Restriction map for
pFN21AB5845
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 396 GCGG CCGC(-3/-1)
AflIII 109 ACATGT A^C[GA][CT]GT
AlwI 189 GATCC GGATC(4/5)
AlwNI 317 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 280 CCTGAGG CC^T[ACGT]AGG
ApaI 129 GGGCCC GGGCC^C
AvaII 330 GGACC G^G[AT]CC
BanII 129 GGGCCC G[GA]GC[CT]^C
BcnI 219 CCGGG CC^[GC]GG
BseRI 103 GAGGAG GAGGAG(10/8)
BspLU11I 109 ACATGT A^CATGT
BspMI 392 ACCTGC ACCTGC(4/8)
BsrFI 332 ACCGGC [GA]^CCGG[CT]
BstYI 188 AGATCC [GA]^GATC[CT]
Bsu36I 280 CCTGAGG CC^T[ACGT]AGG
Cfr10I 332 ACCGGC [GA]^CCGG[CT]
DpnII 189 GATC ^GATC
EaeI 372 TGGCCG [CT]^GGCC[GA]
Eco81I 280 CCTGAGG CC^T[ACGT]AGG
FauI 396 GCGGG CCCGC(4/6)
HgaI 147 GACGC GACGC(5/10)
HinfI 54 GAATC G^A[ACGT]TC
MaeII 346 ACGT A^CGT
MboI 189 GATC ^GATC
MflI 188 AGATCC [GA]^GATC[CT]
MwoI 218 GCCGGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 219 CCGGG CC^[GC]GG
NdeII 189 GATC ^GATC
NlaIII 110 CATG CATG^
NspI 109 ACATGT [GA]CATG^[CT]
PflMI 6 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 184 CTGCAG CTGCA^G
Sau3AI 189 GATC ^GATC
SfaNI 355 GATGC GCATC(5/9)
SfcI 184 CTGCAG C^T[GA][CT]AG
TaqI 92 TCGA T^CGA
TfiI 54 GAATC G^A[AT]TC
TthHB8I 92 TCGA T^CGA
Van91I 6 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 192 CCAGTTTCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 188 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BmyI 129 GGGCCC G[AGT]GC[ACT]^C
243 GGGCAC
BpmI 210 CTCCAG CTGGAG(16/14)
151 CTGGAG
BsaJI 126 CCAGGG C^C[ACGT][ACGT]GG
219 CCGGGG
Bsp1286I 129 GGGCCC G[AGT]GC[ACT]^C
243 GGGCAC
DraII 128 AGGGCCC [GA]G^G[ACGT]CC[CT]
129 GGGCCCC
EcoO109I 128 AGGGCCC [GA]G^G[ACGT]CC[CT]
129 GGGCCCC
HaeIII 130 GGCC GG^CC
373 GGCC
HapII 219 CCGG C^CGG
333 CCGG
HpaII 219 CCGG C^CGG
333 CCGG
MaeIII 145 GTGAC ^GT[ACGT]AC
343 GTGAC
MspA1I 181 CAGCTG C[AC]G^C[GT]G
317 CAGCTG
MspI 219 CCGG C^CGG
333 CCGG
NlaIV 129 GGGCCC GG[ACGT]^[ACGT]CC
130 GGCCCC
PvuII 181 CAGCTG CAG^CTG
317 CAGCTG
Tsp45I 145 GTGAC ^GT[GC]AC
343 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI HphI
ItaI MnlI MvaI Sau96I ScrFI
TseI TspRI