Restriction map for
pFN21AB5733
(Linear) (Six-base) MAPSORT of: KIBB5733.seq Check: 5415 from: 1 to: 2382
KIBB5733.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 2144 2382
Size: 285 1859 238
Fragments arranged by size:
1859 285 238
Acc65I G'GTAC_C
Cuts at: 0 365 2382
Size: 365 2017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2312 2382
Size: 2312 70
AflIII A'CryG_T
Cuts at: 0 95 323 2382
Size: 95 228 2059
Fragments arranged by size:
2059 228 95
AlwNI CAG_nnn'CTG
Cuts at: 0 285 1108 1757 1919 2382
Size: 285 823 649 162 463
Fragments arranged by size:
823 649 463 285 162
ApaI G_GGCC'C
Cuts at: 0 766 2382
Size: 766 1616
ApaLI G'TGCA_C
Cuts at: 0 1077 2382
Size: 1077 1305
AvaI C'yCGr_G
Cuts at: 0 766 876 1393 2382
Size: 766 110 517 989
Fragments arranged by size:
989 766 517 110
BamHI G'GATC_C
Cuts at: 0 1623 2382
Size: 1623 759
BanI G'GyrC_C
Cuts at: 0 365 1096 1294 1528 1714 2382
Size: 365 731 198 234 186 668
Fragments arranged by size:
731 668 365 234 198 186
BanII G_rGCy'C
Cuts at: 0 338 766 859 1373 1433 2382
Size: 338 428 93 514 60 949
Fragments arranged by size:
949 514 428 338 93 60
BbeI G_GCGC'C
Cuts at: 0 1298 2382
Size: 1298 1084
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 1411 2175 2187 2382
Size: 78 1333 764 12 195
Fragments arranged by size:
1333 764 195 78 12
BbvCI CC'TCA_GC
Cuts at: 0 7 356 1101 1978 2158 2382
Size: 7 349 745 877 180 224
Fragments arranged by size:
877 745 349 224 180 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1047 1081 2056 2090 2382
Size: 1047 34 975 34 292
Fragments arranged by size:
1047 975 292 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 814 1303 1526 1847 2076 2382
Size: 814 489 223 321 229 306
Fragments arranged by size:
814 489 321 306 229 223
BglII A'GATC_T
Cuts at: 0 176 2382
Size: 176 2206
Bme1580I G_kGCm'C
Cuts at: 0 434 766 1081 1099 1531 2314 2382
Size: 434 332 315 18 432 783 68
Fragments arranged by size:
783 434 432 332 315 68 18
BmgBI CAC'GTC
Cuts at: 0 324 1772 1896 2382
Size: 324 1448 124 486
Fragments arranged by size:
1448 486 324 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 1239 1599 2382
Size: 1239 360 783
Fragments arranged by size:
1239 783 360
BmtI G_CTAG'C
Cuts at: 0 61 2382
Size: 61 2321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 287 729 1014 1644 2382
Size: 287 442 285 630 738
Fragments arranged by size:
738 630 442 287 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1165 1471 1727 1829 2382
Size: 137 1028 306 256 102 553
Fragments arranged by size:
1028 553 306 256 137 102
Bpu10I CC'TnA_GC
Cuts at: 0 7 71 356 1101 1116 1460 1978 2158
Size: 7 64 285 745 15 344 518 180
Cuts at: 2158 2382
Size: 224
Fragments arranged by size:
745 518 344 285 224 180 64 15
7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 2382
Size: 257 2125
BsaI GGTCTCn'nnnn_
Cuts at: 0 450 1894 2382
Size: 450 1444 488
Fragments arranged by size:
1444 488 450
BsaAI yAC'GTr
Cuts at: 0 98 2382
Size: 98 2284
BsaBI GATnn'nnATC
Cuts at: 0 1622 2382
Size: 1622 760
BsaHI Gr'CG_yC
Cuts at: 0 1295 2382
Size: 1295 1087
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2299 2329 2382
Size: 2299 30 53
Fragments arranged by size:
2299 53 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 224 826 2320 2382
Size: 224 602 1494 62
Fragments arranged by size:
1494 602 224 62
BseYI C'CCAG_C
Cuts at: 0 123 545 717 758 852 1337 1987 2382
Size: 123 422 172 41 94 485 650 395
Fragments arranged by size:
650 485 422 395 172 123 94 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1798 2382
Size: 1798 584
BsiHKAI G_wGCw'C
Cuts at: 0 1081 1457 2382
Size: 1081 376 925
Fragments arranged by size:
1081 925 376
Bsp1286I G_dGCh'C
Cuts at: 0 338 434 766 859 1081 1099 1373 1433
Size: 338 96 332 93 222 18 274 60
Cuts at: 1433 1457 1531 2314 2382
Size: 24 74 783 68
Fragments arranged by size:
783 338 332 274 222 96 93 74
68 60 24 18
BspHI T'CATG_A
Cuts at: 0 1598 2382
Size: 1598 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 2144 2382
Size: 285 1859 238
Fragments arranged by size:
1859 285 238
BsrBI CCG'CTC
Cuts at: 0 1535 2382
Size: 1535 847
BsrDI GCAATG_nn'
Cuts at: 0 1130 2382
Size: 1130 1252
BsrFI r'CCGG_y
Cuts at: 0 254 1123 1959 2152 2382
Size: 254 869 836 193 230
Fragments arranged by size:
869 836 254 230 193
BsrGI T'GTAC_A
Cuts at: 0 2246 2382
Size: 2246 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 67 1757 2109 2382
Size: 67 1690 352 273
Fragments arranged by size:
1690 352 273 67
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 1245 1270 1512 1545 2382
Size: 141 1104 25 242 33 837
Fragments arranged by size:
1104 837 242 141 33 25
BstYI r'GATC_y
Cuts at: 0 176 1623 2382
Size: 176 1447 759
Fragments arranged by size:
1447 759 176
Bsu36I CC'TnA_GG
Cuts at: 0 1724 2141 2382
Size: 1724 417 241
Fragments arranged by size:
1724 417 241
BtgI C'CryG_G
Cuts at: 0 244 825 979 1298 1491 1523 2252 2259
Size: 244 581 154 319 193 32 729 7
Cuts at: 2259 2382
Size: 123
Fragments arranged by size:
729 581 319 244 193 154 123 32
7
BtsI GCAGTG_nn'
Cuts at: 0 367 707 1032 1758 1836 2382
Size: 367 340 325 726 78 546
Fragments arranged by size:
726 546 367 340 325 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 262 297 948 983 2382
Size: 262 35 651 35 1399
Fragments arranged by size:
1399 651 262 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 590 592 1188 2382
Size: 590 2 596 1194
Fragments arranged by size:
1194 596 590 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 222 1770 2382
Size: 222 1548 612
Fragments arranged by size:
1548 612 222
EaeI y'GGCC_r
Cuts at: 0 131 614 1010 1502 2150 2382
Size: 131 483 396 492 648 232
Fragments arranged by size:
648 492 483 396 232 131
EarI CTCTTCn'nnn_
Cuts at: 0 1816 2321 2382
Size: 1816 505 61
Fragments arranged by size:
1816 505 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1165 1471 1727 1829 2312 2382
Size: 137 1028 306 256 102 483 70
Fragments arranged by size:
1028 483 306 256 137 102 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 2140 2382
Size: 808 1332 242
Fragments arranged by size:
1332 808 242
EcoO109I rG'GnC_Cy
Cuts at: 0 11 390 534 762 763 1641 2382
Size: 11 379 144 228 1 878 741
Fragments arranged by size:
878 741 379 228 144 11 1
FseI GG_CCGG'CC
Cuts at: 0 1963 2156 2382
Size: 1963 193 226
Fragments arranged by size:
1963 226 193
FspI TGC'GCA
Cuts at: 0 109 673 2382
Size: 109 564 1709
Fragments arranged by size:
1709 564 109
FspAI rTGC'GCAy
Cuts at: 0 109 673 2382
Size: 109 564 1709
Fragments arranged by size:
1709 564 109
HaeII r_GCGC'y
Cuts at: 0 959 1298 2378 2382
Size: 959 339 1080 4
Fragments arranged by size:
1080 959 339 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2299 2331 2382
Size: 2299 32 51
Fragments arranged by size:
2299 51 32
KasI G'GCGC_C
Cuts at: 0 1294 2382
Size: 1294 1088
KpnI G_GTAC'C
Cuts at: 0 369 2382
Size: 369 2013
MscI TGG'CCA
Cuts at: 0 133 616 1012 1504 2382
Size: 133 483 396 492 878
Fragments arranged by size:
878 492 483 396 133
MslI CAynn'nnrTG
Cuts at: 0 562 1217 1268 2382
Size: 562 655 51 1114
Fragments arranged by size:
1114 655 562 51
MspA1I CmG'CkG
Cuts at: 0 64 127 246 387 758 905 1341 1982
Size: 64 63 119 141 371 147 436 641
Cuts at: 1982 2162 2211 2382
Size: 180 49 171
Fragments arranged by size:
641 436 371 180 171 147 141 119
64 63 49
NaeI GCC'GGC
Cuts at: 0 256 1125 1961 2154 2382
Size: 256 869 836 193 228
Fragments arranged by size:
869 836 256 228 193
NarI GG'CG_CC
Cuts at: 0 1295 2382
Size: 1295 1087
NcoI C'CATG_G
Cuts at: 0 1298 1523 2252 2382
Size: 1298 225 729 130
Fragments arranged by size:
1298 729 225 130
NdeI CA'TA_TG
Cuts at: 0 1269 2382
Size: 1269 1113
NgoMIV G'CCGG_C
Cuts at: 0 254 1123 1959 2152 2382
Size: 254 869 836 193 230
Fragments arranged by size:
869 836 254 230 193
NheI G'CTAG_C
Cuts at: 0 57 2382
Size: 57 2325
NspI r_CATG'y
Cuts at: 0 678 1179 1952 2382
Size: 678 501 773 430
Fragments arranged by size:
773 678 501 430
PflMI CCAn_nnn'nTGG
Cuts at: 0 98 1229 2382
Size: 98 1131 1153
Fragments arranged by size:
1153 1131 98
PmlI CAC'GTG
Cuts at: 0 98 2382
Size: 98 2284
PpuMI rG'GwC_Cy
Cuts at: 0 390 534 1641 2382
Size: 390 144 1107 741
Fragments arranged by size:
1107 741 390 144
PspOMI G'GGCC_C
Cuts at: 0 762 2382
Size: 762 1620
PspXI vC'TCGA_Gb
Cuts at: 0 1393 2382
Size: 1393 989
PstI C_TGCA'G
Cuts at: 0 263 2210 2382
Size: 263 1947 172
Fragments arranged by size:
1947 263 172
PvuII CAG'CTG
Cuts at: 0 64 758 905 1982 2162 2211 2382
Size: 64 694 147 1077 180 49 171
Fragments arranged by size:
1077 694 180 171 147 64 49
SacII CC_GC'GG
Cuts at: 0 247 2382
Size: 247 2135
SapI GCTCTTCn'nnn_
Cuts at: 0 1816 2382
Size: 1816 566
SexAI A'CCwGG_T
Cuts at: 0 1184 1511 2382
Size: 1184 327 871
Fragments arranged by size:
1184 871 327
SfcI C'TryA_G
Cuts at: 0 259 747 1020 1878 2206 2382
Size: 259 488 273 858 328 176
Fragments arranged by size:
858 488 328 273 259 176
SfoI GGC'GCC
Cuts at: 0 1296 2382
Size: 1296 1086
SmlI C'TyrA_G
Cuts at: 0 272 1393 2382
Size: 272 1121 989
Fragments arranged by size:
1121 989 272
SphI G_CATG'C
Cuts at: 0 678 2382
Size: 678 1704
StuI AGG'CCT
Cuts at: 0 817 994 1783 2382
Size: 817 177 789 599
Fragments arranged by size:
817 789 599 177
StyI C'CwwG_G
Cuts at: 0 47 1298 1523 2000 2252 2382
Size: 47 1251 225 477 252 130
Fragments arranged by size:
1251 477 252 225 130 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 15 2382
Size: 15 2367
TatI w'GTAC_w
Cuts at: 0 276 2246 2382
Size: 276 1970 136
Fragments arranged by size:
1970 276 136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1242 1305 2382
Size: 1242 63 1077
Fragments arranged by size:
1242 1077 63
XhoI C'TCGA_G
Cuts at: 0 1393 2382
Size: 1393 989
XmnI GAAnn'nnTTC
Cuts at: 0 85 2382
Size: 85 2297
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI
DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I FseI
FspI FspAI HaeII Hin4I KasI KpnI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PflMI PmlI PpuMI PspOMI PspXI PstI PvuII SacII
SapI SexAI SfcI SfoI SmlI SphI StuI StyI
TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BlpI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI DraI EagI EciI EcoICRI EcoRI EcoRV FalI
HincII HindIII HpaI MfeI MluI MmeI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XbaI XmaI ZraI