Restriction map for
pFN21AB5839
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BpmI
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstYI BstZ17I Cac8I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI HapII HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 460 GACGCC G[GA]^CG[CT]C
AfaI 405 GTAC GT^AC
AocI 334 CCTAAGG CC^T[ACGT]AGG
Aor51HI 371 AGCGCT AGC^GCT
AvaII 360 GGACC G^G[AT]CC
AviII 281 TGCGCA TGC^GCA
AvrII 219 CCTAGG C^CTAGG
BclI 497 TGATCA T^GATCA
BlnI 219 CCTAGG C^CTAGG
BlpI 468 GCTGAGC GC^T[ACGT]AGC
BmyI 167 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
BsaHI 460 GACGCC G[GA]^CG[CT]C
BsaI 206 GAGACC GGTCTC(1/5)
BsgI 489 CTGCAC GTGCAG(16/14)
BsmFI 593 GGGAC GGGAC(10/14)
Bsp1286I 167 GTGCCC G[AGT]GC[ACT]^C
BsrDI 260 GCAATG GCAATG(2/0)
BstBI 317 TTCGAA TT^CGAA
Bsu36I 334 CCTAAGG CC^T[ACGT]AGG
CelII 468 GCTGAGC GC^T[ACGT]AGC
DraII 237 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 179 CCATGG C^C[GA][CT]GG
EaeI 533 TGGCCG [CT]^GGCC[GA]
Eco47III 371 AGCGCT AGC^GCT
Eco81I 334 CCTAAGG CC^T[ACGT]AGG
EcoO109I 237 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 497 TGATCA T^GATCA
FspI 281 TGCGCA TGC^GCA
HgaI 460 GACGC GACGC(5/10)
Hin1I 460 GACGCC G[GA]^CG[CT]C
MaeIII 7 GTGAC ^GT[ACGT]AC
MslI 291 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 169 GCCCTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 179 CCATGG C^CATGG
NspV 317 TTCGAA TT^CGAA
PstI 55 CTGCAG CTGCA^G
RsaI 405 GTAC GT^AC
ScaI 404 AGTACT AGT^ACT
SfaNI 377 GCATC GCATC(5/9)
SfuI 317 TTCGAA TT^CGAA
TaqI 318 TCGA T^CGA
TfiI 315 GATTC G^A[AT]TC
Tsp45I 7 GTGAC ^GT[GC]AC
TthHB8I 318 TCGA T^CGA
XbaI 432 TCTAGA T^CTAGA
XcmI 579 CCACAGCAACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BfaI 220 CTAG C^TAG
433 CTAG
BsmAI 206 GAGAC GTCTC(1/5)
352 GTCTC
BsrI 186 CCAGT ACTGG(1/-1)
306 CCAGT
BssKI 171 CCTGG ^CC[ACGT]GG
234 CCTGG
BstF5I 339 GGATG GGATG(2/0)
456 GGATG
BstNI 171 CCTGG CC^[AT]GG
234 CCTGG
DpnII 430 GATC ^GATC
498 GATC
EcoRII 171 CCTGG ^CC[AT]GG
234 CCTGG
EcoT14I 179 CCATGG C^C[AT][AT]GG
219 CCTAGG
FokI 339 GGATG GGATG(9/13)
456 GGATG
HaeII 213 AGCGCC [GA]GCGC^[CT]
371 AGCGCT
HphI 6 GGTGA GGTGA(8/7)
567 GGTGA
MaeI 220 CTAG C^TAG
433 CTAG
MboI 430 GATC ^GATC
498 GATC
MvaI 171 CCTGG CC^[AT]GG
234 CCTGG
NdeII 430 GATC ^GATC
498 GATC
NlaIII 180 CATG CATG^
279 CATG
PleI 24 GAGTC GAGTC(4/5)
538 GAGTC
Sau3AI 430 GATC ^GATC
498 GATC
ScrFI 171 CCTGG CC^[ACGT]GG
234 CCTGG
StyI 179 CCATGG C^C[AT][AT]GG
219 CCTAGG
TspRI 187 CAGTG CA[GC]TG[ACGT][ACGT]^
307 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbsI BbvI BpuAI
BsaJI CfoI Cfr13I DdeI Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MboII MnlI NlaIV Sau96I SfcI
TseI