 96 Size: 3750 3196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6946 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstXI CCAn_nnnn'nTGG Cuts at: 4129 4129 Size: 6946 BstYI r'GATC_y Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941 Size: 2304 367 385 17 1452 86 12 346 Cuts at: 6941 1972 Size: 1977 Fragments arranged by size: 2304 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2466 3464 4017 2466 Size: 998 553 5395 Fragments arranged by size: 5395 998 553 BtgI C'CryG_G Cuts at: 513 1065 2748 3393 3838 513 Size: 552 1683 645 445 3621 Fragments arranged by size: 3621 1683 645 552 445 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6207 Fragments arranged by size: 6207 683 56 BtsI GCAGTG_nn' Cuts at: 4462 5256 5276 4462 Size: 794 20 6132 Fragments arranged by size: 6132 794 20 ClaI AT'CG_AT Cuts at: 1554 2312 1554 Size: 758 6188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6911 DraI TTT'AAA Cuts at: 2478 3079 4036 4250 2478 Size: 601 957 214 5174 Fragments arranged by size: 5174 957 601 214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5953 809 Size: 507 4637 1802 Fragments arranged by size: 4637 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4393 5282 6911 Size: 54 1425 50 275 18 2563 889 1629 Cuts at: 6911 8 Size: 43 Fragments arranged by size: 2563 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2865 3645 3849 4876 1353 Size: 911 601 780 204 1027 3423 Fragments arranged by size: 3423 1027 911 780 601 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5533 5907 6053 387 Size: 1064 138 3944 374 146 1280 Fragments arranged by size: 3944 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4009 4009 Size: 6946 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2166 2394 2400 2589 3087 3669 Size: 393 462 72 228 6 189 498 582 Cuts at: 3669 3807 4013 4100 4130 4166 5009 5606 6393 Size: 138 206 87 30 36 843 597 787 Cuts at: 6393 6661 1239 Size: 268 1524 Fragments arranged by size: 1524 843 787 597 582 498 462 393 268 228 206 189 138 87 72 36 30 6 EcoRI G'AATT_C Cuts at: 3791 4255 4633 3791 Size: 464 378 6104 Fragments arranged by size: 6104 464 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3878 3878 Size: 6946 FspI TGC'GCA Cuts at: 5452 5452 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 4212 6093 1678 Size: 2534 1881 2531 Fragments arranged by size: 2534 2531 1881 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2765 2797 2965 Size: 32 135 32 851 32 310 32 168 Cuts at: 2965 2997 3143 3161 3175 3193 5587 5619 5661 Size: 32 146 18 14 18 2394 32 42 Cuts at: 5661 5693 1373 Size: 32 2626 Fragments arranged by size: 2626 2394 851 310 168 146 135 42 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2127 3568 4290 4536 669 Size: 988 281 189 1441 722 246 3079 Fragments arranged by size: 3079 1441 988 722 281 246 189 HindIII A'AGCT_T Cuts at: 748 3801 748 Size: 3053 3893 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2754 6060 6244 1305 Size: 1449 3306 184 2007 Fragments arranged by size: 3306 2007 1449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5124 Fragments arranged by size: 5124 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3342 4232 4904 5263 5422 518 Size: 648 2176 890 672 359 159 2042 Fragments arranged by size: 2176 2042 890 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3214 3434 3745 4108 4362 5039 6187 6432 Size: 1608 220 311 363 254 677 1148 245 Cuts at: 6432 1606 Size: 2120 Fragments arranged by size: 2120 1608 1148 677 363 311 254 245 220 NcoI C'CATG_G Cuts at: 513 1065 2748 3393 513 Size: 552 1683 645 4066 Fragments arranged by size: 4066 1683 645 552 NdeI CA'TA_TG Cuts at: 387 4048 4201 387 Size: 3661 153 3132 Fragments arranged by size: 3661 3132 153 NheI G'CTAG_C Cuts at: 1052 4084 1052 Size: 3032 3914 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 4304 5849 1132 Size: 97 3075 1545 2229 Fragments arranged by size: 3075 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6946 PciI A'CATG_T Cuts at: 5845 5845 Size: 6946 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6946 PfoI T'CCnGG_A Cuts at: 2217 2217 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 2289 4516 2289 Size: 2227 4719 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3519 3551 3519 Size: 32 6914 PstI C_TGCA'G Cuts at: 830 2284 2458 2682 2953 3316 3727 4298 830 Size: 1454 174 224 271 363 411 571 3478 Fragments arranged by size: 3478 1454 571 411 363 271 224 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 3214 3434 4108 3214 Size: 220 674 6052 Fragments arranged by size: 6052 674 220 SacI G_AGCT'C Cuts at: 4011 4011 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SbfI CC_TGCA'GG Cuts at: 4298 4298 Size: 6946 ScaI AGT'ACT Cuts at: 3944 5194 6906 3944 Size: 1250 1712 3984 Fragments arranged by size: 3984 1712 1250 SfcI C'TryA_G Cuts at: 826 1045 2280 2454 2678 2949 3312 3723 3959 Size: 219 1235 174 224 271 363 411 236 Cuts at: 3959 4294 5429 6110 6301 826 Size: 335 1135 681 191 1471 Fragments arranged by size: 1471 1235 1135 681 411 363 335 271 236 224 219 191 174 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5050 5951 6213 820 Size: 197 926 3107 901 262 1553 Fragments arranged by size: 3107 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 4304 1132 Size: 3172 3774 SspI AAT'ATT Cuts at: 5 52 4870 5 Size: 47 4818 2081 Fragments arranged by size: 4818 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6946 StyI C'CwwG_G Cuts at: 513 1065 2748 3270 3393 4385 513 Size: 552 1683 522 123 992 3074 Fragments arranged by size: 3074 1683 992 552 522 123 SwaI ATTT'AAAT Cuts at: 4036 4036 Size: 6946 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2431 3108 3812 4973 4990 5143 5328 Size: 1255 851 677 704 1161 17 153 185 Cuts at: 5328 5773 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1161 851 704 677 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3702 3846 3942 Size: 274 80 33 51 1443 1725 144 96 Cuts at: 3942 5192 6904 96 Size: 1250 1712 138 Fragments arranged by size: 1725 1712 1443 1250 274 144 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3723 3755 6906 6938 3723 Size: 32 3151 32 3731 Fragments arranged by size: 3731 3151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 3111 5075 3111 Size: 1964 4982 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4754 276 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI