(Circular) (Six-base) MAPSORT of: pFN21AB5868.seq Check: 5137 from: 1 to: 6184 pFN21AB5868 6184 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2643 3186 3527 1937 Size: 706 543 341 4594 Fragments arranged by size: 4594 706 543 341 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4247 5631 5899 1632 Size: 2615 1384 268 1917 Fragments arranged by size: 2615 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 2511 2996 5083 6060 1203 Size: 1308 485 2087 977 1327 Fragments arranged by size: 2087 1327 1308 977 485 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2620 1184 Size: 1436 4748 AlwNI CAG_nnn'CTG Cuts at: 2387 3927 5499 2387 Size: 1540 1572 3072 Fragments arranged by size: 3072 1572 1540 ApaLI G'TGCA_C Cuts at: 2533 4241 5397 2533 Size: 1708 1156 3320 Fragments arranged by size: 3320 1708 1156 ApoI r'AATT_y Cuts at: 1493 2948 3493 3719 3871 5980 1493 Size: 1455 545 226 152 2109 1697 Fragments arranged by size: 2109 1697 1455 545 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2345 3509 1870 Size: 73 402 1164 4545 Fragments arranged by size: 4545 1164 402 73 BamHI G'GATC_C Cuts at: 2110 2588 3514 3881 2110 Size: 478 926 367 4413 Fragments arranged by size: 4413 926 478 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3505 4960 618 Size: 325 217 627 1718 1455 1842 Fragments arranged by size: 1842 1718 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3503 1391 Size: 2112 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 4223 Fragments arranged by size: 4223 990 971 BbvCI CC'TCA_GC Cuts at: 2256 2442 2256 Size: 186 5998 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2519 2553 3120 3154 4375 4409 1903 Size: 34 582 34 567 34 1221 34 3678 Fragments arranged by size: 3678 1221 582 567 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2198 2483 2966 4076 5286 2198 Size: 285 483 1110 1210 3096 Fragments arranged by size: 3096 1210 1110 483 285 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2799 2858 3601 2799 Size: 59 743 5382 Fragments arranged by size: 5382 743 59 Bme1580I G_kGCm'C Cuts at: 1790 2537 2609 2966 4245 5401 1790 Size: 747 72 357 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 747 357 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3137 3169 3915 Size: 416 53 83 399 24 1720 32 746 Cuts at: 3915 4873 442 Size: 958 1753 Fragments arranged by size: 1753 1720 958 746 416 399 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2225 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3419 4844 1239 Size: 2180 1425 2579 Fragments arranged by size: 2579 2180 1425 Bpu10I CC'TnA_GC Cuts at: 2256 2442 2256 Size: 186 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2890 4309 5174 5472 2890 Size: 1419 865 298 3602 Fragments arranged by size: 3602 1419 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3014 4847 882 Size: 617 1515 1833 2219 Fragments arranged by size: 2219 1833 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 2079 3975 493 Size: 711 875 1896 2702 Fragments arranged by size: 2702 1896 875 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2650 2680 1178 Size: 30 165 30 1247 30 4682 Fragments arranged by size: 4682 1247 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6184 BseYI C'CCAG_C Cuts at: 2431 5387 5883 2431 Size: 2956 496 2732 Fragments arranged by size: 2956 2732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2502 2740 1163 Size: 573 766 238 4607 Fragments arranged by size: 4607 766 573 238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2537 2800 3357 4245 4330 5401 2537 Size: 263 557 888 85 1071 3320 Fragments arranged by size: 3320 1071 888 557 263 85 BsmI GAATG_Cn' Cuts at: 2448 2900 3038 3695 3788 2448 Size: 452 138 657 93 4844 Fragments arranged by size: 4844 657 452 138 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 2609 2800 2966 3357 3503 4245 Size: 399 747 72 191 166 391 146 742 Cuts at: 4245 4330 5401 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 391 191 166 146 85 72 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 4073 5803 4073 Size: 1730 4454 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2765 3539 844 Size: 1921 774 3489 Fragments arranged by size: 3489 1921 774 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2093 2132 3351 4679 4853 65 Size: 1183 845 39 1219 1328 174 1396 Fragments arranged by size: 1396 1328 1219 1183 845 174 39 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 3356 4244 5256 3356 Size: 888 1012 4284 Fragments arranged by size: 4284 1012 888 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2323 2323 Size: 6184 BstYI r'GATC_y Cuts at: 1972 2110 2588 3103 3514 3881 4266 4283 5735 Size: 138 478 515 411 367 385 17 1452 Cuts at: 5735 5821 5833 6179 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 515 478 411 385 367 346 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2287 2287 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5632 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 2535 2642 2810 2869 3700 4494 4514 2535 Size: 107 168 59 831 794 20 4205 Fragments arranged by size: 4205 831 794 168 107 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2287 2322 3447 3482 529 Size: 35 1723 35 1125 35 3231 Fragments arranged by size: 3231 1723 1125 35 35 35 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3330 5191 809 Size: 507 2014 1861 1802 Fragments arranged by size: 2014 1861 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2401 3631 Size: 54 1425 50 275 18 531 40 1230 Cuts at: 3631 4520 6149 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1230 889 531 275 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4114 1353 Size: 2761 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2440 4771 5145 5291 387 Size: 1064 138 851 2331 374 146 1280 Fragments arranged by size: 2331 1280 1064 851 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3419 4247 4844 5631 5899 1239 Size: 393 1787 828 597 787 268 1524 Fragments arranged by size: 1787 1524 828 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2384 3426 2384 Size: 1042 5142 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 EcoRV GAT'ATC Cuts at: 2618 2618 Size: 6184 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2045 5331 1678 Size: 367 3286 2531 Fragments arranged by size: 3286 2531 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3372 3404 4825 4857 4899 Size: 32 135 32 1800 32 1421 32 42 Cuts at: 4899 4931 1373 Size: 32 2626 Fragments arranged by size: 2626 1800 1421 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2191 3528 3774 669 Size: 988 281 253 1337 246 3079 Fragments arranged by size: 3079 1337 988 281 253 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 2735 3783 2735 Size: 1048 5136 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 3035 5298 5482 1305 Size: 1176 554 2263 184 2007 Fragments arranged by size: 2263 2007 1176 554 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3831 Fragments arranged by size: 3831 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2602 2959 4142 4501 4660 518 Size: 648 1436 357 1183 359 159 2042 Fragments arranged by size: 2042 1436 1183 648 359 357 159 MspA1I CmG'CkG Cuts at: 1606 2435 3600 4277 5425 5670 1606 Size: 829 1165 677 1148 245 2120 Fragments arranged by size: 2120 1165 1148 829 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 2601 3000 3542 5087 1132 Size: 97 1372 399 542 1545 2229 Fragments arranged by size: 2229 1545 1372 542 399 97 PasI CC'CwG_GG Cuts at: 1348 2968 1348 Size: 1620 4564 PciI A'CATG_T Cuts at: 2996 5083 2996 Size: 2087 4097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PfoI T'CCnGG_A Cuts at: 2611 2611 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3426 3426 Size: 6184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 2377 2542 3536 830 Size: 1547 165 994 3478 Fragments arranged by size: 3478 1547 994 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2435 2435 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2373 2538 2554 3532 4667 5348 5539 Size: 219 1328 165 16 978 1135 681 191 Cuts at: 5539 826 Size: 1471 Fragments arranged by size: 1471 1328 1135 978 681 219 191 165 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2347 3511 1872 Size: 475 1164 4545 Fragments arranged by size: 4545 1164 475 SmlI C'TyrA_G Cuts at: 820 1017 1943 2905 4288 5189 5451 820 Size: 197 926 962 1383 901 262 1553 Fragments arranged by size: 1553 1383 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6184 StyI C'CwwG_G Cuts at: 513 1065 3623 513 Size: 552 2558 3074 Fragments arranged by size: 3074 2558 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2964 2996 3035 3067 6144 6176 2964 Size: 32 39 32 3077 32 2972 Fragments arranged by size: 3077 2972 39 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2153 1316 Size: 837 5347 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2723 2723 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2345 3509 1870 Size: 475 1164 4545 Fragments arranged by size: 4545 1164 475 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI