(Circular) (Six-base) MAPSORT of: pFN21AB5733.seq Check: 5020 from: 1 to: 7087 pFN21AB5733 7087 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2290 4149 2290 Size: 1859 5228 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4897 278 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4430 1937 Size: 2493 4594 Acc65I G'GTAC_C Cuts at: 2370 4408 2370 Size: 2038 5049 AclI AA'CG_TT Cuts at: 1563 5214 5587 1563 Size: 3651 373 3063 Fragments arranged by size: 3651 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4317 5150 6534 6802 1632 Size: 2685 833 1384 268 1917 Fragments arranged by size: 2685 1917 1384 833 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6890 AflIII A'CryG_T Cuts at: 1203 2100 2328 5986 6963 1203 Size: 897 228 3658 977 1327 Fragments arranged by size: 3658 1327 977 897 228 AgeI A'CCGG_T Cuts at: 5918 5918 Size: 7087 AhdI GACnn_n'nnGTC Cuts at: 5816 5816 Size: 7087 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7087 AlwNI CAG_nnn'CTG Cuts at: 2290 3113 3762 3924 4830 6402 2290 Size: 823 649 162 906 1572 2975 Fragments arranged by size: 2975 1572 906 823 649 162 ApaI G_GGCC'C Cuts at: 2771 2771 Size: 7087 ApaLI G'TGCA_C Cuts at: 3082 5144 6300 3082 Size: 2062 1156 3869 Fragments arranged by size: 3869 2062 1156 ApoI r'AATT_y Cuts at: 1493 4396 4622 4774 6883 1493 Size: 2903 226 152 2109 1697 Fragments arranged by size: 2903 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5641 160 Size: 872 4609 1606 Fragments arranged by size: 4609 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 AvaI C'yCGr_G Cuts at: 1870 1943 2771 2881 3398 4412 1870 Size: 73 828 110 517 1014 4545 Fragments arranged by size: 4545 1014 828 517 110 73 BamHI G'GATC_C Cuts at: 3628 4417 4784 3628 Size: 789 367 5931 Fragments arranged by size: 5931 789 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2370 3101 3299 3533 3719 Size: 325 217 627 583 731 198 234 186 Cuts at: 3719 4408 5863 618 Size: 689 1455 1842 Fragments arranged by size: 1842 1455 731 689 627 583 325 234 217 198 186 BanII G_rGCy'C Cuts at: 1391 2343 2771 2864 3378 3438 4406 1391 Size: 952 428 93 514 60 968 4072 Fragments arranged by size: 4072 968 952 514 428 93 60 BbeI G_GCGC'C Cuts at: 3303 3303 Size: 7087 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2083 3416 4180 4192 928 Size: 971 184 1333 764 12 3823 Fragments arranged by size: 3823 1333 971 764 184 12 BbvCI CC'TCA_GC Cuts at: 2012 2361 3106 3983 4163 2012 Size: 349 745 877 180 4936 Fragments arranged by size: 4936 877 745 349 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3052 3086 4061 4095 5278 5312 1903 Size: 34 1115 34 975 34 1183 34 3678 Fragments arranged by size: 3678 1183 1115 975 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4979 6189 4979 Size: 1210 5877 BclI T'GATC_A Cuts at: 1548 1548 Size: 7087 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2819 3308 3531 Size: 107 122 71 631 605 1147 489 223 Cuts at: 3531 3852 4081 5698 136 Size: 321 229 1617 1525 Fragments arranged by size: 1617 1525 1147 631 605 489 321 229 223 122 107 71 BglII A'GATC_T Cuts at: 2181 7082 2181 Size: 4901 2186 BlpI GC'TnA_GC Cuts at: 4504 4504 Size: 7087 Bme1580I G_kGCm'C Cuts at: 1790 2439 2771 3086 3104 3536 4319 5148 6304 Size: 649 332 315 18 432 783 829 1156 Cuts at: 6304 1790 Size: 2573 Fragments arranged by size: 2573 1156 829 783 649 432 332 315 18 BmgBI CAC'GTC Cuts at: 1318 2329 3777 3901 1318 Size: 1011 1448 124 4504 Fragments arranged by size: 4504 1448 1011 124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3244 3604 4818 Size: 416 53 83 399 24 1827 360 1214 Cuts at: 4818 5776 442 Size: 958 1753 Fragments arranged by size: 1827 1753 1214 958 416 399 360 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2066 1056 Size: 1010 6077 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2292 2734 3019 3649 2292 Size: 442 285 630 5730 Fragments arranged by size: 5730 630 442 285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 3170 3476 3732 3834 5747 1239 Size: 903 1028 306 256 102 1913 2579 Fragments arranged by size: 2579 1913 1028 903 306 256 102 Bpu10I CC'TnA_GC Cuts at: 2012 2076 2361 3106 3121 3465 3983 4163 2012 Size: 64 285 745 15 344 518 180 4936 Fragments arranged by size: 4936 745 518 344 285 180 64 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2262 5212 6077 6375 2262 Size: 2950 865 298 2974 Fragments arranged by size: 2974 2950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2455 3899 5750 882 Size: 617 956 1444 1851 2219 Fragments arranged by size: 2219 1851 1444 956 617 BsaAI yAC'GTr Cuts at: 493 1204 2103 4878 493 Size: 711 899 2775 2702 Fragments arranged by size: 2775 2702 899 711 BsaBI GATnn'nnATC Cuts at: 3627 7081 3627 Size: 3454 3633 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3300 4894 5276 5911 Size: 53 83 186 936 1767 1594 382 635 Cuts at: 5911 275 Size: 1451 Fragments arranged by size: 1767 1594 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5520 5918 6192 6339 6750 5520 Size: 398 274 147 411 5857 Fragments arranged by size: 5857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4304 4334 1178 Size: 30 165 30 2901 30 3931 Fragments arranged by size: 3931 2901 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2229 2831 4325 1186 Size: 1043 602 1494 3948 Fragments arranged by size: 3948 1494 1043 602 BseYI C'CCAG_C Cuts at: 2128 2550 2722 2763 2857 3342 3992 6290 6786 Size: 422 172 41 94 485 650 2298 496 Cuts at: 6786 2128 Size: 2429 Fragments arranged by size: 2429 2298 650 496 485 422 172 94 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3803 1163 Size: 573 2067 4447 Fragments arranged by size: 4447 2067 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4537 5298 6326 1540 Size: 275 186 2536 761 1028 2301 Fragments arranged by size: 2536 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3086 3462 5148 5233 6304 3086 Size: 376 1686 85 1071 3869 Fragments arranged by size: 3869 1686 1071 376 85 BsmI GAATG_Cn' Cuts at: 4598 4691 4598 Size: 93 6994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 2439 2771 2864 3086 3104 3378 Size: 399 553 96 332 93 222 18 274 Cuts at: 3378 3438 3462 3536 4319 4406 5148 5233 6304 Size: 60 24 74 783 87 742 85 1071 Cuts at: 6304 1391 Size: 2174 Fragments arranged by size: 2174 1071 783 742 553 399 332 274 222 96 93 87 85 74 60 24 18 BspEI T'CCGG_A Cuts at: 6750 6750 Size: 7087 BspHI T'CATG_A Cuts at: 3603 4976 6706 3603 Size: 1373 1730 3984 Fragments arranged by size: 3984 1730 1373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 4149 4442 844 Size: 1446 1859 293 3489 Fragments arranged by size: 3489 1859 1446 293 BsrBI CCG'CTC Cuts at: 1394 1823 3540 4974 1394 Size: 429 1717 1434 3507 Fragments arranged by size: 3507 1717 1434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3135 5582 5756 65 Size: 1183 1887 2447 174 1396 Fragments arranged by size: 2447 1887 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 3128 3964 4157 5731 5918 1700 Size: 110 449 869 836 193 1574 187 2869 Fragments arranged by size: 2869 1574 869 836 449 193 187 110 BsrGI T'GTAC_A Cuts at: 96 4251 96 Size: 4155 2932 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7087 BssSI C'ACGA_G Cuts at: 5147 6159 5147 Size: 1012 6075 BstAPI GCAn_nnn'nTGC Cuts at: 794 2072 3762 4114 794 Size: 1278 1690 352 3767 Fragments arranged by size: 3767 1690 1278 352 BstBI TT'CG_AA Cuts at: 5891 5891 Size: 7087 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7087 BstXI CCAn_nnnn'nTGG Cuts at: 2146 3250 3275 3517 3550 2146 Size: 1104 25 242 33 5683 Fragments arranged by size: 5683 1104 242 33 25 BstYI r'GATC_y Cuts at: 1972 2181 3628 4417 4784 5169 5186 6638 6724 Size: 209 1447 789 367 385 17 1452 86 Cuts at: 6724 6736 7082 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1447 789 385 367 346 209 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3729 4146 3729 Size: 417 6670 BtgI C'CryG_G Cuts at: 513 1065 2004 2249 2830 2984 3303 3496 3528 Size: 552 939 245 581 154 319 193 32 Cuts at: 3528 4257 4264 513 Size: 729 7 3336 Fragments arranged by size: 3336 939 729 581 552 319 245 193 154 32 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6348 Fragments arranged by size: 6348 683 56 BtsI GCAGTG_nn' Cuts at: 2372 2712 3037 3763 3841 4603 5397 5417 2372 Size: 340 325 726 78 762 794 20 4042 Fragments arranged by size: 4042 794 762 726 340 325 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7087 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2267 2302 2953 2988 529 Size: 35 1703 35 651 35 4628 Fragments arranged by size: 4628 1703 651 35 35 35 DraI TTT'AAA Cuts at: 4391 4391 Size: 7087 DraIII CAC_nnn'GTG Cuts at: 2595 2597 3193 2595 Size: 2 596 6489 Fragments arranged by size: 6489 596 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2227 3775 6094 809 Size: 507 911 1548 2319 1802 Fragments arranged by size: 2319 1802 1548 911 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2619 3015 Size: 54 1425 50 275 18 306 483 396 Cuts at: 3015 3507 4155 4534 5423 7052 8 Size: 492 648 379 889 1629 43 Fragments arranged by size: 1629 1425 889 648 492 483 396 379 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4534 1537 Size: 275 2722 4090 Fragments arranged by size: 4090 2722 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3821 4326 5017 1353 Size: 2468 505 691 3423 Fragments arranged by size: 3423 2468 691 505 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5674 6048 6194 387 Size: 1064 138 4085 374 146 1280 Fragments arranged by size: 4085 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 3170 3476 3732 3834 4317 5150 Size: 393 510 1028 306 256 102 483 833 Cuts at: 5150 5747 6534 6802 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1028 833 787 597 510 483 393 306 268 256 102 EcoNI CCTnn'n_nnAGG Cuts at: 2813 4145 2813 Size: 1332 5755 EcoO109I rG'GnC_Cy Cuts at: 2016 2395 2539 2767 2768 3646 2016 Size: 379 144 228 1 878 5457 Fragments arranged by size: 5457 878 379 228 144 1 EcoRI G'AATT_C Cuts at: 4396 4774 4396 Size: 378 6709 FseI GG_CCGG'CC Cuts at: 3968 4161 3968 Size: 193 6894 FspI TGC'GCA Cuts at: 2114 2678 5593 2114 Size: 564 2915 3608 Fragments arranged by size: 3608 2915 564 FspAI rTGC'GCAy Cuts at: 2114 2678 2114 Size: 564 6523 HaeII r_GCGC'y Cuts at: 1678 2964 3303 4383 6234 1678 Size: 1286 339 1080 1851 2531 Fragments arranged by size: 2531 1851 1286 1080 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4304 4336 5728 5760 5802 Size: 32 135 32 2732 32 1392 32 42 Cuts at: 5802 5834 1373 Size: 32 2626 Fragments arranged by size: 2732 2626 1392 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4431 4677 669 Size: 988 281 2493 246 3079 Fragments arranged by size: 3079 2493 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7087 HpaI GTT'AAC Cuts at: 4677 4677 Size: 7087 KasI G'GCGC_C Cuts at: 3299 3299 Size: 7087 KpnI G_GTAC'C Cuts at: 2374 4412 2374 Size: 2038 5049 MfeI C'AATT_G Cuts at: 4686 4686 Size: 7087 MluI A'CGCG_T Cuts at: 6963 6963 Size: 7087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6201 6385 1305 Size: 4896 184 2007 Fragments arranged by size: 4896 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 2621 3017 3509 10 Size: 54 1425 343 306 483 396 492 3588 Fragments arranged by size: 3588 1425 492 483 396 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 3222 3273 5045 5404 5563 518 Size: 648 1401 655 51 1772 359 159 2042 Fragments arranged by size: 2042 1772 1401 655 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2069 2132 2251 2392 2763 2910 3346 3987 Size: 463 63 119 141 371 147 436 641 Cuts at: 3987 4167 4216 4503 5180 6328 6573 1606 Size: 180 49 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 463 436 371 287 245 180 147 141 119 63 49 NaeI GCC'GGC Cuts at: 2261 3130 3966 4159 2261 Size: 869 836 193 5189 Fragments arranged by size: 5189 869 836 193 NarI GG'CG_CC Cuts at: 3300 3300 Size: 7087 NcoI C'CATG_G Cuts at: 513 1065 2004 3303 3528 4257 513 Size: 552 939 1299 225 729 3343 Fragments arranged by size: 3343 1299 939 729 552 225 NdeI CA'TA_TG Cuts at: 387 3274 387 Size: 2887 4200 NgoMIV G'CCGG_C Cuts at: 2259 3128 3964 4157 2259 Size: 869 836 193 5189 Fragments arranged by size: 5189 869 836 193 NheI G'CTAG_C Cuts at: 1052 2062 1052 Size: 1010 6077 NotI GC'GGCC_GC Cuts at: 4534 4534 Size: 7087 NspI r_CATG'y Cuts at: 1132 1229 2683 3184 3957 4445 5990 1132 Size: 97 1454 501 773 488 1545 2229 Fragments arranged by size: 2229 1545 1454 773 501 488 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7087 PciI A'CATG_T Cuts at: 5986 5986 Size: 7087 PflMI CCAn_nnn'nTGG Cuts at: 1108 2103 3234 1108 Size: 995 1131 4961 Fragments arranged by size: 4961 1131 995 PmeI GTTT'AAAC Cuts at: 4391 4391 Size: 7087 PmlI CAC'GTG Cuts at: 2103 2103 Size: 7087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5156 5188 6669 6701 5156 Size: 32 1481 32 5542 Fragments arranged by size: 5542 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2539 3646 2395 Size: 144 1107 5836 Fragments arranged by size: 5836 1107 144 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7087 PsiI TTA'TAA Cuts at: 4657 4657 Size: 7087 PspOMI G'GGCC_C Cuts at: 2767 2767 Size: 7087 PspXI vC'TCGA_Gb Cuts at: 3398 3398 Size: 7087 PstI C_TGCA'G Cuts at: 830 2268 4215 4439 830 Size: 1438 1947 224 3478 Fragments arranged by size: 3478 1947 1438 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7087 PvuII CAG'CTG Cuts at: 2069 2763 2910 3987 4167 4216 2069 Size: 694 147 1077 180 49 4940 Fragments arranged by size: 4940 1077 694 180 147 49 SacII CC_GC'GG Cuts at: 2252 2252 Size: 7087 SalI G'TCGA_C Cuts at: 1936 4429 1936 Size: 2493 4594 SapI GCTCTTCn'nnn_ Cuts at: 3821 3821 Size: 7087 SbfI CC_TGCA'GG Cuts at: 4439 4439 Size: 7087 ScaI AGT'ACT Cuts at: 5335 7047 5335 Size: 1712 5375 SexAI A'CCwGG_T Cuts at: 3189 3516 3189 Size: 327 6760 SfcI C'TryA_G Cuts at: 826 1045 2264 2752 3025 3883 4211 4435 5570 Size: 219 1219 488 273 858 328 224 1135 Cuts at: 5570 6251 6442 826 Size: 681 191 1471 Fragments arranged by size: 1471 1219 1135 858 681 488 328 273 224 219 191 SfoI GGC'GCC Cuts at: 3301 3301 Size: 7087 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7087 SmaI CCC'GGG Cuts at: 1872 4414 1872 Size: 2542 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2277 3398 5191 6092 6354 820 Size: 197 926 334 1121 1793 901 262 1553 Fragments arranged by size: 1793 1553 1121 926 901 334 262 197 SnaBI TAC'GTA Cuts at: 493 4878 493 Size: 4385 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7087 SphI G_CATG'C Cuts at: 1132 2683 4445 1132 Size: 1551 1762 3774 Fragments arranged by size: 3774 1762 1551 SspI AAT'ATT Cuts at: 5 52 5011 5 Size: 47 4959 2081 Fragments arranged by size: 4959 2081 47 StuI AGG'CCT Cuts at: 1517 2822 2999 3788 1517 Size: 1305 177 789 4816 Fragments arranged by size: 4816 1305 789 177 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3303 3528 4005 4257 4526 Size: 552 939 48 1251 225 477 252 269 Cuts at: 4526 513 Size: 3074 Fragments arranged by size: 3074 1251 939 552 477 269 252 225 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2020 5114 5131 5284 5469 5914 325 Size: 1255 440 3094 17 153 185 445 1498 Fragments arranged by size: 3094 1498 1255 445 440 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2281 4251 5333 Size: 274 80 33 51 1443 304 1970 1082 Cuts at: 5333 7045 96 Size: 1712 138 Fragments arranged by size: 1970 1712 1443 1082 304 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7047 7079 7047 Size: 32 7055 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7087 XbaI T'CTAG_A Cuts at: 4423 4423 Size: 7087 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3247 3310 3247 Size: 63 7024 XhoI C'TCGA_G Cuts at: 1943 3398 1943 Size: 1455 5632 XmaI C'CCGG_G Cuts at: 1870 4412 1870 Size: 2542 4545 XmnI GAAnn'nnTTC Cuts at: 2090 5216 2090 Size: 3126 3961 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4895 276 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI NruI NsiI PacI PfoI PsrI RsrII SacI SanDI SfiI SrfI SwaI