Restriction map for
pFN21AB5861
(Linear) (Six-base) MAPSORT of: KIBB5861.seq Check: 872 from: 1 to: 1383
KIBB5861.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
Acc65I G'GTAC_C
Cuts at: 0 158 1383
Size: 158 1225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1313 1383
Size: 1313 70
AfeI AGC'GCT
Cuts at: 0 532 1383
Size: 532 851
AflII C'TTAA_G
Cuts at: 0 544 1383
Size: 544 839
AgeI A'CCGG_T
Cuts at: 0 155 1383
Size: 155 1228
AhdI GACnn_n'nnGTC
Cuts at: 0 128 1383
Size: 128 1255
AlwNI CAG_nnn'CTG
Cuts at: 0 792 1380 1383
Size: 792 588 3
Fragments arranged by size:
792 588 3
ApaI G_GGCC'C
Cuts at: 0 17 1383
Size: 17 1366
AvaI C'yCGr_G
Cuts at: 0 17 485 1067 1345 1383
Size: 17 468 582 278 38
Fragments arranged by size:
582 468 278 38 17
BamHI G'GATC_C
Cuts at: 0 369 1383
Size: 369 1014
BanI G'GyrC_C
Cuts at: 0 96 158 635 1368 1383
Size: 96 62 477 733 15
Fragments arranged by size:
733 477 96 62 15
BanII G_rGCy'C
Cuts at: 0 17 308 1383
Size: 17 291 1075
Fragments arranged by size:
1075 291 17
BbeI G_GCGC'C
Cuts at: 0 100 1383
Size: 100 1283
BbvCI CC'TCA_GC
Cuts at: 0 1039 1102 1383
Size: 1039 63 281
Fragments arranged by size:
1039 281 63
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1072 1383
Size: 73 999 311
Fragments arranged by size:
999 311 73
BlpI GC'TnA_GC
Cuts at: 0 253 1383
Size: 253 1130
Bme1580I G_kGCm'C
Cuts at: 0 17 1373 1383
Size: 17 1356 10
Fragments arranged by size:
1356 17 10
BmgBI CAC'GTC
Cuts at: 0 285 1383
Size: 285 1098
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 589 672 1383
Size: 492 97 83 711
Fragments arranged by size:
711 492 97 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 43 1383
Size: 43 1340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 148 205 481 592 814 838 961
Size: 106 42 57 276 111 222 24 123
Cuts at: 961 1165 1283 1383
Size: 204 118 100
Fragments arranged by size:
276 222 204 123 118 111 106 100
57 42 24
Bpu10I CC'TnA_GC
Cuts at: 0 1039 1102 1383
Size: 1039 63 281
Fragments arranged by size:
1039 281 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 328 1243 1383
Size: 292 36 915 140
Fragments arranged by size:
915 292 140 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 1383
Size: 106 1277
BsaHI Gr'CG_yC
Cuts at: 0 97 359 1383
Size: 97 262 1024
Fragments arranged by size:
1024 262 97
BsaWI w'CCGG_w
Cuts at: 0 155 1383
Size: 155 1228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 811 841 1383
Size: 811 30 542
Fragments arranged by size:
811 542 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 601 712 859 883 886 958 1069
Size: 124 477 111 147 24 3 72 111
Cuts at: 1069 1383
Size: 314
Fragments arranged by size:
477 314 147 124 111 111 72 24
3
BseYI C'CCAG_C
Cuts at: 0 995 1094 1293 1373 1383
Size: 995 99 199 80 10
Fragments arranged by size:
995 199 99 80 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1383
Size: 1165 218
BsiHKAI G_wGCw'C
Cuts at: 0 308 647 1383
Size: 308 339 736
Fragments arranged by size:
736 339 308
BsmI GAATG_Cn'
Cuts at: 0 1089 1383
Size: 1089 294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 552 1383
Size: 181 371 831
Fragments arranged by size:
831 371 181
Bsp1286I G_dGCh'C
Cuts at: 0 17 308 647 1373 1383
Size: 17 291 339 726 10
Fragments arranged by size:
726 339 291 17 10
BspHI T'CATG_A
Cuts at: 0 516 1383
Size: 516 867
BsrBI CCG'CTC
Cuts at: 0 245 1350 1383
Size: 245 1105 33
Fragments arranged by size:
1105 245 33
BsrFI r'CCGG_y
Cuts at: 0 155 632 1303 1383
Size: 155 477 671 80
Fragments arranged by size:
671 477 155 80
BsrGI T'GTAC_A
Cuts at: 0 378 1383
Size: 378 1005
BssSI C'ACGA_G
Cuts at: 0 553 931 1383
Size: 553 378 452
Fragments arranged by size:
553 452 378
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1176 1383
Size: 1176 207
BstEII G'GTnAC_C
Cuts at: 0 1306 1383
Size: 1306 77
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 1383
Size: 816 567
BstYI r'GATC_y
Cuts at: 0 369 1383
Size: 369 1014
Bsu36I CC'TnA_GG
Cuts at: 0 1247 1358 1383
Size: 1247 111 25
Fragments arranged by size:
1247 111 25
ClaI AT'CG_AT
Cuts at: 0 176 1383
Size: 176 1207
EaeI y'GGCC_r
Cuts at: 0 479 1383
Size: 479 904
EarI CTCTTCn'nnn_
Cuts at: 0 515 614 808 1028 1046 1150 1383
Size: 515 99 194 220 18 104 233
Fragments arranged by size:
515 233 220 194 104 99 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 619 1383
Size: 619 764
EcoICRI GAG'CTC
Cuts at: 0 306 1383
Size: 306 1077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 148 205 481 592 814 838 961
Size: 106 42 57 276 111 222 24 123
Cuts at: 961 1165 1283 1313 1383
Size: 204 118 30 70
Fragments arranged by size:
276 222 204 123 118 111 106 70
57 42 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 1105 1383
Size: 166 939 278
Fragments arranged by size:
939 278 166
EcoO109I rG'GnC_Cy
Cuts at: 0 14 489 1230 1383
Size: 14 475 741 153
Fragments arranged by size:
741 475 153 14
EcoRV GAT'ATC
Cuts at: 0 759 1383
Size: 759 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 1383
Size: 759 624
FspI TGC'GCA
Cuts at: 0 883 1383
Size: 883 500
HaeII r_GCGC'y
Cuts at: 0 100 534 633 1047 1101 1383
Size: 100 434 99 414 54 282
Fragments arranged by size:
434 414 282 100 99 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 1104 1136 1383
Size: 114 32 958 32 247
Fragments arranged by size:
958 247 114 32 32
HincII GTy'rAC
Cuts at: 0 657 1383
Size: 657 726
KasI G'GCGC_C
Cuts at: 0 96 1383
Size: 96 1287
KpnI G_GTAC'C
Cuts at: 0 162 1383
Size: 162 1221
MspA1I CmG'CkG
Cuts at: 0 42 1194 1383
Size: 42 1152 189
Fragments arranged by size:
1152 189 42
NaeI GCC'GGC
Cuts at: 0 634 1383
Size: 634 749
NarI GG'CG_CC
Cuts at: 0 97 1383
Size: 97 1286
NgoMIV G'CCGG_C
Cuts at: 0 632 1383
Size: 632 751
NspI r_CATG'y
Cuts at: 0 858 888 898 1383
Size: 858 30 10 485
Fragments arranged by size:
858 485 30 10
PasI CC'CwG_GG
Cuts at: 0 1134 1383
Size: 1134 249
PfoI T'CCnGG_A
Cuts at: 0 164 776 1383
Size: 164 612 607
Fragments arranged by size:
612 607 164
PpuMI rG'GwC_Cy
Cuts at: 0 489 1383
Size: 489 894
PspOMI G'GGCC_C
Cuts at: 0 13 1383
Size: 13 1370
PstI C_TGCA'G
Cuts at: 0 218 1169 1383
Size: 218 951 214
Fragments arranged by size:
951 218 214
SacI G_AGCT'C
Cuts at: 0 308 1383
Size: 308 1075
SapI GCTCTTCn'nnn_
Cuts at: 0 614 1028 1383
Size: 614 414 355
Fragments arranged by size:
614 414 355
SfcI C'TryA_G
Cuts at: 0 214 1165 1383
Size: 214 951 218
Fragments arranged by size:
951 218 214
SfoI GGC'GCC
Cuts at: 0 98 1383
Size: 98 1285
SmaI CCC'GGG
Cuts at: 0 487 1069 1383
Size: 487 582 314
Fragments arranged by size:
582 487 314
SmlI C'TyrA_G
Cuts at: 0 307 343 544 1258 1345 1383
Size: 307 36 201 714 87 38
Fragments arranged by size:
714 307 201 87 38 36
SphI G_CATG'C
Cuts at: 0 888 1383
Size: 888 495
StuI AGG'CCT
Cuts at: 0 143 1383
Size: 143 1240
StyI C'CwwG_G
Cuts at: 0 809 1088 1383
Size: 809 279 295
Fragments arranged by size:
809 295 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1283 1383
Size: 1283 100
TatI w'GTAC_w
Cuts at: 0 146 180 378 1383
Size: 146 34 198 1005
Fragments arranged by size:
1005 198 146 34
Tth111I GACn'n_nGTC
Cuts at: 0 271 1383
Size: 271 1112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 1383
Size: 381 1002
XhoI C'TCGA_G
Cuts at: 0 1345 1383
Size: 1345 38
XmaI C'CCGG_G
Cuts at: 0 485 1067 1383
Size: 485 582 316
Fragments arranged by size:
582 485 316
Enzymes that do cut:
Acc65I AcuI AfeI AflII AgeI AhdI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I ClaI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MspA1I NaeI NarI NgoMIV
NspI PasI PfoI PpuMI PspOMI PstI SacI SapI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflIII AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BglII BmtI BsaAI BsaBI
BsiEI BsiWI BspEI BspMI BsrDI BssHII BstBI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI
EcoRI FseI FspAI HindIII HpaI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TstI XbaI XmnI ZraI