Restriction map for
pFN21AB5861
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflIII Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AvrII BalI
BbrPI BbsI BcgI BciVI BclI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BspEI BspLU11I
BspMI BsrDI BssHII Bst1107I BstBI
BstZ17I CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco52I EcoRI
EcoT22I FbaI FseI HindIII HpaI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tsp509I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 158 GGTACC G^GTACC
AflII 544 CTTAAG C^TTAAG
AgeI 155 ACCGGT A^CCGGT
AhdI 123 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 530 AGCGCT AGC^GCT
ApaI 13 GGGCCC GGGCC^C
Asp718I 158 GGTACC G^GTACC
AspEI 123 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 268 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 881 TGCGCA TGC^GCA
BamHI 369 GGATCC G^GATCC
BbeI 96 GGCGCC GGCGC^C
BfaI 1123 CTAG C^TAG
BfrI 544 CTTAAG C^TTAAG
BlpI 252 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 252 GCTGAGC GC^T[ACGT]AGC
BsaI 112 GAGACC GGTCTC(1/5)
BsaWI 155 ACCGGT [AT]^CCGG[AT]
BsgI 1180 CTGCAC GTGCAG(16/14)
BsmI 1083 GAATGC GAATGC(1/-1)
Bsp1407I 378 TGTACA T^GTACA
BspDI 175 ATCGAT AT^CGAT
BspHI 516 TCATGA T^CATGA
BsrGI 378 TGTACA T^GTACA
BstAPI 1170 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1306 GGTGACC G^GT[ACGT]ACC
BstPI 1306 GGTGACC G^GT[ACGT]ACC
BstXI 809 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 369 GGATCC [GA]^GATC[CT]
CelII 252 GCTGAGC GC^T[ACGT]AGC
ClaI 175 ATCGAT AT^CGAT
EaeI 479 CGGCCA [CT]^GGCC[GA]
Eam1105I 123 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 530 AGCGCT AGC^GCT
EcoO65I 1306 GGTGACC G^GT[ACGT]ACC
EcoRV 757 GATATC GAT^ATC
FspI 881 TGCGCA TGC^GCA
HincII 655 GTCAAC GT[CT]^[GA]AC
HindII 655 GTCAAC GT[CT]^[GA]AC
KasI 96 GGCGCC G^GCGCC
KpnI 158 GGTACC GGTAC^C
MaeI 1123 CTAG C^TAG
MflI 369 GGATCC [GA]^GATC[CT]
NaeI 632 GCCGGC GCC^GGC
NarI 96 GGCGCC GG^CGCC
NgoMIV 632 GCCGGC G^CCGGC
PaeR7I 1345 CTCGAG C^TCGAG
PflFI 268 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 155 ACCGGT A^CCGGT
PpuMI 488 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 516 TCATGA T^CATGA
SacI 304 GAGCTC GAGCT^C
SfoI 96 GGCGCC GGC^GCC
SphI 884 GCATGC GCATG^C
SspBI 378 TGTACA T^GTACA
StuI 141 AGGCCT AGG^CCT
TfiI 396 GAATC G^A[AT]TC
Tth111I 268 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 374 CCAATGTACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1345 CTCGAG C^TCGAG
XhoII 369 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 96 GGCGCC G[GA]^CG[CT]C
358 GACGCC
AlwNI 787 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1375 CAGCATCTG
AocI 1246 CCTGAGG CC^T[ACGT]AGG
1357 CCTGAGG
AspHI 304 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
BanII 13 GGGCCC G[GA]GC[CT]^C
304 GAGCTC
BbvCI 1101 CCTCAGC CCTCAGC(-5/-2)
1038 GCTGAGG
BglI 67 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1066 GCCCGGGAGGC
BsaHI 96 GGCGCC G[GA]^CG[CT]C
358 GACGCC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
BsmBI 187 GAGACG CGTCTC(1/5)
558 GAGACG
BsrBI 243 GAGCGG CCGCTC(-3/-3)
1348 GAGCGG
BssSI 553 CACGAG CACGAG(-5/-1)
931 CTCGTG
Bsu36I 1246 CCTGAGG CC^T[ACGT]AGG
1357 CCTGAGG
Eco81I 1246 CCTGAGG CC^T[ACGT]AGG
1357 CCTGAGG
EcoNI 162 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1101 CCTCAGCCAGG
EcoT14I 809 CCAAGG C^C[AT][AT]GG
1088 CCAAGG
HgaI 358 GACGC GACGC(5/10)
505 GACGC
Hin1I 96 GGCGCC G[GA]^CG[CT]C
358 GACGCC
HphI 1306 GGTGA GGTGA(8/7)
806 TCACC
MaeII 284 ACGT A^CGT
659 ACGT
MseI 545 TTAA T^TAA
728 TTAA
MspA1I 40 CCGCTG C[AC]G^C[GT]G
1192 CAGCGG
PleI 129 GAGTC GAGTC(4/5)
574 GAGTC
PstI 214 CTGCAG CTGCA^G
1165 CTGCAG
SapI 619 GAAGAGC GCTCTTC(1/4)
1033 GAAGAGC
SfcI 214 CTGCAG C^T[GA][CT]AG
1165 CTGCAG
SmaI 485 CCCGGG CCC^GGG
1067 CCCGGG
StyI 809 CCAAGG C^C[AT][AT]GG
1088 CCAAGG
Tru9I 545 TTAA T^TAA
728 TTAA
XmaCI 485 CCCGGG C^CCGGG
1067 CCCGGG
XmaI 485 CCCGGG C^CCGGG
1067 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp45I
TspRI TthHB8I