(Circular) (Six-base) MAPSORT of: pFN21AB5840.seq Check: 2110 from: 1 to: 6151 pFN21AB5840 6151 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 3356 2192 Size: 1164 4987 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 3414 4214 5598 5866 1632 Size: 954 828 800 1384 268 1917 Fragments arranged by size: 1917 1384 954 828 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 2715 5050 6027 1203 Size: 1512 2335 977 1327 Fragments arranged by size: 2335 1512 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 2734 3392 3894 5466 2734 Size: 658 502 1572 3419 Fragments arranged by size: 3419 1572 658 502 ApaI G_GGCC'C Cuts at: 3244 3244 Size: 6151 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2461 3460 3686 3838 5947 1493 Size: 968 999 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 968 226 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 2904 3481 3848 2904 Size: 577 367 5207 Fragments arranged by size: 5207 577 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2585 2811 3244 3470 1391 Size: 1194 226 433 226 4072 Fragments arranged by size: 4072 1194 433 226 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BfrBI ATG'CAT Cuts at: 2200 2200 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 2964 3568 2964 Size: 604 5547 Bme1580I G_kGCm'C Cuts at: 1790 2648 3244 4212 5368 1790 Size: 858 596 968 1156 2573 Fragments arranged by size: 2573 1156 968 858 596 BmgBI CAC'GTC Cuts at: 1318 3134 1318 Size: 1816 4335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2509 2880 3052 Size: 416 53 83 399 24 1092 371 172 Cuts at: 3052 3882 4840 442 Size: 830 958 1753 Fragments arranged by size: 1753 1092 958 830 416 399 371 172 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4811 1239 Size: 3572 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2383 2921 4276 5141 5439 2383 Size: 538 1355 865 298 3095 Fragments arranged by size: 3095 1355 865 538 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2301 4814 882 Size: 617 802 2513 2219 Fragments arranged by size: 2513 2219 802 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2971 3065 3212 1186 Size: 1785 94 147 4125 Fragments arranged by size: 4125 1785 147 94 BseYI C'CCAG_C Cuts at: 3334 5354 5850 3334 Size: 2020 496 3635 Fragments arranged by size: 3635 2020 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2545 2843 3133 1163 Size: 573 809 298 290 4181 Fragments arranged by size: 4181 809 573 298 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 4212 4297 5368 2811 Size: 1401 85 1071 3594 Fragments arranged by size: 3594 1401 1071 85 BsmI GAATG_Cn' Cuts at: 2512 2943 3008 3662 3755 2512 Size: 431 65 654 93 4908 Fragments arranged by size: 4908 654 431 93 65 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2585 2648 2811 3244 3470 4212 4297 Size: 399 795 63 163 433 226 742 85 Cuts at: 4297 5368 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 795 742 433 399 226 163 85 63 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 3415 4040 5770 3415 Size: 625 1730 3796 Fragments arranged by size: 3796 1730 625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2192 2477 3105 3356 3506 844 Size: 1334 14 285 628 251 150 3489 Fragments arranged by size: 3489 1334 628 285 251 150 14 BsrBI CCG'CTC Cuts at: 1394 1823 2057 4038 1394 Size: 429 234 1981 3507 Fragments arranged by size: 3507 1981 429 234 BsrDI GCAATG_nn' Cuts at: 65 1248 3102 4646 4820 65 Size: 1183 1854 1544 174 1396 Fragments arranged by size: 1854 1544 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 2734 794 Size: 1940 4211 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 2179 1184 Size: 995 5156 BstYI r'GATC_y Cuts at: 1972 2850 2904 3445 3481 3848 4233 4250 5702 Size: 878 54 541 36 367 385 17 1452 Cuts at: 5702 5788 5800 6146 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 878 541 385 367 346 86 54 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2622 2698 513 Size: 552 939 618 76 3966 Fragments arranged by size: 3966 939 618 552 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3667 4461 4481 2664 Size: 1003 794 20 4334 Fragments arranged by size: 4334 1003 794 20 ClaI AT'CG_AT Cuts at: 1554 2411 1554 Size: 857 5294 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2542 3187 3598 Size: 54 1425 50 275 18 712 645 411 Cuts at: 3598 4487 6116 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 712 645 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3054 3309 4081 1353 Size: 1701 255 772 3423 Fragments arranged by size: 3423 1701 772 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2145 4738 5112 5258 387 Size: 1064 138 435 121 2593 374 146 1280 Fragments arranged by size: 2593 1280 1064 435 374 146 138 121 EcoICRI GAG'CTC Cuts at: 2809 2809 Size: 6151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2586 3414 4214 4811 5598 5866 1239 Size: 393 954 828 800 597 787 268 1524 Fragments arranged by size: 1524 954 828 800 787 597 393 268 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2896 5298 1678 Size: 1218 2402 2531 Fragments arranged by size: 2531 2402 1218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4792 4824 4866 4898 1373 Size: 32 135 32 3220 32 42 32 2626 Fragments arranged by size: 3220 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2398 2937 5265 5449 1305 Size: 1093 539 2328 184 2007 Fragments arranged by size: 2328 2007 1093 539 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 4109 4468 4627 518 Size: 648 1695 1248 359 159 2042 Fragments arranged by size: 2042 1695 1248 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2157 2313 2641 3567 4244 5392 5637 Size: 457 94 156 328 926 677 1148 245 Cuts at: 5637 1606 Size: 2120 Fragments arranged by size: 2120 1148 926 677 457 328 245 156 94 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NsiI A_TGCA'T Cuts at: 2202 2202 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2719 3176 3509 5054 1132 Size: 97 1490 457 333 1545 2229 Fragments arranged by size: 2229 1545 1490 457 333 97 PasI CC'CwG_GG Cuts at: 1348 2119 1348 Size: 771 5380 PciI A'CATG_T Cuts at: 2715 5050 2715 Size: 2335 3816 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6151 PfoI T'CCnGG_A Cuts at: 2023 2023 Size: 6151 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2226 1613 Size: 613 5538 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PspOMI G'GGCC_C Cuts at: 3240 3240 Size: 6151 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3149 3181 3149 Size: 32 6119 PstI C_TGCA'G Cuts at: 830 2189 2318 2580 2659 2730 3367 3503 830 Size: 1359 129 262 79 71 637 136 3478 Fragments arranged by size: 3478 1359 637 262 136 129 79 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2641 2641 Size: 6151 SacI G_AGCT'C Cuts at: 2811 2811 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SbfI CC_TGCA'GG Cuts at: 2189 2580 3503 2189 Size: 391 923 4837 Fragments arranged by size: 4837 923 391 ScaI AGT'ACT Cuts at: 3041 4399 6111 3041 Size: 1358 1712 3081 Fragments arranged by size: 3081 1712 1358 SfcI C'TryA_G Cuts at: 826 1045 2185 2314 2361 2478 2576 2655 2726 Size: 219 1140 129 47 117 98 79 71 Cuts at: 2726 3363 3499 4634 5315 5506 826 Size: 637 136 1135 681 191 1471 Fragments arranged by size: 1471 1140 1135 681 637 219 191 136 129 117 98 79 71 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2398 2936 4255 5156 5418 820 Size: 197 926 455 538 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 538 455 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3176 3509 1132 Size: 2044 333 3774 Fragments arranged by size: 3774 2044 333 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 3590 513 Size: 552 939 588 998 3074 Fragments arranged by size: 3074 998 939 588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2491 3034 4178 4195 4348 4533 4978 Size: 1255 911 543 1144 17 153 185 445 Cuts at: 4978 325 Size: 1498 Fragments arranged by size: 1498 1255 1144 911 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3039 4397 6109 Size: 274 80 33 51 1443 1062 1358 1712 Cuts at: 6109 96 Size: 138 Fragments arranged by size: 1712 1443 1358 1062 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 6111 6143 2828 Size: 32 3251 32 2836 Fragments arranged by size: 3251 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3039 3039 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 3257 4280 3257 Size: 1023 5128 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI