Restriction map for
pFN21AB5737
(Circular) (Six-base) MAPSORT of: pFN21AB5737.seq Check: 2704 from: 1 to: 7090
pFN21AB5737 7090 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4900 278
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2586 3219 4433 1937
Size: 649 633 1214 4594
Fragments arranged by size:
4594 1214 649 633
Acc65I G'GTAC_C
Cuts at: 3335 4411 3335
Size: 1076 6014
AclI AA'CG_TT
Cuts at: 1563 5217 5590 1563
Size: 3654 373 3063
Fragments arranged by size:
3654 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2712 3008 3503 3512 4060 5153 6537
Size: 756 324 296 495 9 548 1093 1384
Cuts at: 6537 6805 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1093 756 548 495 324 296
268 9
AfeI AGC'GCT
Cuts at: 3254 3254
Size: 7090
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6893
AflIII A'CryG_T
Cuts at: 1203 5989 6966 1203
Size: 4786 977 1327
Fragments arranged by size:
4786 1327 977
AgeI A'CCGG_T
Cuts at: 5921 5921
Size: 7090
AhdI GACnn_n'nnGTC
Cuts at: 5819 5819
Size: 7090
AleI CACnn'nnGTG
Cuts at: 1166 2894 3620 1166
Size: 1728 726 4636
Fragments arranged by size:
4636 1728 726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7090
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2991 3023 2991
Size: 32 7058
AlwNI CAG_nnn'CTG
Cuts at: 2277 3313 3481 3529 3671 4186 4833 6405 2277
Size: 1036 168 48 142 515 647 1572 2962
Fragments arranged by size:
2962 1572 1036 647 515 168 142 48
ApaI G_GGCC'C
Cuts at: 2078 3208 4023 2078
Size: 1130 815 5145
Fragments arranged by size:
5145 1130 815
ApaLI G'TGCA_C
Cuts at: 3623 5147 6303 3623
Size: 1524 1156 4410
Fragments arranged by size:
4410 1524 1156
ApoI r'AATT_y
Cuts at: 1493 4399 4625 4777 6886 1493
Size: 2906 226 152 2109 1697
Fragments arranged by size:
2906 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5644 160
Size: 872 4612 1606
Fragments arranged by size:
4612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2114 3195 3201 4415 1870
Size: 73 110 61 1081 6 1214 4545
Fragments arranged by size:
4545 1214 1081 110 73 61 6
BamHI G'GATC_C
Cuts at: 2724 4420 4787 2724
Size: 1696 367 5027
Fragments arranged by size:
5027 1696 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2362 2560 3017 3106 3335
Size: 325 217 627 575 198 457 89 229
Cuts at: 3335 4006 4068 4411 5866 618
Size: 671 62 343 1455 1842
Fragments arranged by size:
1842 1455 671 627 575 457 343 325
229 217 198 89 62
BanII G_rGCy'C
Cuts at: 1391 2078 2182 2996 3202 3208 4023 4409 1391
Size: 687 104 814 206 6 815 386 4072
Fragments arranged by size:
4072 815 814 687 386 206 104 6
BbeI G_GCGC'C
Cuts at: 2366 3021 4072 2366
Size: 655 1051 5384
Fragments arranged by size:
5384 1051 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3074 3421 928
Size: 971 1175 347 4597
Fragments arranged by size:
4597 1175 971 347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5281 5315 1903
Size: 34 3344 34 3678
Fragments arranged by size:
3678 3344 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4982 6192 4982
Size: 1210 5880
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7090
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3057 3098 4056
Size: 107 122 71 631 605 1385 41 958
Cuts at: 4056 4261 4381 5701 136
Size: 205 120 1320 1525
Fragments arranged by size:
1525 1385 1320 958 631 605 205 122
120 107 71 41
BglII A'GATC_T
Cuts at: 7085 7085
Size: 7090
BlpI GC'TnA_GC
Cuts at: 4507 4507
Size: 7090
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2078 3111 3208 3627 3946 4023 5151
Size: 245 43 1033 97 419 319 77 1128
Cuts at: 5151 6307 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1128 1033 419 319 245 97
77 43
BmgBI CAC'GTC
Cuts at: 1318 2575 2935 3427 1318
Size: 1257 360 492 4981
Fragments arranged by size:
4981 1257 492 360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2723 3496 4821
Size: 416 53 83 399 24 1306 773 1325
Cuts at: 4821 5779 442
Size: 958 1753
Fragments arranged by size:
1753 1325 1306 958 773 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2142 2630 1056
Size: 1086 488 5516
Fragments arranged by size:
5516 1086 488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2190 2619 2993 3779 5750 1239
Size: 871 80 429 374 786 1971 2579
Fragments arranged by size:
2579 1971 871 786 429 374 80
Bpu10I CC'TnA_GC
Cuts at: 3102 3102
Size: 7090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 5215 6080 6378 2299
Size: 2916 865 298 3011
Fragments arranged by size:
3011 2916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3790 3834 4328 4337 5753 882
Size: 617 2291 44 494 9 1416 2219
Fragments arranged by size:
2291 2219 1416 617 494 44 9
BsaAI yAC'GTr
Cuts at: 493 1204 4881 493
Size: 711 3677 2702
Fragments arranged by size:
3677 2702 711
BsaBI GATnn'nnATC
Cuts at: 7084 7084
Size: 7090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2363 2392 3018 3362
Size: 53 83 186 936 830 29 626 344
Cuts at: 3362 4069 4299 4897 5279 5914 275
Size: 707 230 598 382 635 1451
Fragments arranged by size:
1451 936 830 707 635 626 598 382
344 230 186 83 53 29
BsaWI w'CCGG_w
Cuts at: 5523 5921 6195 6342 6753 5523
Size: 398 274 147 411 5860
Fragments arranged by size:
5860 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2380 2410 2592 2622 2991
Size: 30 165 30 977 30 182 30 369
Cuts at: 2991 3021 3854 3884 1178
Size: 30 833 30 4384
Fragments arranged by size:
4384 977 833 369 182 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2401 3302 3305 4343 4346 1186
Size: 1210 5 901 3 1038 3 3930
Fragments arranged by size:
3930 1210 1038 901 5 3 3
BseYI C'CCAG_C
Cuts at: 3522 4347 6293 6789 3522
Size: 825 1946 496 3823
Fragments arranged by size:
3823 1946 825 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 2576 3578 1163
Size: 573 828 12 1002 4675
Fragments arranged by size:
4675 1002 828 573 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 2129 4540 5301 6329 1540
Size: 275 186 65 63 2411 761 1028 2301
Fragments arranged by size:
2411 2301 1028 761 275 186 65 63
BsiHKAI G_wGCw'C
Cuts at: 2182 3397 3627 3765 5151 5236 6307 2182
Size: 1215 230 138 1386 85 1071 2965
Fragments arranged by size:
2965 1386 1215 1071 230 138 85
BsmI GAATG_Cn'
Cuts at: 2476 2649 2949 4601 4694 2476
Size: 173 300 1652 93 4872
Fragments arranged by size:
4872 1652 300 173 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2383 2383
Size: 7090
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2078 2182 2996 3111 3202 3208
Size: 399 245 43 104 814 115 91 6
Cuts at: 3208 3397 3627 3765 3946 4023 4409 5151 5236
Size: 189 230 138 181 77 386 742 85
Cuts at: 5236 6307 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 814 742 399 386 245 230
189 181 138 115 104 91 85 77
43 6
BspEI T'CCGG_A
Cuts at: 6753 6753
Size: 7090
BspHI T'CATG_A
Cuts at: 2457 3724 4979 6709 2457
Size: 1267 1255 1730 2838
Fragments arranged by size:
2838 1730 1267 1255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 2638 4445 844
Size: 1754 40 1807 3489
Fragments arranged by size:
3489 1807 1754 40
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 2425 3877 4977 1394
Size: 429 578 24 1452 1100 3507
Fragments arranged by size:
3507 1452 1100 578 429 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 3586 5585 5759 65
Size: 1183 2338 1999 174 1396
Fragments arranged by size:
2338 1999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3125 3420 5734 5921 1700
Size: 110 1315 295 2314 187 2869
Fragments arranged by size:
2869 2314 1315 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7090
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7090
BssSI C'ACGA_G
Cuts at: 2519 5150 6162 2519
Size: 2631 1012 3447
Fragments arranged by size:
3447 2631 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3671 794
Size: 2877 4213
BstBI TT'CG_AA
Cuts at: 5894 5894
Size: 7090
BstEII G'GTnAC_C
Cuts at: 1184 3964 1184
Size: 2780 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 3088 3088
Size: 7090
BstYI r'GATC_y
Cuts at: 1972 2724 3687 4420 4787 5172 5189 6641 6727
Size: 752 963 733 367 385 17 1452 86
Cuts at: 6727 6739 7085 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 963 752 733 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3956 4035 3956
Size: 79 7011
BtgI C'CryG_G
Cuts at: 513 1065 2004 2604 3093 3648 3984 4258 513
Size: 552 939 600 489 555 336 274 3345
Fragments arranged by size:
3345 939 600 555 552 489 336 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2431 3134 3550 3958 487
Size: 683 56 1205 703 416 408 3619
Fragments arranged by size:
3619 1205 703 683 416 408 56
BtsI GCAGTG_nn'
Cuts at: 2796 3038 4606 5400 5420 2796
Size: 242 1568 794 20 4466
Fragments arranged by size:
4466 1568 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 3225 1554
Size: 1671 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7055
DraI TTT'AAA
Cuts at: 4394 4394
Size: 7090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3425 6097 809
Size: 507 2109 2672 1802
Fragments arranged by size:
2672 2109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2063 2126
Size: 54 1425 50 275 18 177 56 63
Cuts at: 2126 2529 2799 2826 3078 3090 3123 4125 4537
Size: 403 270 27 252 12 33 1002 412
Cuts at: 4537 5426 7055 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 412 403 275 270
252 177 63 56 54 50 43 33
27 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2063 2126 4537 1537
Size: 275 251 63 2411 4090
Fragments arranged by size:
4090 2411 275 251 63
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 2373 2667 2984 3479 3857 4335 4365
Size: 1011 9 294 317 495 378 478 30
Cuts at: 4365 5020 1353
Size: 655 3423
Fragments arranged by size:
3423 1011 655 495 478 378 317 294
30 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5677 6051 6197 387
Size: 1064 138 438 3650 374 146 1280
Fragments arranged by size:
3650 1280 1064 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 2180 2180
Size: 7090
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2190 2388 2619 2712 2993 3008
Size: 393 478 80 198 231 93 281 15
Cuts at: 3008 3503 3512 3779 4060 5153 5750 6537 6805
Size: 495 9 267 281 1093 597 787 268
Cuts at: 6805 1239
Size: 1524
Fragments arranged by size:
1524 1093 787 597 495 478 393 281
281 268 267 231 198 93 80 15
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 3185 3695 3959 2162
Size: 1023 510 264 5293
Fragments arranged by size:
5293 1023 510 264
EcoO109I rG'GnC_Cy
Cuts at: 2057 2074 2155 3025 3205 4048 2057
Size: 17 81 870 180 843 5099
Fragments arranged by size:
5099 870 843 180 81 17
EcoRI G'AATT_C
Cuts at: 4399 4777 4399
Size: 378 6712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2696 2696
Size: 7090
FseI GG_CCGG'CC
Cuts at: 3129 3129
Size: 7090
FspI TGC'GCA
Cuts at: 2432 5596 2432
Size: 3164 3926
HaeII r_GCGC'y
Cuts at: 1678 2366 3021 3165 3256 4072 4187 6237 1678
Size: 688 655 144 91 816 115 2050 2531
Fragments arranged by size:
2531 2050 816 688 655 144 115 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5731 5763 5805 5837 1373
Size: 32 135 32 4159 32 42 32 2626
Fragments arranged by size:
4159 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4434 4680 669
Size: 988 281 2496 246 3079
Fragments arranged by size:
3079 2496 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 748
Size: 1930 5160
HpaI GTT'AAC
Cuts at: 4680 4680
Size: 7090
KasI G'GCGC_C
Cuts at: 2362 3017 4068 2362
Size: 655 1051 5384
Fragments arranged by size:
5384 1051 655
KpnI G_GTAC'C
Cuts at: 3339 4415 3339
Size: 1076 6014
MfeI C'AATT_G
Cuts at: 2195 4689 2195
Size: 2494 4596
MluI A'CGCG_T
Cuts at: 6966 6966
Size: 7090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3303 6204 6388 1305
Size: 1998 2901 184 2007
Fragments arranged by size:
2901 2007 1998 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 2801 3080 3092 4127
Size: 54 1425 343 699 270 279 12 1035
Cuts at: 4127 10
Size: 2973
Fragments arranged by size:
2973 1425 1035 699 343 279 270 54
12
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2894 3263 3620 5048 5407 5566
Size: 648 843 885 369 357 1428 359 159
Cuts at: 5566 518
Size: 2042
Fragments arranged by size:
2042 1428 885 843 648 369 359 357
159
MspA1I CmG'CkG
Cuts at: 1606 2068 2487 2690 3868 4506 5183 6331 6576
Size: 462 419 203 1178 638 677 1148 245
Cuts at: 6576 1606
Size: 2120
Fragments arranged by size:
2120 1178 1148 677 638 462 419 245
203
NaeI GCC'GGC
Cuts at: 3127 3127
Size: 7090
NarI GG'CG_CC
Cuts at: 2363 3018 4069 2363
Size: 655 1051 5384
Fragments arranged by size:
5384 1051 655
NcoI C'CATG_G
Cuts at: 513 1065 2004 3984 4258 513
Size: 552 939 1980 274 3345
Fragments arranged by size:
3345 1980 939 552 274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7090
NgoMIV G'CCGG_C
Cuts at: 3125 3125
Size: 7090
NheI G'CTAG_C
Cuts at: 1052 2138 2626 1052
Size: 1086 488 5516
Fragments arranged by size:
5516 1086 488
NotI GC'GGCC_GC
Cuts at: 4537 4537
Size: 7090
NspI r_CATG'y
Cuts at: 1132 1229 4448 5993 1132
Size: 97 3219 1545 2229
Fragments arranged by size:
3219 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3814 3892 1348
Size: 2466 78 4546
Fragments arranged by size:
4546 2466 78
PciI A'CATG_T
Cuts at: 5989 5989
Size: 7090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2808 3313 3529 3742 1108
Size: 1700 505 216 213 4456
Fragments arranged by size:
4456 1700 505 216 213
PfoI T'CCnGG_A
Cuts at: 2501 2501
Size: 7090
PmeI GTTT'AAAC
Cuts at: 4394 4394
Size: 7090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2991 3023 5159 5191 6672 6704 2991
Size: 32 2136 32 1481 32 3377
Fragments arranged by size:
3377 2136 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2155 3025 2057
Size: 98 870 6122
Fragments arranged by size:
6122 870 98
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7090
PsiI TTA'TAA
Cuts at: 4660 4660
Size: 7090
PspOMI G'GGCC_C
Cuts at: 2074 3204 4019 2074
Size: 1130 815 5145
Fragments arranged by size:
5145 1130 815
PstI C_TGCA'G
Cuts at: 830 2187 3453 4092 4161 4442 830
Size: 1357 1266 639 69 281 3478
Fragments arranged by size:
3478 1357 1266 639 281 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7090
PvuII CAG'CTG
Cuts at: 2690 3868 2690
Size: 1178 5912
SacI G_AGCT'C
Cuts at: 2182 2182
Size: 7090
SalI G'TCGA_C
Cuts at: 1936 4432 1936
Size: 2496 4594
SapI GCTCTTCn'nnn_
Cuts at: 2984 3479 4365 2984
Size: 495 886 5709
Fragments arranged by size:
5709 886 495
SbfI CC_TGCA'GG
Cuts at: 4442 4442
Size: 7090
ScaI AGT'ACT
Cuts at: 5338 7050 5338
Size: 1712 5378
SfcI C'TryA_G
Cuts at: 826 1045 2183 3449 4088 4135 4157 4438 5573
Size: 219 1138 1266 639 47 22 281 1135
Cuts at: 5573 6254 6445 826
Size: 681 191 1471
Fragments arranged by size:
1471 1266 1138 1135 681 639 281 219
191 47 22
SfoI GGC'GCC
Cuts at: 2364 3019 4070 2364
Size: 655 1051 5384
Fragments arranged by size:
5384 1051 655
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7090
SmaI CCC'GGG
Cuts at: 1872 2055 2116 3203 4417 1872
Size: 183 61 1087 1214 4545
Fragments arranged by size:
4545 1214 1087 183 61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2314 5194 6095 6357 820
Size: 197 926 371 2880 901 262 1553
Fragments arranged by size:
2880 1553 926 901 371 262 197
SnaBI TAC'GTA
Cuts at: 493 4881 493
Size: 4388 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7090
SphI G_CATG'C
Cuts at: 1132 4448 1132
Size: 3316 3774
SrfI GCCC'GGGC
Cuts at: 3203 3203
Size: 7090
SspI AAT'ATT
Cuts at: 5 52 2659 5014 5
Size: 47 2607 2355 2081
Fragments arranged by size:
2607 2355 2081 47
StuI AGG'CCT
Cuts at: 1517 2146 3156 4086 4133 1517
Size: 629 1010 930 47 4474
Fragments arranged by size:
4474 1010 930 629 47
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2715 3021 3081 3696 3741
Size: 552 939 528 183 306 60 615 45
Cuts at: 3741 3858 3984 4081 4128 4258 4386 4529 513
Size: 117 126 97 47 130 128 143 3074
Fragments arranged by size:
3074 939 615 552 528 306 183 143
130 128 126 117 97 60 47 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4352 5117 5134 5287 5472 5917 325
Size: 1255 2772 765 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 5336 7048
Size: 274 80 33 51 1443 792 2567 1712
Cuts at: 7048 96
Size: 138
Fragments arranged by size:
2567 1712 1443 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 2592 2624 7050 7082 2380
Size: 32 180 32 4426 32 2388
Fragments arranged by size:
4426 2388 180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2513 3347 3502 1316
Size: 1197 834 155 4904
Fragments arranged by size:
4904 1197 834 155
XbaI T'CTAG_A
Cuts at: 4426 4426
Size: 7090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2085 2274 2085
Size: 189 6901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7090
XmaI C'CCGG_G
Cuts at: 1870 2053 2114 3201 4415 1870
Size: 183 61 1087 1214 4545
Fragments arranged by size:
4545 1214 1087 183 61
XmnI GAAnn'nnTTC
Cuts at: 5219 5219
Size: 7090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4898 276
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI