Restriction map for
pFN21AB5841
(Circular) (Six-base) MAPSORT of: pFN21AB5841.seq Check: 7322 from: 1 to: 5914
pFN21AB5841 5914 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2881 2881
Size: 5914
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2248 2433 2489 3032 3977 5361 5629 1632
Size: 616 185 56 543 945 1384 268 1917
Fragments arranged by size:
1917 1384 945 616 543 268 185 56
AfeI AGC'GCT
Cuts at: 2507 2507
Size: 5914
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 2563 4643 2563
Size: 2080 3834
AleI CACnn'nnGTG
Cuts at: 1166 2458 1166
Size: 1292 4622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3128 1184
Size: 1944 3970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2648 2680 2279
Size: 32 337 32 5513
Fragments arranged by size:
5513 337 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2837 3657 5229 2837
Size: 820 1572 3522
Fragments arranged by size:
3522 1572 820
ApaI G_GGCC'C
Cuts at: 2097 2636 2831 2097
Size: 539 195 5180
Fragments arranged by size:
5180 539 195
ApaLI G'TGCA_C
Cuts at: 2442 3971 5127 2442
Size: 1529 1156 3229
Fragments arranged by size:
3229 1529 1156
ApoI r'AATT_y
Cuts at: 1493 3223 3449 3601 5710 1493
Size: 1730 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2435 2435
Size: 5914
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 3239 1870
Size: 73 570 726 4545
Fragments arranged by size:
4545 726 570 73
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2394 2552 2728 2812
Size: 325 217 627 468 139 158 176 84
Cuts at: 2812 3235 4690 618
Size: 423 1455 1842
Fragments arranged by size:
1842 1455 627 468 423 325 217 176
158 139 84
BanII G_rGCy'C
Cuts at: 1391 2097 2361 2636 2831 3081 3233 1391
Size: 706 264 275 195 250 152 4072
Fragments arranged by size:
4072 706 275 264 250 195 152
BbeI G_GCGC'C
Cuts at: 2556 2816 2556
Size: 260 5654
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3155 4525 136
Size: 107 122 71 631 605 1483 1370 1525
Fragments arranged by size:
1525 1483 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2397 2446 2636 2831 3205 3975 5131
Size: 307 300 49 190 195 374 770 1156
Cuts at: 5131 1790
Size: 2573
Fragments arranged by size:
2573 1156 770 374 307 300 195 190
49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2504 2522 3209 3227 4574 1239
Size: 1229 36 18 687 18 1347 2579
Fragments arranged by size:
2579 1347 1229 687 36 18 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 2300 4577 882
Size: 617 645 156 2277 2219
Fragments arranged by size:
2277 2219 645 617 156
BsaAI yAC'GTr
Cuts at: 493 1204 2182 2246 2602 2644 2773 2935 3705
Size: 711 978 64 356 42 129 162 770
Cuts at: 3705 493
Size: 2702
Fragments arranged by size:
2702 978 770 711 356 162 129 64
42
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 2685 2813 3721
Size: 53 83 186 936 1020 132 128 908
Cuts at: 3721 4103 4738 275
Size: 382 635 1451
Fragments arranged by size:
1451 1020 936 908 635 382 186 132
128 83 53
BsaWI w'CCGG_w
Cuts at: 2277 4347 4745 5019 5166 5577 2277
Size: 2070 398 274 147 411 2614
Fragments arranged by size:
2614 2070 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 2481 2511 1178
Size: 30 165 30 876 30 172 30 4581
Fragments arranged by size:
4581 876 172 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4656
BseYI C'CCAG_C
Cuts at: 2540 2956 3129 5117 5613 2540
Size: 416 173 1988 496 2841
Fragments arranged by size:
2841 1988 496 416 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2823 2830 1163
Size: 573 1087 7 4247
Fragments arranged by size:
4247 1087 573 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2373 3364 4125 5153 1540
Size: 275 186 372 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 372 275 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2446 3213 3975 4060 5131 2361
Size: 85 767 762 85 1071 3144
Fragments arranged by size:
3144 1071 767 762 85 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2361 2397 2446 2636 2831 3081
Size: 399 307 264 36 49 190 195 250
Cuts at: 3081 3205 3213 3233 3975 4060 5131 1391
Size: 124 8 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 307 264 250 195
190 124 85 49 36 20 8
BspEI T'CCGG_A
Cuts at: 2277 5577 2277
Size: 3300 2614
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 2881 3269 844
Size: 1691 346 388 3489
Fragments arranged by size:
3489 1691 388 346
BsrBI CCG'CTC
Cuts at: 1394 1823 2822 3801 1394
Size: 429 999 979 3507
Fragments arranged by size:
3507 999 979 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2478 4558 4745 1700
Size: 110 668 2080 187 2869
Fragments arranged by size:
2869 2080 668 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 2435 1925
Size: 510 5404
BssSI C'ACGA_G
Cuts at: 2458 3974 4986 2458
Size: 1516 1012 3386
Fragments arranged by size:
3386 1516 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 4529
BstYI r'GATC_y
Cuts at: 1972 3244 3611 3996 4013 5465 5551 5563 5909
Size: 1272 367 385 17 1452 86 12 346
Cuts at: 5909 1972
Size: 1977
Fragments arranged by size:
1977 1452 1272 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2018 2043 2018
Size: 25 5889
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 513
Size: 552 939 684 3739
Fragments arranged by size:
3739 939 684 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 2900 3430 4224 4244 2900
Size: 530 794 20 4570
Fragments arranged by size:
4570 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 2649 1554
Size: 1095 4819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DraIII CAC_nnn'GTG
Cuts at: 2030 2030
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2690 4921 809
Size: 507 1374 2231 1802
Fragments arranged by size:
2231 1802 1374 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 2999 3361
Size: 54 1425 50 275 18 591 578 362
Cuts at: 3361 4250 5879 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 591 578 362 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2919 3143 3188 3844 1353
Size: 1566 224 45 656 3423
Fragments arranged by size:
3423 1566 656 224 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2359
Size: 5914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2248 2433 2468 2489 2504 2522 3032
Size: 393 616 185 35 21 15 18 510
Cuts at: 3032 3209 3227 3977 4574 5361 5629 1239
Size: 177 18 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 616 597 510 393 268
185 177 35 21 18 18 15
EcoO109I rG'GnC_Cy
Cuts at: 2093 2094 2632 2633 2834 2093
Size: 1 538 1 201 5173
Fragments arranged by size:
5173 538 201 1 1
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 5914
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 2509 2556 2726 2816 5061 1678
Size: 831 47 170 90 2245 2531
Fragments arranged by size:
2531 2245 831 170 90 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 4555 4587 4629
Size: 32 135 32 977 32 1974 32 42
Cuts at: 4629 4661 1373
Size: 32 2626
Fragments arranged by size:
2626 1974 977 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2695 2965 3258 3504 669
Size: 988 281 757 270 293 246 3079
Fragments arranged by size:
3079 988 757 293 281 270 246
HindIII A'AGCT_T
Cuts at: 748 3044 748
Size: 2296 3618
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KasI G'GCGC_C
Cuts at: 2552 2812 2552
Size: 260 5654
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3043 5028 5212 1305
Size: 1738 1985 184 2007
Fragments arranged by size:
2007 1985 1738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 3001 10
Size: 54 1425 343 591 578 2923
Fragments arranged by size:
2923 1425 591 578 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2067 2458 3872 4231 4390 518
Size: 648 901 391 1414 359 159 2042
Fragments arranged by size:
2042 1414 901 648 391 359 159
MspA1I CmG'CkG
Cuts at: 1606 2544 2866 2960 3330 4007 5155 5400 1606
Size: 938 322 94 370 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 370 322 245 94
NaeI GCC'GGC
Cuts at: 2480 2480
Size: 5914
NarI GG'CG_CC
Cuts at: 2553 2813 2553
Size: 260 5654
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NgoMIV G'CCGG_C
Cuts at: 2478 2478
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 3272 4817 1132
Size: 97 2043 1545 2229
Fragments arranged by size:
2229 2043 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2232 1348
Size: 884 5030
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 2983 3062 1108
Size: 1074 801 79 3960
Fragments arranged by size:
3960 1074 801 79
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PmlI CAC'GTG
Cuts at: 2182 2246 2602 2182
Size: 64 356 5494
Fragments arranged by size:
5494 356 64
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2311 3983 4015 5496 5528 2279
Size: 32 1672 32 1481 32 2665
Fragments arranged by size:
2665 1672 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2834 2834
Size: 5914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 2093 2632 2827 2093
Size: 539 195 5180
Fragments arranged by size:
5180 539 195
PstI C_TGCA'G
Cuts at: 830 2136 3266 830
Size: 1306 1130 3478
Fragments arranged by size:
3478 1306 1130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2960 2960
Size: 5914
SacI G_AGCT'C
Cuts at: 2361 2361
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 2136 3266 2136
Size: 1130 4784
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2132 3176 3262 4397 5078 5269 826
Size: 219 1087 1044 86 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 1044 681 219 191 86
SfoI GGC'GCC
Cuts at: 2554 2814 2554
Size: 260 5654
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 2130 1517
Size: 613 5301
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 3353 513
Size: 552 939 306 1043 3074
Fragments arranged by size:
3074 1043 939 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 2387 2557 2797 3941 3958 4111
Size: 1255 693 114 170 240 1144 17 153
Cuts at: 4111 4296 4741 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1144 693 445 240 185 170
153 114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 2481 2513 5874 5906 2016
Size: 32 433 32 3361 32 2024
Fragments arranged by size:
3361 2024 433 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 2080 3250 2080
Size: 1170 4744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 3151 2722
Size: 429 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 2336 4043 2336
Size: 1707 4207
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI
BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI