(Circular) (Six-base) MAPSORT of: pFN21AB5861.seq Check: 1222 from: 1 to: 6088 pFN21AB5861 6088 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 2163 3409 2163 Size: 1246 4842 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3318 4151 5535 5803 1632 Size: 1686 833 1384 268 1917 Fragments arranged by size: 1917 1686 1384 833 268 AfeI AGC'GCT Cuts at: 2537 2537 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 2549 820 Size: 197 1532 4359 Fragments arranged by size: 4359 1532 197 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 2160 4919 2160 Size: 2759 3329 AhdI GACnn_n'nnGTC Cuts at: 2133 4817 2133 Size: 2684 3404 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2797 3385 3831 5403 2797 Size: 588 446 1572 3482 Fragments arranged by size: 3482 1572 588 446 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 6088 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2490 3072 3350 3413 1870 Size: 73 79 468 582 278 63 4545 Fragments arranged by size: 4545 582 468 278 79 73 63 BamHI G'GATC_C Cuts at: 2374 3418 3785 2374 Size: 1044 367 4677 Fragments arranged by size: 4677 1044 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2163 2640 3373 3409 Size: 325 217 627 314 62 477 733 36 Cuts at: 3409 4864 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 733 627 477 325 314 217 62 36 BanII G_rGCy'C Cuts at: 1391 2022 2313 3407 1391 Size: 631 291 1094 4072 Fragments arranged by size: 4072 1094 631 291 BbeI G_GCGC'C Cuts at: 2105 2105 Size: 6088 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5117 BbvCI CC'TCA_GC Cuts at: 3044 3107 3044 Size: 63 6025 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4279 4313 1903 Size: 34 50 34 2258 34 3678 Fragments arranged by size: 3678 2258 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 3077 4699 Size: 107 122 71 631 605 406 999 1622 Cuts at: 4699 136 Size: 1525 Fragments arranged by size: 1622 1525 999 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 2258 3505 2258 Size: 1247 4841 Bme1580I G_kGCm'C Cuts at: 1790 2022 3378 4149 5305 1790 Size: 232 1356 771 1156 2573 Fragments arranged by size: 2573 1356 1156 771 232 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 5116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 2594 2677 Size: 416 53 83 399 24 1080 97 83 Cuts at: 2677 3819 4777 442 Size: 1142 958 1753 Fragments arranged by size: 1753 1142 1080 958 416 399 97 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2048 2048 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2153 2210 2486 2597 2819 2843 2966 Size: 872 42 57 276 111 222 24 123 Cuts at: 2966 3170 3288 4748 1239 Size: 204 118 1460 2579 Fragments arranged by size: 2579 1460 872 276 222 204 123 118 111 57 42 24 Bpu10I CC'TnA_GC Cuts at: 3044 3107 3044 Size: 63 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 2333 3248 4213 5078 5376 2297 Size: 36 915 965 865 298 3009 Fragments arranged by size: 3009 965 915 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 4751 882 Size: 617 612 2640 2219 Fragments arranged by size: 2640 2219 617 612 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 2364 3895 4277 Size: 53 83 186 936 569 262 1531 382 Cuts at: 4277 4912 275 Size: 635 1451 Fragments arranged by size: 1531 1451 936 635 569 382 262 186 83 53 BsaWI w'CCGG_w Cuts at: 2160 4521 4919 5193 5340 5751 2160 Size: 2361 398 274 147 411 2497 Fragments arranged by size: 2497 2361 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2816 2846 1178 Size: 30 165 30 1413 30 4420 Fragments arranged by size: 4420 1413 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2606 2717 2864 2888 2891 2963 3074 Size: 943 477 111 147 24 3 72 111 Cuts at: 3074 1186 Size: 4200 Fragments arranged by size: 4200 943 477 147 111 111 72 24 3 BseYI C'CCAG_C Cuts at: 3000 3099 3298 3378 5291 5787 3000 Size: 99 199 80 1913 496 3301 Fragments arranged by size: 3301 1913 496 199 99 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3170 1163 Size: 573 1434 4081 Fragments arranged by size: 4081 1434 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 2652 4149 4234 5305 2313 Size: 339 1497 85 1071 3096 Fragments arranged by size: 3096 1497 1071 339 85 BsmI GAATG_Cn' Cuts at: 3094 3599 3692 3094 Size: 505 93 5490 Fragments arranged by size: 5490 505 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2557 2186 Size: 371 5717 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2313 2652 3378 3407 4149 4234 Size: 399 232 291 339 726 29 742 85 Cuts at: 4234 5305 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 726 399 339 291 232 85 29 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 2521 3977 5707 2521 Size: 1456 1730 2902 Fragments arranged by size: 2902 1730 1456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2250 3355 3975 1394 Size: 429 427 1105 620 3507 Fragments arranged by size: 3507 1105 620 429 427 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 2637 3308 4732 4919 1700 Size: 110 350 477 671 1424 187 2869 Fragments arranged by size: 2869 1424 671 477 350 187 110 BsrGI T'GTAC_A Cuts at: 96 2383 96 Size: 2287 3801 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2558 2936 4148 5160 2558 Size: 378 1212 1012 3486 Fragments arranged by size: 3486 1212 1012 378 BstAPI GCAn_nnn'nTGC Cuts at: 794 3181 794 Size: 2387 3701 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 3311 1184 Size: 2127 3961 BstXI CCAn_nnnn'nTGG Cuts at: 2821 2821 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2374 3418 3785 4170 4187 5639 5725 5737 Size: 402 1044 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1044 402 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3252 3363 3252 Size: 111 5977 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 3604 4398 4418 3604 Size: 794 20 5274 Fragments arranged by size: 5274 794 20 ClaI AT'CG_AT Cuts at: 1554 2181 1554 Size: 627 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 3535 4424 Size: 54 1425 50 275 18 654 1051 889 Cuts at: 4424 6053 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1051 889 654 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 2619 2813 3033 3051 3155 4018 1353 Size: 1167 99 194 220 18 104 863 3423 Fragments arranged by size: 3423 1167 863 220 194 104 99 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2624 4675 5049 5195 387 Size: 1064 138 1035 2051 374 146 1280 Fragments arranged by size: 2051 1280 1064 1035 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2311 Size: 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2153 2210 2486 2597 2819 2843 Size: 393 479 42 57 276 111 222 24 Cuts at: 2843 2966 3170 3288 3318 4151 4748 5535 5803 Size: 123 204 118 30 833 597 787 268 Cuts at: 5803 1239 Size: 1524 Fragments arranged by size: 1524 833 787 597 479 393 276 268 222 204 123 118 111 57 42 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2171 3110 2171 Size: 939 5149 EcoO109I rG'GnC_Cy Cuts at: 2019 2494 3235 2019 Size: 475 741 4872 Fragments arranged by size: 4872 741 475 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 EcoRV GAT'ATC Cuts at: 2764 2764 Size: 6088 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 6088 FspI TGC'GCA Cuts at: 2888 4594 2888 Size: 1706 4382 HaeII r_GCGC'y Cuts at: 1678 2105 2539 2638 3052 3106 5235 1678 Size: 427 434 99 414 54 2129 2531 Fragments arranged by size: 2531 2129 434 427 414 99 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 3109 3141 4729 Size: 32 135 32 547 32 958 32 1588 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1588 958 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3432 3678 669 Size: 988 281 724 770 246 3079 Fragments arranged by size: 3079 988 770 724 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KasI G'GCGC_C Cuts at: 2101 2101 Size: 6088 KpnI G_GTAC'C Cuts at: 2167 3413 2167 Size: 1246 4842 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 3199 3504 4181 5329 5574 1606 Size: 441 1152 305 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 677 441 305 245 NaeI GCC'GGC Cuts at: 2639 2639 Size: 6088 NarI GG'CG_CC Cuts at: 2102 2102 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2637 2637 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2863 2893 2903 3446 4991 1132 Size: 97 1634 30 10 543 1545 2229 Fragments arranged by size: 2229 1634 1545 543 97 30 10 PasI CC'CwG_GG Cuts at: 1348 3139 1348 Size: 1791 4297 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 3385 1108 Size: 2277 3811 PfoI T'CCnGG_A Cuts at: 2169 2781 2169 Size: 612 5476 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2494 2494 Size: 6088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 6088 PstI C_TGCA'G Cuts at: 830 2223 3174 3440 830 Size: 1393 951 266 3478 Fragments arranged by size: 3478 1393 951 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SacI G_AGCT'C Cuts at: 2313 2313 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 2619 3033 2619 Size: 414 5674 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2219 3170 3436 4571 5252 5443 826 Size: 219 1174 951 266 1135 681 191 1471 Fragments arranged by size: 1471 1174 1135 951 681 266 219 191 SfoI GGC'GCC Cuts at: 2103 2103 Size: 6088 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 2492 3074 3415 1872 Size: 620 582 341 4545 Fragments arranged by size: 4545 620 582 341 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2348 2549 3263 3350 4192 Size: 197 926 369 36 201 714 87 842 Cuts at: 4192 5093 5355 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 842 714 369 262 201 197 87 36 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 2893 3446 1132 Size: 1761 553 3774 Fragments arranged by size: 3774 1761 553 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 2148 1517 Size: 631 5457 StyI C'CwwG_G Cuts at: 513 1065 2814 3093 3527 513 Size: 552 1749 279 434 3074 Fragments arranged by size: 3074 1749 552 434 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3288 4115 4132 4285 4470 4915 325 Size: 1255 1708 827 17 153 185 445 1498 Fragments arranged by size: 1708 1498 1255 827 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 2185 2383 Size: 274 80 33 51 1443 174 34 198 Cuts at: 2383 4334 6046 96 Size: 1951 1712 138 Fragments arranged by size: 1951 1712 1443 274 198 174 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 2276 1316 Size: 960 5128 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2386 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 3350 1943 Size: 1407 4681 XmaI C'CCGG_G Cuts at: 1870 2490 3072 3413 1870 Size: 620 582 341 4545 Fragments arranged by size: 4545 620 582 341 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI